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The map label for this gene is ldh [H]

Identifier: 146299957

GI number: 146299957

Start: 2655083

End: 2656264

Strand: Direct

Name: ldh [H]

Synonym: Fjoh_2201

Alternate gene names: 146299957

Gene position: 2655083-2656264 (Clockwise)

Preceding gene: 146299952

Following gene: 146299958

Centisome position: 43.55

GC content: 35.7

Gene sequence:

>1182_bases
ATGTGTATTTTTTGTTTATGTTTGGATTGTCTTTTTGACGAATTCATAATTTTTAAACAAATTAAAACTAACACTATGAA
TGCAGCTTTCGCAACTGGAAAGGAACTTCAAAAAATGGACCCTGTTTTTGGTCAGTTATCTTTTGATGATCACGAACAAA
TTGTATTTTGTAATGACAAAGATACAGGTTTAAAAGCAATTATTGGTATTCATAATTCTGTTATGGGACCAGCCTTAGGA
GGCACAAGAATGTGGAATTATAATACCGAGTGGGAAGCATTAAACGATGTTTTGCGTCTTTCCAGAGGTATGACTTATAA
ATCAGCTATTACCGGTCTGAATATTGGCGGTGGAAAAGCGGTTATTATTGGAGATGCAAAAACGCAAAAAACGCCTGAAT
TAATGCGTAAGTTTGGTGAATTTGTACACTCTTTGAGCGGAAGATATATTACAGCTGAAGATGTTGGAATGGAAACTAAA
GACATGGACACTGTAAGAGATGTAACGCCTTATGTTACCGGAATCTCTGAAGAAAGAGGCGGTTCTGGAAATCCTTCTCC
TGTAACAGCTTACGGAGTTTATTTAGGAATGAAAGCTGCTGCTAAAAGTCAGTTTGGTTCTGATGTTCTGGAAGGTAAAA
AAGTTTTGGTTCAGGGAATTGGCCATGTAGGTGAAACTTTGGTTGATTATTTAACTAAAGAAGGAGCGATAGTTACAATT
ACTGATATCAACGAAGAAAAATTATACCAGGCAGCTTCAAAATACAATGCCACTATTTATACAGGTGAAGATTTGTATAC
TGCCGATGTAGATATCTATGCGCCATGTGCAATGGGAGCAACAATTAACGACAATACAGTAGATAAAATTAAAGCTAAAG
TTATTGCCGGAGCGGCAAACAACCAGTTAGCTGATGAAAATGTTCACGGTGCAAGATTACAAGAAAGAGGAATTTTATAC
GCTCCGGATTTCTTAATTAATGCTGGTGGAATCATCAATGTTTATGCTGAATTAGAGCATTATGGCAAAGCTGAGATCAT
GGCTAAAACAGAAAATATCTATAATACAACTTTAGAAATTATCGATTACGCTGCTAAAAACGGAATCACAACTCATAAAG
CAGCACTTACAATTGCTCAAAATCGTATTGATGCAAGAAAAATCGAAAACGCTAAGAAATAA

Upstream 100 bases:

>100_bases
GGGAATTATGAATTATTATTAAATAAAATTATAATAAAATGCAATTTAATCAAAACAATTATATTGTAAAATTGTTATTT
TATGGATAAAAAATTAGCAC

Downstream 100 bases:

>100_bases
TTTTTAGAGTTTTTTAGTATTAGTTTACAGTCTCAGTCAGAGTTTTCAGATTTTGAAAATTGTGACTGGGACTTTTTTTA
TGCAATTCTTTACTGCTGTA

Product: Glu/Leu/Phe/Val dehydrogenase, C terminal

Products: NA

Alternate protein names: LeuDH [H]

Number of amino acids: Translated: 393; Mature: 393

Protein sequence:

>393_residues
MCIFCLCLDCLFDEFIIFKQIKTNTMNAAFATGKELQKMDPVFGQLSFDDHEQIVFCNDKDTGLKAIIGIHNSVMGPALG
GTRMWNYNTEWEALNDVLRLSRGMTYKSAITGLNIGGGKAVIIGDAKTQKTPELMRKFGEFVHSLSGRYITAEDVGMETK
DMDTVRDVTPYVTGISEERGGSGNPSPVTAYGVYLGMKAAAKSQFGSDVLEGKKVLVQGIGHVGETLVDYLTKEGAIVTI
TDINEEKLYQAASKYNATIYTGEDLYTADVDIYAPCAMGATINDNTVDKIKAKVIAGAANNQLADENVHGARLQERGILY
APDFLINAGGIINVYAELEHYGKAEIMAKTENIYNTTLEIIDYAAKNGITTHKAALTIAQNRIDARKIENAKK

Sequences:

>Translated_393_residues
MCIFCLCLDCLFDEFIIFKQIKTNTMNAAFATGKELQKMDPVFGQLSFDDHEQIVFCNDKDTGLKAIIGIHNSVMGPALG
GTRMWNYNTEWEALNDVLRLSRGMTYKSAITGLNIGGGKAVIIGDAKTQKTPELMRKFGEFVHSLSGRYITAEDVGMETK
DMDTVRDVTPYVTGISEERGGSGNPSPVTAYGVYLGMKAAAKSQFGSDVLEGKKVLVQGIGHVGETLVDYLTKEGAIVTI
TDINEEKLYQAASKYNATIYTGEDLYTADVDIYAPCAMGATINDNTVDKIKAKVIAGAANNQLADENVHGARLQERGILY
APDFLINAGGIINVYAELEHYGKAEIMAKTENIYNTTLEIIDYAAKNGITTHKAALTIAQNRIDARKIENAKK
>Mature_393_residues
MCIFCLCLDCLFDEFIIFKQIKTNTMNAAFATGKELQKMDPVFGQLSFDDHEQIVFCNDKDTGLKAIIGIHNSVMGPALG
GTRMWNYNTEWEALNDVLRLSRGMTYKSAITGLNIGGGKAVIIGDAKTQKTPELMRKFGEFVHSLSGRYITAEDVGMETK
DMDTVRDVTPYVTGISEERGGSGNPSPVTAYGVYLGMKAAAKSQFGSDVLEGKKVLVQGIGHVGETLVDYLTKEGAIVTI
TDINEEKLYQAASKYNATIYTGEDLYTADVDIYAPCAMGATINDNTVDKIKAKVIAGAANNQLADENVHGARLQERGILY
APDFLINAGGIINVYAELEHYGKAEIMAKTENIYNTTLEIIDYAAKNGITTHKAALTIAQNRIDARKIENAKK

Specific function: Functions catabolically in the bacterial metabolism of branched-chain L-amino acids. Catalyzes the reversible deamination of these branched-chain L-amino acids to their oxo analogs [H]

COG id: COG0334

COG function: function code E; Glutamate dehydrogenase/leucine dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Glu/Leu/Phe/Val dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI31377775, Length=339, Percent_Identity=25.6637168141593, Blast_Score=67, Evalue=4e-11,
Organism=Drosophila melanogaster, GI45549226, Length=386, Percent_Identity=26.6839378238342, Blast_Score=79, Evalue=7e-15,
Organism=Drosophila melanogaster, GI45553475, Length=386, Percent_Identity=26.6839378238342, Blast_Score=78, Evalue=8e-15,
Organism=Drosophila melanogaster, GI24649460, Length=303, Percent_Identity=28.0528052805281, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24649283, Length=352, Percent_Identity=26.4204545454545, Blast_Score=70, Evalue=3e-12,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 9362 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006095
- InterPro:   IPR006096
- InterPro:   IPR006097
- InterPro:   IPR016211
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00208 ELFV_dehydrog; PF02812 ELFV_dehydrog_N [H]

EC number: =1.4.1.9 [H]

Molecular weight: Translated: 42864; Mature: 42864

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCIFCLCLDCLFDEFIIFKQIKTNTMNAAFATGKELQKMDPVFGQLSFDDHEQIVFCNDK
CCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCHHHHHHCCHHCCCCCCCCCEEEEECCC
DTGLKAIIGIHNSVMGPALGGTRMWNYNTEWEALNDVLRLSRGMTYKSAITGLNIGGGKA
CCCHHEEEEHHHHHCCCCCCCCEEECCCCCHHHHHHHHHHHCCCCHHHHHCCCCCCCCCE
VIIGDAKTQKTPELMRKFGEFVHSLSGRYITAEDVGMETKDMDTVRDVTPYVTGISEERG
EEEECCCCCCCHHHHHHHHHHHHHHCCCEEEHHHCCCCCCCHHHHHHHHHHHCCCCCCCC
GSGNPSPVTAYGVYLGMKAAAKSQFGSDVLEGKKVLVQGIGHVGETLVDYLTKEGAIVTI
CCCCCCCEEEEHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEE
TDINEEKLYQAASKYNATIYTGEDLYTADVDIYAPCAMGATINDNTVDKIKAKVIAGAAN
EECCHHHHHHHHHHCCEEEEECCCEEEECCEEEECCCCCCCCCCCHHHHHHHHEEECCCC
NQLADENVHGARLQERGILYAPDFLINAGGIINVYAELEHYGKAEIMAKTENIYNTTLEI
CCCCCCCCCCCHHHHCCCEECCHHEEECCCEEEEEEHHHHCCCEEEEEECHHHHHHHHHH
IDYAAKNGITTHKAALTIAQNRIDARKIENAKK
HHHHHHCCCCCHHHHEEHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MCIFCLCLDCLFDEFIIFKQIKTNTMNAAFATGKELQKMDPVFGQLSFDDHEQIVFCNDK
CCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCHHHHHHCCHHCCCCCCCCCEEEEECCC
DTGLKAIIGIHNSVMGPALGGTRMWNYNTEWEALNDVLRLSRGMTYKSAITGLNIGGGKA
CCCHHEEEEHHHHHCCCCCCCCEEECCCCCHHHHHHHHHHHCCCCHHHHHCCCCCCCCCE
VIIGDAKTQKTPELMRKFGEFVHSLSGRYITAEDVGMETKDMDTVRDVTPYVTGISEERG
EEEECCCCCCCHHHHHHHHHHHHHHCCCEEEHHHCCCCCCCHHHHHHHHHHHCCCCCCCC
GSGNPSPVTAYGVYLGMKAAAKSQFGSDVLEGKKVLVQGIGHVGETLVDYLTKEGAIVTI
CCCCCCCEEEEHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEE
TDINEEKLYQAASKYNATIYTGEDLYTADVDIYAPCAMGATINDNTVDKIKAKVIAGAAN
EECCHHHHHHHHHHCCEEEEECCCEEEECCEEEECCCCCCCCCCCHHHHHHHHEEECCCC
NQLADENVHGARLQERGILYAPDFLINAGGIINVYAELEHYGKAEIMAKTENIYNTTLEI
CCCCCCCCCCCHHHHCCCEECCHHEEECCCEEEEEEHHHHCCCEEEEEECHHHHHHHHHH
IDYAAKNGITTHKAALTIAQNRIDARKIENAKK
HHHHHHCCCCCHHHHEEHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8020469 [H]