Click here to switch to the map view.

The map label for this gene is kdsB

Identifier: 146302240

GI number: 146302240

Start: 5453881

End: 5454615

Strand: Reverse

Name: kdsB

Synonym: Fjoh_4513

Alternate gene names: 146302240

Gene position: 5454615-5453881 (Counterclockwise)

Preceding gene: 146302242

Following gene: 146302231

Centisome position: 89.47

GC content: 35.78

Gene sequence:

>735_bases
ATGAAAATAATAGCGGTAATACCGGCACGATATGCCTCAACACGATTTCCTGCAAAATTGATGCAGGATTTGGGAGGTAA
AACAGTAATTCTAAGAACGTATGAAGCTGCAGTTTCAACAAAATTATTTGATGATGTTTTTGTTGTAACCGATTCTGATT
TGATTTTTGATGAAATTGTTTCTAATGGTGGAAAAGCCATTATGAGCATCAAAGAACACGAATCTGGAAGTGATCGAATT
GCAGAGGCTGTTGCAAATCTTGATGTTGATATTGTGGTAAATGTACAAGGAGACGAGCCTTTTACAGAAGCGGGGCCTTT
AGAACAGGTTTTATCTGTTTTTAAAAATGATCCAGATAAAAAGATTGATTTGGCTTCATTAATGCGTGAAATTACAAACG
AAGACGAAATCAACAATCCAAATAATGTGAAGGTTGTGGTAGATCAATCGCAGTTTGCGTTGTATTTTTCAAGATCAGTA
ATTCCGTATCCAAGAGAGAAAGATGCCGGAGTTCGTTATTTTCAGCATATCGGAATTTACGCTTTTAGGAAACAGGCTTT
GTTAGACTTTTACAGTCTTCCAATGAAATCTTTAGAAGCTTCAGAGAAATTAGAACAGCTGCGTTATTTAGAGTTTGGAA
AACGTATTAAAATGGTTGAAACAACTCATGTTGGAATTGGAATTGATACTGCTGAGGATTTAGAAAGAGCCAGAGCGATG
CTTAGAGATATTTAA

Upstream 100 bases:

>100_bases
TTTTAAAATACTGCCATTCAGTTTTATAACGATCTGTTTCGATTATGTTTTTTATCTAAGTTTTGAATACTTACATTTGT
TTTGCTAAAATAGATTTAAA

Downstream 100 bases:

>100_bases
CTCGTTATTGCGAGGAACGAAGCAATCTCACGACGTTTATCCTGTTAGTGTGATTGCTTGGTTCCTCGCAATGACCTTGA
CTTTGTATGATAAAAAAAGC

Product: 3-deoxy-D-manno-octulosonate cytidylyltransferase

Products: NA

Alternate protein names: CMP-2-keto-3-deoxyoctulosonic acid synthase; CKS; CMP-KDO synthase

Number of amino acids: Translated: 244; Mature: 244

Protein sequence:

>244_residues
MKIIAVIPARYASTRFPAKLMQDLGGKTVILRTYEAAVSTKLFDDVFVVTDSDLIFDEIVSNGGKAIMSIKEHESGSDRI
AEAVANLDVDIVVNVQGDEPFTEAGPLEQVLSVFKNDPDKKIDLASLMREITNEDEINNPNNVKVVVDQSQFALYFSRSV
IPYPREKDAGVRYFQHIGIYAFRKQALLDFYSLPMKSLEASEKLEQLRYLEFGKRIKMVETTHVGIGIDTAEDLERARAM
LRDI

Sequences:

>Translated_244_residues
MKIIAVIPARYASTRFPAKLMQDLGGKTVILRTYEAAVSTKLFDDVFVVTDSDLIFDEIVSNGGKAIMSIKEHESGSDRI
AEAVANLDVDIVVNVQGDEPFTEAGPLEQVLSVFKNDPDKKIDLASLMREITNEDEINNPNNVKVVVDQSQFALYFSRSV
IPYPREKDAGVRYFQHIGIYAFRKQALLDFYSLPMKSLEASEKLEQLRYLEFGKRIKMVETTHVGIGIDTAEDLERARAM
LRDI
>Mature_244_residues
MKIIAVIPARYASTRFPAKLMQDLGGKTVILRTYEAAVSTKLFDDVFVVTDSDLIFDEIVSNGGKAIMSIKEHESGSDRI
AEAVANLDVDIVVNVQGDEPFTEAGPLEQVLSVFKNDPDKKIDLASLMREITNEDEINNPNNVKVVVDQSQFALYFSRSV
IPYPREKDAGVRYFQHIGIYAFRKQALLDFYSLPMKSLEASEKLEQLRYLEFGKRIKMVETTHVGIGIDTAEDLERARAM
LRDI

Specific function: Activates KDO (a required 8-carbon sugar) for incorporation into bacterial lipopolysaccharide in Gram-negative bacteria

COG id: COG1212

COG function: function code M; CMP-2-keto-3-deoxyoctulosonic acid synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the kdsB family

Homologues:

Organism=Escherichia coli, GI1787147, Length=252, Percent_Identity=38.4920634920635, Blast_Score=169, Evalue=2e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): KDSB_FLAJ1 (A5FBA3)

Other databases:

- EMBL:   CP000685
- RefSeq:   YP_001196831.1
- ProteinModelPortal:   A5FBA3
- STRING:   A5FBA3
- GeneID:   5092586
- GenomeReviews:   CP000685_GR
- KEGG:   fjo:Fjoh_4513
- eggNOG:   COG1212
- HOGENOM:   HBG637773
- OMA:   IIPARLK
- ProtClustDB:   CLSK851559
- BioCyc:   FJOH376686:FJOH_4513-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00057
- InterPro:   IPR003329
- InterPro:   IPR004528
- TIGRFAMs:   TIGR00466

Pfam domain/function: PF02348 CTP_transf_3

EC number: =2.7.7.38

Molecular weight: Translated: 27469; Mature: 27469

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIIAVIPARYASTRFPAKLMQDLGGKTVILRTYEAAVSTKLFDDVFVVTDSDLIFDEIV
CEEEEEECCHHCCCCCCHHHHHHCCCCEEEEEEHHHHHHHHHHHCEEEEECCHHHHHHHH
SNGGKAIMSIKEHESGSDRIAEAVANLDVDIVVNVQGDEPFTEAGPLEQVLSVFKNDPDK
CCCCCEEEEECCCCCCHHHHHHHHHCCCEEEEEEECCCCCCCCCCCHHHHHHHHCCCCCC
KIDLASLMREITNEDEINNPNNVKVVVDQSQFALYFSRSVIPYPREKDAGVRYFQHIGIY
CCHHHHHHHHHCCCCCCCCCCCEEEEEECCCEEEEEECCCCCCCCCCCCHHHHHHHHHHH
AFRKQALLDFYSLPMKSLEASEKLEQLRYLEFGKRIKMVETTHVGIGIDTAEDLERARAM
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCEEEEEEEEEEEECCCCHHHHHHHHHH
LRDI
HHCC
>Mature Secondary Structure
MKIIAVIPARYASTRFPAKLMQDLGGKTVILRTYEAAVSTKLFDDVFVVTDSDLIFDEIV
CEEEEEECCHHCCCCCCHHHHHHCCCCEEEEEEHHHHHHHHHHHCEEEEECCHHHHHHHH
SNGGKAIMSIKEHESGSDRIAEAVANLDVDIVVNVQGDEPFTEAGPLEQVLSVFKNDPDK
CCCCCEEEEECCCCCCHHHHHHHHHCCCEEEEEEECCCCCCCCCCCHHHHHHHHCCCCCC
KIDLASLMREITNEDEINNPNNVKVVVDQSQFALYFSRSVIPYPREKDAGVRYFQHIGIY
CCHHHHHHHHHCCCCCCCCCCCEEEEEECCCEEEEEECCCCCCCCCCCCHHHHHHHHHHH
AFRKQALLDFYSLPMKSLEASEKLEQLRYLEFGKRIKMVETTHVGIGIDTAEDLERARAM
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCEEEEEEEEEEEECCCCHHHHHHHHHH
LRDI
HHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA