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The map label for this gene is hemB [H]

Identifier: 146298719

GI number: 146298719

Start: 1068872

End: 1069867

Strand: Direct

Name: hemB [H]

Synonym: Fjoh_0957

Alternate gene names: 146298719

Gene position: 1068872-1069867 (Clockwise)

Preceding gene: 146298715

Following gene: 146298720

Centisome position: 17.53

GC content: 38.05

Gene sequence:

>996_bases
ATGTTCCCATTACAAAGAGGCAGAAGATTAAGAGTAAATGAGTCTATTCGTTCTTTAGTACGCGAAACCAGTTTAAGTCC
GTCGGATTTTATGTTTCCAATGTTTATTGCTGAAGGAGAAAATGTAAAAGTCGAAATTCCGTCAATGCCGGGAATTTTCA
GAAGATCAATAGATTTAACAGTTGAAGAAGTAAAAGAACTTTACGATTTAGGAATTCGCGCCGTAAATATTTATGTAAAA
GTCAGCGAAAACCTAAAAGACAATACTGGAAAAGAAGCATGGAATTCTAACGGATTAATGCAGCAAGCTATTCGTGCCAT
AAAAGCCGCTTGTCCGGAAATGATCGTTATGCCAGATGTCGCATTAGACCCATACTCTATTTACGGTCATGACGGAATTA
TAACAAATGGTGATGTAGAAAACGACAGTACAGTTGATGCTTTAGTAAAAATGGCTGTTTCACATGCAGAAGCTGGTGCC
GATTTTGTTGCGCCAAGTGATATGATGGACGGACGTGTTTTACGCCTGCGCGAAGGTTTAGACGCTGCCGGTTTCCAAAA
TGTGGGAATCATGAGTTATTCGGCTAAATATGCTTCAGCTTTTTATGGTCCTTTTAGAGATGCTTTAGATTCGGCTCCAA
AAGAAGCGGATGTTGTGGTTCCAAAAGACAAAAAAACATATCAAATGGATTATGCTAATCGTATTGAAGCGATTAAAGAA
GCTTTGTCTGACGTAGAAGAAGGTGCAGACATGGTAATGGTAAAACCCGGAATTGCTTATTTGGATATTGTTCGCGAAAT
AAAAAACACGGTACATGTTCCTGTAACTGTTTATCAGGTTTCTGGAGAATATGCCATGATTAAAGCCGCATCTGAAAGAG
GCTGGCTTGATCACGATAAAATTATGATGGAACAATTAATGTGCATTAAACGTTCTGGTGCTAATCTTATTTCGACATAC
TTCGCAAAAGAAGCTGCAATTTTATTGAATAAATAA

Upstream 100 bases:

>100_bases
AAAATAAAAATAACTGCTGTTTTAGAAATAGATTCTAAGTCTGAGTTTCAAAGAAATCAACTAAAAAATCTAAAATCTAA
AATCTAAAATCTAAAATTAT

Downstream 100 bases:

>100_bases
TATGAAAAAAATAGTATTCTTATCTGCCGTTTTAGCTTTTTCGTCTTGCAAAAAAGAAGTTTCAGAAAATACCGATTCCA
AAACAGAAACCTATTCTGAA

Product: delta-aminolevulinic acid dehydratase

Products: NA

Alternate protein names: ALAD; ALADH; Porphobilinogen synthase [H]

Number of amino acids: Translated: 331; Mature: 331

Protein sequence:

>331_residues
MFPLQRGRRLRVNESIRSLVRETSLSPSDFMFPMFIAEGENVKVEIPSMPGIFRRSIDLTVEEVKELYDLGIRAVNIYVK
VSENLKDNTGKEAWNSNGLMQQAIRAIKAACPEMIVMPDVALDPYSIYGHDGIITNGDVENDSTVDALVKMAVSHAEAGA
DFVAPSDMMDGRVLRLREGLDAAGFQNVGIMSYSAKYASAFYGPFRDALDSAPKEADVVVPKDKKTYQMDYANRIEAIKE
ALSDVEEGADMVMVKPGIAYLDIVREIKNTVHVPVTVYQVSGEYAMIKAASERGWLDHDKIMMEQLMCIKRSGANLISTY
FAKEAAILLNK

Sequences:

>Translated_331_residues
MFPLQRGRRLRVNESIRSLVRETSLSPSDFMFPMFIAEGENVKVEIPSMPGIFRRSIDLTVEEVKELYDLGIRAVNIYVK
VSENLKDNTGKEAWNSNGLMQQAIRAIKAACPEMIVMPDVALDPYSIYGHDGIITNGDVENDSTVDALVKMAVSHAEAGA
DFVAPSDMMDGRVLRLREGLDAAGFQNVGIMSYSAKYASAFYGPFRDALDSAPKEADVVVPKDKKTYQMDYANRIEAIKE
ALSDVEEGADMVMVKPGIAYLDIVREIKNTVHVPVTVYQVSGEYAMIKAASERGWLDHDKIMMEQLMCIKRSGANLISTY
FAKEAAILLNK
>Mature_331_residues
MFPLQRGRRLRVNESIRSLVRETSLSPSDFMFPMFIAEGENVKVEIPSMPGIFRRSIDLTVEEVKELYDLGIRAVNIYVK
VSENLKDNTGKEAWNSNGLMQQAIRAIKAACPEMIVMPDVALDPYSIYGHDGIITNGDVENDSTVDALVKMAVSHAEAGA
DFVAPSDMMDGRVLRLREGLDAAGFQNVGIMSYSAKYASAFYGPFRDALDSAPKEADVVVPKDKKTYQMDYANRIEAIKE
ALSDVEEGADMVMVKPGIAYLDIVREIKNTVHVPVTVYQVSGEYAMIKAASERGWLDHDKIMMEQLMCIKRSGANLISTY
FAKEAAILLNK

Specific function: Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen [H]

COG id: COG0113

COG function: function code H; Delta-aminolevulinic acid dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ALADH family [H]

Homologues:

Organism=Homo sapiens, GI189083849, Length=305, Percent_Identity=38.6885245901639, Blast_Score=214, Evalue=1e-55,
Organism=Escherichia coli, GI87081728, Length=323, Percent_Identity=45.8204334365325, Blast_Score=273, Evalue=1e-74,
Organism=Saccharomyces cerevisiae, GI6321398, Length=313, Percent_Identity=37.0607028753994, Blast_Score=194, Evalue=2e-50,
Organism=Drosophila melanogaster, GI21358291, Length=300, Percent_Identity=36.3333333333333, Blast_Score=206, Evalue=2e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001731
- InterPro:   IPR013785 [H]

Pfam domain/function: PF00490 ALAD [H]

EC number: =4.2.1.24 [H]

Molecular weight: Translated: 36753; Mature: 36753

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: PS00169 D_ALA_DEHYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
5.4 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
5.4 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFPLQRGRRLRVNESIRSLVRETSLSPSDFMFPMFIAEGENVKVEIPSMPGIFRRSIDLT
CCCCCCCCEEEECHHHHHHHHHHCCCCCCCEEEEEEECCCEEEEECCCCCCHHHHHHCCC
VEEVKELYDLGIRAVNIYVKVSENLKDNTGKEAWNSNGLMQQAIRAIKAACPEMIVMPDV
HHHHHHHHHCCCEEEEEEEEECCCCCCCCCCHHHCCCCHHHHHHHHHHHCCCCEEEECCC
ALDPYSIYGHDGIITNGDVENDSTVDALVKMAVSHAEAGADFVAPSDMMDGRVLRLREGL
CCCCEEEECCCCEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHCCC
DAAGFQNVGIMSYSAKYASAFYGPFRDALDSAPKEADVVVPKDKKTYQMDYANRIEAIKE
CCCCCCCCCEEEECHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCEEEHHHHHHHHHHHH
ALSDVEEGADMVMVKPGIAYLDIVREIKNTVHVPVTVYQVSGEYAMIKAASERGWLDHDK
HHHHHHCCCCEEEECCCHHHHHHHHHHHCCEECEEEEEEECCCEEEEEECCCCCCCCHHH
IMMEQLMCIKRSGANLISTYFAKEAAILLNK
HHHHHHHHHHHCCCHHHHHHHHHHHHHEECC
>Mature Secondary Structure
MFPLQRGRRLRVNESIRSLVRETSLSPSDFMFPMFIAEGENVKVEIPSMPGIFRRSIDLT
CCCCCCCCEEEECHHHHHHHHHHCCCCCCCEEEEEEECCCEEEEECCCCCCHHHHHHCCC
VEEVKELYDLGIRAVNIYVKVSENLKDNTGKEAWNSNGLMQQAIRAIKAACPEMIVMPDV
HHHHHHHHHCCCEEEEEEEEECCCCCCCCCCHHHCCCCHHHHHHHHHHHCCCCEEEECCC
ALDPYSIYGHDGIITNGDVENDSTVDALVKMAVSHAEAGADFVAPSDMMDGRVLRLREGL
CCCCEEEECCCCEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHCCC
DAAGFQNVGIMSYSAKYASAFYGPFRDALDSAPKEADVVVPKDKKTYQMDYANRIEAIKE
CCCCCCCCCEEEECHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCEEEHHHHHHHHHHHH
ALSDVEEGADMVMVKPGIAYLDIVREIKNTVHVPVTVYQVSGEYAMIKAASERGWLDHDK
HHHHHHCCCCEEEECCCHHHHHHHHHHHCCEECEEEEEEECCCEEEEEECCCCCCCCHHH
IMMEQLMCIKRSGANLISTYFAKEAAILLNK
HHHHHHHHHHHCCCHHHHHHHHHHHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8626508 [H]