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The map label for this gene is ispD [H]

Identifier: 146299995

GI number: 146299995

Start: 2694348

End: 2695703

Strand: Direct

Name: ispD [H]

Synonym: Fjoh_2239

Alternate gene names: 146299995

Gene position: 2694348-2695703 (Clockwise)

Preceding gene: 146299994

Following gene: 146299996

Centisome position: 44.19

GC content: 33.41

Gene sequence:

>1356_bases
ATGAACATAGCTGTCATATTAGCAGGAGGAATAGGAATACGATTAGAGAAATCGCTGCCTAAGCAATTTTTTAAAGTTGC
CGGTAAGATGATTATTGAACATGCTGTTGATGCATTTGAAAAAAATGAATTTATTGATGAGACAGCCATTGTAATTAATA
AACATTATCTTTTTATGGCAGAAGATATGATTATTAAAAATGAGTGGAAAAAAGTAAAACGAGTTTTAATAGGGGGCGAA
GAACGATATCAGTCAAGTCTTGCTGCTATAAAATCTTATGATGAATTTAAAGATGCTAATTTAATTTTTCACGATGCGGC
CCGTCCTTTAATAAGCCAGCGGATTATTAATGACGTTGTAAAAGCTTTGAGCCATTATAAAGCAGTTGATGTTGCTATAA
ATTCAGCAGATACTATTATTGAAGTTGAAAATGATATTATAACAGCTATTCCTGAACGAATAAAAATGCGAAGAGGACAA
ACTCCTCAAGGTTTTAAACAGGAAATTATCGCAAAAGCCTATGAGTTCGCATTAAAAGATGAAAATTTTAAAGCGACTGA
TGATTGCGGCGTGGTTAAAAAATATCTGCCTCAGGAAGATATTTATGTAGTAAACGGAGAAGAGGTTAATATGAAATTGA
CTTATCCTGAAGATACTTATTTATTAGATAAACTATTTCAATTGCGATCGGTTGAGATTCAGGATATAAAATTATCAAAG
GAAATTCTGTCTGATAAAGTTATGGTTGTTTTTGGCGGTTCTTACGGAATAGGAAAAAGTATTGTAGACCTGGCAGAACA
AAATGGAGTTAAGGTTTATAGTTTTTCCCGAAGCGAAAATAATGTTGATGTTTCTAATATTGAATTAGTCAAAAAAGCTT
TAGAAAAAGTTTTTGAAATCGAACATCAAATTGATTTTGTAATTAATACGGCCGGAGTTTTACACAAAGAACCTTTGGAA
ACCATGAATTATGAATACATTCTAAATTCAATAAATGTGAACTATACCGGAATGGTTAATGTTGCGCTTGCGTCACTTCC
GTATTTAAAAGCATCAAAAGGACATATTTTATTTTTCACTTCTAGTTCTTATACCAGAGGCAGGGCATTTTACAGTATTT
ATTCTTCGAGTAAAGCCGCTGCAGTTAATTTTGTACAGGCTGTATCGCAAGAATGGGAAACATTAGGCATTAAGGTAAAT
TGTATGAATCCGGAGCGGACGCAGACTCCAATGCGAATACAAAATTTTGGTAACGAGCCTGAAGATACATTATTAAAACC
CGAAACAGTTGCTTTAAGATGTATTCAGACTCTCTTGTCAAATAATACAGGGCAGGTGGTAGATGTGCGACTTTAA

Upstream 100 bases:

>100_bases
AAAATTTAATACAAAAGAATAATTTAACGGCATCTCAAAACATTATGAATTATCTGGAAACTCAGATTTTTGGGTAATTT
TGAATCAACAAACACAATAC

Downstream 100 bases:

>100_bases
AGCTAAAAGATAAATGCAAAACTCCTCTCTAAAAACACTAGCTGCGAAAGGCATTTTTTGGTCTGCGGTTGATAAATTTG
CCGTTCAGATTGGGCAATTT

Product: 4-diphosphocytidyl-2C-methyl-D-erythritol synthase

Products: NA

Alternate protein names: 4-diphosphocytidyl-2C-methyl-D-erythritol synthase; MEP cytidylyltransferase; MCT [H]

Number of amino acids: Translated: 451; Mature: 451

Protein sequence:

>451_residues
MNIAVILAGGIGIRLEKSLPKQFFKVAGKMIIEHAVDAFEKNEFIDETAIVINKHYLFMAEDMIIKNEWKKVKRVLIGGE
ERYQSSLAAIKSYDEFKDANLIFHDAARPLISQRIINDVVKALSHYKAVDVAINSADTIIEVENDIITAIPERIKMRRGQ
TPQGFKQEIIAKAYEFALKDENFKATDDCGVVKKYLPQEDIYVVNGEEVNMKLTYPEDTYLLDKLFQLRSVEIQDIKLSK
EILSDKVMVVFGGSYGIGKSIVDLAEQNGVKVYSFSRSENNVDVSNIELVKKALEKVFEIEHQIDFVINTAGVLHKEPLE
TMNYEYILNSINVNYTGMVNVALASLPYLKASKGHILFFTSSSYTRGRAFYSIYSSSKAAAVNFVQAVSQEWETLGIKVN
CMNPERTQTPMRIQNFGNEPEDTLLKPETVALRCIQTLLSNNTGQVVDVRL

Sequences:

>Translated_451_residues
MNIAVILAGGIGIRLEKSLPKQFFKVAGKMIIEHAVDAFEKNEFIDETAIVINKHYLFMAEDMIIKNEWKKVKRVLIGGE
ERYQSSLAAIKSYDEFKDANLIFHDAARPLISQRIINDVVKALSHYKAVDVAINSADTIIEVENDIITAIPERIKMRRGQ
TPQGFKQEIIAKAYEFALKDENFKATDDCGVVKKYLPQEDIYVVNGEEVNMKLTYPEDTYLLDKLFQLRSVEIQDIKLSK
EILSDKVMVVFGGSYGIGKSIVDLAEQNGVKVYSFSRSENNVDVSNIELVKKALEKVFEIEHQIDFVINTAGVLHKEPLE
TMNYEYILNSINVNYTGMVNVALASLPYLKASKGHILFFTSSSYTRGRAFYSIYSSSKAAAVNFVQAVSQEWETLGIKVN
CMNPERTQTPMRIQNFGNEPEDTLLKPETVALRCIQTLLSNNTGQVVDVRL
>Mature_451_residues
MNIAVILAGGIGIRLEKSLPKQFFKVAGKMIIEHAVDAFEKNEFIDETAIVINKHYLFMAEDMIIKNEWKKVKRVLIGGE
ERYQSSLAAIKSYDEFKDANLIFHDAARPLISQRIINDVVKALSHYKAVDVAINSADTIIEVENDIITAIPERIKMRRGQ
TPQGFKQEIIAKAYEFALKDENFKATDDCGVVKKYLPQEDIYVVNGEEVNMKLTYPEDTYLLDKLFQLRSVEIQDIKLSK
EILSDKVMVVFGGSYGIGKSIVDLAEQNGVKVYSFSRSENNVDVSNIELVKKALEKVFEIEHQIDFVINTAGVLHKEPLE
TMNYEYILNSINVNYTGMVNVALASLPYLKASKGHILFFTSSSYTRGRAFYSIYSSSKAAAVNFVQAVSQEWETLGIKVN
CMNPERTQTPMRIQNFGNEPEDTLLKPETVALRCIQTLLSNNTGQVVDVRL

Specific function: Catalyzes the formation of 4-diphosphocytidyl-2-C- methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4- phosphate (MEP) [H]

COG id: COG1211

COG function: function code I; 4-diphosphocytidyl-2-methyl-D-erithritol synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispD family [H]

Homologues:

Organism=Homo sapiens, GI157412259, Length=235, Percent_Identity=24.6808510638298, Blast_Score=68, Evalue=2e-11,
Organism=Escherichia coli, GI1789104, Length=228, Percent_Identity=29.3859649122807, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1787526, Length=216, Percent_Identity=27.7777777777778, Blast_Score=67, Evalue=2e-12,
Organism=Drosophila melanogaster, GI23397609, Length=182, Percent_Identity=29.1208791208791, Blast_Score=72, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001228 [H]

Pfam domain/function: PF01128 IspD [H]

EC number: =2.7.7.60 [H]

Molecular weight: Translated: 50940; Mature: 50940

Theoretical pI: Translated: 5.77; Mature: 5.77

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIAVILAGGIGIRLEKSLPKQFFKVAGKMIIEHAVDAFEKNEFIDETAIVINKHYLFMA
CCEEEEEECCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECEEEEEE
EDMIIKNEWKKVKRVLIGGEERYQSSLAAIKSYDEFKDANLIFHDAARPLISQRIINDVV
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHH
KALSHYKAVDVAINSADTIIEVENDIITAIPERIKMRRGQTPQGFKQEIIAKAYEFALKD
HHHHHCEEEEEEECCCCEEEEECCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCC
ENFKATDDCGVVKKYLPQEDIYVVNGEEVNMKLTYPEDTYLLDKLFQLRSVEIQDIKLSK
CCCCCCCCHHHHHHHCCCCCEEEECCCEEEEEEECCCCHHHHHHHHHHHCCEEHHHHHHH
EILSDKVMVVFGGSYGIGKSIVDLAEQNGVKVYSFSRSENNVDVSNIELVKKALEKVFEI
HHHCCCEEEEEECCCCCCHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHH
EHQIDFVINTAGVLHKEPLETMNYEYILNSINVNYTGMVNVALASLPYLKASKGHILFFT
HHHHEEEEECCCHHCCCCHHHCCHHHEEECCCCCEEEHHHHHHHHCCCEECCCCEEEEEE
SSSYTRGRAFYSIYSSSKAAAVNFVQAVSQEWETLGIKVNCMNPERTQTPMRIQNFGNEP
CCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCHHHHHCCCCC
EDTLLKPETVALRCIQTLLSNNTGQVVDVRL
HHHCCCCHHHHHHHHHHHHCCCCCCEEEEEC
>Mature Secondary Structure
MNIAVILAGGIGIRLEKSLPKQFFKVAGKMIIEHAVDAFEKNEFIDETAIVINKHYLFMA
CCEEEEEECCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECEEEEEE
EDMIIKNEWKKVKRVLIGGEERYQSSLAAIKSYDEFKDANLIFHDAARPLISQRIINDVV
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHH
KALSHYKAVDVAINSADTIIEVENDIITAIPERIKMRRGQTPQGFKQEIIAKAYEFALKD
HHHHHCEEEEEEECCCCEEEEECCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCC
ENFKATDDCGVVKKYLPQEDIYVVNGEEVNMKLTYPEDTYLLDKLFQLRSVEIQDIKLSK
CCCCCCCCHHHHHHHCCCCCEEEECCCEEEEEEECCCCHHHHHHHHHHHCCEEHHHHHHH
EILSDKVMVVFGGSYGIGKSIVDLAEQNGVKVYSFSRSENNVDVSNIELVKKALEKVFEI
HHHCCCEEEEEECCCCCCHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHH
EHQIDFVINTAGVLHKEPLETMNYEYILNSINVNYTGMVNVALASLPYLKASKGHILFFT
HHHHEEEEECCCHHCCCCHHHCCHHHEEECCCCCEEEHHHHHHHHCCCEECCCCEEEEEE
SSSYTRGRAFYSIYSSSKAAAVNFVQAVSQEWETLGIKVNCMNPERTQTPMRIQNFGNEP
CCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCHHHHHCCCCC
EDTLLKPETVALRCIQTLLSNNTGQVVDVRL
HHHCCCCHHHHHHHHHHHHCCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA