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The map label for this gene is mscM [H]

Identifier: 146300596

GI number: 146300596

Start: 3377071

End: 3378330

Strand: Reverse

Name: mscM [H]

Synonym: Fjoh_2846

Alternate gene names: 146300596

Gene position: 3378330-3377071 (Counterclockwise)

Preceding gene: 146300597

Following gene: 146300594

Centisome position: 55.41

GC content: 33.81

Gene sequence:

>1260_bases
ATGCATAAGCTTTTGGATAAAATATTCAATTTTTTATATCCGCTTTTTAGAGACTGGGGAATGAACCGCAATTTTGCGTC
ATACATTAGTCTTGTTATTAATATTGCTATTATGCTGGTTTTGGCATATGCAATTTATTATATCGCCAAATTTGTTTTGG
TTACGCTTACAGCCGTTTTTGCCCAGCGTACCAAAACTAAATTTGACGATTATTTAATACACAACAAAACCACAAAATAT
ACAGCCTACTTAATTCCATTTTTCTTTATTTATAAAGCTGTTCCGGTTATTTTAGATAAGTATGAATACTGGGAACTGCT
TTTTGGAAAAATTGTGGGCATTTATATCGTTTTAATTAGTTTATGGATCATAAGGACCATTTTTAATGCTTTACGCGACT
ATTTAAAACAAAAACCGGAATACAGCGATAAACCTATCGACAGTTTTGTTCAGGTTATTATGATCGTACTTTGGATTTTT
GGAGTAGCGATGATTATTTCAACTTTATTCGGAATCAAAAAAGGCGAATTACTAACCATTTTAGGAACACTTTCGGCTAT
TATTATCCTTATTTTTAGAGATACTATTCTTGGATTTGTTTCGAGTGTTCAGGTTGCTATAAACGATATGGTTCGTATTG
GCGACTGGATTACAATGGATAAATTTGGTGCCGATGGTGATGTAATCGAAATCAATCTGACAACCGTAAAAGTCCGCAAC
TTCGATAATACCATTACTACGATTCCAACGTATGCCCTGAGTTCAGATTCTTTTCAGAACTGGCGCGGTATGCAGAAGTC
TGACGGAAGACGTATTAAAAGACATGTTTTAATAAAAAGCAGCAGTATTCGTTTTCTAAACAATGATGACTTGAATCACA
TGAAAGGAATACAGCTTATAACTTCGTATATCGAAACCAGACAAGCCGAAATTGAAAAATATAATGAGCTTAGAGGAATT
GATAAATCTCTTTTGCTTAATGGTCGTAATATGACCAATTTAGGTATGTTTAGAAAATATATTATGCAGTATTTGTTAGA
TCATCCGGGATTAAACAAAAATATGCATATTATGTGCCGTCAGCTGCAATCTACCGCACATGGTGTTCCGTTAGAAATTT
ATGTTTTTTCGAGCGATAAACGCTGGGCAAACTACGAATATATAATGTCGGATATTTTCGATCATGTTATGGCTTCTGTT
ATTTATTTCGATCTTGAAATATTCGAACTGCCATCACAGATTGGAAGATTGGATAGGTGA

Upstream 100 bases:

>100_bases
CAAAACTGGGATTTTAAAACTTTTGCAATTATACTAATCGCCTCTCTAATTCCGTTTGGAACATTTTATATCGAGAAAAA
GTATTTAGAAACTAAAGCAA

Downstream 100 bases:

>100_bases
TTTTTTTAGTTTTCAGTCTCAGTTTTCAGTCACAGATTTTACTTGTACTGAAAACTGCGACTGAAAACTGAAAACTACTT
CCTAACTTCTTCAAAAACAA

Product: mechanosensitive ion channel MscS

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 419; Mature: 419

Protein sequence:

>419_residues
MHKLLDKIFNFLYPLFRDWGMNRNFASYISLVINIAIMLVLAYAIYYIAKFVLVTLTAVFAQRTKTKFDDYLIHNKTTKY
TAYLIPFFFIYKAVPVILDKYEYWELLFGKIVGIYIVLISLWIIRTIFNALRDYLKQKPEYSDKPIDSFVQVIMIVLWIF
GVAMIISTLFGIKKGELLTILGTLSAIIILIFRDTILGFVSSVQVAINDMVRIGDWITMDKFGADGDVIEINLTTVKVRN
FDNTITTIPTYALSSDSFQNWRGMQKSDGRRIKRHVLIKSSSIRFLNNDDLNHMKGIQLITSYIETRQAEIEKYNELRGI
DKSLLLNGRNMTNLGMFRKYIMQYLLDHPGLNKNMHIMCRQLQSTAHGVPLEIYVFSSDKRWANYEYIMSDIFDHVMASV
IYFDLEIFELPSQIGRLDR

Sequences:

>Translated_419_residues
MHKLLDKIFNFLYPLFRDWGMNRNFASYISLVINIAIMLVLAYAIYYIAKFVLVTLTAVFAQRTKTKFDDYLIHNKTTKY
TAYLIPFFFIYKAVPVILDKYEYWELLFGKIVGIYIVLISLWIIRTIFNALRDYLKQKPEYSDKPIDSFVQVIMIVLWIF
GVAMIISTLFGIKKGELLTILGTLSAIIILIFRDTILGFVSSVQVAINDMVRIGDWITMDKFGADGDVIEINLTTVKVRN
FDNTITTIPTYALSSDSFQNWRGMQKSDGRRIKRHVLIKSSSIRFLNNDDLNHMKGIQLITSYIETRQAEIEKYNELRGI
DKSLLLNGRNMTNLGMFRKYIMQYLLDHPGLNKNMHIMCRQLQSTAHGVPLEIYVFSSDKRWANYEYIMSDIFDHVMASV
IYFDLEIFELPSQIGRLDR
>Mature_419_residues
MHKLLDKIFNFLYPLFRDWGMNRNFASYISLVINIAIMLVLAYAIYYIAKFVLVTLTAVFAQRTKTKFDDYLIHNKTTKY
TAYLIPFFFIYKAVPVILDKYEYWELLFGKIVGIYIVLISLWIIRTIFNALRDYLKQKPEYSDKPIDSFVQVIMIVLWIF
GVAMIISTLFGIKKGELLTILGTLSAIIILIFRDTILGFVSSVQVAINDMVRIGDWITMDKFGADGDVIEINLTTVKVRN
FDNTITTIPTYALSSDSFQNWRGMQKSDGRRIKRHVLIKSSSIRFLNNDDLNHMKGIQLITSYIETRQAEIEKYNELRGI
DKSLLLNGRNMTNLGMFRKYIMQYLLDHPGLNKNMHIMCRQLQSTAHGVPLEIYVFSSDKRWANYEYIMSDIFDHVMASV
IYFDLEIFELPSQIGRLDR

Specific function: Mechanosensitive channel of miniconductance that confers protection against mild hypoosmotic shock. Overexpression confers protection against severe shocks [H]

COG id: COG0668

COG function: function code M; Small-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscS (TC 1.A.23) family [H]

Homologues:

Organism=Escherichia coli, GI1786791, Length=299, Percent_Identity=42.809364548495, Blast_Score=264, Evalue=6e-72,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010920
- InterPro:   IPR011066
- InterPro:   IPR006685 [H]

Pfam domain/function: PF00924 MS_channel [H]

EC number: NA

Molecular weight: Translated: 48880; Mature: 48880

Theoretical pI: Translated: 9.53; Mature: 9.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHKLLDKIFNFLYPLFRDWGMNRNFASYISLVINIAIMLVLAYAIYYIAKFVLVTLTAVF
CHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AQRTKTKFDDYLIHNKTTKYTAYLIPFFFIYKAVPVILDKYEYWELLFGKIVGIYIVLIS
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LWIIRTIFNALRDYLKQKPEYSDKPIDSFVQVIMIVLWIFGVAMIISTLFGIKKGELLTI
HHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
LGTLSAIIILIFRDTILGFVSSVQVAINDMVRIGDWITMDKFGADGDVIEINLTTVKVRN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCEEEEEEEEEEEEC
FDNTITTIPTYALSSDSFQNWRGMQKSDGRRIKRHVLIKSSSIRFLNNDDLNHMKGIQLI
CCCCHHHCCCHHCCCCCHHHHCCCCCCCCHHHHHHHEEECCCEEEECCCCCHHHHHHHHH
TSYIETRQAEIEKYNELRGIDKSLLLNGRNMTNLGMFRKYIMQYLLDHPGLNKNMHIMCR
HHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHH
QLQSTAHGVPLEIYVFSSDKRWANYEYIMSDIFDHVMASVIYFDLEIFELPSQIGRLDR
HHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MHKLLDKIFNFLYPLFRDWGMNRNFASYISLVINIAIMLVLAYAIYYIAKFVLVTLTAVF
CHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AQRTKTKFDDYLIHNKTTKYTAYLIPFFFIYKAVPVILDKYEYWELLFGKIVGIYIVLIS
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LWIIRTIFNALRDYLKQKPEYSDKPIDSFVQVIMIVLWIFGVAMIISTLFGIKKGELLTI
HHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
LGTLSAIIILIFRDTILGFVSSVQVAINDMVRIGDWITMDKFGADGDVIEINLTTVKVRN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCEEEEEEEEEEEEC
FDNTITTIPTYALSSDSFQNWRGMQKSDGRRIKRHVLIKSSSIRFLNNDDLNHMKGIQLI
CCCCHHHCCCHHCCCCCHHHHCCCCCCCCHHHHHHHEEECCCEEEECCCCCHHHHHHHHH
TSYIETRQAEIEKYNELRGIDKSLLLNGRNMTNLGMFRKYIMQYLLDHPGLNKNMHIMCR
HHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHH
QLQSTAHGVPLEIYVFSSDKRWANYEYIMSDIFDHVMASVIYFDLEIFELPSQIGRLDR
HHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503; 7813889 [H]