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The map label for this gene is lutB [H]

Identifier: 146301947

GI number: 146301947

Start: 5060567

End: 5061970

Strand: Reverse

Name: lutB [H]

Synonym: Fjoh_4211

Alternate gene names: 146301947

Gene position: 5061970-5060567 (Counterclockwise)

Preceding gene: 146301948

Following gene: 146301946

Centisome position: 83.03

GC content: 40.24

Gene sequence:

>1404_bases
ATGTCATCAGAAAAAATAACACCGCACAGCGAAGCCGCAACAAAATTCAACAAAGATGTGGAACGTGTCAACTGGCATGA
CGAAACACTTTGGTTTGTTCGTGCGAAACGCGATAAAGCAGCTCATAATATTGATGACTGGGAGCTTCTTCGTGAAACGG
CTTCAAGAATAAAAGCCAATGTACTTTCAAATATTCACGATTATTTAGTAGAATTTGAGGCTAATGCCCAAAGAAACGGA
ATCATCGTACATTGGGCGGCCGATGCAAAAGAACACAACGAAATAGTGCATTCGATCATGGCAAAACATGATGTAAAGCA
AATGGTGAAATCAAAATCGATGCTTACAGAAGAATGCCATTTAAACGATTATTTAGCCGAAAAAGGCATAGAAGTTATCG
ACTCAGATTTAGGCGAATACATTGTACAGCTTCGTAAAGAACCGCCAAGTCATATTGTACTGCCTGCCATTCACCTTAAA
AAAGAAGATGTAAGTGAAACTTTTCACGAACATCTTGGAACCGAAAAAGGCAATTTTAACCCGCAGTACTTAACAGAATC
TGCCCGTCATAGTTTAAGAAATACTTTCCTGACCAGAAAAGCAGCTTTAACCGGAGTAAATTTTGCAGTGGCAGAAACCG
GAGAATTTGTGGTCTGCACCAACGAAGGAAATGCCGATATGGGCGCACATTTAGCCGATGTTCATATCGCCTGTATGGGA
TTTGAAAAACTCATTCCGCAGCGAAAACATCTAGGCGTTTTCCTTAGATTATTAGCAAGAAGTGCTACAGGACAGCCTAT
AACTACTTTTTCTAGTCATTTTAAGAAACCAAGAGACGGAAAAGAACTTCATATTGTAATTGTCGACAACGGCAGAAGCA
AACAGTTAGGACGCGAAGATTTTAGAAATTCACTAAAATGTATTCGCTGTGGCGCCTGCATGAATACATGCCCTGTTTAC
AGACGAAGCGGCGGGCATAGTTATCATAATGCGGTTGCGGGACCAATTGGTTCTATTCTGGCACCCAACTTAGATATGAG
CAAAAATGCCGATCTGCCTTTTGCCAGTACGCTTTGCGGTTCCTGTACCAATGTTTGTCCGGTAAAAATTGATATTCACG
ATCAATTGTACAAATGGCGTCAGGTTTTGGTAAAAGACGGCTATACGCCAAAAGCAAAAACCATAGCCATGAAAACGATG
GCAACAGTTTTGGCCAATCCAACGATTTTTAATATTGCAGGAAAATCAGGAAGATTTGTAATGAAAAATGTTCCGGCAAT
GGTAAATAATAAAATGAACAAATGGTACGACCAGCGCGAAATGCCAGACGTACCGCAGGAATCTTTTAGAGAATGGTACA
AGAAAAATTCGAGAGAATCTAAAACAAAAGACAATGAGCAGTAA

Upstream 100 bases:

>100_bases
TAATTTTGATTCTTGCGTACCTTGCGTTTTTTTCTTTGCGCACTTTGCGGTTAAATAAACCATTATAAACATTTCAAAAT
TGACTTTTAAATAATTAAAA

Downstream 100 bases:

>100_bases
AGCCGAAATTTTAAACAGAATAAAAGCGAATCAGCCTGATTTGGTTACGGAACTGCCGGATTTAAATCTTTTAGGTTCCG
AGAATTTTGATACACTCGAA

Product: 4Fe-4S ferredoxin iron-sulfur binding domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 467; Mature: 466

Protein sequence:

>467_residues
MSSEKITPHSEAATKFNKDVERVNWHDETLWFVRAKRDKAAHNIDDWELLRETASRIKANVLSNIHDYLVEFEANAQRNG
IIVHWAADAKEHNEIVHSIMAKHDVKQMVKSKSMLTEECHLNDYLAEKGIEVIDSDLGEYIVQLRKEPPSHIVLPAIHLK
KEDVSETFHEHLGTEKGNFNPQYLTESARHSLRNTFLTRKAALTGVNFAVAETGEFVVCTNEGNADMGAHLADVHIACMG
FEKLIPQRKHLGVFLRLLARSATGQPITTFSSHFKKPRDGKELHIVIVDNGRSKQLGREDFRNSLKCIRCGACMNTCPVY
RRSGGHSYHNAVAGPIGSILAPNLDMSKNADLPFASTLCGSCTNVCPVKIDIHDQLYKWRQVLVKDGYTPKAKTIAMKTM
ATVLANPTIFNIAGKSGRFVMKNVPAMVNNKMNKWYDQREMPDVPQESFREWYKKNSRESKTKDNEQ

Sequences:

>Translated_467_residues
MSSEKITPHSEAATKFNKDVERVNWHDETLWFVRAKRDKAAHNIDDWELLRETASRIKANVLSNIHDYLVEFEANAQRNG
IIVHWAADAKEHNEIVHSIMAKHDVKQMVKSKSMLTEECHLNDYLAEKGIEVIDSDLGEYIVQLRKEPPSHIVLPAIHLK
KEDVSETFHEHLGTEKGNFNPQYLTESARHSLRNTFLTRKAALTGVNFAVAETGEFVVCTNEGNADMGAHLADVHIACMG
FEKLIPQRKHLGVFLRLLARSATGQPITTFSSHFKKPRDGKELHIVIVDNGRSKQLGREDFRNSLKCIRCGACMNTCPVY
RRSGGHSYHNAVAGPIGSILAPNLDMSKNADLPFASTLCGSCTNVCPVKIDIHDQLYKWRQVLVKDGYTPKAKTIAMKTM
ATVLANPTIFNIAGKSGRFVMKNVPAMVNNKMNKWYDQREMPDVPQESFREWYKKNSRESKTKDNEQ
>Mature_466_residues
SSEKITPHSEAATKFNKDVERVNWHDETLWFVRAKRDKAAHNIDDWELLRETASRIKANVLSNIHDYLVEFEANAQRNGI
IVHWAADAKEHNEIVHSIMAKHDVKQMVKSKSMLTEECHLNDYLAEKGIEVIDSDLGEYIVQLRKEPPSHIVLPAIHLKK
EDVSETFHEHLGTEKGNFNPQYLTESARHSLRNTFLTRKAALTGVNFAVAETGEFVVCTNEGNADMGAHLADVHIACMGF
EKLIPQRKHLGVFLRLLARSATGQPITTFSSHFKKPRDGKELHIVIVDNGRSKQLGREDFRNSLKCIRCGACMNTCPVYR
RSGGHSYHNAVAGPIGSILAPNLDMSKNADLPFASTLCGSCTNVCPVKIDIHDQLYKWRQVLVKDGYTPKAKTIAMKTMA
TVLANPTIFNIAGKSGRFVMKNVPAMVNNKMNKWYDQREMPDVPQESFREWYKKNSRESKTKDNEQ

Specific function: Is involved in L-lactate degradation and allows cells to grow with lactate as the sole carbon source. Has probably a role as an electron transporter during oxidation of L-lactate [H]

COG id: COG1139

COG function: function code C; Uncharacterized conserved protein containing a ferredoxin-like domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1786498, Length=441, Percent_Identity=39.2290249433107, Blast_Score=342, Evalue=3e-95,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017900
- InterPro:   IPR003741
- InterPro:   IPR004452
- InterPro:   IPR002698
- InterPro:   IPR012285
- InterPro:   IPR009051
- InterPro:   IPR022825 [H]

Pfam domain/function: PF02589 DUF162 [H]

EC number: NA

Molecular weight: Translated: 52847; Mature: 52716

Theoretical pI: Translated: 8.67; Mature: 8.67

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSEKITPHSEAATKFNKDVERVNWHDETLWFVRAKRDKAAHNIDDWELLRETASRIKAN
CCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEECCHHCCCCCCHHHHHHHHHHHHHHH
VLSNIHDYLVEFEANAQRNGIIVHWAADAKEHNEIVHSIMAKHDVKQMVKSKSMLTEECH
HHHHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LNDYLAEKGIEVIDSDLGEYIVQLRKEPPSHIVLPAIHLKKEDVSETFHEHLGTEKGNFN
HHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCEEECCHHCCHHHHHHHHHHHHCCCCCCCC
PQYLTESARHSLRNTFLTRKAALTGVNFAVAETGEFVVCTNEGNADMGAHLADVHIACMG
HHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCEEEEECCCCCCCCCHHHHHHHHHHH
FEKLIPQRKHLGVFLRLLARSATGQPITTFSSHFKKPRDGKELHIVIVDNGRSKQLGRED
HHHHCCCHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCCCEEEEEEEECCCCCCCCHHH
FRNSLKCIRCGACMNTCPVYRRSGGHSYHNAVAGPIGSILAPNLDMSKNADLPFASTLCG
HHHHHHHHHHCCHHHCCCHHHCCCCCCHHHHHHCCHHHHHCCCCCCCCCCCCCHHHHHHC
SCTNVCPVKIDIHDQLYKWRQVLVKDGYTPKAKTIAMKTMATVLANPTIFNIAGKSGRFV
CCCCCCEEEEEHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCCH
MKNVPAMVNNKMNKWYDQREMPDVPQESFREWYKKNSRESKTKDNEQ
HHCCCHHHHCHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
SSEKITPHSEAATKFNKDVERVNWHDETLWFVRAKRDKAAHNIDDWELLRETASRIKAN
CCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEECCHHCCCCCCHHHHHHHHHHHHHHH
VLSNIHDYLVEFEANAQRNGIIVHWAADAKEHNEIVHSIMAKHDVKQMVKSKSMLTEECH
HHHHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LNDYLAEKGIEVIDSDLGEYIVQLRKEPPSHIVLPAIHLKKEDVSETFHEHLGTEKGNFN
HHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCEEECCHHCCHHHHHHHHHHHHCCCCCCCC
PQYLTESARHSLRNTFLTRKAALTGVNFAVAETGEFVVCTNEGNADMGAHLADVHIACMG
HHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCEEEEECCCCCCCCCHHHHHHHHHHH
FEKLIPQRKHLGVFLRLLARSATGQPITTFSSHFKKPRDGKELHIVIVDNGRSKQLGRED
HHHHCCCHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCCCEEEEEEEECCCCCCCCHHH
FRNSLKCIRCGACMNTCPVYRRSGGHSYHNAVAGPIGSILAPNLDMSKNADLPFASTLCG
HHHHHHHHHHCCHHHCCCHHHCCCCCCHHHHHHCCHHHHHCCCCCCCCCCCCCHHHHHHC
SCTNVCPVKIDIHDQLYKWRQVLVKDGYTPKAKTIAMKTMATVLANPTIFNIAGKSGRFV
CCCCCCEEEEEHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCCH
MKNVPAMVNNKMNKWYDQREMPDVPQESFREWYKKNSRESKTKDNEQ
HHCCCHHHHCHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA