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The map label for this gene is gcvH [H]

Identifier: 146299414

GI number: 146299414

Start: 1924996

End: 1925376

Strand: Direct

Name: gcvH [H]

Synonym: Fjoh_1654

Alternate gene names: 146299414

Gene position: 1924996-1925376 (Clockwise)

Preceding gene: 146299413

Following gene: 146299415

Centisome position: 31.57

GC content: 34.12

Gene sequence:

>381_bases
ATGAGCATACCAGCAAATTTAAAGTACACAAAAGATCACGAATGGGTTAGCATCGAAGGAGATGTTGCGACTGTAGGAAT
TACTCATTTTGCACAAAAAGAGTTAGGGGATATCGTGTATGTTGAAGTAGAAACTTTAGATCAGACACTTGATAAAGATG
AGGTTTTTGGAACAGTTGAAGCTGTAAAAACAGTTTCAGATTTGTTTTTGCCTTTAACAGGAGAAATTATTGCTTTTAAT
GAAGATTTAGAAAGTGCGCCAGAAACAGTAAATTCTGATCCTTATGGAGCTGGATGGATGATTAAAGTTAAAATTGCTAA
TCCTTCAGAAATAGATTCTTTATTATCTGACGAAGCTTATAAACAATTAATCGGTGCCTAA

Upstream 100 bases:

>100_bases
TAATTTCGGAAATTAAATTTAGGTTTCTAAACCATTATTATTAGAAGATTATTACATTTGTTGCAGAATTATTAAAATAT
CAATTTTCAAACATACTATT

Downstream 100 bases:

>100_bases
ACAATTATTGTTAATCTGGGCAATGTTATGCTCAGGAATTATTGCTTATTTTTGTTTAACAGACTCGAGTAATCTTCCTG
CAGTAAGTTTTCCCAGCATT

Product: glycine cleavage system protein H

Products: Proton; NADH; NH3; CO2; 5,10-methylene-THF [C]

Alternate protein names: NA

Number of amino acids: Translated: 126; Mature: 125

Protein sequence:

>126_residues
MSIPANLKYTKDHEWVSIEGDVATVGITHFAQKELGDIVYVEVETLDQTLDKDEVFGTVEAVKTVSDLFLPLTGEIIAFN
EDLESAPETVNSDPYGAGWMIKVKIANPSEIDSLLSDEAYKQLIGA

Sequences:

>Translated_126_residues
MSIPANLKYTKDHEWVSIEGDVATVGITHFAQKELGDIVYVEVETLDQTLDKDEVFGTVEAVKTVSDLFLPLTGEIIAFN
EDLESAPETVNSDPYGAGWMIKVKIANPSEIDSLLSDEAYKQLIGA
>Mature_125_residues
SIPANLKYTKDHEWVSIEGDVATVGITHFAQKELGDIVYVEVETLDQTLDKDEVFGTVEAVKTVSDLFLPLTGEIIAFNE
DLESAPETVNSDPYGAGWMIKVKIANPSEIDSLLSDEAYKQLIGA

Specific function: The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein [H]

COG id: COG0509

COG function: function code E; Glycine cleavage system H protein (lipoate-binding)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI49574537, Length=117, Percent_Identity=46.1538461538462, Blast_Score=121, Evalue=1e-28,
Organism=Homo sapiens, GI89057342, Length=117, Percent_Identity=45.2991452991453, Blast_Score=119, Evalue=9e-28,
Organism=Escherichia coli, GI1789271, Length=124, Percent_Identity=44.3548387096774, Blast_Score=115, Evalue=7e-28,
Organism=Caenorhabditis elegans, GI17507493, Length=115, Percent_Identity=39.1304347826087, Blast_Score=99, Evalue=6e-22,
Organism=Caenorhabditis elegans, GI17551294, Length=115, Percent_Identity=37.3913043478261, Blast_Score=99, Evalue=8e-22,
Organism=Saccharomyces cerevisiae, GI6319272, Length=125, Percent_Identity=36, Blast_Score=95, Evalue=3e-21,
Organism=Drosophila melanogaster, GI17865652, Length=120, Percent_Identity=36.6666666666667, Blast_Score=100, Evalue=3e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR002930
- InterPro:   IPR017453
- InterPro:   IPR011053 [H]

Pfam domain/function: PF01597 GCV_H [H]

EC number: NA

Molecular weight: Translated: 13805; Mature: 13673

Theoretical pI: Translated: 3.89; Mature: 3.89

Prosite motif: PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIPANLKYTKDHEWVSIEGDVATVGITHFAQKELGDIVYVEVETLDQTLDKDEVFGTVE
CCCCCCCEECCCCCEEEECCCEEEEHHHHHHHHHCCCEEEEEEEHHHHHCCHHHHHHHHH
AVKTVSDLFLPLTGEIIAFNEDLESAPETVNSDPYGAGWMIKVKIANPSEIDSLLSDEAY
HHHHHHHHHCCCCCEEEEECCCHHCCCCCCCCCCCCCCEEEEEEECCHHHHHHHHHHHHH
KQLIGA
HHHHCC
>Mature Secondary Structure 
SIPANLKYTKDHEWVSIEGDVATVGITHFAQKELGDIVYVEVETLDQTLDKDEVFGTVE
CCCCCCEECCCCCEEEECCCEEEEHHHHHHHHHCCCEEEEEEEHHHHHCCHHHHHHHHH
AVKTVSDLFLPLTGEIIAFNEDLESAPETVNSDPYGAGWMIKVKIANPSEIDSLLSDEAY
HHHHHHHHHCCCCCEEEEECCCHHCCCCCCCCCCCCCCEEEEEEECCHHHHHHHHHHHHH
KQLIGA
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Lipoyl Cofactor. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD; L-glycine; THF [C]

Specific reaction: NAD + L-glycine + THF = Proton + NADH + NH3 + CO2 + 5,10-methylene-THF [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA