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The map label for this gene is yieH [H]

Identifier: 146301920

GI number: 146301920

Start: 5025277

End: 5025915

Strand: Reverse

Name: yieH [H]

Synonym: Fjoh_4184

Alternate gene names: 146301920

Gene position: 5025915-5025277 (Counterclockwise)

Preceding gene: 146301921

Following gene: 146301914

Centisome position: 82.43

GC content: 36.31

Gene sequence:

>639_bases
ATGAATGTAAAATGTATTATTTTCGATTGTGACGGAGTTTTGGTTGATACTGAGAAAATTGGCAACGGAATACTGCTGGC
AATGGCTCAGGAACACGGATTCGAAATGGAACTCGAAGATGCATACCGATATTTTAACGGTCGAAATTTAAAAGATTGTT
TTCGTCATATTGAAGAAGCAATTGACCAGAAACTGCCTGAAAATTTTGAAACAGAATATCGCGAGAAAAGTTTTGAAGCT
TTTAAAACACAGGTAAAACCTATGAAAGGCATTGAAGATTTTATTGCAAAATTAAAAATTCCGTATTGTGTGGCATCAAG
CGGACCTGTCGAAAAAATCCGTTTGAATCTTGAAGTGTCGGGGTTGATCGACAAATTCGAAAATAAAATATTCAGTTCGT
ATCAAATTGGCAGCTGGAAACCAGAACCGGGAATTTTTCTGCAGGCCGCTCAGCAAATGGGTTTTGAAGTAAAAGACTGT
ATTGTGATAGAAGACAGTAAAGCAGGAGTAAAGGCAGGTGTAAGCGGAGGTTTTAAAGTATACGGTTTTGTTAATGGTTT
TAATAACGAAGATTTAGAAGAAGAAGGAGCCATTTTATTTGACAGCTACGAAGAATTGAGTAAAATGCTGTCAATTTAA

Upstream 100 bases:

>100_bases
CTTTAAAGCACAATAACGGCTTTGCAAAATAATTTTTGTACTTTCCATTTTGGTTTACCGCTAATAGTATAAAGCGGTTC
TAAAAAGTAAATTAAAAATT

Downstream 100 bases:

>100_bases
TTTTAGAAAATAATCTATAAAAAAAGCTGTCCTTTTTGAGGACAGCTTTACATTAGTTGTGTTGTAGTTTAATAATTAAT
TTATTTTAACCAAATGAGCT

Product: HAD family hydrolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 212; Mature: 212

Protein sequence:

>212_residues
MNVKCIIFDCDGVLVDTEKIGNGILLAMAQEHGFEMELEDAYRYFNGRNLKDCFRHIEEAIDQKLPENFETEYREKSFEA
FKTQVKPMKGIEDFIAKLKIPYCVASSGPVEKIRLNLEVSGLIDKFENKIFSSYQIGSWKPEPGIFLQAAQQMGFEVKDC
IVIEDSKAGVKAGVSGGFKVYGFVNGFNNEDLEEEGAILFDSYEELSKMLSI

Sequences:

>Translated_212_residues
MNVKCIIFDCDGVLVDTEKIGNGILLAMAQEHGFEMELEDAYRYFNGRNLKDCFRHIEEAIDQKLPENFETEYREKSFEA
FKTQVKPMKGIEDFIAKLKIPYCVASSGPVEKIRLNLEVSGLIDKFENKIFSSYQIGSWKPEPGIFLQAAQQMGFEVKDC
IVIEDSKAGVKAGVSGGFKVYGFVNGFNNEDLEEEGAILFDSYEELSKMLSI
>Mature_212_residues
MNVKCIIFDCDGVLVDTEKIGNGILLAMAQEHGFEMELEDAYRYFNGRNLKDCFRHIEEAIDQKLPENFETEYREKSFEA
FKTQVKPMKGIEDFIAKLKIPYCVASSGPVEKIRLNLEVSGLIDKFENKIFSSYQIGSWKPEPGIFLQAAQQMGFEVKDC
IVIEDSKAGVKAGVSGGFKVYGFVNGFNNEDLEEEGAILFDSYEELSKMLSI

Specific function: Catalyzes the dephosphorylation of the artificial chromogenic substrate p-nitrophenyl phosphate (pNPP) and of the natural substrates phosphoenolpyruvate and AMP [H]

COG id: COG0637

COG function: function code R; Predicted phosphatase/phosphohexomutase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. CbbY/CbbZ/Gph/YieH family [H]

Homologues:

Organism=Escherichia coli, GI1790151, Length=209, Percent_Identity=28.2296650717703, Blast_Score=117, Evalue=7e-28,
Organism=Escherichia coli, GI87082080, Length=212, Percent_Identity=25.4716981132075, Blast_Score=62, Evalue=3e-11,
Organism=Drosophila melanogaster, GI116008157, Length=186, Percent_Identity=29.5698924731183, Blast_Score=68, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006439
- InterPro:   IPR006402
- InterPro:   IPR005833 [H]

Pfam domain/function: PF00702 Hydrolase [H]

EC number: NA

Molecular weight: Translated: 23976; Mature: 23976

Theoretical pI: Translated: 4.42; Mature: 4.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVKCIIFDCDGVLVDTEKIGNGILLAMAQEHGFEMELEDAYRYFNGRNLKDCFRHIEEA
CCEEEEEEECCCEEEEHHHCCCEEEEEEECCCCCEEEHHHHHHHHCCCCHHHHHHHHHHH
IDQKLPENFETEYREKSFEAFKTQVKPMKGIEDFIAKLKIPYCVASSGPVEKIRLNLEVS
HHHHCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCEEECCCCCCEEEEEEEEHH
GLIDKFENKIFSSYQIGSWKPEPGIFLQAAQQMGFEVKDCIVIEDSKAGVKAGVSGGFKV
HHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCEEEEEEEEECCCCCCCCCCCCCEEE
YGFVNGFNNEDLEEEGAILFDSYEELSKMLSI
EEEEECCCCCCCCCCCCEEEECHHHHHHHHCC
>Mature Secondary Structure
MNVKCIIFDCDGVLVDTEKIGNGILLAMAQEHGFEMELEDAYRYFNGRNLKDCFRHIEEA
CCEEEEEEECCCEEEEHHHCCCEEEEEEECCCCCEEEHHHHHHHHCCCCHHHHHHHHHHH
IDQKLPENFETEYREKSFEAFKTQVKPMKGIEDFIAKLKIPYCVASSGPVEKIRLNLEVS
HHHHCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCEEECCCCCCEEEEEEEEHH
GLIDKFENKIFSSYQIGSWKPEPGIFLQAAQQMGFEVKDCIVIEDSKAGVKAGVSGGFKV
HHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCEEEEEEEEECCCCCCCCCCCCCEEE
YGFVNGFNNEDLEEEGAILFDSYEELSKMLSI
EEEEECCCCCCCCCCCCEEEECHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7686882; 9278503 [H]