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The map label for this gene is mexA [H]

Identifier: 146301868

GI number: 146301868

Start: 4967751

End: 4968905

Strand: Reverse

Name: mexA [H]

Synonym: Fjoh_4132

Alternate gene names: 146301868

Gene position: 4968905-4967751 (Counterclockwise)

Preceding gene: 146301875

Following gene: 146301867

Centisome position: 81.5

GC content: 36.8

Gene sequence:

>1155_bases
ATGAATAAGCAATCATTTTTAAGCATTCTCGCAGCATCAGTTATTATCGCATCATGCGGTAAAAATGATAAATCGGCTCA
GGCCGGAGGAGCACCGCAGGTTAAAGAGTATAAAACGCTGACTTTACAGCCGGAATCTGCTACATTAAACAGCGATTATC
CAGCCAGTATTCAAGGACAGCAGAATATAGAAATCCGACCAAGAGTGGAAGGTTACATCGATAAAATTTTTGTAGATGAA
GGTGCTGTTGTAAAAGCAGGACAGCCGTTATTCAAAATCAGCGCGCCAGAATATGAGCAGCAAGTTCGTACAGCAACAGC
AAGTATTAAAAGTGCTCAGGCTGACTTGAGTTCTGCAAAACTGGCTGTAAACAAAGTAAAACCTTTGGTTGAAAAAGGAA
TTATAAGTAAATACGATTTAGAATCGGCACAATATACTTATGAGTCTGCTTTAGCCTCTTTAGCTCAGGCAAACGCAGCT
TTGGTAAATGCTAAAACTAATTTAGGATATACTACGGTTACAAGTCCGGTTAATGGAGTTGTAGGTTCAATTCCGTTTCG
TTTAGGAAGTTTAGTAAGCTCAAATACAGCAGAACCTTTAACAACGGTTTCAAGTATTGGAAACGTATATGCTTATTTTG
CTTTAAATGAAAAAGCGCTTTTAAATTTTACTAAAGATTCTGGAGCTTCATTAAACCAAAAATTAAAAAGCATGCCGGCA
GTTTCTTTATTGCTTTCAGATGGTTCTGCTTATGATCAAAAAGGAAACATAGAAACCGTAAACGGATTAATCAATACTGA
AACAGGTACTGTAAACGTAAGAGCTCGTTTCCCAAATCCAAAAGGAATTATCAGAAGCGGAAGCAGTACTACAGTAAGAA
TTCCAAAAGAAGTAAAAGACGGAATCATTGTTCCTCAAAGTGCAACATTTGAACTTCAGGATAAAATGTTTGCAGTAGTT
TTAGGAAAAGACGGAAAAACAAGAAATGCTAATATCACAGTACTTGAAAATACAGCAGGAAACTATTACGTAGTAACAAG
CGGTTTAAAACCTGGAGACGAAATAGTTTTAGAAGGAGTAGCTTCTCTAAAAGAGGGATCTGAAATTAAAGCTAAAAAAC
AAAGCCCGGAAACAGTTTACGCCGATTTAAAATAA

Upstream 100 bases:

>100_bases
TTTATCGATTGTCTTTTGCTTTTCGTCTATTTGAAATTTTTTGTATGTTATATTTTATTTCTTTTGTTGTCATATAATCG
ATAATTACATATTATTTCTA

Downstream 100 bases:

>100_bases
AAGAAATGTTTAAAAGATTTATACAAAGACCCGTACTCTCGACGGTAATATCTGTTATTATCGTTATTTTAGGTGTTTTA
GGCCTAATAGAGTTACCGAT

Product: RND family efflux transporter MFP subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 384; Mature: 384

Protein sequence:

>384_residues
MNKQSFLSILAASVIIASCGKNDKSAQAGGAPQVKEYKTLTLQPESATLNSDYPASIQGQQNIEIRPRVEGYIDKIFVDE
GAVVKAGQPLFKISAPEYEQQVRTATASIKSAQADLSSAKLAVNKVKPLVEKGIISKYDLESAQYTYESALASLAQANAA
LVNAKTNLGYTTVTSPVNGVVGSIPFRLGSLVSSNTAEPLTTVSSIGNVYAYFALNEKALLNFTKDSGASLNQKLKSMPA
VSLLLSDGSAYDQKGNIETVNGLINTETGTVNVRARFPNPKGIIRSGSSTTVRIPKEVKDGIIVPQSATFELQDKMFAVV
LGKDGKTRNANITVLENTAGNYYVVTSGLKPGDEIVLEGVASLKEGSEIKAKKQSPETVYADLK

Sequences:

>Translated_384_residues
MNKQSFLSILAASVIIASCGKNDKSAQAGGAPQVKEYKTLTLQPESATLNSDYPASIQGQQNIEIRPRVEGYIDKIFVDE
GAVVKAGQPLFKISAPEYEQQVRTATASIKSAQADLSSAKLAVNKVKPLVEKGIISKYDLESAQYTYESALASLAQANAA
LVNAKTNLGYTTVTSPVNGVVGSIPFRLGSLVSSNTAEPLTTVSSIGNVYAYFALNEKALLNFTKDSGASLNQKLKSMPA
VSLLLSDGSAYDQKGNIETVNGLINTETGTVNVRARFPNPKGIIRSGSSTTVRIPKEVKDGIIVPQSATFELQDKMFAVV
LGKDGKTRNANITVLENTAGNYYVVTSGLKPGDEIVLEGVASLKEGSEIKAKKQSPETVYADLK
>Mature_384_residues
MNKQSFLSILAASVIIASCGKNDKSAQAGGAPQVKEYKTLTLQPESATLNSDYPASIQGQQNIEIRPRVEGYIDKIFVDE
GAVVKAGQPLFKISAPEYEQQVRTATASIKSAQADLSSAKLAVNKVKPLVEKGIISKYDLESAQYTYESALASLAQANAA
LVNAKTNLGYTTVTSPVNGVVGSIPFRLGSLVSSNTAEPLTTVSSIGNVYAYFALNEKALLNFTKDSGASLNQKLKSMPA
VSLLLSDGSAYDQKGNIETVNGLINTETGTVNVRARFPNPKGIIRSGSSTTVRIPKEVKDGIIVPQSATFELQDKMFAVV
LGKDGKTRNANITVLENTAGNYYVVTSGLKPGDEIVLEGVASLKEGSEIKAKKQSPETVYADLK

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Lipid-anchor. Note=The membrane anchor is not necessary for antibiotic efflux as the protein is functional when targeted to the periplasm by a foreign signal peptide [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1789929, Length=381, Percent_Identity=28.8713910761155, Blast_Score=152, Evalue=5e-38,
Organism=Escherichia coli, GI1786668, Length=379, Percent_Identity=29.5514511873351, Blast_Score=140, Evalue=2e-34,
Organism=Escherichia coli, GI1789665, Length=373, Percent_Identity=26.8096514745308, Blast_Score=131, Evalue=7e-32,
Organism=Escherichia coli, GI87082036, Length=310, Percent_Identity=21.2903225806452, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI87081796, Length=343, Percent_Identity=23.9067055393586, Blast_Score=70, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 40765; Mature: 40765

Theoretical pI: Translated: 9.36; Mature: 9.36

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKQSFLSILAASVIIASCGKNDKSAQAGGAPQVKEYKTLTLQPESATLNSDYPASIQGQ
CCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCC
QNIEIRPRVEGYIDKIFVDEGAVVKAGQPLFKISAPEYEQQVRTATASIKSAQADLSSAK
CCCEECCCHHCEEEEEEECCCCEEECCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHH
LAVNKVKPLVEKGIISKYDLESAQYTYESALASLAQANAALVNAKTNLGYTTVTSPVNGV
HHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCEEEEEECCCCCEEEECCCCCCH
VGSIPFRLGSLVSSNTAEPLTTVSSIGNVYAYFALNEKALLNFTKDSGASLNQKLKSMPA
HHCCCHHHHHHHCCCCCCHHHHHHHHCCEEEEEEECCCEEEEECCCCCCHHHHHHHCCCE
VSLLLSDGSAYDQKGNIETVNGLINTETGTVNVRARFPNPKGIIRSGSSTTVRIPKEVKD
EEEEEECCCCCCCCCCEEEECCEEECCCCEEEEEEECCCCCCEEECCCCCEEEECHHHHC
GIIVPQSATFELQDKMFAVVLGKDGKTRNANITVLENTAGNYYVVTSGLKPGDEIVLEGV
CEECCCCCCEEECCCEEEEEECCCCCCCCCEEEEEECCCCCEEEEECCCCCCHHHHHHHH
ASLKEGSEIKAKKQSPETVYADLK
HHHCCCCCCEECCCCCCEEEEECC
>Mature Secondary Structure
MNKQSFLSILAASVIIASCGKNDKSAQAGGAPQVKEYKTLTLQPESATLNSDYPASIQGQ
CCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCC
QNIEIRPRVEGYIDKIFVDEGAVVKAGQPLFKISAPEYEQQVRTATASIKSAQADLSSAK
CCCEECCCHHCEEEEEEECCCCEEECCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHH
LAVNKVKPLVEKGIISKYDLESAQYTYESALASLAQANAALVNAKTNLGYTTVTSPVNGV
HHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCEEEEEECCCCCEEEECCCCCCH
VGSIPFRLGSLVSSNTAEPLTTVSSIGNVYAYFALNEKALLNFTKDSGASLNQKLKSMPA
HHCCCHHHHHHHCCCCCCHHHHHHHHCCEEEEEEECCCEEEEECCCCCCHHHHHHHCCCE
VSLLLSDGSAYDQKGNIETVNGLINTETGTVNVRARFPNPKGIIRSGSSTTVRIPKEVKD
EEEEEECCCCCCCCCCEEEECCEEECCCCEEEEEEECCCCCCEEECCCCCEEEECHHHHC
GIIVPQSATFELQDKMFAVVLGKDGKTRNANITVLENTAGNYYVVTSGLKPGDEIVLEGV
CEECCCCCCEEECCCEEEEEECCCCCCCCCEEEEEECCCCCEEEEECCCCCCHHHHHHHH
ASLKEGSEIKAKKQSPETVYADLK
HHHCCCCCCEECCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7968531; 10984043; 9989496; 8226684; 8540696; 9603892; 10671490 [H]