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The map label for this gene is qor [C]

Identifier: 146301053

GI number: 146301053

Start: 3952636

End: 3953649

Strand: Reverse

Name: qor [C]

Synonym: Fjoh_3309

Alternate gene names: 146301053

Gene position: 3953649-3952636 (Counterclockwise)

Preceding gene: 146301056

Following gene: 146301052

Centisome position: 64.85

GC content: 36.39

Gene sequence:

>1014_bases
ATGAAAGCAATAGGATTCAAAACCTCATTATCAATAGAAGAAAAAGACAGTTTTATTGAATTTGAAACTGTAAAACCAAC
GCCTGGTCCGCACGATTTATTAGTAAAAATTGATGCGATTTCAGTAAATCCGGTCGATTTTAAAATTCGTCAGAGCGCCG
CAAAAGACACCGTATTAGAAACCCCAAAAATTATTGGCTGGGATGCAGTTGGAATTGTGCAGGCCGTTGGCGAAAAAGTA
ACTTTATTTGATGTTGGCGACTTAGTATATTATGCCGGAGATATCACCAAACAAGGAAGCAACGCCGAATACCAAATTAT
TGACGAAAGAATTGTTGGAAATAAACCAACATCCTTAACTGATGCCGAAGCAGCTGCTATTCCGTTAACAGGATTAACAG
CTTGGGAAATTCTGTTTGACAGAATCCGAATTAATCCTGAAAAAGACAAAGGAAAATCAATTCTTATTATTGGAGGTGCC
GGTGGTGTGGGCTCTATCGCTATTCAGCTGGCTAAGAAAATTGCAGGATTAACAGTTATTGCCACCGCTTCACGTCCGGA
AACCATTGACCGGTGTAAAAAACAGGGAGCTGATTTTGTTGTCGATCATAAAAACTTAATTGAATCGGTTAGAGAAGCTG
GTTTTCAAAACGTAGATTTCATTTTAGACTTTGTTGATACCAATGCGTATTGGGACATTATGGCCGAATTAATAAAACCT
CAGGGACATATTGCTTCAATTACCGGAAGTGCTGAACCCGTTGTTTTGAATAAATTGAAAGGTAAAAGTGTTTCATTTTC
ATGGGAATTGATGTACACACGTTCAATGTTTCAAACAGAAGACATGATCGATCAGCATCATATTTTAAACAAAATAGCCG
ATCTATTAGATGAAGGTGTTTTAAAAACGACTTTAAAAGAAACATATAAAGGACTTACTGCTGAAAATTTAATAAAAGCA
CATCAGCTTTTAGAGTCCGGAAAAACAATTGGAAAAATTGCAATAAAATTTTAA

Upstream 100 bases:

>100_bases
AAATGCTGAATTTGTACAAATATTGCATAAAATTATACCAATTATAAGAATCAAACCGCTTGTATCTTTGTTAGTATATT
TAAACCAAAATTTAGAAACA

Downstream 100 bases:

>100_bases
AAACAGCATTCTGGACGGTGTATGATATATAAGGTTAGTTATTTAGAATTAATTTAAATAACGTATTTTTTATCTTAACA
ATTTTTGTATTTTAGCGGCC

Product: alcohol dehydrogenase

Products: NADP(+); Semiquinone. [C]

Alternate protein names: NA

Number of amino acids: Translated: 337; Mature: 337

Protein sequence:

>337_residues
MKAIGFKTSLSIEEKDSFIEFETVKPTPGPHDLLVKIDAISVNPVDFKIRQSAAKDTVLETPKIIGWDAVGIVQAVGEKV
TLFDVGDLVYYAGDITKQGSNAEYQIIDERIVGNKPTSLTDAEAAAIPLTGLTAWEILFDRIRINPEKDKGKSILIIGGA
GGVGSIAIQLAKKIAGLTVIATASRPETIDRCKKQGADFVVDHKNLIESVREAGFQNVDFILDFVDTNAYWDIMAELIKP
QGHIASITGSAEPVVLNKLKGKSVSFSWELMYTRSMFQTEDMIDQHHILNKIADLLDEGVLKTTLKETYKGLTAENLIKA
HQLLESGKTIGKIAIKF

Sequences:

>Translated_337_residues
MKAIGFKTSLSIEEKDSFIEFETVKPTPGPHDLLVKIDAISVNPVDFKIRQSAAKDTVLETPKIIGWDAVGIVQAVGEKV
TLFDVGDLVYYAGDITKQGSNAEYQIIDERIVGNKPTSLTDAEAAAIPLTGLTAWEILFDRIRINPEKDKGKSILIIGGA
GGVGSIAIQLAKKIAGLTVIATASRPETIDRCKKQGADFVVDHKNLIESVREAGFQNVDFILDFVDTNAYWDIMAELIKP
QGHIASITGSAEPVVLNKLKGKSVSFSWELMYTRSMFQTEDMIDQHHILNKIADLLDEGVLKTTLKETYKGLTAENLIKA
HQLLESGKTIGKIAIKF
>Mature_337_residues
MKAIGFKTSLSIEEKDSFIEFETVKPTPGPHDLLVKIDAISVNPVDFKIRQSAAKDTVLETPKIIGWDAVGIVQAVGEKV
TLFDVGDLVYYAGDITKQGSNAEYQIIDERIVGNKPTSLTDAEAAAIPLTGLTAWEILFDRIRINPEKDKGKSILIIGGA
GGVGSIAIQLAKKIAGLTVIATASRPETIDRCKKQGADFVVDHKNLIESVREAGFQNVDFILDFVDTNAYWDIMAELIKP
QGHIASITGSAEPVVLNKLKGKSVSFSWELMYTRSMFQTEDMIDQHHILNKIADLLDEGVLKTTLKETYKGLTAENLIKA
HQLLESGKTIGKIAIKF

Specific function: Unknown

COG id: COG0604

COG function: function code CR; NADPH:quinone reductase and related Zn-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family. Quinone oxidoreductase subfamily [H]

Homologues:

Organism=Homo sapiens, GI47519420, Length=332, Percent_Identity=28.3132530120482, Blast_Score=112, Evalue=5e-25,
Organism=Homo sapiens, GI194239674, Length=203, Percent_Identity=31.0344827586207, Blast_Score=89, Evalue=5e-18,
Organism=Homo sapiens, GI13236495, Length=203, Percent_Identity=31.0344827586207, Blast_Score=89, Evalue=5e-18,
Organism=Homo sapiens, GI194239676, Length=191, Percent_Identity=31.9371727748691, Blast_Score=87, Evalue=3e-17,
Organism=Homo sapiens, GI24308257, Length=262, Percent_Identity=27.0992366412214, Blast_Score=82, Evalue=9e-16,
Organism=Homo sapiens, GI22538446, Length=328, Percent_Identity=26.5243902439024, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI22538444, Length=328, Percent_Identity=26.5243902439024, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI18379349, Length=219, Percent_Identity=27.8538812785388, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI28557745, Length=336, Percent_Identity=26.7857142857143, Blast_Score=70, Evalue=2e-12,
Organism=Escherichia coli, GI1790485, Length=315, Percent_Identity=28.2539682539683, Blast_Score=79, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI71987554, Length=242, Percent_Identity=28.5123966942149, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17507255, Length=239, Percent_Identity=28.8702928870293, Blast_Score=72, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6319520, Length=321, Percent_Identity=26.1682242990654, Blast_Score=65, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR014182
- InterPro:   IPR011032
- InterPro:   IPR016040
- InterPro:   IPR002364 [H]

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N [H]

EC number: 1.6.5.5 [C]

Molecular weight: Translated: 36868; Mature: 36868

Theoretical pI: Translated: 5.48; Mature: 5.48

Prosite motif: PS01162 QOR_ZETA_CRYSTAL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAIGFKTSLSIEEKDSFIEFETVKPTPGPHDLLVKIDAISVNPVDFKIRQSAAKDTVLE
CCCCCCEEECCCCCCCCEEEEEEECCCCCCCEEEEEEEEEEECCCCEEEHHHHHHHHHHC
TPKIIGWDAVGIVQAVGEKVTLFDVGDLVYYAGDITKQGSNAEYQIIDERIVGNKPTSLT
CCCEECCCHHHHHHHCCCEEEEEEECCEEEEECCCCCCCCCCEEEEEEHHHCCCCCCCCC
DAEAAAIPLTGLTAWEILFDRIRINPEKDKGKSILIIGGAGGVGSIAIQLAKKIAGLTVI
CCCCCEEECCCCHHHHHHHHHHCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHCCEEEE
ATASRPETIDRCKKQGADFVVDHKNLIESVREAGFQNVDFILDFVDTNAYWDIMAELIKP
EECCCCHHHHHHHHCCCCEEECCHHHHHHHHHCCCCCHHHEEEECCCCHHHHHHHHHHCC
QGHIASITGSAEPVVLNKLKGKSVSFSWELMYTRSMFQTEDMIDQHHILNKIADLLDEGV
CCCEEEECCCCCCEEEEECCCCCEEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCHH
LKTTLKETYKGLTAENLIKAHQLLESGKTIGKIAIKF
HHHHHHHHHCCCCHHHHHHHHHHHHCCCCEEEEEEEC
>Mature Secondary Structure
MKAIGFKTSLSIEEKDSFIEFETVKPTPGPHDLLVKIDAISVNPVDFKIRQSAAKDTVLE
CCCCCCEEECCCCCCCCEEEEEEECCCCCCCEEEEEEEEEEECCCCEEEHHHHHHHHHHC
TPKIIGWDAVGIVQAVGEKVTLFDVGDLVYYAGDITKQGSNAEYQIIDERIVGNKPTSLT
CCCEECCCHHHHHHHCCCEEEEEEECCEEEEECCCCCCCCCCEEEEEEHHHCCCCCCCCC
DAEAAAIPLTGLTAWEILFDRIRINPEKDKGKSILIIGGAGGVGSIAIQLAKKIAGLTVI
CCCCCEEECCCCHHHHHHHHHHCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHCCEEEE
ATASRPETIDRCKKQGADFVVDHKNLIESVREAGFQNVDFILDFVDTNAYWDIMAELIKP
EECCCCHHHHHHHHCCCCEEECCHHHHHHHHHCCCCCHHHEEEECCCCHHHHHHHHHHCC
QGHIASITGSAEPVVLNKLKGKSVSFSWELMYTRSMFQTEDMIDQHHILNKIADLLDEGV
CCCEEEECCCCCCEEEEECCCCCEEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCHH
LKTTLKETYKGLTAENLIKAHQLLESGKTIGKIAIKF
HHHHHHHHHCCCCHHHHHHHHHHHHCCCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NADPH [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.017 {9,10-phenanthrenequinone}} 0.0153 {2,6-dichlorophenolindophenol}} 0.013 {2,6-dichlorophenolindophenol}} 0.065 {1,4-benzoquinone}} 0.007 {NADPH}} 0.0069 {NADPH}} 0.26 {1,4-naphthoquinone}} 0.0024 {1,2-naphthoquinone}} [C]

Substrates: NADPH; Quinone [C]

Specific reaction: NADPH + Quinone = NADP(+) + Semiquinone. [C]

General reaction: Oxidation; Reduction [C]

Inhibitor: 2, 3-Dimercaptopropanol; 2, 5-Dichloro-3, 6-dihydroxy -1, 4-benzoquinone; 4-Hydroxy coumarin; 5, 5'-Dithiobis (2-nitrobenzoate); ADP; Cibacronblue3GA; Coumarin; Cu2+; Dicoumarol warfarin, coumarin; Dithiothreitol preincubation with 9, 10-phenanthrenequino

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11418146 [H]