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The map label for this gene is fadI [H]

Identifier: 146301065

GI number: 146301065

Start: 3967971

End: 3969251

Strand: Direct

Name: fadI [H]

Synonym: Fjoh_3321

Alternate gene names: 146301065

Gene position: 3967971-3969251 (Clockwise)

Preceding gene: 146301063

Following gene: 146301067

Centisome position: 65.08

GC content: 39.03

Gene sequence:

>1281_bases
ATGAATACCAGCAAAACTGTTAGAAAAGTCGCTATTGTAGGTTACAACCGAATTCCTTTTGCAAGAGCAAATACTGCTTA
TGCATCTGTTGGAAATCAGGAAATGATGACTGCCGCCCTAAACGGACTTGTCGATAAGTATAATCTGCAGGGCCAATTAT
TAGGTGAAGTTGCCGGAGGCGCTGTAATCAAACATACTTACGATAATAATTTAATCAGAGAGTGTGTAATGAAAACCGCT
CTAGATCCCGCCACTCCTGCCTGCGATTTACAACAGGCCTGCGATACCGGAATTGAAAGTGCAGTTTATATTGCTAATAA
AATTGCTTTGGGACAAATTGAATCTGGAATTGCCGGCGGTGTAGATTCCATCAGCGATATACCAATGGCTGTAAGTGAAA
AACTAAGGAAAGTTTTGTTAGAAGCCCGTCAGGCAAAATCGCTGGGCGGAAAAATCAAAGCATTTTTAAAATTAAGCCCG
AAAGACCTTTCGCCTTTAGTTCCTAAAAATGAAGAACAGCAAACGGGACTTTCAATGGGCGGACATACTGAGATTACGGC
AAAATATTATAAAATTTCAAGAGAAGATCAGGATAATTTTGCACTTAAAAGTCATTTGAATATGGCAAAAGCGTATGATG
AAGGCTTTTTTAATGATATGATTACGCCCTTTAACGGACTGGAAAAAGATAATAATCTGAGAAAAGACAGCTCGATTGAA
AAACTGGCAAAACTAAAACCGGCATTCGATAAAGTAAACGGAACTTTAACTGCCGGAAATTCTACTCCCCTTACTGATGG
TGCTTCCTGTATACTTTTAGCGAGTGAAGAATGGGCAAAAGAACGCGGACTTCCTATTTTGGCATATATTACTTTTGCTG
AAGCTGCAGCGATAGAATATGTAAAAAATCAACAAAATCTTTTGCTGGCACCTTTATTTGCAGCAAGCCGAATGCTTGAA
AAAGCTGAATTAAATTTGCAGGATTTTGATTATTATGAAATTCATGAAGCTTTTGCTGCACAAGTATTGGCAACTTTAAA
AATTTGGGAAAGCCCGGAATTAAGTGCAGAGTTAGGCTTAAAGAAAACACTTGGTGCAATAGACCGCGAAAAATTAAATG
TAAAAGGAAGCAGTCTTGCAGCCGCACACCCTTTTGCAGCAACTGGAGGCAGAATAATTGGCGTAATGGCAAAATTACTA
AACGAAAAAGGTTCAGGTCGAGGATTTGTTTCGATTTGTGCTGCTGGCGGACAAGGTGTTACGATGATAATCGAAAAGTA
A

Upstream 100 bases:

>100_bases
TTTTAGTGAAATAATTCTAAACCCGAATTTTATTGCTAATTTTTAATTAAGTCTGCTTATCTTTAATCCTGCAAAAACAA
ATCCGTCAAAACAAAATCTT

Downstream 100 bases:

>100_bases
TTATTTTTGATATTAAAAACCCGGCAGATTTTAAAAACCTGCCGGGTTTACTACATACGAGAATGTTCAACCGTCGTAAT
TATAGTTTATCTTAAAAAGC

Product: acetyl-CoA acetyltransferase

Products: NA

Alternate protein names: ACSs; Acetyl-CoA acyltransferase; Acyl-CoA ligase; Beta-ketothiolase; Fatty acid oxidation complex subunit beta [H]

Number of amino acids: Translated: 426; Mature: 426

Protein sequence:

>426_residues
MNTSKTVRKVAIVGYNRIPFARANTAYASVGNQEMMTAALNGLVDKYNLQGQLLGEVAGGAVIKHTYDNNLIRECVMKTA
LDPATPACDLQQACDTGIESAVYIANKIALGQIESGIAGGVDSISDIPMAVSEKLRKVLLEARQAKSLGGKIKAFLKLSP
KDLSPLVPKNEEQQTGLSMGGHTEITAKYYKISREDQDNFALKSHLNMAKAYDEGFFNDMITPFNGLEKDNNLRKDSSIE
KLAKLKPAFDKVNGTLTAGNSTPLTDGASCILLASEEWAKERGLPILAYITFAEAAAIEYVKNQQNLLLAPLFAASRMLE
KAELNLQDFDYYEIHEAFAAQVLATLKIWESPELSAELGLKKTLGAIDREKLNVKGSSLAAAHPFAATGGRIIGVMAKLL
NEKGSGRGFVSICAAGGQGVTMIIEK

Sequences:

>Translated_426_residues
MNTSKTVRKVAIVGYNRIPFARANTAYASVGNQEMMTAALNGLVDKYNLQGQLLGEVAGGAVIKHTYDNNLIRECVMKTA
LDPATPACDLQQACDTGIESAVYIANKIALGQIESGIAGGVDSISDIPMAVSEKLRKVLLEARQAKSLGGKIKAFLKLSP
KDLSPLVPKNEEQQTGLSMGGHTEITAKYYKISREDQDNFALKSHLNMAKAYDEGFFNDMITPFNGLEKDNNLRKDSSIE
KLAKLKPAFDKVNGTLTAGNSTPLTDGASCILLASEEWAKERGLPILAYITFAEAAAIEYVKNQQNLLLAPLFAASRMLE
KAELNLQDFDYYEIHEAFAAQVLATLKIWESPELSAELGLKKTLGAIDREKLNVKGSSLAAAHPFAATGGRIIGVMAKLL
NEKGSGRGFVSICAAGGQGVTMIIEK
>Mature_426_residues
MNTSKTVRKVAIVGYNRIPFARANTAYASVGNQEMMTAALNGLVDKYNLQGQLLGEVAGGAVIKHTYDNNLIRECVMKTA
LDPATPACDLQQACDTGIESAVYIANKIALGQIESGIAGGVDSISDIPMAVSEKLRKVLLEARQAKSLGGKIKAFLKLSP
KDLSPLVPKNEEQQTGLSMGGHTEITAKYYKISREDQDNFALKSHLNMAKAYDEGFFNDMITPFNGLEKDNNLRKDSSIE
KLAKLKPAFDKVNGTLTAGNSTPLTDGASCILLASEEWAKERGLPILAYITFAEAAAIEYVKNQQNLLLAPLFAASRMLE
KAELNLQDFDYYEIHEAFAAQVLATLKIWESPELSAELGLKKTLGAIDREKLNVKGSSLAAAHPFAATGGRIIGVMAKLL
NEKGSGRGFVSICAAGGQGVTMIIEK

Specific function: Catalyzes the final step of fatty acid oxidation in which acetyl-CoA is released and the CoA ester of a fatty acid two carbons shorter is formed [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI4504327, Length=427, Percent_Identity=37.0023419203747, Blast_Score=259, Evalue=3e-69,
Organism=Homo sapiens, GI167614485, Length=430, Percent_Identity=28.8372093023256, Blast_Score=154, Evalue=1e-37,
Organism=Homo sapiens, GI4557237, Length=431, Percent_Identity=26.4501160092807, Blast_Score=153, Evalue=3e-37,
Organism=Homo sapiens, GI4501853, Length=431, Percent_Identity=27.6102088167053, Blast_Score=151, Evalue=1e-36,
Organism=Homo sapiens, GI148539872, Length=426, Percent_Identity=27.9342723004695, Blast_Score=147, Evalue=1e-35,
Organism=Escherichia coli, GI1788683, Length=424, Percent_Identity=37.9716981132075, Blast_Score=273, Evalue=2e-74,
Organism=Escherichia coli, GI87082165, Length=429, Percent_Identity=28.4382284382284, Blast_Score=162, Evalue=5e-41,
Organism=Escherichia coli, GI1787663, Length=432, Percent_Identity=29.8611111111111, Blast_Score=161, Evalue=9e-41,
Organism=Escherichia coli, GI48994986, Length=434, Percent_Identity=27.6497695852535, Blast_Score=151, Evalue=8e-38,
Organism=Escherichia coli, GI1788554, Length=431, Percent_Identity=28.7703016241299, Blast_Score=148, Evalue=6e-37,
Organism=Caenorhabditis elegans, GI17551802, Length=433, Percent_Identity=34.1801385681293, Blast_Score=227, Evalue=7e-60,
Organism=Caenorhabditis elegans, GI133906874, Length=426, Percent_Identity=28.8732394366197, Blast_Score=144, Evalue=7e-35,
Organism=Caenorhabditis elegans, GI25147385, Length=427, Percent_Identity=26.463700234192, Blast_Score=131, Evalue=7e-31,
Organism=Caenorhabditis elegans, GI17535921, Length=430, Percent_Identity=26.2790697674419, Blast_Score=129, Evalue=3e-30,
Organism=Saccharomyces cerevisiae, GI6322031, Length=440, Percent_Identity=30.2272727272727, Blast_Score=159, Evalue=9e-40,
Organism=Saccharomyces cerevisiae, GI6325229, Length=435, Percent_Identity=27.3563218390805, Blast_Score=146, Evalue=6e-36,
Organism=Drosophila melanogaster, GI17137578, Length=429, Percent_Identity=37.0629370629371, Blast_Score=251, Evalue=5e-67,
Organism=Drosophila melanogaster, GI24655093, Length=427, Percent_Identity=26.9320843091335, Blast_Score=152, Evalue=5e-37,
Organism=Drosophila melanogaster, GI17648125, Length=433, Percent_Identity=29.3302540415704, Blast_Score=145, Evalue=7e-35,
Organism=Drosophila melanogaster, GI24640423, Length=433, Percent_Identity=25.4041570438799, Blast_Score=134, Evalue=1e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012806
- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: =2.3.1.16 [H]

Molecular weight: Translated: 45865; Mature: 45865

Theoretical pI: Translated: 7.45; Mature: 7.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTSKTVRKVAIVGYNRIPFARANTAYASVGNQEMMTAALNGLVDKYNLQGQLLGEVAGG
CCCHHHHHEEEEECCCCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHHCC
AVIKHTYDNNLIRECVMKTALDPATPACDLQQACDTGIESAVYIANKIALGQIESGIAGG
EEEEEECCCHHHHHHHHHHCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCC
VDSISDIPMAVSEKLRKVLLEARQAKSLGGKIKAFLKLSPKDLSPLVPKNEEQQTGLSMG
CCCHHCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCHHCCCCCC
GHTEITAKYYKISREDQDNFALKSHLNMAKAYDEGFFNDMITPFNGLEKDNNLRKDSSIE
CCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHH
KLAKLKPAFDKVNGTLTAGNSTPLTDGASCILLASEEWAKERGLPILAYITFAEAAAIEY
HHHHHCCHHHHCCCEEECCCCCCCCCCCCEEEEECHHHHHHCCCEEEEEEHHHHHHHHHH
VKNQQNLLLAPLFAASRMLEKAELNLQDFDYYEIHEAFAAQVLATLKIWESPELSAELGL
HHCCCCEEEHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHCH
KKTLGAIDREKLNVKGSSLAAAHPFAATGGRIIGVMAKLLNEKGSGRGFVSICAAGGQGV
HHHHCCCCHHHCCCCCCCEECCCCCHHCCCHHHHHHHHHHCCCCCCCCEEEEEECCCCCE
TMIIEK
EEEEEC
>Mature Secondary Structure
MNTSKTVRKVAIVGYNRIPFARANTAYASVGNQEMMTAALNGLVDKYNLQGQLLGEVAGG
CCCHHHHHEEEEECCCCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHHCC
AVIKHTYDNNLIRECVMKTALDPATPACDLQQACDTGIESAVYIANKIALGQIESGIAGG
EEEEEECCCHHHHHHHHHHCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCC
VDSISDIPMAVSEKLRKVLLEARQAKSLGGKIKAFLKLSPKDLSPLVPKNEEQQTGLSMG
CCCHHCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCHHCCCCCC
GHTEITAKYYKISREDQDNFALKSHLNMAKAYDEGFFNDMITPFNGLEKDNNLRKDSSIE
CCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHH
KLAKLKPAFDKVNGTLTAGNSTPLTDGASCILLASEEWAKERGLPILAYITFAEAAAIEY
HHHHHCCHHHHCCCEEECCCCCCCCCCCCEEEEECHHHHHHCCCEEEEEEHHHHHHHHHH
VKNQQNLLLAPLFAASRMLEKAELNLQDFDYYEIHEAFAAQVLATLKIWESPELSAELGL
HHCCCCEEEHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHCH
KKTLGAIDREKLNVKGSSLAAAHPFAATGGRIIGVMAKLLNEKGSGRGFVSICAAGGQGV
HHHHCCCCHHHCCCCCCCEECCCCCHHCCCHHHHHHHHHHCCCCCCCCEEEEEECCCCCE
TMIIEK
EEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA