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The map label for this gene is cbs [H]

Identifier: 146300312

GI number: 146300312

Start: 3041389

End: 3042429

Strand: Direct

Name: cbs [H]

Synonym: Fjoh_2557

Alternate gene names: 146300312

Gene position: 3041389-3042429 (Clockwise)

Preceding gene: 146300311

Following gene: 146300313

Centisome position: 49.88

GC content: 34.68

Gene sequence:

>1041_bases
ATGAAAGAAGAAATAAACGCTTATAATAATGTTTTAGAGTTAATAGGTAACACCCCACTTATTAAACTAAATAAAATCAC
CGAAGAGTTAGAAGGAAATTTCTACGCAAAGGTAGAAGCTTTTAATCCAGGTCATTCCTCAAAAGATAGAATAGCGTTAT
ATATTATTGAAGAAGCCGAAAGAAAAGGAATATTATCACCAGGCGACACCATAATTGAGACAACATCTGGTAATACAGGT
TTCAGCTTAGCGATGGTAAGTATAATAAAAGGGTATAATTGTATTCTGGCTGTAAGTTCAAAATCATCTAAGGACAAAAT
TGACATGCTGCGAAGTTTAGGAGCAAAAGTGTATGTCTGTCCGGCCCACGTTTCTGCAGATGATGAAAGATCTTATTATA
ATGTAGCAAAACGTTTACACGAGGAAACAAAAGGTTCAGTTTACATTAATCAATATTTCAACCAGCTAAATATTGATGCC
CACTACAACACTACAGGTCCCGAAATCTGGGAACAAACAAAAGGAAAAATTACACATCTTATTGCTTGCAGCGGAACCGG
AGGAACAATTTCAGGAACGGCGAAATTCTTAAAAGAGCAAAATCCGAATATTAGAATCTTAGGTGTTGATGCTTTTGGAT
CAGTATTGAAAAAATATCATGAAACAAGAGAGTTTGACAGCAAAGAAATTTACCCATATCGTATAGAAGGTTTAGGTAAA
AATTTAATTCCATCAGCTACAGATTTCGATATCATCGATAAATTCATGAAAGTGACCGATGAAGAAAGTGCACACTCAGC
AAGAGAAATCACTCGTAAAGAAGGTTTATTTGTTGGTTATACATCTGGAGCAGTAATGCAGGCGATTAAACAATATGCAG
AAGAAGGTGAGTTTACAAAAGACAGTAATATTATAGCGATTTTCCCTGATCATGGTTCACGCTACATGAGTAAAGTATTC
AGCGACGACTGGATGAATGAACAAGGTTTCTTTGATAGTGTTAATGAAGAAGAAGCTCAAAAAATTGAATTTGTAAAATA
A

Upstream 100 bases:

>100_bases
TTATTAGATTTAGAAAAAATTAAAAGCTAATTAGAAATTTTTTGTTAAATTTGCAACCTATCAAGGTTATTTAAAAATAC
CTTCGATTAACTATTTTTTT

Downstream 100 bases:

>100_bases
GATTTATCTTAAATATATTTAAAACTCCATTCGCCAAGAATGGAGTTTTTTTTATTAATCATATTTATTTTATTGTCTAA
ATTGTATCTTTTGTGGAGAA

Product: pyridoxal-5'-phosphate-dependent enzyme subunit beta

Products: NA

Alternate protein names: Beta-thionase; Serine sulfhydrase [H]

Number of amino acids: Translated: 346; Mature: 346

Protein sequence:

>346_residues
MKEEINAYNNVLELIGNTPLIKLNKITEELEGNFYAKVEAFNPGHSSKDRIALYIIEEAERKGILSPGDTIIETTSGNTG
FSLAMVSIIKGYNCILAVSSKSSKDKIDMLRSLGAKVYVCPAHVSADDERSYYNVAKRLHEETKGSVYINQYFNQLNIDA
HYNTTGPEIWEQTKGKITHLIACSGTGGTISGTAKFLKEQNPNIRILGVDAFGSVLKKYHETREFDSKEIYPYRIEGLGK
NLIPSATDFDIIDKFMKVTDEESAHSAREITRKEGLFVGYTSGAVMQAIKQYAEEGEFTKDSNIIAIFPDHGSRYMSKVF
SDDWMNEQGFFDSVNEEEAQKIEFVK

Sequences:

>Translated_346_residues
MKEEINAYNNVLELIGNTPLIKLNKITEELEGNFYAKVEAFNPGHSSKDRIALYIIEEAERKGILSPGDTIIETTSGNTG
FSLAMVSIIKGYNCILAVSSKSSKDKIDMLRSLGAKVYVCPAHVSADDERSYYNVAKRLHEETKGSVYINQYFNQLNIDA
HYNTTGPEIWEQTKGKITHLIACSGTGGTISGTAKFLKEQNPNIRILGVDAFGSVLKKYHETREFDSKEIYPYRIEGLGK
NLIPSATDFDIIDKFMKVTDEESAHSAREITRKEGLFVGYTSGAVMQAIKQYAEEGEFTKDSNIIAIFPDHGSRYMSKVF
SDDWMNEQGFFDSVNEEEAQKIEFVK
>Mature_346_residues
MKEEINAYNNVLELIGNTPLIKLNKITEELEGNFYAKVEAFNPGHSSKDRIALYIIEEAERKGILSPGDTIIETTSGNTG
FSLAMVSIIKGYNCILAVSSKSSKDKIDMLRSLGAKVYVCPAHVSADDERSYYNVAKRLHEETKGSVYINQYFNQLNIDA
HYNTTGPEIWEQTKGKITHLIACSGTGGTISGTAKFLKEQNPNIRILGVDAFGSVLKKYHETREFDSKEIYPYRIEGLGK
NLIPSATDFDIIDKFMKVTDEESAHSAREITRKEGLFVGYTSGAVMQAIKQYAEEGEFTKDSNIIAIFPDHGSRYMSKVF
SDDWMNEQGFFDSVNEEEAQKIEFVK

Specific function: Two Cysteine Synthase Enzymes Are Found. Both Catalyze The Same Reaction. Cysteine Synthase B Can Also Use Thiosulfate In Place Of Sulfide To Give Cysteine Thiosulfonate As A Product. [C]

COG id: COG0031

COG function: function code E; Cysteine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the cysteine synthase/cystathionine beta- synthase family [H]

Homologues:

Organism=Homo sapiens, GI295821202, Length=324, Percent_Identity=42.5925925925926, Blast_Score=268, Evalue=8e-72,
Organism=Homo sapiens, GI295821200, Length=324, Percent_Identity=42.5925925925926, Blast_Score=268, Evalue=8e-72,
Organism=Homo sapiens, GI4557415, Length=324, Percent_Identity=42.5925925925926, Blast_Score=268, Evalue=8e-72,
Organism=Escherichia coli, GI2367138, Length=312, Percent_Identity=32.3717948717949, Blast_Score=184, Evalue=9e-48,
Organism=Escherichia coli, GI1788754, Length=320, Percent_Identity=33.125, Blast_Score=169, Evalue=2e-43,
Organism=Caenorhabditis elegans, GI17534315, Length=330, Percent_Identity=37.2727272727273, Blast_Score=204, Evalue=7e-53,
Organism=Caenorhabditis elegans, GI25147552, Length=331, Percent_Identity=36.8580060422961, Blast_Score=199, Evalue=2e-51,
Organism=Caenorhabditis elegans, GI17535051, Length=331, Percent_Identity=33.5347432024169, Blast_Score=199, Evalue=2e-51,
Organism=Caenorhabditis elegans, GI115535073, Length=331, Percent_Identity=34.7432024169184, Blast_Score=190, Evalue=1e-48,
Organism=Caenorhabditis elegans, GI17562970, Length=334, Percent_Identity=33.2335329341317, Blast_Score=185, Evalue=3e-47,
Organism=Caenorhabditis elegans, GI32566674, Length=308, Percent_Identity=34.0909090909091, Blast_Score=179, Evalue=1e-45,
Organism=Caenorhabditis elegans, GI17561720, Length=333, Percent_Identity=32.1321321321321, Blast_Score=157, Evalue=5e-39,
Organism=Caenorhabditis elegans, GI32566672, Length=245, Percent_Identity=28.9795918367347, Blast_Score=110, Evalue=9e-25,
Organism=Saccharomyces cerevisiae, GI6321594, Length=339, Percent_Identity=40.117994100295, Blast_Score=259, Evalue=3e-70,
Organism=Saccharomyces cerevisiae, GI6321449, Length=333, Percent_Identity=38.1381381381381, Blast_Score=160, Evalue=2e-40,
Organism=Drosophila melanogaster, GI24643623, Length=329, Percent_Identity=39.8176291793313, Blast_Score=241, Evalue=4e-64,
Organism=Drosophila melanogaster, GI20129101, Length=329, Percent_Identity=39.8176291793313, Blast_Score=241, Evalue=4e-64,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR005857
- InterPro:   IPR000644
- InterPro:   IPR001926 [H]

Pfam domain/function: PF00571 CBS; PF00291 PALP [H]

EC number: =4.2.1.22 [H]

Molecular weight: Translated: 38804; Mature: 38804

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKEEINAYNNVLELIGNTPLIKLNKITEELEGNFYAKVEAFNPGHSSKDRIALYIIEEAE
CCCHHHHHHHHHHHHCCCCEEEEHHHHHHHCCCEEEEEEEECCCCCCCCCEEEEEEEEHH
RKGILSPGDTIIETTSGNTGFSLAMVSIIKGYNCILAVSSKSSKDKIDMLRSLGAKVYVC
HCCCCCCCCCEEEECCCCCCHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHCCCEEEEE
PAHVSADDERSYYNVAKRLHEETKGSVYINQYFNQLNIDAHYNTTGPEIWEQTKGKITHL
ECCCCCCCCHHHHHHHHHHHHHHCCCEEEEEHHHHCCCCEEECCCCHHHHHHCCCCEEEE
IACSGTGGTISGTAKFLKEQNPNIRILGVDAFGSVLKKYHETREFDSKEIYPYRIEGLGK
EEECCCCCCCHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHCCCCCCCCCEEECCCCC
NLIPSATDFDIIDKFMKVTDEESAHSAREITRKEGLFVGYTSGAVMQAIKQYAEEGEFTK
CCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHCCCCCC
DSNIIAIFPDHGSRYMSKVFSDDWMNEQGFFDSVNEEEAQKIEFVK
CCCEEEEECCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHCCCC
>Mature Secondary Structure
MKEEINAYNNVLELIGNTPLIKLNKITEELEGNFYAKVEAFNPGHSSKDRIALYIIEEAE
CCCHHHHHHHHHHHHCCCCEEEEHHHHHHHCCCEEEEEEEECCCCCCCCCEEEEEEEEHH
RKGILSPGDTIIETTSGNTGFSLAMVSIIKGYNCILAVSSKSSKDKIDMLRSLGAKVYVC
HCCCCCCCCCEEEECCCCCCHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHCCCEEEEE
PAHVSADDERSYYNVAKRLHEETKGSVYINQYFNQLNIDAHYNTTGPEIWEQTKGKITHL
ECCCCCCCCHHHHHHHHHHHHHHCCCEEEEEHHHHCCCCEEECCCCHHHHHHCCCCEEEE
IACSGTGGTISGTAKFLKEQNPNIRILGVDAFGSVLKKYHETREFDSKEIYPYRIEGLGK
EEECCCCCCCHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHCCCCCCCCCEEECCCCC
NLIPSATDFDIIDKFMKVTDEESAHSAREITRKEGLFVGYTSGAVMQAIKQYAEEGEFTK
CCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHCCCCCC
DSNIIAIFPDHGSRYMSKVFSDDWMNEQGFFDSVNEEEAQKIEFVK
CCCEEEEECCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]