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The map label for this gene is gltX

Identifier: 146298767

GI number: 146298767

Start: 1149303

End: 1150808

Strand: Direct

Name: gltX

Synonym: Fjoh_1006

Alternate gene names: 146298767

Gene position: 1149303-1150808 (Clockwise)

Preceding gene: 146298758

Following gene: 146298768

Centisome position: 18.85

GC content: 34.53

Gene sequence:

>1506_bases
ATGTCAAAGCAAGTTCGAGTGCGTTTTGCACCAAGTCCGACTGGACCATTACATATTGGCGGAGTTCGTACTGCCCTATT
TAATTATTTATTTGCAAAAAAACATAACGGTGTTTTTTATCTGAGAATTGAAGATACAGATCAGACACGTTTTGTTCCAG
GTGCCGAAGCTTATATTATGGAAGCACTGGAATGGTTAGGAATTTCTCCTGAAGAAACTGTTGGAAAAAATGAAAAATTT
GGTCCGTACAGACAAAGTGATCGTAAAGATTTATACCAAAAATATGCAGATCAATTGATCAATTCAGGCTGGGCGTATTA
TGCTTTTGATACTCCAGAAGCTCTTGATGCACATAGAAAACAACACGAAGCTGAAGGAAAAACATTTATTTATAATCATC
ACAACCGTGAAAAATTAGATACATCTTTAGTAATTTCTGCAGAAGAAACTGCTAAAAGAATTGCAAATGGAGAACATTAT
GTGATTCGTTTTAAAACTCCGGTTGATGAAACTTTACATTTAAAAGATATCATTCGTGGTGATGTTAAGTTTGAAACGAA
CTTGCTTGATGATAAAGTTCTTTTTAAAAGTGACGGAATGCCGACGTATCATTTAGCGAATATTGTGGATGATCATTTGA
TGGAAACTTCACATGTTATTCGTGGTGAAGAATGGTTACCATCTATGCCTCTTCACGTTTTATTATACAGAGCTTTTGGC
TGGGATGCTCCAGAATTTGCACATTTGCCTTTGATTTTAAAACCTGTTGGAAACGGAAAATTATCGAAAAGAGATGGTGA
TAAATTAGGATTTCCGGTGTTTCCATTAGAGTGGAAAACGGAAGAAGGCGTTTCATCTGGATACAGAGAGAAAGGATTTT
TCCCGGAAGCAGTTGTAAACTTCCTTGCTTTGTTAGGTTGGAATGATGGAACTGATAAAGAATTATTTTCTCTTGAAGAA
TTAGTTGAAGCTTTTGACTTAAACCGAGTTCATAAAGCAGGAGCAAAATTTGATCCTGAAAAAAACAAATGGTTCAATCA
CCAATATTTAATCAAACAAAATGATGCTGATTTAGCAAAGAGCTTCTCTACAATTTTAGAAGAAAAAGGCTTCTCTACTC
CGCTCGAAGTGACAACTCGTATTGTTTCTTTGATTAAAGAAAGAGCTCATTTCGTTTCTGAATTTTGGGATTTAACTGAT
TTCTTTTTTCAGGCACCATCATCTTATGATGAAAAAGCAAGTAAAAACTGGAAAGAAGAAACTCCAGCTTTAATGAAAGA
ATTGATTTCGGTTCTTGAAAATATTGAAGATTTTACTTCTGCAAACATTGAAACTATTGTAAAAGAATGGTTAACTAAAA
ACGAAATTGGAATGGGTAAAGTAATGCAGCCTTTCCGTTTAAGTTTGGTTGGAGCTCTTAAAGGTCCTCACCTATTTGAC
ATTGTAGAAATCATTGGAAAAGAAGAAACTGTTTCAAGAATTCAGAAAGCAATTTCTGCTTTATAA

Upstream 100 bases:

>100_bases
AGTCGAAAGTCAAAAGTTTAGCACACAGCTAAAAACCTTTCAAACTTTCGACTTTTGGCTTTTGACTAATATCGTATCTT
TGCATCAAAATTTAAACAAA

Downstream 100 bases:

>100_bases
AAATATTTTACCATTAAGATATTAAGTTAATTAAGCATTTCTTGGTGGTAAAAAACAGAACTTAAAAGTTCATAAAGCTA
AGCTTAATTCTCTTGACAAA

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase; GluRS

Number of amino acids: Translated: 501; Mature: 500

Protein sequence:

>501_residues
MSKQVRVRFAPSPTGPLHIGGVRTALFNYLFAKKHNGVFYLRIEDTDQTRFVPGAEAYIMEALEWLGISPEETVGKNEKF
GPYRQSDRKDLYQKYADQLINSGWAYYAFDTPEALDAHRKQHEAEGKTFIYNHHNREKLDTSLVISAEETAKRIANGEHY
VIRFKTPVDETLHLKDIIRGDVKFETNLLDDKVLFKSDGMPTYHLANIVDDHLMETSHVIRGEEWLPSMPLHVLLYRAFG
WDAPEFAHLPLILKPVGNGKLSKRDGDKLGFPVFPLEWKTEEGVSSGYREKGFFPEAVVNFLALLGWNDGTDKELFSLEE
LVEAFDLNRVHKAGAKFDPEKNKWFNHQYLIKQNDADLAKSFSTILEEKGFSTPLEVTTRIVSLIKERAHFVSEFWDLTD
FFFQAPSSYDEKASKNWKEETPALMKELISVLENIEDFTSANIETIVKEWLTKNEIGMGKVMQPFRLSLVGALKGPHLFD
IVEIIGKEETVSRIQKAISAL

Sequences:

>Translated_501_residues
MSKQVRVRFAPSPTGPLHIGGVRTALFNYLFAKKHNGVFYLRIEDTDQTRFVPGAEAYIMEALEWLGISPEETVGKNEKF
GPYRQSDRKDLYQKYADQLINSGWAYYAFDTPEALDAHRKQHEAEGKTFIYNHHNREKLDTSLVISAEETAKRIANGEHY
VIRFKTPVDETLHLKDIIRGDVKFETNLLDDKVLFKSDGMPTYHLANIVDDHLMETSHVIRGEEWLPSMPLHVLLYRAFG
WDAPEFAHLPLILKPVGNGKLSKRDGDKLGFPVFPLEWKTEEGVSSGYREKGFFPEAVVNFLALLGWNDGTDKELFSLEE
LVEAFDLNRVHKAGAKFDPEKNKWFNHQYLIKQNDADLAKSFSTILEEKGFSTPLEVTTRIVSLIKERAHFVSEFWDLTD
FFFQAPSSYDEKASKNWKEETPALMKELISVLENIEDFTSANIETIVKEWLTKNEIGMGKVMQPFRLSLVGALKGPHLFD
IVEIIGKEETVSRIQKAISAL
>Mature_500_residues
SKQVRVRFAPSPTGPLHIGGVRTALFNYLFAKKHNGVFYLRIEDTDQTRFVPGAEAYIMEALEWLGISPEETVGKNEKFG
PYRQSDRKDLYQKYADQLINSGWAYYAFDTPEALDAHRKQHEAEGKTFIYNHHNREKLDTSLVISAEETAKRIANGEHYV
IRFKTPVDETLHLKDIIRGDVKFETNLLDDKVLFKSDGMPTYHLANIVDDHLMETSHVIRGEEWLPSMPLHVLLYRAFGW
DAPEFAHLPLILKPVGNGKLSKRDGDKLGFPVFPLEWKTEEGVSSGYREKGFFPEAVVNFLALLGWNDGTDKELFSLEEL
VEAFDLNRVHKAGAKFDPEKNKWFNHQYLIKQNDADLAKSFSTILEEKGFSTPLEVTTRIVSLIKERAHFVSEFWDLTDF
FFQAPSSYDEKASKNWKEETPALMKELISVLENIEDFTSANIETIVKEWLTKNEIGMGKVMQPFRLSLVGALKGPHLFDI
VEIIGKEETVSRIQKAISAL

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu)

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI134288884, Length=520, Percent_Identity=34.6153846153846, Blast_Score=268, Evalue=1e-71,
Organism=Homo sapiens, GI4826960, Length=316, Percent_Identity=25, Blast_Score=75, Evalue=2e-13,
Organism=Homo sapiens, GI62241042, Length=306, Percent_Identity=24.8366013071895, Blast_Score=66, Evalue=1e-10,
Organism=Escherichia coli, GI1788743, Length=500, Percent_Identity=34.2, Blast_Score=275, Evalue=4e-75,
Organism=Escherichia coli, GI226510925, Length=258, Percent_Identity=34.4961240310078, Blast_Score=120, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI71993394, Length=505, Percent_Identity=38.2178217821782, Blast_Score=294, Evalue=7e-80,
Organism=Saccharomyces cerevisiae, GI6324540, Length=520, Percent_Identity=36.9230769230769, Blast_Score=296, Evalue=5e-81,
Organism=Saccharomyces cerevisiae, GI6324742, Length=315, Percent_Identity=25.0793650793651, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24665321, Length=513, Percent_Identity=35.2826510721248, Blast_Score=282, Evalue=4e-76,
Organism=Drosophila melanogaster, GI17864488, Length=309, Percent_Identity=25.2427184466019, Blast_Score=72, Evalue=1e-12,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYE_FLAJ1 (A5FL73)

Other databases:

- EMBL:   CP000685
- RefSeq:   YP_001193358.1
- ProteinModelPortal:   A5FL73
- SMR:   A5FL73
- STRING:   A5FL73
- GeneID:   5090597
- GenomeReviews:   CP000685_GR
- KEGG:   fjo:Fjoh_1006
- eggNOG:   COG0008
- HOGENOM:   HBG628189
- OMA:   MAHIPLI
- ProtClustDB:   PRK01406
- BioCyc:   FJOH376686:FJOH_1006-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00022_B
- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729
- Gene3D:   G3DSA:1.10.1160.10
- Gene3D:   G3DSA:3.40.50.620
- Gene3D:   G3DSA:1.10.10.350
- PANTHER:   PTHR10119
- PRINTS:   PR00987
- TIGRFAMs:   TIGR00464

Pfam domain/function: PF00749 tRNA-synt_1c; SSF48163 tRNA-synt_bind

EC number: =6.1.1.17

Molecular weight: Translated: 57366; Mature: 57235

Theoretical pI: Translated: 5.86; Mature: 5.86

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 263-263

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKQVRVRFAPSPTGPLHIGGVRTALFNYLFAKKHNGVFYLRIEDTDQTRFVPGAEAYIM
CCCCEEEEECCCCCCCEEECHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCHHHHHH
EALEWLGISPEETVGKNEKFGPYRQSDRKDLYQKYADQLINSGWAYYAFDTPEALDAHRK
HHHHHCCCCCHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHH
QHEAEGKTFIYNHHNREKLDTSLVISAEETAKRIANGEHYVIRFKTPVDETLHLKDIIRG
HHHCCCCEEEEECCCCHHCCCEEEEECHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHCC
DVKFETNLLDDKVLFKSDGMPTYHLANIVDDHLMETSHVIRGEEWLPSMPLHVLLYRAFG
CCEEECCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHC
WDAPEFAHLPLILKPVGNGKLSKRDGDKLGFPVFPLEWKTEEGVSSGYREKGFFPEAVVN
CCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCHHCCCCHHHHHH
FLALLGWNDGTDKELFSLEELVEAFDLNRVHKAGAKFDPEKNKWFNHQYLIKQNDADLAK
HHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCHHHHH
SFSTILEEKGFSTPLEVTTRIVSLIKERAHFVSEFWDLTDFFFQAPSSYDEKASKNWKEE
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCHHHC
TPALMKELISVLENIEDFTSANIETIVKEWLTKNEIGMGKVMQPFRLSLVGALKGPHLFD
CHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCHHCHHHHHHHHHHCCCCHHHH
IVEIIGKEETVSRIQKAISAL
HHHHHCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
SKQVRVRFAPSPTGPLHIGGVRTALFNYLFAKKHNGVFYLRIEDTDQTRFVPGAEAYIM
CCCEEEEECCCCCCCEEECHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCHHHHHH
EALEWLGISPEETVGKNEKFGPYRQSDRKDLYQKYADQLINSGWAYYAFDTPEALDAHRK
HHHHHCCCCCHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHH
QHEAEGKTFIYNHHNREKLDTSLVISAEETAKRIANGEHYVIRFKTPVDETLHLKDIIRG
HHHCCCCEEEEECCCCHHCCCEEEEECHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHCC
DVKFETNLLDDKVLFKSDGMPTYHLANIVDDHLMETSHVIRGEEWLPSMPLHVLLYRAFG
CCEEECCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHC
WDAPEFAHLPLILKPVGNGKLSKRDGDKLGFPVFPLEWKTEEGVSSGYREKGFFPEAVVN
CCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCHHCCCCHHHHHH
FLALLGWNDGTDKELFSLEELVEAFDLNRVHKAGAKFDPEKNKWFNHQYLIKQNDADLAK
HHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCHHHHH
SFSTILEEKGFSTPLEVTTRIVSLIKERAHFVSEFWDLTDFFFQAPSSYDEKASKNWKEE
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCHHHC
TPALMKELISVLENIEDFTSANIETIVKEWLTKNEIGMGKVMQPFRLSLVGALKGPHLFD
CHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCHHCHHHHHHHHHHCCCCHHHH
IVEIIGKEETVSRIQKAISAL
HHHHHCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA