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The map label for this gene is pehX [H]

Identifier: 146301992

GI number: 146301992

Start: 5140119

End: 5141546

Strand: Reverse

Name: pehX [H]

Synonym: Fjoh_4256

Alternate gene names: 146301992

Gene position: 5141546-5140119 (Counterclockwise)

Preceding gene: 146301994

Following gene: 146301991

Centisome position: 84.33

GC content: 33.89

Gene sequence:

>1428_bases
ATGATTACAAATAAGGTTATAGCGCTCAAATCAGTTGTTTTTTTAAGTATAACAGTCATTTTGACTATATCATGCTCAAA
AAAAATTACTCCTGAAGCTGAAACTGATCCGTGGAAAACTATGGAATTAACCATAAAAAACCTCCCAAAAACAAATTTTC
AGGATAAAACCTATAATATTAACGATTTTGGAGCTGTTGCAGACGGCAAAACTTTGAACACTGCGGCATTTGAAAAAGCT
ATTCAGACTTGTACTGAAAATGGGGGTGGAAAAGTTTTGGTTCCAAACGGAAAATACTTAACCGGAGCCATCCATTTAGA
AAACAATGTAAACCTTCATTTAGAAGATAAAGCTGAAATTTTGTTCAGTTTAAATCCAAAAGATTATCCAATCGTTCATA
CTTCATGGGAAGGAACTGAATTAATGAATTATTCACCGCTTATTTATGCTAAAAACAAAACCAATGTTGCCATAACAGGA
AAAGGAATTTTAAATGGTCAGGCTGATAGTACAAATTGGTGGATCTGGTCTGGAGCAAAAATGTACGGCTGGAAAAAAGG
AATTCCGTCTCAAAACGACCCGACAAATCGTGAAGTTTTGGTTGATATGGCAGAAAAAGACATTCCGGTTGAGCAGAGAA
TTTTTGGAGAAGGAAGATACCTGCGCCCAAATTTTATTGAGTTTTTTGAATGTAATACGGTTTTGGTAAAAGATATTACG
GTTATCAATTCTCCTTTTTGGATTTTACATCCAATAAAAACCAACAATATGATTATTGACGGTGTTACGGTAAACAGCCA
CGGACCAAATAATGACGGCTGTGACCCTGAGTATTCACAAAACATTGTAATTAAAAACTGCACATTCAATACCGGTGACG
ACTGTATTGCCATTAAAGCCGGACGTGATGCAGATGGAAGAAGAGTAGCTATTCCGAGTAAAAATATAATAGTACAAAAT
TGTAAAATGATCGACGGACACGGAGGTGTTGTTATTGGAAGTGAAATATCTGCCGGAGTAAACAATGTGTTTGTAGAAAA
TTGTGTAATGGACAGTCCAAATCTGGATCGTGCCATAAGAATTAAAACTAATTCAAGACGAGGCGGTATTATTGAAAATG
TATTTGTTAGAAACCTTGAAGTAGGAACAGTTAAAGAATGTGTATTAAAACTGAATATGTTTTATAACGTTTACGGGTCA
CAAACCGGAAACTTTATACCAGTAATAAGAAACATCAATCTTGAAAATGTAAACGTAAAAAATGGCGGAAAATACAGTAT
TTGGGCCGAAGGTTACAAAGAATCTCCTGTAGAAAATATTACGCTGAAAAACGTAAAAATCCAAAAAGTAGACTCGATCT
ATTTATTAAAGAATGTAAAAAATATCAATTTTATAAATACCTACATCAATGAAAAAAAAGTAGAATAA

Upstream 100 bases:

>100_bases
CTAAATAAATTTGGTTCGTTGAAACATATTTTCTATTTTCGTGTAATCGATTACATAATTATTGATCTTTTCACCTCGTT
AAACTAGAAATTCCACACAA

Downstream 100 bases:

>100_bases
ACTAAATACTAATGACTATTAACCAAAAAACCACTTAACAAAATTATGAACATTCAAAAATCATTAAAGAAAAAAATAAA
ATACAATCTTGTATTTTTAT

Product: glycoside hydrolase family protein

Products: NA

Alternate protein names: Exo-PG [H]

Number of amino acids: Translated: 475; Mature: 475

Protein sequence:

>475_residues
MITNKVIALKSVVFLSITVILTISCSKKITPEAETDPWKTMELTIKNLPKTNFQDKTYNINDFGAVADGKTLNTAAFEKA
IQTCTENGGGKVLVPNGKYLTGAIHLENNVNLHLEDKAEILFSLNPKDYPIVHTSWEGTELMNYSPLIYAKNKTNVAITG
KGILNGQADSTNWWIWSGAKMYGWKKGIPSQNDPTNREVLVDMAEKDIPVEQRIFGEGRYLRPNFIEFFECNTVLVKDIT
VINSPFWILHPIKTNNMIIDGVTVNSHGPNNDGCDPEYSQNIVIKNCTFNTGDDCIAIKAGRDADGRRVAIPSKNIIVQN
CKMIDGHGGVVIGSEISAGVNNVFVENCVMDSPNLDRAIRIKTNSRRGGIIENVFVRNLEVGTVKECVLKLNMFYNVYGS
QTGNFIPVIRNINLENVNVKNGGKYSIWAEGYKESPVENITLKNVKIQKVDSIYLLKNVKNINFINTYINEKKVE

Sequences:

>Translated_475_residues
MITNKVIALKSVVFLSITVILTISCSKKITPEAETDPWKTMELTIKNLPKTNFQDKTYNINDFGAVADGKTLNTAAFEKA
IQTCTENGGGKVLVPNGKYLTGAIHLENNVNLHLEDKAEILFSLNPKDYPIVHTSWEGTELMNYSPLIYAKNKTNVAITG
KGILNGQADSTNWWIWSGAKMYGWKKGIPSQNDPTNREVLVDMAEKDIPVEQRIFGEGRYLRPNFIEFFECNTVLVKDIT
VINSPFWILHPIKTNNMIIDGVTVNSHGPNNDGCDPEYSQNIVIKNCTFNTGDDCIAIKAGRDADGRRVAIPSKNIIVQN
CKMIDGHGGVVIGSEISAGVNNVFVENCVMDSPNLDRAIRIKTNSRRGGIIENVFVRNLEVGTVKECVLKLNMFYNVYGS
QTGNFIPVIRNINLENVNVKNGGKYSIWAEGYKESPVENITLKNVKIQKVDSIYLLKNVKNINFINTYINEKKVE
>Mature_475_residues
MITNKVIALKSVVFLSITVILTISCSKKITPEAETDPWKTMELTIKNLPKTNFQDKTYNINDFGAVADGKTLNTAAFEKA
IQTCTENGGGKVLVPNGKYLTGAIHLENNVNLHLEDKAEILFSLNPKDYPIVHTSWEGTELMNYSPLIYAKNKTNVAITG
KGILNGQADSTNWWIWSGAKMYGWKKGIPSQNDPTNREVLVDMAEKDIPVEQRIFGEGRYLRPNFIEFFECNTVLVKDIT
VINSPFWILHPIKTNNMIIDGVTVNSHGPNNDGCDPEYSQNIVIKNCTFNTGDDCIAIKAGRDADGRRVAIPSKNIIVQN
CKMIDGHGGVVIGSEISAGVNNVFVENCVMDSPNLDRAIRIKTNSRRGGIIENVFVRNLEVGTVKECVLKLNMFYNVYGS
QTGNFIPVIRNINLENVNVKNGGKYSIWAEGYKESPVENITLKNVKIQKVDSIYLLKNVKNINFINTYINEKKVE

Specific function: Contributes significantly to bacterial utilization of polygalacturonate and the induction of pectate lyase in the presence of extracellular pectic polymers [H]

COG id: COG5434

COG function: function code M; Endopolygalacturonase

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 4 PbH1 repeats [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008957
- InterPro:   IPR003961
- InterPro:   IPR000743
- InterPro:   IPR013783
- InterPro:   IPR006626
- InterPro:   IPR012334
- InterPro:   IPR011050 [H]

Pfam domain/function: PF00041 fn3; PF00295 Glyco_hydro_28 [H]

EC number: =3.2.1.82 [H]

Molecular weight: Translated: 52974; Mature: 52974

Theoretical pI: Translated: 8.12; Mature: 8.12

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS00502 POLYGALACTURONASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITNKVIALKSVVFLSITVILTISCSKKITPEAETDPWKTMELTIKNLPKTNFQDKTYNI
CCCCEEHHHHHHHHEEEEEEEEEECCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCEECC
NDFGAVADGKTLNTAAFEKAIQTCTENGGGKVLVPNGKYLTGAIHLENNVNLHLEDKAEI
CCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEECCCCEEEEEEEECCCCEEEEECCEEE
LFSLNPKDYPIVHTSWEGTELMNYSPLIYAKNKTNVAITGKGILNGQADSTNWWIWSGAK
EEEECCCCCCEEEECCCCCEEECCCCEEEECCCCCEEEEECEEECCCCCCCCEEEEECCE
MYGWKKGIPSQNDPTNREVLVDMAEKDIPVEQRIFGEGRYLRPNFIEFFECNTVLVKDIT
ECCEECCCCCCCCCCCCEEEEEECCCCCCHHHHHCCCCCEECCCCEEEEECCEEEEEEEE
VINSPFWILHPIKTNNMIIDGVTVNSHGPNNDGCDPEYSQNIVIKNCTFNTGDDCIAIKA
EECCCEEEEEEECCCCEEEEEEEECCCCCCCCCCCCCCCCCEEEEECCCCCCCCEEEEEE
GRDADGRRVAIPSKNIIVQNCKMIDGHGGVVIGSEISAGVNNVFVENCVMDSPNLDRAIR
CCCCCCCEEECCCCCEEEEEEEEEECCCCEEECCCCCCCCCCEEEEHEECCCCCCCEEEE
IKTNSRRGGIIENVFVRNLEVGTVKECVLKLNMFYNVYGSQTGNFIPVIRNINLENVNVK
EEECCCCCCEEEEEEEEECCCCHHHHHHHEEEEEEEEECCCCCCEEEEEEECCEEEEEEC
NGGKYSIWAEGYKESPVENITLKNVKIQKVDSIYLLKNVKNINFINTYINEKKVE
CCCEEEEEECCCCCCCCCEEEEEEEEEEEECEEEEEECCCCCEEEEEECCCCCCC
>Mature Secondary Structure
MITNKVIALKSVVFLSITVILTISCSKKITPEAETDPWKTMELTIKNLPKTNFQDKTYNI
CCCCEEHHHHHHHHEEEEEEEEEECCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCEECC
NDFGAVADGKTLNTAAFEKAIQTCTENGGGKVLVPNGKYLTGAIHLENNVNLHLEDKAEI
CCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEECCCCEEEEEEEECCCCEEEEECCEEE
LFSLNPKDYPIVHTSWEGTELMNYSPLIYAKNKTNVAITGKGILNGQADSTNWWIWSGAK
EEEECCCCCCEEEECCCCCEEECCCCEEEECCCCCEEEEECEEECCCCCCCCEEEEECCE
MYGWKKGIPSQNDPTNREVLVDMAEKDIPVEQRIFGEGRYLRPNFIEFFECNTVLVKDIT
ECCEECCCCCCCCCCCCEEEEEECCCCCCHHHHHCCCCCEECCCCEEEEECCEEEEEEEE
VINSPFWILHPIKTNNMIIDGVTVNSHGPNNDGCDPEYSQNIVIKNCTFNTGDDCIAIKA
EECCCEEEEEEECCCCEEEEEEEECCCCCCCCCCCCCCCCCEEEEECCCCCCCCEEEEEE
GRDADGRRVAIPSKNIIVQNCKMIDGHGGVVIGSEISAGVNNVFVENCVMDSPNLDRAIR
CCCCCCCEEECCCCCEEEEEEEEEECCCCEEECCCCCCCCCCEEEEHEECCCCCCCEEEE
IKTNSRRGGIIENVFVRNLEVGTVKECVLKLNMFYNVYGSQTGNFIPVIRNINLENVNVK
EEECCCCCCEEEEEEEEECCCCHHHHHHHEEEEEEEEECCCCCCEEEEEEECCEEEEEEC
NGGKYSIWAEGYKESPVENITLKNVKIQKVDSIYLLKNVKNINFINTYINEKKVE
CCCEEEEEECCCCCCCCCEEEEEEEEEEEECEEEEEECCCCCEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2168372; 1409594 [H]