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The map label for this gene is htrB [H]

Identifier: 146302230

GI number: 146302230

Start: 5435976

End: 5436851

Strand: Direct

Name: htrB [H]

Synonym: Fjoh_4503

Alternate gene names: 146302230

Gene position: 5435976-5436851 (Clockwise)

Preceding gene: 146302223

Following gene: 146302232

Centisome position: 89.16

GC content: 33.9

Gene sequence:

>876_bases
ATGCAATTTCTCGTTTATATATTAGCCTACCCATTATTATGGCTGATCTCTATACTTCCATTCCGCTTATTTTATTTATT
CTCTGACTTTGTTTACTTTTTAGTTTACAGAGTAATTGGGTATCGCAAAAAAGTTGTACGCGAAAATCTGGCTCTTACTT
TACCGCATTTAAGTGATGCTGAAAGAAAAACAATCGAAAAGAAATTTTACCACCACATGTGCGATATGTTTTTGGAAATG
GTAAAAACGATGAGTATTTCGCCGGAAGAAATGGAAAAAAGATTCCATGTAACGAATATCGAATTGGTACAGGATTATTA
TAAAAAAGGCAAAAGCGTTATTCTTGTAGCCTCTCACTATGCAAGTTATGAGTGGCTTTTAACCATAAATCCAAAATTAG
GTTTTCAGGGCGTTGCGGTTTATAAAAGATTAGCCAATCGTTATTTTGATAAACTGATTAGAAAAATACGATCAAAATAC
AATACAGAAATGATCGAAACCAGACAAGCAATTCCAACAATGGCACAAAACCAGCGCAAAGGATTTGTAAGCATGTACGG
TTTAGCAAGTGATCAATCGCCAAAACTGGACCGAATTTTTCATTCGATGAAATTTATGGGTATTGAAGTTCCTGTACATA
CAGGCGCCGAAATGCTGGCCAAAAAATACGATCTAAGCGTAATTATGGTAAAAGTTAAAAAAGTAGCCAGAGGTTATTAT
GAAGCAACTTTTCTAACCATTGCAGATGATCCTAAAGAATATGAAAATTTCGACATCACTGAAAAGTATTTAAAAGAAGT
TGAAAAACAAATTTATGAAGCGCCAGAATATTATCTGTGGACTCATAAAAGATGGAAACATCGAGTAGAATCATAA

Upstream 100 bases:

>100_bases
ATGTGAAATGTAAATATAGTCAATATCGAACATATCTTGATGAGAATCCAAAATATCGATCATTTCTCATTTTTGCTTTA
TATTTGTAAAAAAAATATAC

Downstream 100 bases:

>100_bases
AAAAACACCTCTTTCAAAAAATATGAAAGAGGTGTTTTTTTTACGTATTGCAATCTTAAAATTAAATTCCTGCAATAACT
TTTATTTCATCAATAATTCG

Product: lipid A biosynthesis acyltransferase

Products: KDO2-(lauroyl)-lipid IVA [C]

Alternate protein names: Heat shock protein B [H]

Number of amino acids: Translated: 291; Mature: 291

Protein sequence:

>291_residues
MQFLVYILAYPLLWLISILPFRLFYLFSDFVYFLVYRVIGYRKKVVRENLALTLPHLSDAERKTIEKKFYHHMCDMFLEM
VKTMSISPEEMEKRFHVTNIELVQDYYKKGKSVILVASHYASYEWLLTINPKLGFQGVAVYKRLANRYFDKLIRKIRSKY
NTEMIETRQAIPTMAQNQRKGFVSMYGLASDQSPKLDRIFHSMKFMGIEVPVHTGAEMLAKKYDLSVIMVKVKKVARGYY
EATFLTIADDPKEYENFDITEKYLKEVEKQIYEAPEYYLWTHKRWKHRVES

Sequences:

>Translated_291_residues
MQFLVYILAYPLLWLISILPFRLFYLFSDFVYFLVYRVIGYRKKVVRENLALTLPHLSDAERKTIEKKFYHHMCDMFLEM
VKTMSISPEEMEKRFHVTNIELVQDYYKKGKSVILVASHYASYEWLLTINPKLGFQGVAVYKRLANRYFDKLIRKIRSKY
NTEMIETRQAIPTMAQNQRKGFVSMYGLASDQSPKLDRIFHSMKFMGIEVPVHTGAEMLAKKYDLSVIMVKVKKVARGYY
EATFLTIADDPKEYENFDITEKYLKEVEKQIYEAPEYYLWTHKRWKHRVES
>Mature_291_residues
MQFLVYILAYPLLWLISILPFRLFYLFSDFVYFLVYRVIGYRKKVVRENLALTLPHLSDAERKTIEKKFYHHMCDMFLEM
VKTMSISPEEMEKRFHVTNIELVQDYYKKGKSVILVASHYASYEWLLTINPKLGFQGVAVYKRLANRYFDKLIRKIRSKY
NTEMIETRQAIPTMAQNQRKGFVSMYGLASDQSPKLDRIFHSMKFMGIEVPVHTGAEMLAKKYDLSVIMVKVKKVARGYY
EATFLTIADDPKEYENFDITEKYLKEVEKQIYEAPEYYLWTHKRWKHRVES

Specific function: Acylates the intermediate (KDO)2-lipid IVA to form (KDO)2-(lauroyl)-lipid IVA. Has 10 fold selectivity for lauroyl- ACP over myristoyl-ACP [H]

COG id: COG1560

COG function: function code M; Lauroyl/myristoyl acyltransferase

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the htrB/msbB family [H]

Homologues:

Organism=Escherichia coli, GI1787292, Length=288, Percent_Identity=26.3888888888889, Blast_Score=85, Evalue=5e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004960
- InterPro:   IPR011920 [H]

Pfam domain/function: PF03279 Lip_A_acyltrans [H]

EC number: 2.3.1.- [C]

Molecular weight: Translated: 34802; Mature: 34802

Theoretical pI: Translated: 9.79; Mature: 9.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQFLVYILAYPLLWLISILPFRLFYLFSDFVYFLVYRVIGYRKKVVRENLALTLPHLSDA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCH
ERKTIEKKFYHHMCDMFLEMVKTMSISPEEMEKRFHVTNIELVQDYYKKGKSVILVASHY
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCHHHHHHHHHHHHCCCEEEEEEECC
ASYEWLLTINPKLGFQGVAVYKRLANRYFDKLIRKIRSKYNTEMIETRQAIPTMAQNQRK
CCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCC
GFVSMYGLASDQSPKLDRIFHSMKFMGIEVPVHTGAEMLAKKYDLSVIMVKVKKVARGYY
CHHHEEECCCCCCCHHHHHHHHHHHHEEECCHHCCHHHHHHHHCHHHHHHHHHHHHCCHH
EATFLTIADDPKEYENFDITEKYLKEVEKQIYEAPEYYLWTHKRWKHRVES
HHEEEEECCCCHHHCCCCHHHHHHHHHHHHHHHCCCCEEECCHHHHHCCCC
>Mature Secondary Structure
MQFLVYILAYPLLWLISILPFRLFYLFSDFVYFLVYRVIGYRKKVVRENLALTLPHLSDA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCH
ERKTIEKKFYHHMCDMFLEMVKTMSISPEEMEKRFHVTNIELVQDYYKKGKSVILVASHY
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCHHHHHHHHHHHHCCCEEEEEEECC
ASYEWLLTINPKLGFQGVAVYKRLANRYFDKLIRKIRSKYNTEMIETRQAIPTMAQNQRK
CCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCC
GFVSMYGLASDQSPKLDRIFHSMKFMGIEVPVHTGAEMLAKKYDLSVIMVKVKKVARGYY
CHHHEEECCCCCCCHHHHHHHHHHHHEEECCHHCCHHHHHHHHCHHHHHHHHHHHHCCHH
EATFLTIADDPKEYENFDITEKYLKEVEKQIYEAPEYYLWTHKRWKHRVES
HHEEEEECCCCHHHCCCCHHHHHHHHHHHHHHHCCCCEEECCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: KDO2-lipid IVA; lauroyl-ACP [C]

Specific reaction: KDO2-lipid IVA + lauroyl-ACP = KDO2-(lauroyl)-lipid IVA + [acyl carrier protein] [C]

General reaction: Acyltransferases [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]