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The map label for this gene is ybfF [C]

Identifier: 146299459

GI number: 146299459

Start: 1969720

End: 1970484

Strand: Direct

Name: ybfF [C]

Synonym: Fjoh_1699

Alternate gene names: 146299459

Gene position: 1969720-1970484 (Clockwise)

Preceding gene: 146299457

Following gene: 146299460

Centisome position: 32.31

GC content: 36.34

Gene sequence:

>765_bases
ATGTTATACTCAAAAATAGAAGGCGAAGGAAAACCTTTTATCATCATGCACGGATTTCTCGGCATGTCTGATAACTGGAA
AACTTTGGCAGGACAATATGTAGAAGCCGGATTTCAGGTTCATATTTTAGATTTAAGAAACCACGGACGCAGTTTTCATT
CAGATGAATGGAGTTATGAAGCCATGGTTCAGGATGTTTTTGAGTATTGTCAGGCAAACCAATTAACCAGAATTGATATT
CTGGGACATTCGATGGGCGGAAAAGTGGCTATGCTTTTTGCAGCGCTGCATCCTGAAATTGTAGACAAATTGATCGTGGC
AGATATTGGTCCGAGATTCTATAAACAGCATCATCAGGATATTTTGGCAGGTTTAAATGCGGTCGATTTTTCGGTAAAAC
CTAGTAGAAATGATGTCGAAGAAATTGTATCTCAATACGTTTCTGATTTTGGGACGCGCCAGTTTTTATTGAAAAATTTA
TATTGGAAAGAACCCGGGCAATTAGCATTTAGGTTCAATTTAGAAGCATTTAATAATAATCTCGATGCTATTGGAAAACC
TTTGGCAGAAAACCTGAAATTTGATAAATCAACCTTATTTATAAGAGGAGGAAATTCAGGCTATATTCAGGATTCAGATT
TGGATGCTATTAAAACGCAGTTCCCCAATTTTAAATTAGAAACTATTCCAAATGCAGGGCATTGGCTTCATGCCGAAAAT
CCAAAAATGTTTTTTGAATTGACAACAGCATTTTTGAAAGAGTAA

Upstream 100 bases:

>100_bases
CTGTTAATCTTTTTCATAAAAAATATTTTGAGAATATATTCTACAAGTGTTTAATAGATAAGTTTTTTACATTTGTAGAA
GAATTTTAAAGACAAAATAA

Downstream 100 bases:

>100_bases
TAATTTTTAATTTGAGACTAAAAAAAATAAGATTTCGGTCATTGAACAATATAAAATCATTATTTTTAAATAAATTTTAA
ACCTGAATAACTATGAAATT

Product: alpha/beta hydrolase fold protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 254; Mature: 254

Protein sequence:

>254_residues
MLYSKIEGEGKPFIIMHGFLGMSDNWKTLAGQYVEAGFQVHILDLRNHGRSFHSDEWSYEAMVQDVFEYCQANQLTRIDI
LGHSMGGKVAMLFAALHPEIVDKLIVADIGPRFYKQHHQDILAGLNAVDFSVKPSRNDVEEIVSQYVSDFGTRQFLLKNL
YWKEPGQLAFRFNLEAFNNNLDAIGKPLAENLKFDKSTLFIRGGNSGYIQDSDLDAIKTQFPNFKLETIPNAGHWLHAEN
PKMFFELTTAFLKE

Sequences:

>Translated_254_residues
MLYSKIEGEGKPFIIMHGFLGMSDNWKTLAGQYVEAGFQVHILDLRNHGRSFHSDEWSYEAMVQDVFEYCQANQLTRIDI
LGHSMGGKVAMLFAALHPEIVDKLIVADIGPRFYKQHHQDILAGLNAVDFSVKPSRNDVEEIVSQYVSDFGTRQFLLKNL
YWKEPGQLAFRFNLEAFNNNLDAIGKPLAENLKFDKSTLFIRGGNSGYIQDSDLDAIKTQFPNFKLETIPNAGHWLHAEN
PKMFFELTTAFLKE
>Mature_254_residues
MLYSKIEGEGKPFIIMHGFLGMSDNWKTLAGQYVEAGFQVHILDLRNHGRSFHSDEWSYEAMVQDVFEYCQANQLTRIDI
LGHSMGGKVAMLFAALHPEIVDKLIVADIGPRFYKQHHQDILAGLNAVDFSVKPSRNDVEEIVSQYVSDFGTRQFLLKNL
YWKEPGQLAFRFNLEAFNNNLDAIGKPLAENLKFDKSTLFIRGGNSGYIQDSDLDAIKTQFPNFKLETIPNAGHWLHAEN
PKMFFELTTAFLKE

Specific function: Unknown

COG id: COG0596

COG function: function code R; Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dmpD/todF/xylF esterase family [H]

Homologues:

Organism=Homo sapiens, GI23200008, Length=259, Percent_Identity=34.7490347490347, Blast_Score=149, Evalue=3e-36,
Organism=Homo sapiens, GI23200012, Length=256, Percent_Identity=34.765625, Blast_Score=147, Evalue=1e-35,
Organism=Homo sapiens, GI223941839, Length=253, Percent_Identity=28.4584980237154, Blast_Score=98, Evalue=8e-21,
Organism=Escherichia coli, GI1786902, Length=245, Percent_Identity=33.469387755102, Blast_Score=137, Evalue=9e-34,
Organism=Caenorhabditis elegans, GI17508535, Length=255, Percent_Identity=32.156862745098, Blast_Score=152, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI17507063, Length=266, Percent_Identity=25.5639097744361, Blast_Score=90, Evalue=9e-19,
Organism=Saccharomyces cerevisiae, GI6321468, Length=278, Percent_Identity=28.7769784172662, Blast_Score=93, Evalue=5e-20,
Organism=Drosophila melanogaster, GI24640348, Length=253, Percent_Identity=29.2490118577075, Blast_Score=108, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24646055, Length=259, Percent_Identity=23.5521235521236, Blast_Score=80, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR012908 [H]

Pfam domain/function: PF00561 Abhydrolase_1; PF07819 PGAP1 [H]

EC number: 3.1.-.- [C]

Molecular weight: Translated: 28982; Mature: 28982

Theoretical pI: Translated: 5.98; Mature: 5.98

Prosite motif: PS00120 LIPASE_SER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLYSKIEGEGKPFIIMHGFLGMSDNWKTLAGQYVEAGFQVHILDLRNHGRSFHSDEWSYE
CCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCHHH
AMVQDVFEYCQANQLTRIDILGHSMGGKVAMLFAALHPEIVDKLIVADIGPRFYKQHHQD
HHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHCHHHHHHHHHHHCCHHHHHHHHHH
ILAGLNAVDFSVKPSRNDVEEIVSQYVSDFGTRQFLLKNLYWKEPGQLAFRFNLEAFNNN
HHHCCCCEEEEECCCCCHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCEEEEEEHHHHCCC
LDAIGKPLAENLKFDKSTLFIRGGNSGYIQDSDLDAIKTQFPNFKLETIPNAGHWLHAEN
HHHHCCHHHHCCCCCCEEEEEECCCCCCEECCCHHHHHHHCCCEEEEECCCCCCEEECCC
PKMFFELTTAFLKE
CHHHHHHHHHHHCC
>Mature Secondary Structure
MLYSKIEGEGKPFIIMHGFLGMSDNWKTLAGQYVEAGFQVHILDLRNHGRSFHSDEWSYE
CCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCHHH
AMVQDVFEYCQANQLTRIDILGHSMGGKVAMLFAALHPEIVDKLIVADIGPRFYKQHHQD
HHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHCHHHHHHHHHHHCCHHHHHHHHHH
ILAGLNAVDFSVKPSRNDVEEIVSQYVSDFGTRQFLLKNLYWKEPGQLAFRFNLEAFNNN
HHHCCCCEEEEECCCCCHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCEEEEEEHHHHCCC
LDAIGKPLAENLKFDKSTLFIRGGNSGYIQDSDLDAIKTQFPNFKLETIPNAGHWLHAEN
HHHHCCHHHHCCCCCCEEEEEECCCCCCEECCCHHHHHHHCCCEEEEECCCCCCEEECCC
PKMFFELTTAFLKE
CHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]