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The map label for this gene is gcvH [H]

Identifier: 146302183

GI number: 146302183

Start: 5371154

End: 5371525

Strand: Direct

Name: gcvH [H]

Synonym: Fjoh_4452

Alternate gene names: 146302183

Gene position: 5371154-5371525 (Clockwise)

Preceding gene: 146302182

Following gene: 146302184

Centisome position: 88.1

GC content: 32.26

Gene sequence:

>372_bases
ATGTTTATTCCTAAATCGCTATATTATACTAAAAATCACTTGTGGTTAAGACAAGTTGGATTATACGATTATTTTGTCGG
TATTACTGATTTTGCCCAAAAAGAAATTGGAGAAATCAGTTTAATTGAACTCGAATTTACCGGTGAACACATGAAAAAAG
AAATGCAATGGGGAAAAATCTATGGACTTAATCAAACTTTTTCCCTCCTTGCCCCTATTGATTGCAAGGTTATGGCCACG
AACGTCATTTTGGATACCAACCCTTCAAGTATCAATCGAGATCCATACAATAATTGGTTCTTAAGAGTTTCAACAGTTGT
CAGTAATTATGGATTATTAACTAATGAAGAATATATAGAACTTATACAATGA

Upstream 100 bases:

>100_bases
AATCAAATATTGGCAGTGAGATTTTTATCAATATCAGAAACAAACCAGTTAAGGCAATTATTGTTCAGACTCCTTTTGTT
CAAATCTAAATCTTCGAGAT

Downstream 100 bases:

>100_bases
TATTTAAATTCCTTAAATTAAAAATAGCTGTTGAGGATAGCACCTTTAATGAACTATATCCAACCAGGATAAAAAGACTT
GCAGCCCGTCATTGGACTCC

Product: glycine cleavage H-protein

Products: Proton; NADH; NH3; CO2; 5,10-methylene-THF [C]

Alternate protein names: NA

Number of amino acids: Translated: 123; Mature: 123

Protein sequence:

>123_residues
MFIPKSLYYTKNHLWLRQVGLYDYFVGITDFAQKEIGEISLIELEFTGEHMKKEMQWGKIYGLNQTFSLLAPIDCKVMAT
NVILDTNPSSINRDPYNNWFLRVSTVVSNYGLLTNEEYIELIQ

Sequences:

>Translated_123_residues
MFIPKSLYYTKNHLWLRQVGLYDYFVGITDFAQKEIGEISLIELEFTGEHMKKEMQWGKIYGLNQTFSLLAPIDCKVMAT
NVILDTNPSSINRDPYNNWFLRVSTVVSNYGLLTNEEYIELIQ
>Mature_123_residues
MFIPKSLYYTKNHLWLRQVGLYDYFVGITDFAQKEIGEISLIELEFTGEHMKKEMQWGKIYGLNQTFSLLAPIDCKVMAT
NVILDTNPSSINRDPYNNWFLRVSTVVSNYGLLTNEEYIELIQ

Specific function: The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein [H]

COG id: COG0509

COG function: function code E; Glycine cleavage system H protein (lipoate-binding)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Caenorhabditis elegans, GI17551294, Length=123, Percent_Identity=31.7073170731707, Blast_Score=62, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR002930
- InterPro:   IPR017453
- InterPro:   IPR011053 [H]

Pfam domain/function: PF01597 GCV_H [H]

EC number: NA

Molecular weight: Translated: 14381; Mature: 14381

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFIPKSLYYTKNHLWLRQVGLYDYFVGITDFAQKEIGEISLIELEFTGEHMKKEMQWGKI
CCCCCCCEEECCCEEEEHHCHHHHHHHHHHHHHHCCCCEEEEEEEECCHHHHHHHCCCEE
YGLNQTFSLLAPIDCKVMATNVILDTNPSSINRDPYNNWFLRVSTVVSNYGLLTNEEYIE
EECCCCEEEECCCCEEEEEEEEEEECCCCCCCCCCCCCCEEEEEEHHHCCCCCCCHHHHH
LIQ
CCC
>Mature Secondary Structure
MFIPKSLYYTKNHLWLRQVGLYDYFVGITDFAQKEIGEISLIELEFTGEHMKKEMQWGKI
CCCCCCCEEECCCEEEEHHCHHHHHHHHHHHHHHCCCCEEEEEEEECCHHHHHHHCCCEE
YGLNQTFSLLAPIDCKVMATNVILDTNPSSINRDPYNNWFLRVSTVVSNYGLLTNEEYIE
EECCCCEEEECCCCEEEEEEEEEEECCCCCCCCCCCCCCEEEEEEHHHCCCCCCCHHHHH
LIQ
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Lipoyl Cofactor. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD; L-glycine; THF [C]

Specific reaction: NAD + L-glycine + THF = Proton + NADH + NH3 + CO2 + 5,10-methylene-THF [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA