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The map label for this gene is guaA

Identifier: 146300132

GI number: 146300132

Start: 2846242

End: 2847771

Strand: Direct

Name: guaA

Synonym: Fjoh_2377

Alternate gene names: 146300132

Gene position: 2846242-2847771 (Clockwise)

Preceding gene: 146300130

Following gene: 146300133

Centisome position: 46.68

GC content: 37.45

Gene sequence:

>1530_bases
ATGCAACACAACGTACTTATTTTAGATTTCGGATCGCAATATACTCAGCTTATTGCGCGTAGAGTTCGCGAATTAAATAT
ATTCTGCGAAATTTTTCCTTACAATCACATTCCAAGTGATTTATCAAGTTATAAAGCCGTAATTTTAGGAGGAAGCCCTT
TCTCTGTTAGAGGAGAAGATGCGCCGCATCCTGATTTATCACAAATTAGAGGTAAAATGCCTTTGCTTGCTGTTTGTTAC
GGAGCTCAATATTTAGCTCATTTCAGCGGTGGAGAAGTGGCAGCTTCTAATACAAGAGAATACGGAAGAGCAAATTTGTC
TTATATTAAAGAAGGCGAGACTTTCTTCGAAGGAGTTTCAGAAAACAGCCAGGTTTGGATGAGCCACAGCGATAGTATCA
AAGCGCTTCCAACAAATGCTGTAAAATTAGCAAGCACGCATGACGTAGAATACGCAGCTTATAAAATTGAAGGCGAAACT
ACTTATGCTATTCAATATCACCCAGAAGTTTACCATTCAACAGATGGTAAAAAAATGCTGGAAAACTTCTTGGTAAAAAT
TGCTGAAGTTCCTCAAAACTTTACTCCAAATGCTTTCGTTGACGAAATGGTGGCAGAATTAAAAGAAAAATTAGGAAACG
ATAAAGTGGTTCTTGGTTTATCTGGAGGAGTAGATTCTACTGTAGCAGCAGTTTTATTAAACAAAGCAATCGGACAAAAT
TTATATTGCATCTTCGTTAACAACGGACTTTTACGTAAAAACGAATTCCAAAATGTATTAGATCAATACAAAGGAATGGG
ATTAAATGTAAAAGGTGTAGATGCCGGAGATCGTTTCCTTGGCGAATTAGCAGGAATCAGCGATCCAGAAACAAAACGTA
AAACAATTGGTCGTGTATTTATCGAAGTTTTTGATGATGAATCACACTTATTAGAAGACGTAAAATGGTTAGCGCAGGGA
ACAATTTACCCAGACGTAATTGAGTCTGTTTCGGTAAAAGGACCATCAGCAACTATTAAATCGCACCACAATGTGGGTGG
ATTGCCGGATTATATGAAATTAAAAATTGTAGAACCGCTTAGAATGTTGTTTAAAGACGAAGTTCGTAGAGTTGGAGCTA
CATTAGGAATAGATCCTGAATTATTAGGAAGACACCCTTTCCCAGGACCAGGATTATCAATTAGAATTTTAGGAGATATT
ACGCCGGAGAAAGTACAAATTTTACAAGATGTCGATTCTGTATTTATAGAAGGATTAAAATCTTGGGGATTATATGACAA
AGTTTGGCAGGCGGGAGCCATTTTGCTTCCAGTAAACAGTGTTGGAGTTATGGGCGATGAGCGTACTTACGAAAAAGTAG
TAGCGCTTAGAGCAGTAGAATCAACAGATGGTATGACTGCTGACTGGGTTCACTTACCGTATGATTTCTTGATGAAAGTA
TCAAATGATATTATCAATAAGGTAAAAGGCGTGAATCGTGTAGTTTACGATATTAGTTCAAAACCACCTGCAACAATTGA
GTGGGAATAG

Upstream 100 bases:

>100_bases
TCTCTAAGCCTTTGAACCTAAAAATTGGAACAATGAAAAAAAAATATTTTTTTTCATTGTTCCAATTTTTCAATTTCGAA
TATAAAAAGTATTTTTGCGC

Downstream 100 bases:

>100_bases
CAGCGTATTTTAAAATTAAGGTCTTACATTTGTAAGGCCTTAATTTTTTATAACCTACTATTTATGAGAGAACTTTTAAC
GATTTCTCTTGTGTTTGTTT

Product: GMP synthase

Products: NA

Alternate protein names: GMP synthetase; Glutamine amidotransferase

Number of amino acids: Translated: 509; Mature: 509

Protein sequence:

>509_residues
MQHNVLILDFGSQYTQLIARRVRELNIFCEIFPYNHIPSDLSSYKAVILGGSPFSVRGEDAPHPDLSQIRGKMPLLAVCY
GAQYLAHFSGGEVAASNTREYGRANLSYIKEGETFFEGVSENSQVWMSHSDSIKALPTNAVKLASTHDVEYAAYKIEGET
TYAIQYHPEVYHSTDGKKMLENFLVKIAEVPQNFTPNAFVDEMVAELKEKLGNDKVVLGLSGGVDSTVAAVLLNKAIGQN
LYCIFVNNGLLRKNEFQNVLDQYKGMGLNVKGVDAGDRFLGELAGISDPETKRKTIGRVFIEVFDDESHLLEDVKWLAQG
TIYPDVIESVSVKGPSATIKSHHNVGGLPDYMKLKIVEPLRMLFKDEVRRVGATLGIDPELLGRHPFPGPGLSIRILGDI
TPEKVQILQDVDSVFIEGLKSWGLYDKVWQAGAILLPVNSVGVMGDERTYEKVVALRAVESTDGMTADWVHLPYDFLMKV
SNDIINKVKGVNRVVYDISSKPPATIEWE

Sequences:

>Translated_509_residues
MQHNVLILDFGSQYTQLIARRVRELNIFCEIFPYNHIPSDLSSYKAVILGGSPFSVRGEDAPHPDLSQIRGKMPLLAVCY
GAQYLAHFSGGEVAASNTREYGRANLSYIKEGETFFEGVSENSQVWMSHSDSIKALPTNAVKLASTHDVEYAAYKIEGET
TYAIQYHPEVYHSTDGKKMLENFLVKIAEVPQNFTPNAFVDEMVAELKEKLGNDKVVLGLSGGVDSTVAAVLLNKAIGQN
LYCIFVNNGLLRKNEFQNVLDQYKGMGLNVKGVDAGDRFLGELAGISDPETKRKTIGRVFIEVFDDESHLLEDVKWLAQG
TIYPDVIESVSVKGPSATIKSHHNVGGLPDYMKLKIVEPLRMLFKDEVRRVGATLGIDPELLGRHPFPGPGLSIRILGDI
TPEKVQILQDVDSVFIEGLKSWGLYDKVWQAGAILLPVNSVGVMGDERTYEKVVALRAVESTDGMTADWVHLPYDFLMKV
SNDIINKVKGVNRVVYDISSKPPATIEWE
>Mature_509_residues
MQHNVLILDFGSQYTQLIARRVRELNIFCEIFPYNHIPSDLSSYKAVILGGSPFSVRGEDAPHPDLSQIRGKMPLLAVCY
GAQYLAHFSGGEVAASNTREYGRANLSYIKEGETFFEGVSENSQVWMSHSDSIKALPTNAVKLASTHDVEYAAYKIEGET
TYAIQYHPEVYHSTDGKKMLENFLVKIAEVPQNFTPNAFVDEMVAELKEKLGNDKVVLGLSGGVDSTVAAVLLNKAIGQN
LYCIFVNNGLLRKNEFQNVLDQYKGMGLNVKGVDAGDRFLGELAGISDPETKRKTIGRVFIEVFDDESHLLEDVKWLAQG
TIYPDVIESVSVKGPSATIKSHHNVGGLPDYMKLKIVEPLRMLFKDEVRRVGATLGIDPELLGRHPFPGPGLSIRILGDI
TPEKVQILQDVDSVFIEGLKSWGLYDKVWQAGAILLPVNSVGVMGDERTYEKVVALRAVESTDGMTADWVHLPYDFLMKV
SNDIINKVKGVNRVVYDISSKPPATIEWE

Specific function: Catalyzes the synthesis of GMP from XMP

COG id: COG0519

COG function: function code F; GMP synthase, PP-ATPase domain/subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GMP-binding domain

Homologues:

Organism=Homo sapiens, GI4504035, Length=547, Percent_Identity=33.8208409506399, Blast_Score=265, Evalue=8e-71,
Organism=Escherichia coli, GI1788854, Length=520, Percent_Identity=52.3076923076923, Blast_Score=546, Evalue=1e-157,
Organism=Caenorhabditis elegans, GI71992717, Length=567, Percent_Identity=34.7442680776014, Blast_Score=273, Evalue=2e-73,
Organism=Caenorhabditis elegans, GI71992710, Length=567, Percent_Identity=34.7442680776014, Blast_Score=273, Evalue=2e-73,
Organism=Caenorhabditis elegans, GI133901714, Length=567, Percent_Identity=34.7442680776014, Blast_Score=272, Evalue=2e-73,
Organism=Saccharomyces cerevisiae, GI6323873, Length=517, Percent_Identity=50.0967117988395, Blast_Score=519, Evalue=1e-148,
Organism=Drosophila melanogaster, GI281365319, Length=553, Percent_Identity=35.4430379746835, Blast_Score=268, Evalue=7e-72,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GUAA_FLAJ1 (A5FHB4)

Other databases:

- EMBL:   CP000685
- RefSeq:   YP_001194723.1
- ProteinModelPortal:   A5FHB4
- SMR:   A5FHB4
- STRING:   A5FHB4
- GeneID:   5092109
- GenomeReviews:   CP000685_GR
- KEGG:   fjo:Fjoh_2377
- eggNOG:   COG0519
- HOGENOM:   HBG391775
- OMA:   MKLVEPL
- ProtClustDB:   PRK00074
- BioCyc:   FJOH376686:FJOH_2377-MONOMER
- HAMAP:   MF_00344
- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR001674
- InterPro:   IPR004739
- InterPro:   IPR022955
- InterPro:   IPR022310
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- TIGRFAMs:   TIGR00884
- TIGRFAMs:   TIGR00888

Pfam domain/function: PF00117 GATase; PF00958 GMP_synt_C; PF02540 NAD_synthase

EC number: =6.3.5.2

Molecular weight: Translated: 56397; Mature: 56397

Theoretical pI: Translated: 5.36; Mature: 5.36

Prosite motif: PS51273 GATASE_TYPE_1

Important sites: ACT_SITE 79-79 ACT_SITE 167-167 ACT_SITE 169-169

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQHNVLILDFGSQYTQLIARRVRELNIFCEIFPYNHIPSDLSSYKAVILGGSPFSVRGED
CCCCEEEEECCCHHHHHHHHHHHHHEEEEEEECCCCCCCCHHCCEEEEECCCCCEECCCC
APHPDLSQIRGKMPLLAVCYGAQYLAHFSGGEVAASNTREYGRANLSYIKEGETFFEGVS
CCCCCHHHHCCCCCHHHHHHHHHHHHHCCCCCEECCCCHHHCCCCHHHHHCCHHHHHCCC
ENSQVWMSHSDSIKALPTNAVKLASTHDVEYAAYKIEGETTYAIQYHPEVYHSTDGKKML
CCCEEEEECCCCCEECCCCCEEEECCCCCEEEEEEECCCEEEEEEECCHHEECCCHHHHH
ENFLVKIAEVPQNFTPNAFVDEMVAELKEKLGNDKVVLGLSGGVDSTVAAVLLNKAIGQN
HHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHCCC
LYCIFVNNGLLRKNEFQNVLDQYKGMGLNVKGVDAGDRFLGELAGISDPETKRKTIGRVF
EEEEEECCCCCCHHHHHHHHHHHCCCCCEEECCCCHHHHHHHHCCCCCCHHHHHHHHHHH
IEVFDDESHLLEDVKWLAQGTIYPDVIESVSVKGPSATIKSHHNVGGLPDYMKLKIVEPL
EEEECCHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCCHHCCCCCCCCCCCHHHEEHHHHH
RMLFKDEVRRVGATLGIDPELLGRHPFPGPGLSIRILGDITPEKVQILQDVDSVFIEGLK
HHHHHHHHHHHCCCCCCCHHHHCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHH
SWGLYDKVWQAGAILLPVNSVGVMGDERTYEKVVALRAVESTDGMTADWVHLPYDFLMKV
HCCHHHHHHHCCEEEEEECCCCCCCCCHHHHHHHHHHHECCCCCCCCCEEECCHHHHHHH
SNDIINKVKGVNRVVYDISSKPPATIEWE
HHHHHHHHHHCCEEEEEECCCCCCEEECC
>Mature Secondary Structure
MQHNVLILDFGSQYTQLIARRVRELNIFCEIFPYNHIPSDLSSYKAVILGGSPFSVRGED
CCCCEEEEECCCHHHHHHHHHHHHHEEEEEEECCCCCCCCHHCCEEEEECCCCCEECCCC
APHPDLSQIRGKMPLLAVCYGAQYLAHFSGGEVAASNTREYGRANLSYIKEGETFFEGVS
CCCCCHHHHCCCCCHHHHHHHHHHHHHCCCCCEECCCCHHHCCCCHHHHHCCHHHHHCCC
ENSQVWMSHSDSIKALPTNAVKLASTHDVEYAAYKIEGETTYAIQYHPEVYHSTDGKKML
CCCEEEEECCCCCEECCCCCEEEECCCCCEEEEEEECCCEEEEEEECCHHEECCCHHHHH
ENFLVKIAEVPQNFTPNAFVDEMVAELKEKLGNDKVVLGLSGGVDSTVAAVLLNKAIGQN
HHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHCCC
LYCIFVNNGLLRKNEFQNVLDQYKGMGLNVKGVDAGDRFLGELAGISDPETKRKTIGRVF
EEEEEECCCCCCHHHHHHHHHHHCCCCCEEECCCCHHHHHHHHCCCCCCHHHHHHHHHHH
IEVFDDESHLLEDVKWLAQGTIYPDVIESVSVKGPSATIKSHHNVGGLPDYMKLKIVEPL
EEEECCHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCCHHCCCCCCCCCCCHHHEEHHHHH
RMLFKDEVRRVGATLGIDPELLGRHPFPGPGLSIRILGDITPEKVQILQDVDSVFIEGLK
HHHHHHHHHHHCCCCCCCHHHHCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHH
SWGLYDKVWQAGAILLPVNSVGVMGDERTYEKVVALRAVESTDGMTADWVHLPYDFLMKV
HCCHHHHHHHCCEEEEEECCCCCCCCCHHHHHHHHHHHECCCCCCCCCEEECCHHHHHHH
SNDIINKVKGVNRVVYDISSKPPATIEWE
HHHHHHHHHHCCEEEEEECCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA