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The map label for this gene is miaA

Identifier: 146301195

GI number: 146301195

Start: 4119658

End: 4120587

Strand: Direct

Name: miaA

Synonym: Fjoh_3453

Alternate gene names: 146301195

Gene position: 4119658-4120587 (Clockwise)

Preceding gene: 146301194

Following gene: 146301196

Centisome position: 67.57

GC content: 33.55

Gene sequence:

>930_bases
ATGAAATATCTAATTACCATTGTCGGACCAACAGCTATAGGCAAAACAGCCTTGAGCATTGCTTTGGCACAACATTTTAA
ATGCGAAATCGTTTCTTGCGACAGCCGTCAATTTTTCAAAGAAATGACCATTGGAACCGCAGTTCCAAGTCAGGAAGAAC
TCAATTCTGCCAAGCATCATTTCATTCAAAATAAATCTATTTTCGAAAATTATACCGTTGGCGATTACGAAAAAGAAGCT
TTAGCCAAAATTGAAGAACTGTTTGAAGCCAATGATTTTGTTATTCTTATTGGTGGATCAGGTTTATATGTAGATGCCAT
TCTAAAAGGTTTTGATGAATTTCCAGAAATAGATCCAAATGTTCGCGCCGAAGTAAATACAAATTATGAAAAACTTGGAA
TCGAATATCTTCAAGAACAATTAAAAAACCTAGATCCAGAATATTATCAAAAAATAACTTTGGAAAATCCGCAGACATTG
CAAAACCCGCAACGAATGATGCGTTTTGTAGAAGTCTGCATTGGATCTCAAAAACCATATTCTTCTTTTTTAAACCTAAA
GAAAAATAACCGAAACTTCACTCCTATTCTAATTGGTTTAGATGCCGACAGAGAAATCATTTACAGCAGAATCAACCAGC
GTGTTGATATCATGATGAATGAAGGATTATTAAAAGAAGCCGAAACACTTTATCCCAACAAAGCGTTAAATGCACTCCAA
ACCGTCGGTTATAGAGAATTATTTAGTTATTTTGACGGAGATTTCACTTTGCCATTTGCCATTGAAGAAATCAAAAAAAA
CACAAGAAGATTTTCCAAAAGACAATTAACGTGGTTCAAACGAAATGAAAACACAAAATGGTTTGATTATTCGACTGACA
GAAATGAGATTATTCATTACATCGTAGAAAATCTAAAATCATCAATATAA

Upstream 100 bases:

>100_bases
TTTTAAGGCATAAAAGGAGCTTCCGTTGGTCGCTCTTTTACACCAAAAAAATTCAACAAATATAAGTCCGGGGTTTCCAT
TTCCATCTGGGCTAAAAATT

Downstream 100 bases:

>100_bases
AATCTAAAATTAACAATGCCAATATCTCCCAATTTTACATCAGTTTTAAGCAAAGACTGGGAAATTAATTTCACGCAGTG
CACACCAAACGGCTACTTAA

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase

Number of amino acids: Translated: 309; Mature: 309

Protein sequence:

>309_residues
MKYLITIVGPTAIGKTALSIALAQHFKCEIVSCDSRQFFKEMTIGTAVPSQEELNSAKHHFIQNKSIFENYTVGDYEKEA
LAKIEELFEANDFVILIGGSGLYVDAILKGFDEFPEIDPNVRAEVNTNYEKLGIEYLQEQLKNLDPEYYQKITLENPQTL
QNPQRMMRFVEVCIGSQKPYSSFLNLKKNNRNFTPILIGLDADREIIYSRINQRVDIMMNEGLLKEAETLYPNKALNALQ
TVGYRELFSYFDGDFTLPFAIEEIKKNTRRFSKRQLTWFKRNENTKWFDYSTDRNEIIHYIVENLKSSI

Sequences:

>Translated_309_residues
MKYLITIVGPTAIGKTALSIALAQHFKCEIVSCDSRQFFKEMTIGTAVPSQEELNSAKHHFIQNKSIFENYTVGDYEKEA
LAKIEELFEANDFVILIGGSGLYVDAILKGFDEFPEIDPNVRAEVNTNYEKLGIEYLQEQLKNLDPEYYQKITLENPQTL
QNPQRMMRFVEVCIGSQKPYSSFLNLKKNNRNFTPILIGLDADREIIYSRINQRVDIMMNEGLLKEAETLYPNKALNALQ
TVGYRELFSYFDGDFTLPFAIEEIKKNTRRFSKRQLTWFKRNENTKWFDYSTDRNEIIHYIVENLKSSI
>Mature_309_residues
MKYLITIVGPTAIGKTALSIALAQHFKCEIVSCDSRQFFKEMTIGTAVPSQEELNSAKHHFIQNKSIFENYTVGDYEKEA
LAKIEELFEANDFVILIGGSGLYVDAILKGFDEFPEIDPNVRAEVNTNYEKLGIEYLQEQLKNLDPEYYQKITLENPQTL
QNPQRMMRFVEVCIGSQKPYSSFLNLKKNNRNFTPILIGLDADREIIYSRINQRVDIMMNEGLLKEAETLYPNKALNALQ
TVGYRELFSYFDGDFTLPFAIEEIKKNTRRFSKRQLTWFKRNENTKWFDYSTDRNEIIHYIVENLKSSI

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A)

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family

Homologues:

Organism=Homo sapiens, GI31581534, Length=324, Percent_Identity=26.2345679012346, Blast_Score=107, Evalue=2e-23,
Organism=Escherichia coli, GI1790613, Length=308, Percent_Identity=31.8181818181818, Blast_Score=171, Evalue=8e-44,
Organism=Caenorhabditis elegans, GI25144712, Length=306, Percent_Identity=23.5294117647059, Blast_Score=75, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6324848, Length=301, Percent_Identity=27.906976744186, Blast_Score=113, Evalue=4e-26,
Organism=Drosophila melanogaster, GI24649830, Length=337, Percent_Identity=26.7062314540059, Blast_Score=109, Evalue=3e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAA_FLAJ1 (A5FE96)

Other databases:

- EMBL:   CP000685
- RefSeq:   YP_001195786.1
- ProteinModelPortal:   A5FE96
- SMR:   A5FE96
- STRING:   A5FE96
- GeneID:   5089654
- GenomeReviews:   CP000685_GR
- KEGG:   fjo:Fjoh_3453
- eggNOG:   COG0324
- HOGENOM:   HBG559459
- OMA:   VNADSMQ
- ProtClustDB:   PRK00091
- BioCyc:   FJOH376686:FJOH_3453-MONOMER
- HAMAP:   MF_00185
- InterPro:   IPR002627
- InterPro:   IPR018022
- PANTHER:   PTHR11088
- TIGRFAMs:   TIGR00174

Pfam domain/function: PF01715 IPPT

EC number: =2.5.1.75

Molecular weight: Translated: 35930; Mature: 35930

Theoretical pI: Translated: 5.31; Mature: 5.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYLITIVGPTAIGKTALSIALAQHFKCEIVSCDSRQFFKEMTIGTAVPSQEELNSAKHH
CCEEEEEECCHHHHHHHHHHHHHHHCEEEEEECCHHHHHHHHHHCCCCCCHHHHHHHHHH
FIQNKSIFENYTVGDYEKEALAKIEELFEANDFVILIGGSGLYVDAILKGFDEFPEIDPN
HHCCCHHHCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHCCCCCCC
VRAEVNTNYEKLGIEYLQEQLKNLDPEYYQKITLENPQTLQNPQRMMRFVEVCIGSQKPY
CEEEECCCHHHHHHHHHHHHHHCCCHHHHHEECCCCCHHHCCHHHHHHHHHHHHCCCCCH
SSFLNLKKNNRNFTPILIGLDADREIIYSRINQRVDIMMNEGLLKEAETLYPNKALNALQ
HHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHEEHCCCCHHHHHHHHCCHHHHHHHH
TVGYRELFSYFDGDFTLPFAIEEIKKNTRRFSKRQLTWFKRNENTKWFDYSTDRNEIIHY
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHEEECCCCEEEECCCCHHHHHHH
IVENLKSSI
HHHHHHHCC
>Mature Secondary Structure
MKYLITIVGPTAIGKTALSIALAQHFKCEIVSCDSRQFFKEMTIGTAVPSQEELNSAKHH
CCEEEEEECCHHHHHHHHHHHHHHHCEEEEEECCHHHHHHHHHHCCCCCCHHHHHHHHHH
FIQNKSIFENYTVGDYEKEALAKIEELFEANDFVILIGGSGLYVDAILKGFDEFPEIDPN
HHCCCHHHCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHCCCCCCC
VRAEVNTNYEKLGIEYLQEQLKNLDPEYYQKITLENPQTLQNPQRMMRFVEVCIGSQKPY
CEEEECCCHHHHHHHHHHHHHHCCCHHHHHEECCCCCHHHCCHHHHHHHHHHHHCCCCCH
SSFLNLKKNNRNFTPILIGLDADREIIYSRINQRVDIMMNEGLLKEAETLYPNKALNALQ
HHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHEEHCCCCHHHHHHHHCCHHHHHHHH
TVGYRELFSYFDGDFTLPFAIEEIKKNTRRFSKRQLTWFKRNENTKWFDYSTDRNEIIHY
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHEEECCCCEEEECCCCHHHHHHH
IVENLKSSI
HHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA