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The map label for this gene is pgl [H]

Identifier: 146301851

GI number: 146301851

Start: 4945891

End: 4947573

Strand: Direct

Name: pgl [H]

Synonym: Fjoh_4115

Alternate gene names: 146301851

Gene position: 4945891-4947573 (Clockwise)

Preceding gene: 146301845

Following gene: 146301852

Centisome position: 81.12

GC content: 35.59

Gene sequence:

>1683_bases
ATGAAAACTAACCTAACTAACCTTTTCTGCACAGCACTGACCATTCTGGCAGTAAGCTTTTCTGTACCGGTTTTGGCACA
AAAAGCGTCTGATACTTACAAAGACATTGAATTTAAAATGGCCAAAATCAAAGAGCCGGTTATTCCTAAAAACAGTGTAA
ACATAAAAGATTTTGGTGCGGTTAACGGTGGTTATGTCTTAAACACGAAGGCTTTTGCTGATGCTATTGATGCTGTTTCT
AAAAAGGGCGGCGGAAAAGTTATTATTCCTCCGGGAATCTGGCTTACAGGTCCAATTATTCTAAAAAGCAATATCGAATT
ACACGCTGAAAGAGGCGCTTTAATAAAATTTTCTACAGACAAATCATTATATCCTATTATCGGAACTAATTTTGAAGGAC
TAAATACCTGGCGATGCATCTCTCCTATTTATGGTAAAAATCTTGAGAACATTGCTTTTACCGGAAATGGTGTTTGGGAT
GGTTCTGGAGAAGTTTGGAGACAGGTTAAAAAAAGTAAGTTGACAGAAAGCCAGTGGAAAAAATTTGTCAGTTCAGGCGG
GGTTTTAAATAAAGACAAAACAAGCTGGTATCCATCTGAAACTTTTATGAACGCTTCTAAAGGTGCTGATCAAAACGTAC
GTCCGGATTTAAAAACGAAAGAAGAATTTGAAACTATTCACGATTTTCTGCGTCCGGTAATGGTAAGTATTCAAAACAGC
AAAAGAGTTTTATTTGACGGTCCAGTTTTTCAAAATTCACCAGCTTGGAATATTCACCCTTTTATGGTAGAAGATTTAAT
TGTGAGAAATGTAACGGTTCGTAATCCGTGGTATTCTCAAAATGGTGACGGTCTTGATGTAGAATGCTGTAAAAATGTTT
TGGTTGAAAATTCAAGTTTTGATGTGGGTGACGATGCCATCTGTATTAAATCCGGAAAAGATAAGGATGGACTTGAAAGA
GGTATTCCGTGCGAAAATATTATTGTGAGAAACAATATCGTATATCACGGACACGGCGGCGTAACGGTTGGAAGTGAAAT
GTCAGGAGGTGTAAAAAACCTGCATGTTTCAAATTGTACTTTTATGGGAACTGATGTTGGTCTGCGTTTTAAAAGTGCAC
GCGGCAGAGGCGGTGTTGTAGAAAATATCTTTATTTCAGATGTTTTTATGACTGATATTCCATCGCAGGCTATTTCTTTT
AACTTGTATTATGGAGGAAAATCTATTGCTGAAACTTTAGAAGAAGGCGGAGACAAAATCGTTAACAAAGCAATGCCTGT
AACGATTGAAACGCCTCAGTTTAAAAACATTTCAATCAAAAACATTACGATCAAAGGTGCACAGCAGGCAGTATTTTTAC
AAGGTCTTCCTGAAATGAATCTTGAAAATATCGAAATTTCAAACTTGACAGTTACGGCTGAAAAAGGATTTTCTATTATT
GACGCAAAAGGAATTAAAATTACCAATGCGAAACTGGATATCGAAAACAGCAATGTATTTGAAGTTTACAATGTAAAAAA
TATGTCTTTAAAAGATGTTGAGTTTAATTCTACATCTGCTAAAGCTGTAAATATAAATGGTGAAGGAAACGCAAATATAG
AACTGGTTTCTTCTAAAAAATTAGATTTTTCAAAAACGACTACTCTTGGAAATGATGTTCCAAAAGGAGCTGTAAAATTT
TAA

Upstream 100 bases:

>100_bases
GAGAATATTTTTATTCATGAATCAAAAAATTAACTAATAAAAAATCGACTCAGCATCAAAAAATACTCAAAAAGTAAATT
TTAACCATAATACAATACGA

Downstream 100 bases:

>100_bases
TCACAATTTCAATATGAAATTTATCAATAGTTCAAAAGCAGTTGTTGTAAGTCTTTGTGCTTTTTCGTTTCTACATTCAA
ATGCGCAGACTAACAAAGAG

Product: glycoside hydrolase family protein

Products: NA

Alternate protein names: PGL; Pectinase [H]

Number of amino acids: Translated: 560; Mature: 560

Protein sequence:

>560_residues
MKTNLTNLFCTALTILAVSFSVPVLAQKASDTYKDIEFKMAKIKEPVIPKNSVNIKDFGAVNGGYVLNTKAFADAIDAVS
KKGGGKVIIPPGIWLTGPIILKSNIELHAERGALIKFSTDKSLYPIIGTNFEGLNTWRCISPIYGKNLENIAFTGNGVWD
GSGEVWRQVKKSKLTESQWKKFVSSGGVLNKDKTSWYPSETFMNASKGADQNVRPDLKTKEEFETIHDFLRPVMVSIQNS
KRVLFDGPVFQNSPAWNIHPFMVEDLIVRNVTVRNPWYSQNGDGLDVECCKNVLVENSSFDVGDDAICIKSGKDKDGLER
GIPCENIIVRNNIVYHGHGGVTVGSEMSGGVKNLHVSNCTFMGTDVGLRFKSARGRGGVVENIFISDVFMTDIPSQAISF
NLYYGGKSIAETLEEGGDKIVNKAMPVTIETPQFKNISIKNITIKGAQQAVFLQGLPEMNLENIEISNLTVTAEKGFSII
DAKGIKITNAKLDIENSNVFEVYNVKNMSLKDVEFNSTSAKAVNINGEGNANIELVSSKKLDFSKTTTLGNDVPKGAVKF

Sequences:

>Translated_560_residues
MKTNLTNLFCTALTILAVSFSVPVLAQKASDTYKDIEFKMAKIKEPVIPKNSVNIKDFGAVNGGYVLNTKAFADAIDAVS
KKGGGKVIIPPGIWLTGPIILKSNIELHAERGALIKFSTDKSLYPIIGTNFEGLNTWRCISPIYGKNLENIAFTGNGVWD
GSGEVWRQVKKSKLTESQWKKFVSSGGVLNKDKTSWYPSETFMNASKGADQNVRPDLKTKEEFETIHDFLRPVMVSIQNS
KRVLFDGPVFQNSPAWNIHPFMVEDLIVRNVTVRNPWYSQNGDGLDVECCKNVLVENSSFDVGDDAICIKSGKDKDGLER
GIPCENIIVRNNIVYHGHGGVTVGSEMSGGVKNLHVSNCTFMGTDVGLRFKSARGRGGVVENIFISDVFMTDIPSQAISF
NLYYGGKSIAETLEEGGDKIVNKAMPVTIETPQFKNISIKNITIKGAQQAVFLQGLPEMNLENIEISNLTVTAEKGFSII
DAKGIKITNAKLDIENSNVFEVYNVKNMSLKDVEFNSTSAKAVNINGEGNANIELVSSKKLDFSKTTTLGNDVPKGAVKF
>Mature_560_residues
MKTNLTNLFCTALTILAVSFSVPVLAQKASDTYKDIEFKMAKIKEPVIPKNSVNIKDFGAVNGGYVLNTKAFADAIDAVS
KKGGGKVIIPPGIWLTGPIILKSNIELHAERGALIKFSTDKSLYPIIGTNFEGLNTWRCISPIYGKNLENIAFTGNGVWD
GSGEVWRQVKKSKLTESQWKKFVSSGGVLNKDKTSWYPSETFMNASKGADQNVRPDLKTKEEFETIHDFLRPVMVSIQNS
KRVLFDGPVFQNSPAWNIHPFMVEDLIVRNVTVRNPWYSQNGDGLDVECCKNVLVENSSFDVGDDAICIKSGKDKDGLER
GIPCENIIVRNNIVYHGHGGVTVGSEMSGGVKNLHVSNCTFMGTDVGLRFKSARGRGGVVENIFISDVFMTDIPSQAISF
NLYYGGKSIAETLEEGGDKIVNKAMPVTIETPQFKNISIKNITIKGAQQAVFLQGLPEMNLENIEISNLTVTAEKGFSII
DAKGIKITNAKLDIENSNVFEVYNVKNMSLKDVEFNSTSAKAVNINGEGNANIELVSSKKLDFSKTTTLGNDVPKGAVKF

Specific function: Seems to regulates the surface properties of the bacterium in the presence of plant cells or plant cell extracts [H]

COG id: COG5434

COG function: function code M; Endopolygalacturonase

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 4 PbH1 repeats [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000743
- InterPro:   IPR006626
- InterPro:   IPR012334
- InterPro:   IPR011050 [H]

Pfam domain/function: PF00295 Glyco_hydro_28 [H]

EC number: =3.2.1.15 [H]

Molecular weight: Translated: 61296; Mature: 61296

Theoretical pI: Translated: 8.25; Mature: 8.25

Prosite motif: PS00502 POLYGALACTURONASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTNLTNLFCTALTILAVSFSVPVLAQKASDTYKDIEFKMAKIKEPVIPKNSVNIKDFGA
CCCCHHHHHHHHHHHHHHHHCCCCEECCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCC
VNGGYVLNTKAFADAIDAVSKKGGGKVIIPPGIWLTGPIILKSNIELHAERGALIKFSTD
CCCCEEEECHHHHHHHHHHHCCCCCEEEECCCCEEECCEEEECCCEEEECCCEEEEEECC
KSLYPIIGTNFEGLNTWRCISPIYGKNLENIAFTGNGVWDGSGEVWRQVKKSKLTESQWK
CCCCEEECCCCCCCCCEEEECHHHCCCCCEEEEECCEEECCCHHHHHHHHHHCCCHHHHH
KFVSSGGVLNKDKTSWYPSETFMNASKGADQNVRPDLKTKEEFETIHDFLRPVMVSIQNS
HHHHCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHEECCC
KRVLFDGPVFQNSPAWNIHPFMVEDLIVRNVTVRNPWYSQNGDGLDVECCKNVLVENSSF
CEEEEECCEECCCCCCCCCHHHHHHHHHHCEEECCCCCCCCCCCCCHHHHHHHHCCCCCC
DVGDDAICIKSGKDKDGLERGIPCENIIVRNNIVYHGHGGVTVGSEMSGGVKNLHVSNCT
CCCCCEEEEECCCCCCHHHHCCCCCCEEEECCEEEECCCCEEECCCCCCCCCEEEECCEE
FMGTDVGLRFKSARGRGGVVENIFISDVFMTDIPSQAISFNLYYGGKSIAETLEEGGDKI
EEECCCCEEEECCCCCCCCEEEEEEEEEEHHCCCCCCEEEEEEECCHHHHHHHHHCCHHH
VNKAMPVTIETPQFKNISIKNITIKGAQQAVFLQGLPEMNLENIEISNLTVTAEKGFSII
HHCCCCEEEECCCCCCEEEEEEEECCCCEEEEECCCCCCCCCEEEEEEEEEECCCCCEEE
DAKGIKITNAKLDIENSNVFEVYNVKNMSLKDVEFNSTSAKAVNINGEGNANIELVSSKK
ECCCEEEEEEEEEECCCCEEEEEECCCCEEEEEEECCCCCEEEEECCCCCCEEEEEECCC
LDFSKTTTLGNDVPKGAVKF
CCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKTNLTNLFCTALTILAVSFSVPVLAQKASDTYKDIEFKMAKIKEPVIPKNSVNIKDFGA
CCCCHHHHHHHHHHHHHHHHCCCCEECCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCC
VNGGYVLNTKAFADAIDAVSKKGGGKVIIPPGIWLTGPIILKSNIELHAERGALIKFSTD
CCCCEEEECHHHHHHHHHHHCCCCCEEEECCCCEEECCEEEECCCEEEECCCEEEEEECC
KSLYPIIGTNFEGLNTWRCISPIYGKNLENIAFTGNGVWDGSGEVWRQVKKSKLTESQWK
CCCCEEECCCCCCCCCEEEECHHHCCCCCEEEEECCEEECCCHHHHHHHHHHCCCHHHHH
KFVSSGGVLNKDKTSWYPSETFMNASKGADQNVRPDLKTKEEFETIHDFLRPVMVSIQNS
HHHHCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHEECCC
KRVLFDGPVFQNSPAWNIHPFMVEDLIVRNVTVRNPWYSQNGDGLDVECCKNVLVENSSF
CEEEEECCEECCCCCCCCCHHHHHHHHHHCEEECCCCCCCCCCCCCHHHHHHHHCCCCCC
DVGDDAICIKSGKDKDGLERGIPCENIIVRNNIVYHGHGGVTVGSEMSGGVKNLHVSNCT
CCCCCEEEEECCCCCCHHHHCCCCCCEEEECCEEEECCCCEEECCCCCCCCCEEEECCEE
FMGTDVGLRFKSARGRGGVVENIFISDVFMTDIPSQAISFNLYYGGKSIAETLEEGGDKI
EEECCCCEEEECCCCCCCCEEEEEEEEEEHHCCCCCCEEEEEEECCHHHHHHHHHCCHHH
VNKAMPVTIETPQFKNISIKNITIKGAQQAVFLQGLPEMNLENIEISNLTVTAEKGFSII
HHCCCCEEEECCCCCCEEEEEEEECCCCEEEEECCCCCCCCCEEEEEEEEEECCCCCEEE
DAKGIKITNAKLDIENSNVFEVYNVKNMSLKDVEFNSTSAKAVNINGEGNANIELVSSKK
ECCCEEEEEEEEEECCCCEEEEEECCCCEEEEEEECCCCCEEEEECCCCCCEEEEEECCC
LDFSKTTTLGNDVPKGAVKF
CCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1860822 [H]