Click here to switch to the map view.

The map label for this gene is iscS [H]

Identifier: 146300266

GI number: 146300266

Start: 2996117

End: 2997286

Strand: Direct

Name: iscS [H]

Synonym: Fjoh_2511

Alternate gene names: 146300266

Gene position: 2996117-2997286 (Clockwise)

Preceding gene: 146300264

Following gene: 146300268

Centisome position: 49.14

GC content: 32.05

Gene sequence:

>1170_bases
ATGATATTTAAGATTAGACAATTGATATTTTATAACTTTGCATTAATGAAAAAAGTATATCTCGATAACGCCTCAACTAC
TGCGATGCGTCCAGAAGTTGTTCAGGAAATGACTAAAATTATGAGTGAAGATTTTGGAAATCCATCATCTACACATAGTT
TTGGACGTAATGCAAAAACAATTTTAGAACTTTCAAGAAAAACTATTGCTAAATATTTAAATTGTTCTGCACAGGAAATT
CTTTTTACTTCGGGCGGGACTGAAGCTGATAACTGGATTTTAAGATCGGCAGTTGAAGATCTTAAGGTAGAACGTATTAT
TACTTCAAGAATTGAACATCATGCCGTTTTATACACTGTTTTGGCTTTACAGTCTGATTATAATATTCAGGTTGATTATG
TAAATGTAAATACTGACGGAAGCATTGATTTAACGCATCTGTCTAATTTATTATCTGACGAAAAGAAGACAATCGTTAGT
TTAATGCATGTAAACAACGAAACAGGAACTATTTTAGATTTAGAAAGAGTAAGTGTTATTTGCAAGCAATATAATGCCTT
ATTTCATTCAGATACAGTTCAGTCAGTTGGTAAAACAGAAATTGATCTTCAAAAAACTTCTGTAGACTTTATTTTAGCAA
GCGCACATAAATTTCATGGACCAAAAGGTATTGGTTTTGCTTTTATTCGAAAAAATACTGGTTTACAGCCTTTAATTTTT
GGAGGTGAGCAGGAAAAAGGATTGCGTGCTGGGACAGAAGCAGTGCATCAAATTGCCGGAATGGCTAAAGCGCTGTCTCT
TTCTTATGAAAATTTAGATCAGGAGAGAAATTATATTTCTGATTTAAAGAAATATCTAATACAACAATTAGAAATTGAAT
TTCCGGGTTTTAGAATAAACGGGAAAAAGGATGATTTTTATAATATTATAAATATTACACTGCCATTTTCTTCAGATAAA
ACTTCTATGCTTTTGTTTAGTTTGGATATGAAGGGGATTGCTGTTTCTAGAGGAAGTGCCTGTCAGTCAGGAAGTATAAA
GCCTTCGCATGTTTTAAAAGAAATGCTTTCAGAAGCTGACTTAAAACTGCCAAATCTCAGAATTTCATTTAGTCATTACA
ATACAAGAGAAGATATCGACTGGCTGATTGAGAGTTTAAAAAATGTTTAA

Upstream 100 bases:

>100_bases
CCATCGTCGCAGCCGTTTAACAAAAACGCCAGAAATATAAGGCTTGCATATTTTTTCATTGTAGTTTTATTTGTGCCAAA
AATAGTGAAAAATTTGGCAA

Downstream 100 bases:

>100_bases
AAAGATGTACAACTCTGAGTTTGCATTTTTTAAAATAATAAAAAGCCGGATCAAAAAACAATTTGATCCGGCTTTTTAAT
TTAAGTTCTATTTTCGAATA

Product: class V aminotransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 389; Mature: 389

Protein sequence:

>389_residues
MIFKIRQLIFYNFALMKKVYLDNASTTAMRPEVVQEMTKIMSEDFGNPSSTHSFGRNAKTILELSRKTIAKYLNCSAQEI
LFTSGGTEADNWILRSAVEDLKVERIITSRIEHHAVLYTVLALQSDYNIQVDYVNVNTDGSIDLTHLSNLLSDEKKTIVS
LMHVNNETGTILDLERVSVICKQYNALFHSDTVQSVGKTEIDLQKTSVDFILASAHKFHGPKGIGFAFIRKNTGLQPLIF
GGEQEKGLRAGTEAVHQIAGMAKALSLSYENLDQERNYISDLKKYLIQQLEIEFPGFRINGKKDDFYNIINITLPFSSDK
TSMLLFSLDMKGIAVSRGSACQSGSIKPSHVLKEMLSEADLKLPNLRISFSHYNTREDIDWLIESLKNV

Sequences:

>Translated_389_residues
MIFKIRQLIFYNFALMKKVYLDNASTTAMRPEVVQEMTKIMSEDFGNPSSTHSFGRNAKTILELSRKTIAKYLNCSAQEI
LFTSGGTEADNWILRSAVEDLKVERIITSRIEHHAVLYTVLALQSDYNIQVDYVNVNTDGSIDLTHLSNLLSDEKKTIVS
LMHVNNETGTILDLERVSVICKQYNALFHSDTVQSVGKTEIDLQKTSVDFILASAHKFHGPKGIGFAFIRKNTGLQPLIF
GGEQEKGLRAGTEAVHQIAGMAKALSLSYENLDQERNYISDLKKYLIQQLEIEFPGFRINGKKDDFYNIINITLPFSSDK
TSMLLFSLDMKGIAVSRGSACQSGSIKPSHVLKEMLSEADLKLPNLRISFSHYNTREDIDWLIESLKNV
>Mature_389_residues
MIFKIRQLIFYNFALMKKVYLDNASTTAMRPEVVQEMTKIMSEDFGNPSSTHSFGRNAKTILELSRKTIAKYLNCSAQEI
LFTSGGTEADNWILRSAVEDLKVERIITSRIEHHAVLYTVLALQSDYNIQVDYVNVNTDGSIDLTHLSNLLSDEKKTIVS
LMHVNNETGTILDLERVSVICKQYNALFHSDTVQSVGKTEIDLQKTSVDFILASAHKFHGPKGIGFAFIRKNTGLQPLIF
GGEQEKGLRAGTEAVHQIAGMAKALSLSYENLDQERNYISDLKKYLIQQLEIEFPGFRINGKKDDFYNIINITLPFSSDK
TSMLLFSLDMKGIAVSRGSACQSGSIKPSHVLKEMLSEADLKLPNLRISFSHYNTREDIDWLIESLKNV

Specific function: Catalyzes the removal of elemental sulfur from cysteine to produce alanine [H]

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=376, Percent_Identity=31.1170212765957, Blast_Score=213, Evalue=2e-55,
Organism=Homo sapiens, GI156713448, Length=414, Percent_Identity=31.6425120772947, Blast_Score=196, Evalue=3e-50,
Organism=Escherichia coli, GI48994898, Length=377, Percent_Identity=35.5437665782493, Blast_Score=229, Evalue=3e-61,
Organism=Escherichia coli, GI1787970, Length=420, Percent_Identity=25.7142857142857, Blast_Score=108, Evalue=5e-25,
Organism=Escherichia coli, GI1789175, Length=396, Percent_Identity=26.7676767676768, Blast_Score=100, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI25143064, Length=375, Percent_Identity=32.2666666666667, Blast_Score=209, Evalue=3e-54,
Organism=Caenorhabditis elegans, GI17533177, Length=336, Percent_Identity=28.2738095238095, Blast_Score=117, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6319831, Length=378, Percent_Identity=34.1269841269841, Blast_Score=227, Evalue=3e-60,
Organism=Drosophila melanogaster, GI20129463, Length=370, Percent_Identity=32.4324324324324, Blast_Score=217, Evalue=9e-57,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR010240
- InterPro:   IPR017772
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =2.8.1.7 [H]

Molecular weight: Translated: 43804; Mature: 43804

Theoretical pI: Translated: 6.92; Mature: 6.92

Prosite motif: PS00595 AA_TRANSFER_CLASS_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIFKIRQLIFYNFALMKKVYLDNASTTAMRPEVVQEMTKIMSEDFGNPSSTHSFGRNAKT
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHCCCCHHH
ILELSRKTIAKYLNCSAQEILFTSGGTEADNWILRSAVEDLKVERIITSRIEHHAVLYTV
HHHHHHHHHHHHHCCCHHHEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LALQSDYNIQVDYVNVNTDGSIDLTHLSNLLSDEKKTIVSLMHVNNETGTILDLERVSVI
HHHCCCCEEEEEEEEECCCCCEEHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEHHHHHHH
CKQYNALFHSDTVQSVGKTEIDLQKTSVDFILASAHKFHGPKGIGFAFIRKNTGLQPLIF
HHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHEEHHHHCCCCCCCEEEEEECCCCCCEEEE
GGEQEKGLRAGTEAVHQIAGMAKALSLSYENLDQERNYISDLKKYLIQQLEIEFPGFRIN
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEC
GKKDDFYNIINITLPFSSDKTSMLLFSLDMKGIAVSRGSACQSGSIKPSHVLKEMLSEAD
CCCCCCEEEEEEEEECCCCCCEEEEEEECCCCEEEECCCCCCCCCCCHHHHHHHHHHHCC
LKLPNLRISFSHYNTREDIDWLIESLKNV
CCCCCEEEEECCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MIFKIRQLIFYNFALMKKVYLDNASTTAMRPEVVQEMTKIMSEDFGNPSSTHSFGRNAKT
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHCCCCHHH
ILELSRKTIAKYLNCSAQEILFTSGGTEADNWILRSAVEDLKVERIITSRIEHHAVLYTV
HHHHHHHHHHHHHCCCHHHEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LALQSDYNIQVDYVNVNTDGSIDLTHLSNLLSDEKKTIVSLMHVNNETGTILDLERVSVI
HHHCCCCEEEEEEEEECCCCCEEHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEHHHHHHH
CKQYNALFHSDTVQSVGKTEIDLQKTSVDFILASAHKFHGPKGIGFAFIRKNTGLQPLIF
HHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHEEHHHHCCCCCCCEEEEEECCCCCCEEEE
GGEQEKGLRAGTEAVHQIAGMAKALSLSYENLDQERNYISDLKKYLIQQLEIEFPGFRIN
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEC
GKKDDFYNIINITLPFSSDKTSMLLFSLDMKGIAVSRGSACQSGSIKPSHVLKEMLSEAD
CCCCCCEEEEEEEEECCCCCCEEEEEEECCCCEEEECCCCCCCCCCCHHHHHHHHHHHCC
LKLPNLRISFSHYNTREDIDWLIESLKNV
CCCCCEEEEECCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA