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The map label for this gene is ltaA [H]

Identifier: 146300309

GI number: 146300309

Start: 3037634

End: 3038653

Strand: Direct

Name: ltaA [H]

Synonym: Fjoh_2554

Alternate gene names: 146300309

Gene position: 3037634-3038653 (Clockwise)

Preceding gene: 146300305

Following gene: 146300311

Centisome position: 49.82

GC content: 37.35

Gene sequence:

>1020_bases
ATGGAAATAAATTTAATCAGCGATACCATTACTAAGCCTACGTATGAGATGCTTCAGTATATGTTTAACGCTCACGTTGG
CGACGATGTATACAAGCAAGATCCTACGGTTATCGAATTAGAGACAAGAGTAGCCGAGTTTTTTGGTATGGAAGCTGGAC
TTTTTTTTCCGTCTGGAACTATGGCAAATCAAACTGCAATTAAACTTCATACACAGCCGGGTGAACAGTTAATTGCTGAT
AAATATGCTCATGTTTTTCATTATGAAGGAGGAGGAGTTTCTTTTAACAGTGGTGTTTCATGCTGTTTGTTAGATGGAGA
CCGCGGTATGATAAATGCAAAACAGGTTGCTGCAGCGATAAACGATCCGGAATTTTACCATAGTCCGCTGACGAGTTTGG
TTTGTGTAGAAAACACAACAAACAAAGGCGGAGGTGCTTGTTATGAATTGCAGGATTTAAGAGATATTAAAAAAGTTTGC
GACGCTAATAATTTGAAGTTCCACTTAGACGGTGCCAGAATTTGGAATGCATTGGTTGCTAAAAGACAAAACCCGAAGGA
ATTTGGAGCTATTTTCGATACTATTTCAGTTTGTTTGTCTAAAGGATTAGGAGCTCCAATTGGTTCTGTTTTGCTTGGAA
GTAAAGCTGATATTCATAGAGCGTTGAGAATCAGAAAGATATTAGGTGGTGGAATGCGTCAGGTAGGTTATTTGGCTGCA
GCGGGATTATATGCTTTGGCAAACAATATTGAACGTCTTGCCGAAGATCATCGCAGAGCAAAAGAAATTGGAGCAATTTT
AAGCACCAAATCTTGGGTGGCTTCAGTAGAACCAGTTGAAACAAATATTTTGATTTTTTCATTAGCAGAAGGCTACAGCG
ACCAGCTTTTGATAGAAAAATTAAAACAAAAAAATATTTTAATAAGCTCTTTAGGTCATAATAAACTTAGAATCGTTACG
CATTTAGATTATAAAGAGGTAATGCATACTTATGTAGTTGAAACGCTTTCGAAGATTTAG

Upstream 100 bases:

>100_bases
AATTTGGTTCTATGTAATCTCTAAGTTAAAATTTATTAAAACTTGAGATTATTAACAAACAGTTATACGTAATTTTGCAG
TCCAAAATATATAGTTTTAT

Downstream 100 bases:

>100_bases
GAAAGAGGGACAAAGGTTTAGAGGGGCAAAGGTTAAAAAAAAAGACGCATTATTTGCGTCTTTTTTTTGATTCAAGGTTT
TCATAATAAAAAACCTTTGT

Product: threonine aldolase

Products: glycine; acetaldehyde

Alternate protein names: L-allo-TA; L-allo-threonine acetaldehyde-lyase [H]

Number of amino acids: Translated: 339; Mature: 339

Protein sequence:

>339_residues
MEINLISDTITKPTYEMLQYMFNAHVGDDVYKQDPTVIELETRVAEFFGMEAGLFFPSGTMANQTAIKLHTQPGEQLIAD
KYAHVFHYEGGGVSFNSGVSCCLLDGDRGMINAKQVAAAINDPEFYHSPLTSLVCVENTTNKGGGACYELQDLRDIKKVC
DANNLKFHLDGARIWNALVAKRQNPKEFGAIFDTISVCLSKGLGAPIGSVLLGSKADIHRALRIRKILGGGMRQVGYLAA
AGLYALANNIERLAEDHRRAKEIGAILSTKSWVASVEPVETNILIFSLAEGYSDQLLIEKLKQKNILISSLGHNKLRIVT
HLDYKEVMHTYVVETLSKI

Sequences:

>Translated_339_residues
MEINLISDTITKPTYEMLQYMFNAHVGDDVYKQDPTVIELETRVAEFFGMEAGLFFPSGTMANQTAIKLHTQPGEQLIAD
KYAHVFHYEGGGVSFNSGVSCCLLDGDRGMINAKQVAAAINDPEFYHSPLTSLVCVENTTNKGGGACYELQDLRDIKKVC
DANNLKFHLDGARIWNALVAKRQNPKEFGAIFDTISVCLSKGLGAPIGSVLLGSKADIHRALRIRKILGGGMRQVGYLAA
AGLYALANNIERLAEDHRRAKEIGAILSTKSWVASVEPVETNILIFSLAEGYSDQLLIEKLKQKNILISSLGHNKLRIVT
HLDYKEVMHTYVVETLSKI
>Mature_339_residues
MEINLISDTITKPTYEMLQYMFNAHVGDDVYKQDPTVIELETRVAEFFGMEAGLFFPSGTMANQTAIKLHTQPGEQLIAD
KYAHVFHYEGGGVSFNSGVSCCLLDGDRGMINAKQVAAAINDPEFYHSPLTSLVCVENTTNKGGGACYELQDLRDIKKVC
DANNLKFHLDGARIWNALVAKRQNPKEFGAIFDTISVCLSKGLGAPIGSVLLGSKADIHRALRIRKILGGGMRQVGYLAA
AGLYALANNIERLAEDHRRAKEIGAILSTKSWVASVEPVETNILIFSLAEGYSDQLLIEKLKQKNILISSLGHNKLRIVT
HLDYKEVMHTYVVETLSKI

Specific function: Stereospecifically catalyzes the interconversion of L- allo-threonine and glycine [H]

COG id: COG2008

COG function: function code E; Threonine aldolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the threonine aldolase family [H]

Homologues:

Organism=Escherichia coli, GI1787095, Length=322, Percent_Identity=41.304347826087, Blast_Score=217, Evalue=8e-58,
Organism=Caenorhabditis elegans, GI193206520, Length=343, Percent_Identity=37.3177842565598, Blast_Score=213, Evalue=1e-55,
Organism=Caenorhabditis elegans, GI193206524, Length=344, Percent_Identity=37.2093023255814, Blast_Score=213, Evalue=1e-55,
Organism=Caenorhabditis elegans, GI193206522, Length=343, Percent_Identity=37.3177842565598, Blast_Score=213, Evalue=1e-55,
Organism=Saccharomyces cerevisiae, GI6320789, Length=309, Percent_Identity=37.5404530744337, Blast_Score=183, Evalue=4e-47,
Organism=Drosophila melanogaster, GI21355439, Length=300, Percent_Identity=43.6666666666667, Blast_Score=214, Evalue=6e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001597
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01212 Beta_elim_lyase [H]

EC number: 4.1.2.5

Molecular weight: Translated: 37333; Mature: 37333

Theoretical pI: Translated: 6.90; Mature: 6.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEINLISDTITKPTYEMLQYMFNAHVGDDVYKQDPTVIELETRVAEFFGMEAGLFFPSGT
CEEEEECHHHCCCHHHHHHHHHHCCCCCHHHCCCCCEEEHHHHHHHHHCCCCCEECCCCC
MANQTAIKLHTQPGEQLIADKYAHVFHYEGGGVSFNSGVSCCLLDGDRGMINAKQVAAAI
CCCCEEEEEECCCCHHHHHHHHHHEEEECCCCEEECCCCEEEEEECCCCCCCHHHHHHHC
NDPEFYHSPLTSLVCVENTTNKGGGACYELQDLRDIKKVCDANNLKFHLDGARIWNALVA
CCCHHHHCCHHHEEEEECCCCCCCCEEEEHHHHHHHHHHHCCCCCEEEECHHHHHHHHHH
KRQNPKEFGAIFDTISVCLSKGLGAPIGSVLLGSKADIHRALRIRKILGGGMRQVGYLAA
CCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCHHHHHHHHHHHHHHCCCHHHHHHHHH
AGLYALANNIERLAEDHRRAKEIGAILSTKSWVASVEPVETNILIFSLAEGYSDQLLIEK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHCCCCCCCCEEEEEEECCCCCHHHHHHH
LKQKNILISSLGHNKLRIVTHLDYKEVMHTYVVETLSKI
HHHCCEEEEECCCCEEEEEEECCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MEINLISDTITKPTYEMLQYMFNAHVGDDVYKQDPTVIELETRVAEFFGMEAGLFFPSGT
CEEEEECHHHCCCHHHHHHHHHHCCCCCHHHCCCCCEEEHHHHHHHHHCCCCCEECCCCC
MANQTAIKLHTQPGEQLIADKYAHVFHYEGGGVSFNSGVSCCLLDGDRGMINAKQVAAAI
CCCCEEEEEECCCCHHHHHHHHHHEEEECCCCEEECCCCEEEEEECCCCCCCHHHHHHHC
NDPEFYHSPLTSLVCVENTTNKGGGACYELQDLRDIKKVCDANNLKFHLDGARIWNALVA
CCCHHHHCCHHHEEEEECCCCCCCCEEEEHHHHHHHHHHHCCCCCEEEECHHHHHHHHHH
KRQNPKEFGAIFDTISVCLSKGLGAPIGSVLLGSKADIHRALRIRKILGGGMRQVGYLAA
CCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCHHHHHHHHHHHHHHCCCHHHHHHHHH
AGLYALANNIERLAEDHRRAKEIGAILSTKSWVASVEPVETNILIFSLAEGYSDQLLIEK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHCCCCCCCCEEEEEEECCCCCHHHHHHH
LKQKNILISSLGHNKLRIVTHLDYKEVMHTYVVETLSKI
HHHCCEEEEECCCCEEEEEEECCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Pyridoxal phosphate [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: 1.7

Km value (mM): 2.85 {L-Thr}} 0.24 {DL-erythro-phenylserine}} 0.22 {L-allo-Thr}} 0.12 {DL-threo-phenylserine}} [C]

Substrates: L-threonine

Specific reaction: L-threonine = glycine + acetaldehyde

General reaction: Elimination of an aldehyde, C-C bond cleavage [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9171400; 9228760 [H]