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The map label for this gene is bioB

Identifier: 146298563

GI number: 146298563

Start: 893929

End: 895020

Strand: Direct

Name: bioB

Synonym: Fjoh_0801

Alternate gene names: 146298563

Gene position: 893929-895020 (Clockwise)

Preceding gene: 146298562

Following gene: 146298564

Centisome position: 14.66

GC content: 40.38

Gene sequence:

>1092_bases
ATGAGCATGACTACAAAACACAATTGGACAAAAGACGAAATTATTGCAATCTACAACAAACCATTAATGGATTTGCTTTA
TGAGGCAGCGACGATTCACAGACAACAACATGATCCAAACGTGGTTCAGGTATCTACCCTGCTTTCTATAAAAACCGGCG
GATGTCCGGAAGATTGCGGATACTGCCCACAAGCGGCTAGATATAATACAGGAGTTGAAGGAAATGATTTAATGACTGTA
AGTCAGGTAAAAGCACAGGCATTAAGAGCAAAATCAAACGGATCATCCCGTGTATGCATGGGTGCTGCCTGGAGAAATGT
TAAAGATGGTGAAGAATTTGATCAGGTTTTAGAAATGGTACGAACCATTAATAAACTTGATATGGAAGTCTGCTGCACTT
TAGGAATGCTGACCGAGAATCAGGCACAGCGTTTAGCCGAAGCCGGTTTGTATGCTTACAACCATAATTTAGATACTTCT
GAGGAATACTATAAAGATGTAATTTCAACACGCGGTTTTGAAGACCGTTTACAAACTATAGAAAATGTTCGTAAAACGAA
TGTAACAGTTTGCAGCGGCGGTATTATTGGAATGGGAGAAAGTATTGAAGACAGAGCCGGAATGCTTGTTGCACTATCAA
CTTTAAATCCACAGCCAGAATCCGTTCCTATTAATGCCTTAGTTGCTGTTGAAGGAACTCCAATGGAAGAAGAAAAACCG
GTTGAGATCTGGGAGATGATTCGTATGGTAGCTACTACCCGAATTGTAATGCCGGAAACTCAGGTTCGCTTGTCTGCCGG
AAGAACCAATATGTCGCGCGAAGGTCAGGCAATGTGCTTTTTTGCCGGAGCGAATTCAATTTTTGCCGGAGATAAGCTAC
TTACTACACCAAATCCTGATGTTAACGAAGACATGAAGATGTTTGAGACTTTAGGCATGGTGGCTCAAAAACCATTTATT
AAAATAATGCAGCCTAAAACAGTTGAAGCTGCTGATTCTCAATTTGCTCCATTAGGAGAAAAACCAAAATGGTCTAGACC
CGGACATACAATTGAAAGAAACATTGAAGCATCGATCAAATCAAAAATTTAA

Upstream 100 bases:

>100_bases
GCTTTTTTTATGAAATTATTTAACAAATATTCATTTACAACACGCAATGTTGTTGATTCAAAAATTTAATTACATTTGAA
GCTTCAAAAACAATTTATCC

Downstream 100 bases:

>100_bases
TTAAAAGAGCTCAAAAATCTCGATAAATATTTTTAAATTGCTTATGACACAGAGTTATAAGCAATTTTTTATTTGTACTA
ATGAAATTAAAACAAATCGA

Product: biotin synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 363; Mature: 362

Protein sequence:

>363_residues
MSMTTKHNWTKDEIIAIYNKPLMDLLYEAATIHRQQHDPNVVQVSTLLSIKTGGCPEDCGYCPQAARYNTGVEGNDLMTV
SQVKAQALRAKSNGSSRVCMGAAWRNVKDGEEFDQVLEMVRTINKLDMEVCCTLGMLTENQAQRLAEAGLYAYNHNLDTS
EEYYKDVISTRGFEDRLQTIENVRKTNVTVCSGGIIGMGESIEDRAGMLVALSTLNPQPESVPINALVAVEGTPMEEEKP
VEIWEMIRMVATTRIVMPETQVRLSAGRTNMSREGQAMCFFAGANSIFAGDKLLTTPNPDVNEDMKMFETLGMVAQKPFI
KIMQPKTVEAADSQFAPLGEKPKWSRPGHTIERNIEASIKSKI

Sequences:

>Translated_363_residues
MSMTTKHNWTKDEIIAIYNKPLMDLLYEAATIHRQQHDPNVVQVSTLLSIKTGGCPEDCGYCPQAARYNTGVEGNDLMTV
SQVKAQALRAKSNGSSRVCMGAAWRNVKDGEEFDQVLEMVRTINKLDMEVCCTLGMLTENQAQRLAEAGLYAYNHNLDTS
EEYYKDVISTRGFEDRLQTIENVRKTNVTVCSGGIIGMGESIEDRAGMLVALSTLNPQPESVPINALVAVEGTPMEEEKP
VEIWEMIRMVATTRIVMPETQVRLSAGRTNMSREGQAMCFFAGANSIFAGDKLLTTPNPDVNEDMKMFETLGMVAQKPFI
KIMQPKTVEAADSQFAPLGEKPKWSRPGHTIERNIEASIKSKI
>Mature_362_residues
SMTTKHNWTKDEIIAIYNKPLMDLLYEAATIHRQQHDPNVVQVSTLLSIKTGGCPEDCGYCPQAARYNTGVEGNDLMTVS
QVKAQALRAKSNGSSRVCMGAAWRNVKDGEEFDQVLEMVRTINKLDMEVCCTLGMLTENQAQRLAEAGLYAYNHNLDTSE
EYYKDVISTRGFEDRLQTIENVRKTNVTVCSGGIIGMGESIEDRAGMLVALSTLNPQPESVPINALVAVEGTPMEEEKPV
EIWEMIRMVATTRIVMPETQVRLSAGRTNMSREGQAMCFFAGANSIFAGDKLLTTPNPDVNEDMKMFETLGMVAQKPFIK
IMQPKTVEAADSQFAPLGEKPKWSRPGHTIERNIEASIKSKI

Specific function: Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical- based mechanism

COG id: COG0502

COG function: function code H; Biotin synthase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Biotin synthase family

Homologues:

Organism=Escherichia coli, GI1786992, Length=315, Percent_Identity=53.3333333333333, Blast_Score=366, Evalue=1e-102,
Organism=Saccharomyces cerevisiae, GI6321725, Length=320, Percent_Identity=46.875, Blast_Score=325, Evalue=9e-90,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BIOB_FLAJ1 (A5FLT1)

Other databases:

- EMBL:   CP000685
- RefSeq:   YP_001193154.1
- ProteinModelPortal:   A5FLT1
- SMR:   A5FLT1
- STRING:   A5FLT1
- GeneID:   5091990
- GenomeReviews:   CP000685_GR
- KEGG:   fjo:Fjoh_0801
- eggNOG:   COG0502
- HOGENOM:   HBG405569
- OMA:   MGMETCM
- ProtClustDB:   CLSK2298548
- BioCyc:   FJOH376686:FJOH_0801-MONOMER
- HAMAP:   MF_01694
- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR002684
- InterPro:   IPR006638
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00876
- SMART:   SM00729
- TIGRFAMs:   TIGR00433

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM

EC number: =2.8.1.6

Molecular weight: Translated: 40268; Mature: 40136

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
5.5 %Met     (Translated Protein)
7.7 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
5.2 %Met     (Mature Protein)
7.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSMTTKHNWTKDEIIAIYNKPLMDLLYEAATIHRQQHDPNVVQVSTLLSIKTGGCPEDCG
CCCCCCCCCCHHHEEEEECCHHHHHHHHHHHHHHHCCCCCEEEEEEEEEECCCCCCHHCC
YCPQAARYNTGVEGNDLMTVSQVKAQALRAKSNGSSRVCMGAAWRNVKDGEEFDQVLEMV
CCCCCCCCCCCCCCCCEEHHHHHHHHHHHHCCCCCCEEEEHHHHCCCCCHHHHHHHHHHH
RTINKLDMEVCCTLGMLTENQAQRLAEAGLYAYNHNLDTSEEYYKDVISTRGFEDRLQTI
HHHHHHHHHHHHHHHHHCHHHHHHHHHHCCEEEECCCCCHHHHHHHHHHCCCHHHHHHHH
ENVRKTNVTVCSGGIIGMGESIEDRAGMLVALSTLNPQPESVPINALVAVEGTPMEEEKP
HHHHHCCEEEECCCEEECCCCCCCCCCEEEEEECCCCCCCCCCCEEEEEECCCCCCCCCC
VEIWEMIRMVATTRIVMPETQVRLSAGRTNMSREGQAMCFFAGANSIFAGDKLLTTPNPD
HHHHHHHHHHHHHHEECCCCCEEEECCCCCCCCCCCEEEEEECCCCEECCCEEEECCCCC
VNEDMKMFETLGMVAQKPFIKIMQPKTVEAADSQFAPLGEKPKWSRPGHTIERNIEASIK
CCHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCHHHHHH
SKI
CCC
>Mature Secondary Structure 
SMTTKHNWTKDEIIAIYNKPLMDLLYEAATIHRQQHDPNVVQVSTLLSIKTGGCPEDCG
CCCCCCCCCHHHEEEEECCHHHHHHHHHHHHHHHCCCCCEEEEEEEEEECCCCCCHHCC
YCPQAARYNTGVEGNDLMTVSQVKAQALRAKSNGSSRVCMGAAWRNVKDGEEFDQVLEMV
CCCCCCCCCCCCCCCCEEHHHHHHHHHHHHCCCCCCEEEEHHHHCCCCCHHHHHHHHHHH
RTINKLDMEVCCTLGMLTENQAQRLAEAGLYAYNHNLDTSEEYYKDVISTRGFEDRLQTI
HHHHHHHHHHHHHHHHHCHHHHHHHHHHCCEEEECCCCCHHHHHHHHHHCCCHHHHHHHH
ENVRKTNVTVCSGGIIGMGESIEDRAGMLVALSTLNPQPESVPINALVAVEGTPMEEEKP
HHHHHCCEEEECCCEEECCCCCCCCCCEEEEEECCCCCCCCCCCEEEEEECCCCCCCCCC
VEIWEMIRMVATTRIVMPETQVRLSAGRTNMSREGQAMCFFAGANSIFAGDKLLTTPNPD
HHHHHHHHHHHHHHEECCCCCEEEECCCCCCCCCCCEEEEEECCCCEECCCEEEECCCCC
VNEDMKMFETLGMVAQKPFIKIMQPKTVEAADSQFAPLGEKPKWSRPGHTIERNIEASIK
CCHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCHHHHHH
SKI
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA