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The map label for this gene is ybiK [C]

Identifier: 146299796

GI number: 146299796

Start: 2393325

End: 2394350

Strand: Direct

Name: ybiK [C]

Synonym: Fjoh_2037

Alternate gene names: 146299796

Gene position: 2393325-2394350 (Clockwise)

Preceding gene: 146299795

Following gene: 146299797

Centisome position: 39.25

GC content: 41.13

Gene sequence:

>1026_bases
ATGTCAAATAGAAGAGATTTTATCAAAAAAGCAGCTTTAGGCGCAGCAGCCGTAAGTTCAGTAGGATTTAGCGGATTTGC
AAAAGAGAAAGAAGATGAAAAAGAAGGCGCATTAGAATTTCCTTCAAAAAAAGTAAAAAAACCGGTTGTTATTTCGACAT
GGAACCACGGTTTACCCGCGAATAAAGAGACCTGGAAAGAATTAAAAGCCGGAAAATCTGCCTTAGACGCGATTGAAGCC
GGTATGAAAATTCCTGAAGCAGATCCGAATGTCCGCAGTGTTGGTTATGGAGGATATCCGGATCGCGAAGGAAAAGTAAC
TCTTGATGCCTGTATTATGGACCACAACAGCAATTGCGGTTCGGTTTGTTTTTTACAAGGTATTATGCATCCTATTTCTG
TAGCCAAAAGGGTATTACAAAATACGCCACACGTAATGCTTGCCGGACAAGGCGCATTGCAGTTTGCTTTATCAGAAGGT
TTTAAAGAAGAAAATTTACTAACTCCGGAATCTGAAAAAGACTGGAAAAAATGGCTGGAAGATTCTAAATACAAACCAGT
TATTAATATAGAAAATCACGACACCATAAGTATGCTAATGTTGGATCAAGACGGAAATCTTTCGGGAGGGTGTACCACCA
GTGGTGCTGCCTGGAAGATGCACGGCCGCGTCGGTGACTCCCCAATAATCGGCGCGGGTCTTTTTCTGGATAACGAAGTA
GGTGCTGCGGCCGCTACAGGTTTAGGCGAAGCAGTAATTAGAACAGCAGGAAGTGCCATGGTTGTAGAATTAATGCGTCA
GGGAAAATCGCCTTATGATGCCTGCAAAGAAATCACAGAGCGTATTTACAACAAACACAAAAACCACAAAGACATGGAGT
ATCTGCAAGTTGGATTTATTGCCTTAAATAAAAACGGCGAATATGCAGGTTACAGTTTGAGATCTGGTTTTAATTATGCC
GTTTCTGATGATGAAAAAGGACACAGAATGGAAGACGCTAAATTTAAAATGTCGTGGGATAAATAA

Upstream 100 bases:

>100_bases
AACACATAGCTATGTTTGCTAATGCAAGTGAAACGCCTTTATTTAGTTTCCAAAACTATGTTTCTATGTGTTTAAATGAA
TTAAGATAAAAGTAAGCAGC

Downstream 100 bases:

>100_bases
ACCTTTTTGTCATTTCAACGAAGGAGAAATCACACGTAACTCGACAAAGATTGGCGATTAAAATTTGAAGCACATAGAAA
TAGATTTTTCTCTCACAATC

Product: N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase

Products: NA

Alternate protein names: Aspartylglucosaminidase; AGA; Glycosylasparaginase; N4-(N-acetyl-beta-glucosaminyl)-L-asparagine amidase [H]

Number of amino acids: Translated: 341; Mature: 340

Protein sequence:

>341_residues
MSNRRDFIKKAALGAAAVSSVGFSGFAKEKEDEKEGALEFPSKKVKKPVVISTWNHGLPANKETWKELKAGKSALDAIEA
GMKIPEADPNVRSVGYGGYPDREGKVTLDACIMDHNSNCGSVCFLQGIMHPISVAKRVLQNTPHVMLAGQGALQFALSEG
FKEENLLTPESEKDWKKWLEDSKYKPVINIENHDTISMLMLDQDGNLSGGCTTSGAAWKMHGRVGDSPIIGAGLFLDNEV
GAAAATGLGEAVIRTAGSAMVVELMRQGKSPYDACKEITERIYNKHKNHKDMEYLQVGFIALNKNGEYAGYSLRSGFNYA
VSDDEKGHRMEDAKFKMSWDK

Sequences:

>Translated_341_residues
MSNRRDFIKKAALGAAAVSSVGFSGFAKEKEDEKEGALEFPSKKVKKPVVISTWNHGLPANKETWKELKAGKSALDAIEA
GMKIPEADPNVRSVGYGGYPDREGKVTLDACIMDHNSNCGSVCFLQGIMHPISVAKRVLQNTPHVMLAGQGALQFALSEG
FKEENLLTPESEKDWKKWLEDSKYKPVINIENHDTISMLMLDQDGNLSGGCTTSGAAWKMHGRVGDSPIIGAGLFLDNEV
GAAAATGLGEAVIRTAGSAMVVELMRQGKSPYDACKEITERIYNKHKNHKDMEYLQVGFIALNKNGEYAGYSLRSGFNYA
VSDDEKGHRMEDAKFKMSWDK
>Mature_340_residues
SNRRDFIKKAALGAAAVSSVGFSGFAKEKEDEKEGALEFPSKKVKKPVVISTWNHGLPANKETWKELKAGKSALDAIEAG
MKIPEADPNVRSVGYGGYPDREGKVTLDACIMDHNSNCGSVCFLQGIMHPISVAKRVLQNTPHVMLAGQGALQFALSEGF
KEENLLTPESEKDWKKWLEDSKYKPVINIENHDTISMLMLDQDGNLSGGCTTSGAAWKMHGRVGDSPIIGAGLFLDNEVG
AAAATGLGEAVIRTAGSAMVVELMRQGKSPYDACKEITERIYNKHKNHKDMEYLQVGFIALNKNGEYAGYSLRSGFNYAV
SDDEKGHRMEDAKFKMSWDK

Specific function: Cleaves the GlcNAc-Asn bond which joins oligosaccharides to the peptide of asparagine-linked glycoproteins. Requires that the glycosylated asparagine moiety is not substituted on its N- (R1) and C- (R2) terminus [H]

COG id: COG1446

COG function: function code E; Asparaginase

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Ntn-hydrolase family [H]

Homologues:

Organism=Homo sapiens, GI285002251, Length=321, Percent_Identity=35.5140186915888, Blast_Score=155, Evalue=7e-38,
Organism=Homo sapiens, GI285002253, Length=321, Percent_Identity=33.6448598130841, Blast_Score=133, Evalue=3e-31,
Organism=Homo sapiens, GI145275202, Length=265, Percent_Identity=32.0754716981132, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI145275200, Length=265, Percent_Identity=32.0754716981132, Blast_Score=124, Evalue=1e-28,
Organism=Escherichia coli, GI1787050, Length=241, Percent_Identity=32.7800829875519, Blast_Score=115, Evalue=4e-27,
Organism=Caenorhabditis elegans, GI17569239, Length=284, Percent_Identity=33.0985915492958, Blast_Score=119, Evalue=3e-27,
Organism=Drosophila melanogaster, GI221330102, Length=317, Percent_Identity=39.1167192429022, Blast_Score=179, Evalue=3e-45,
Organism=Drosophila melanogaster, GI24652135, Length=316, Percent_Identity=38.9240506329114, Blast_Score=175, Evalue=4e-44,
Organism=Drosophila melanogaster, GI24655553, Length=337, Percent_Identity=32.3442136498516, Blast_Score=153, Evalue=1e-37,
Organism=Drosophila melanogaster, GI24657358, Length=319, Percent_Identity=31.0344827586207, Blast_Score=130, Evalue=1e-30,
Organism=Drosophila melanogaster, GI18921183, Length=236, Percent_Identity=31.7796610169492, Blast_Score=108, Evalue=4e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000246
- InterPro:   IPR006311 [H]

Pfam domain/function: PF01112 Asparaginase_2 [H]

EC number: =3.5.1.26 [H]

Molecular weight: Translated: 37284; Mature: 37153

Theoretical pI: Translated: 7.00; Mature: 7.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNRRDFIKKAALGAAAVSSVGFSGFAKEKEDEKEGALEFPSKKVKKPVVISTWNHGLPA
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHCCCCCCCHHHCCCCEEEEECCCCCCC
NKETWKELKAGKSALDAIEAGMKIPEADPNVRSVGYGGYPDREGKVTLDACIMDHNSNCG
CHHHHHHHHHCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEECCCCCC
SVCFLQGIMHPISVAKRVLQNTPHVMLAGQGALQFALSEGFKEENLLTPESEKDWKKWLE
HHHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHCCCCCCCCCCCCCHHHHHHHHH
DSKYKPVINIENHDTISMLMLDQDGNLSGGCTTSGAAWKMHGRVGDSPIIGAGLFLDNEV
CCCCCCEEEECCCCEEEEEEEECCCCCCCCCCCCCCEEEECCCCCCCCEEEEEEEECCCC
GAAAATGLGEAVIRTAGSAMVVELMRQGKSPYDACKEITERIYNKHKNHKDMEYLQVGFI
CHHHHHHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCHHHEEEEEE
ALNKNGEYAGYSLRSGFNYAVSDDEKGHRMEDAKFKMSWDK
EECCCCCCCCEECCCCCCEEECCCCCCCCCCCCEEEECCCC
>Mature Secondary Structure 
SNRRDFIKKAALGAAAVSSVGFSGFAKEKEDEKEGALEFPSKKVKKPVVISTWNHGLPA
CCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHCCCCCCCHHHCCCCEEEEECCCCCCC
NKETWKELKAGKSALDAIEAGMKIPEADPNVRSVGYGGYPDREGKVTLDACIMDHNSNCG
CHHHHHHHHHCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEECCCCCC
SVCFLQGIMHPISVAKRVLQNTPHVMLAGQGALQFALSEGFKEENLLTPESEKDWKKWLE
HHHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHCCCCCCCCCCCCCHHHHHHHHH
DSKYKPVINIENHDTISMLMLDQDGNLSGGCTTSGAAWKMHGRVGDSPIIGAGLFLDNEV
CCCCCCEEEECCCCEEEEEEEECCCCCCCCCCCCCCEEEECCCCCCCCEEEEEEEECCCC
GAAAATGLGEAVIRTAGSAMVVELMRQGKSPYDACKEITERIYNKHKNHKDMEYLQVGFI
CHHHHHHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCHHHEEEEEE
ALNKNGEYAGYSLRSGFNYAVSDDEKGHRMEDAKFKMSWDK
EECCCCCCCCEECCCCCCEEECCCCCCCCCCCCEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7840643; 8250923; 9541410 [H]