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The map label for this gene is tuf

Identifier: 146299696

GI number: 146299696

Start: 2238888

End: 2240075

Strand: Direct

Name: tuf

Synonym: Fjoh_1936

Alternate gene names: 146299696

Gene position: 2238888-2240075 (Clockwise)

Preceding gene: 146299695

Following gene: 146299697

Centisome position: 36.72

GC content: 40.15

Gene sequence:

>1188_bases
ATGGCAAAGGAGAATTTTAATCGTTCCAAACCGCACTTAAACATAGGTACAATTGGACACGTAGATCACGGAAAAACTAC
ATTAACTGCTGCAATTACAAAAGTATTGTCAGATGCTGGTTACTGTCAAGCAAAATCGTTTGATCAAATCGATAACGCTC
CAGAGGAGAAAGAAAGAGGTATTACTATTAATACATCACACGTAGAGTACGAAACAGCTAACCGTCACTACGCTCACGTT
GACTGTCCAGGTCACGCGGATTACGTAAAGAACATGGTTACTGGTGCTGCTCAAATGGACGGTGCTATCTTAGTAGTTGC
TGCTACAGATGGTCCAATGCCACAAACTCGTGAGCACATCCTTTTAGGACGTCAGGTTGGTATTCCAAGAATCGTTGTTT
TCATGAACAAAGTGGATATGGTTGATGATGCTGAGTTATTAGAGCTTGTTGAAATGGAAATTAGAGATTTATTATCTTTC
TACGAATATGATGGAGATAATGGTCCTGTAGTTCAAGGTTCTGCTTTAGGAGGATTAAACAATGATCCAAACTGGGTACC
TAAAATTATCGAATTAATGGAAGCTGTTGATAACTGGATCGAAGAGCCAGTACGTGACGTTGCTAAACCATTCTTGATGC
CAGTTGAGGACGTATTTACAATTACAGGTCGTGGAACTGTTGCTACAGGTCGTATCGAAACTGGGGTTGCTAACACTGGA
GATCCTGTTGAGATCATCGGTATGGGAGCTGAAAAATTAACTTCTACTATTACAGGAGTTGAGATGTTCCGTAAAATCCT
TGATAGAGGTGAAGCTGGAGATAACGTAGGTTTATTGTTAAGAGGTATTGATAAAGCTGATATCAAAAGAGGTATGGTTA
TTATTAAGCCAGGTTCAGTAAAACCACACGCTAAATTCAAAGCTGAGGTTTATATCTTGAAAAAAGAAGAAGGTGGACGT
CACACTCCATTCCACAATAACTACCGTCCACAGTTCTACGTACGTACAACTGACGTAACAGGAGTTATTTCTTTACCAGC
AGGTGTAGAGATGGTAATGCCAGGTGATAACTTAACTATTGAGGTTGCTTTATTAAGTCCAATCGCTATGAACGTAGGTT
TACGTTTCGCTATCCGTGAAGGTGGTAGAACAGTTGGTGCTGGACAAGTTACTGAAATCGTAGAGTAA

Upstream 100 bases:

>100_bases
TCCTTGGTAAGGAAGAGGTCTCGGGTTCAAATCCCGACATTGGCTCAAGTAAGTAGGAAATTGAAAATTAATATATAACT
AAGATTAAAAATTAAGTAAA

Downstream 100 bases:

>100_bases
TCTATAATAATAAAAACAAAAGCCAGTTGATGTCTTAACTGAAGTCACTGGCTTTTTTAATTACGGGCGTAGTTCAAGGG
TAGAATAGCGGTCTCCAAAA

Product: elongation factor Tu

Products: GDP; phosphate

Alternate protein names: EF-Tu

Number of amino acids: Translated: 395; Mature: 394

Protein sequence:

>395_residues
MAKENFNRSKPHLNIGTIGHVDHGKTTLTAAITKVLSDAGYCQAKSFDQIDNAPEEKERGITINTSHVEYETANRHYAHV
DCPGHADYVKNMVTGAAQMDGAILVVAATDGPMPQTREHILLGRQVGIPRIVVFMNKVDMVDDAELLELVEMEIRDLLSF
YEYDGDNGPVVQGSALGGLNNDPNWVPKIIELMEAVDNWIEEPVRDVAKPFLMPVEDVFTITGRGTVATGRIETGVANTG
DPVEIIGMGAEKLTSTITGVEMFRKILDRGEAGDNVGLLLRGIDKADIKRGMVIIKPGSVKPHAKFKAEVYILKKEEGGR
HTPFHNNYRPQFYVRTTDVTGVISLPAGVEMVMPGDNLTIEVALLSPIAMNVGLRFAIREGGRTVGAGQVTEIVE

Sequences:

>Translated_395_residues
MAKENFNRSKPHLNIGTIGHVDHGKTTLTAAITKVLSDAGYCQAKSFDQIDNAPEEKERGITINTSHVEYETANRHYAHV
DCPGHADYVKNMVTGAAQMDGAILVVAATDGPMPQTREHILLGRQVGIPRIVVFMNKVDMVDDAELLELVEMEIRDLLSF
YEYDGDNGPVVQGSALGGLNNDPNWVPKIIELMEAVDNWIEEPVRDVAKPFLMPVEDVFTITGRGTVATGRIETGVANTG
DPVEIIGMGAEKLTSTITGVEMFRKILDRGEAGDNVGLLLRGIDKADIKRGMVIIKPGSVKPHAKFKAEVYILKKEEGGR
HTPFHNNYRPQFYVRTTDVTGVISLPAGVEMVMPGDNLTIEVALLSPIAMNVGLRFAIREGGRTVGAGQVTEIVE
>Mature_394_residues
AKENFNRSKPHLNIGTIGHVDHGKTTLTAAITKVLSDAGYCQAKSFDQIDNAPEEKERGITINTSHVEYETANRHYAHVD
CPGHADYVKNMVTGAAQMDGAILVVAATDGPMPQTREHILLGRQVGIPRIVVFMNKVDMVDDAELLELVEMEIRDLLSFY
EYDGDNGPVVQGSALGGLNNDPNWVPKIIELMEAVDNWIEEPVRDVAKPFLMPVEDVFTITGRGTVATGRIETGVANTGD
PVEIIGMGAEKLTSTITGVEMFRKILDRGEAGDNVGLLLRGIDKADIKRGMVIIKPGSVKPHAKFKAEVYILKKEEGGRH
TPFHNNYRPQFYVRTTDVTGVISLPAGVEMVMPGDNLTIEVALLSPIAMNVGLRFAIREGGRTVGAGQVTEIVE

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis

COG id: COG0050

COG function: function code J; GTPases - translation elongation factors

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily

Homologues:

Organism=Homo sapiens, GI34147630, Length=392, Percent_Identity=54.8469387755102, Blast_Score=461, Evalue=1e-130,
Organism=Homo sapiens, GI223555963, Length=437, Percent_Identity=29.9771167048055, Blast_Score=164, Evalue=1e-40,
Organism=Homo sapiens, GI5729864, Length=437, Percent_Identity=29.9771167048055, Blast_Score=164, Evalue=2e-40,
Organism=Homo sapiens, GI4503475, Length=449, Percent_Identity=29.6213808463252, Blast_Score=160, Evalue=1e-39,
Organism=Homo sapiens, GI4503471, Length=443, Percent_Identity=28.4424379232506, Blast_Score=155, Evalue=7e-38,
Organism=Homo sapiens, GI54607086, Length=314, Percent_Identity=28.343949044586, Blast_Score=132, Evalue=6e-31,
Organism=Homo sapiens, GI194097354, Length=336, Percent_Identity=27.6785714285714, Blast_Score=105, Evalue=8e-23,
Organism=Homo sapiens, GI194018520, Length=336, Percent_Identity=27.6785714285714, Blast_Score=105, Evalue=8e-23,
Organism=Homo sapiens, GI194018522, Length=337, Percent_Identity=27.5964391691395, Blast_Score=105, Evalue=8e-23,
Organism=Homo sapiens, GI46094014, Length=367, Percent_Identity=26.158038147139, Blast_Score=102, Evalue=7e-22,
Organism=Homo sapiens, GI4503507, Length=267, Percent_Identity=28.4644194756554, Blast_Score=86, Evalue=6e-17,
Organism=Homo sapiens, GI94966754, Length=135, Percent_Identity=34.8148148148148, Blast_Score=70, Evalue=4e-12,
Organism=Escherichia coli, GI1790412, Length=394, Percent_Identity=69.7969543147208, Blast_Score=549, Evalue=1e-158,
Organism=Escherichia coli, GI1789737, Length=394, Percent_Identity=69.7969543147208, Blast_Score=549, Evalue=1e-157,
Organism=Escherichia coli, GI2367247, Length=380, Percent_Identity=28.9473684210526, Blast_Score=117, Evalue=2e-27,
Organism=Escherichia coli, GI1789108, Length=330, Percent_Identity=28.4848484848485, Blast_Score=99, Evalue=4e-22,
Organism=Escherichia coli, GI48994988, Length=282, Percent_Identity=31.2056737588652, Blast_Score=92, Evalue=8e-20,
Organism=Escherichia coli, GI1788922, Length=279, Percent_Identity=28.3154121863799, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI1790835, Length=149, Percent_Identity=30.8724832214765, Blast_Score=64, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17556456, Length=399, Percent_Identity=51.1278195488722, Blast_Score=394, Evalue=1e-110,
Organism=Caenorhabditis elegans, GI25141371, Length=386, Percent_Identity=42.2279792746114, Blast_Score=317, Evalue=5e-87,
Organism=Caenorhabditis elegans, GI17552884, Length=444, Percent_Identity=28.8288288288288, Blast_Score=156, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI17569207, Length=444, Percent_Identity=28.8288288288288, Blast_Score=156, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI32566303, Length=429, Percent_Identity=27.5058275058275, Blast_Score=129, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI115532067, Length=439, Percent_Identity=27.3348519362187, Blast_Score=120, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI115532065, Length=436, Percent_Identity=27.2935779816514, Blast_Score=119, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI32566629, Length=450, Percent_Identity=26.2222222222222, Blast_Score=119, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI17506081, Length=272, Percent_Identity=29.0441176470588, Blast_Score=114, Evalue=8e-26,
Organism=Caenorhabditis elegans, GI32566301, Length=166, Percent_Identity=34.3373493975904, Blast_Score=91, Evalue=8e-19,
Organism=Caenorhabditis elegans, GI17509919, Length=292, Percent_Identity=28.4246575342466, Blast_Score=86, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17557151, Length=235, Percent_Identity=27.6595744680851, Blast_Score=81, Evalue=8e-16,
Organism=Saccharomyces cerevisiae, GI6324761, Length=396, Percent_Identity=61.8686868686869, Blast_Score=506, Evalue=1e-144,
Organism=Saccharomyces cerevisiae, GI6325337, Length=348, Percent_Identity=34.7701149425287, Blast_Score=160, Evalue=3e-40,
Organism=Saccharomyces cerevisiae, GI6319594, Length=348, Percent_Identity=34.7701149425287, Blast_Score=160, Evalue=3e-40,
Organism=Saccharomyces cerevisiae, GI6322937, Length=469, Percent_Identity=24.0938166311301, Blast_Score=96, Evalue=9e-21,
Organism=Saccharomyces cerevisiae, GI6320863, Length=281, Percent_Identity=29.8932384341637, Blast_Score=88, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6320377, Length=350, Percent_Identity=25.7142857142857, Blast_Score=87, Evalue=4e-18,
Organism=Saccharomyces cerevisiae, GI6323098, Length=148, Percent_Identity=31.0810810810811, Blast_Score=66, Evalue=9e-12,
Organism=Saccharomyces cerevisiae, GI6323320, Length=250, Percent_Identity=28.8, Blast_Score=64, Evalue=3e-11,
Organism=Drosophila melanogaster, GI281363316, Length=394, Percent_Identity=56.3451776649746, Blast_Score=453, Evalue=1e-127,
Organism=Drosophila melanogaster, GI17864358, Length=394, Percent_Identity=56.3451776649746, Blast_Score=453, Evalue=1e-127,
Organism=Drosophila melanogaster, GI19921738, Length=387, Percent_Identity=49.8708010335917, Blast_Score=392, Evalue=1e-109,
Organism=Drosophila melanogaster, GI24652838, Length=443, Percent_Identity=27.765237020316, Blast_Score=147, Evalue=1e-35,
Organism=Drosophila melanogaster, GI17137572, Length=443, Percent_Identity=27.765237020316, Blast_Score=147, Evalue=1e-35,
Organism=Drosophila melanogaster, GI45553807, Length=449, Percent_Identity=27.1714922048998, Blast_Score=145, Evalue=4e-35,
Organism=Drosophila melanogaster, GI45553816, Length=449, Percent_Identity=27.1714922048998, Blast_Score=145, Evalue=4e-35,
Organism=Drosophila melanogaster, GI24651721, Length=449, Percent_Identity=27.1714922048998, Blast_Score=145, Evalue=4e-35,
Organism=Drosophila melanogaster, GI17864154, Length=449, Percent_Identity=27.1714922048998, Blast_Score=145, Evalue=4e-35,
Organism=Drosophila melanogaster, GI19922690, Length=304, Percent_Identity=30.2631578947368, Blast_Score=140, Evalue=1e-33,
Organism=Drosophila melanogaster, GI45550900, Length=340, Percent_Identity=28.8235294117647, Blast_Score=122, Evalue=3e-28,
Organism=Drosophila melanogaster, GI17137380, Length=352, Percent_Identity=26.9886363636364, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24647046, Length=291, Percent_Identity=28.5223367697594, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24647048, Length=291, Percent_Identity=28.5223367697594, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI28572034, Length=172, Percent_Identity=34.8837209302326, Blast_Score=65, Evalue=7e-11,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): EFTU_FLAJ1 (A5FIJ9)

Other databases:

- EMBL:   CP000685
- RefSeq:   YP_001194287.1
- ProteinModelPortal:   A5FIJ9
- SMR:   A5FIJ9
- STRING:   A5FIJ9
- GeneID:   5093552
- GenomeReviews:   CP000685_GR
- KEGG:   fjo:Fjoh_1936
- eggNOG:   COG0050
- HOGENOM:   HBG307581
- OMA:   FEGQVYV
- ProtClustDB:   PRK12735
- BioCyc:   FJOH376686:FJOH_1936-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00118_B
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR004541
- InterPro:   IPR004160
- InterPro:   IPR009000
- PANTHER:   PTHR23115:SF31
- PRINTS:   PR00315
- TIGRFAMs:   TIGR00485
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3; SSF50465 Elong_init_C; SSF50447 Translat_factor

EC number: 3.6.5.3

Molecular weight: Translated: 43052; Mature: 42921

Theoretical pI: Translated: 5.08; Mature: 5.08

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKENFNRSKPHLNIGTIGHVDHGKTTLTAAITKVLSDAGYCQAKSFDQIDNAPEEKERG
CCCCCCCCCCCCEEECEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHCCCCCHHHCC
ITINTSHVEYETANRHYAHVDCPGHADYVKNMVTGAAQMDGAILVVAATDGPMPQTREHI
EEEECCEEEEECCCCEEEEEECCCHHHHHHHHHHCHHHCCCEEEEEEECCCCCCCCHHHE
LLGRQVGIPRIVVFMNKVDMVDDAELLELVEMEIRDLLSFYEYDGDNGPVVQGSALGGLN
EECCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCC
NDPNWVPKIIELMEAVDNWIEEPVRDVAKPFLMPVEDVFTITGRGTVATGRIETGVANTG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHEEEECCCEEEECCEECCCCCCC
DPVEIIGMGAEKLTSTITGVEMFRKILDRGEAGDNVGLLLRGIDKADIKRGMVIIKPGSV
CCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHCCEEEEECCCC
KPHAKFKAEVYILKKEEGGRHTPFHNNYRPQFYVRTTDVTGVISLPAGVEMVMPGDNLTI
CCCCEEEEEEEEEEECCCCCCCCCCCCCCCEEEEEEECCEEEEECCCCEEEEECCCCCEE
EVALLSPIAMNVGLRFAIREGGRTVGAGQVTEIVE
EEEEECCHHHCCCEEEEEECCCCEECCCHHHHHCC
>Mature Secondary Structure 
AKENFNRSKPHLNIGTIGHVDHGKTTLTAAITKVLSDAGYCQAKSFDQIDNAPEEKERG
CCCCCCCCCCCEEECEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHCCCCCHHHCC
ITINTSHVEYETANRHYAHVDCPGHADYVKNMVTGAAQMDGAILVVAATDGPMPQTREHI
EEEECCEEEEECCCCEEEEEECCCHHHHHHHHHHCHHHCCCEEEEEEECCCCCCCCHHHE
LLGRQVGIPRIVVFMNKVDMVDDAELLELVEMEIRDLLSFYEYDGDNGPVVQGSALGGLN
EECCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCC
NDPNWVPKIIELMEAVDNWIEEPVRDVAKPFLMPVEDVFTITGRGTVATGRIETGVANTG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHEEEECCCEEEECCEECCCCCCC
DPVEIIGMGAEKLTSTITGVEMFRKILDRGEAGDNVGLLLRGIDKADIKRGMVIIKPGSV
CCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHCCEEEEECCCC
KPHAKFKAEVYILKKEEGGRHTPFHNNYRPQFYVRTTDVTGVISLPAGVEMVMPGDNLTI
CCCCEEEEEEEEEEECCCCCCCCCCCCCCCEEEEEEECCEEEEECCCCEEEEECCCCCEE
EVALLSPIAMNVGLRFAIREGGRTVGAGQVTEIVE
EEEEECCHHHCCCEEEEEECCCCEECCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA