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The map label for this gene is ushA [H]

Identifier: 146284451

GI number: 146284451

Start: 4492226

End: 4494043

Strand: Direct

Name: ushA [H]

Synonym: PST_4140

Alternate gene names: 146284451

Gene position: 4492226-4494043 (Clockwise)

Preceding gene: 146284449

Following gene: 146284456

Centisome position: 98.35

GC content: 68.32

Gene sequence:

>1818_bases
ATGCGTATGCGCGTATTGGATTGGTTGTTCGCCGCAGGCCTGATCGGCCTTGCCGCCCCAACGCTGGCCCAGACCCAGAA
TCAGCCCGAGCACGGTTCCACCGAGCAGGACTACCGCGAGGAGCGCCAGGAAGGACTGCAGGCCCGGCAGCAGGCCAGCG
AGCGACGCCGCTGGCGCGCACCGAATTGGCGCCCCGAGCCGGTGGTGTCGGTGAACATCCTCGGCATCAACGACTTCCAT
GGTCAGCTGTCGCCGCGCACCGTTGCCGGGCGGCCGGCAGGCGGCGCGGCAGTGCTGGCGTCCTACCTCAGATCGGCCAG
CCGCGAGTACACCCTCATCGTTCACGACGGCGACCAGGTCGGCGCCTCGCCACCCAACTCCGCCCTGCTGCGCGACGAAC
CTGCGATCAGCCTGCTCAATCTGCTGGCCAATCCGTTCTGCCGTCCGTTCAGCTGGCAGATGAAGCTACCCCGCAGCGCC
CAGCCTCTCGCCCAGCAGCGCTGCAACGTGGTCGGCACCCTTGGCAACCACGAGTTCGACTACGGCAAGAATGAGCTGCT
GCGCCAGCTCACCGGTGGCAACCATGCCAATGGTCCCTACCTCGAAGAGAACTGGCAGGGGGCGCGCTACCCCACGGTGT
CGAGCAACGTCATCAATCAGTCGAGCGGCCGATCGCTGTTGCCGCCGCACACCATCTACAACGCCGGCGGCGTACGCATC
GGCGTGATCGGCGCCGCGCTGAAGGAAACCCCCTCCATCGTCTCGCCCAGCGGCGTGGCCGGCCTGCGCTTCATCGACGA
AGCGGCCGCCATCAACCGTTCGGTGGCACAACTGCGCCGCCAGGGCGTGCGCGCCATCATCGTCGCGCTGCACCAGGGCG
GCCAGCAGACCAGCTACAACGGCCCGACCAACGCCGAGGCGGACACCGTGGTCGGCCCCGTGGTCGATATCGTCAAGCGC
CTGGATGACGAGGTGGACGTGGTCATCTCCGGCCATGCCCACGGCTTCACCAACGCGCTGCTACCCAACGCCAACGGCAA
GCCGATTCTGGTGACCCAGGCCTTCTCGGCGGGCACCGCCTATGCCGACATCGAGCTGCTGGTCAGCCGCCGCAGCCGCG
ACGTCGTCGAAAAATCCGCTGCGATCCTCACCACCTGGGCCGACCAGGGCGCAGGCCTCACGCCGGACCCGCAAGTCGCC
GCGCTGGTGGCCCAGGCCGATGCCCGCGTCGAGCCGCTGGTGGCGCGGGTGGTCGGCCTGGCGCAAGGGGCCATCACCCG
CACCGAGACACCCGCCGGTGAATCCGCACTGGGCAACCTGATTGCCGACGCCCAGCGCGCGGCCACCGGGGCGCAGATCA
GCTTCATGAACCCGGGCGGCATCCGCGCGGACCTCGACGGCGGCGAGGTCACCTGGGGCGAACTGTTCGCCATCCAGCCG
TTCGCCAACGACCTGGTCTCGATGGACCTCACCGGCGCGCAGATCAAGACCCTGCTCGAACAGCAGTGGATCGGCCAGAG
CTACCCGCGTCTGCTCAAGCCTTCCGGCATCCTGTACAGCTGGGCCGCGAACCGCCCCGAGGGCAACCGCGTGATCGAAA
TGCGCGACGCCAGCGGCGCCCCCCTCAACCCGGCGGCAACCTATCGCGTCACGGTGAACTCCTTCCTGGCCGGCGGCGGC
GACAACTTCACCATCCTCAACGAAGGCCAGAACCGCGTCGTCGGCCCGGTGGATCTGGATGCGCTGGTGGGCTACATCGA
AGCCCTGCCGCAACCGTTCAGCGCCAGCGTCGAGGGGCGTATCCAGCGGGTGGATTGA

Upstream 100 bases:

>100_bases
GTCGGCGCTCGTCATAACGCAAACCGGCCGTCATCAACCTGCCACCTGAGCTTCCTAGTTTCGGTCCCTGCCCATCCCAC
CGCACCCGAGGAAAACAACA

Downstream 100 bases:

>100_bases
AGCCGGAACAAACGAGGCCGACACCCCGTAGGGCCGGGCGTCAGCCCACCCGCCATCCCCCGCGTAGCTGCAACGAGCTA
CAGCGCCATCGCCGCGTGAC

Product: 5'-nucleotidase

Products: Phosphate; Uridine [C]

Alternate protein names: NA

Number of amino acids: Translated: 605; Mature: 605

Protein sequence:

>605_residues
MRMRVLDWLFAAGLIGLAAPTLAQTQNQPEHGSTEQDYREERQEGLQARQQASERRRWRAPNWRPEPVVSVNILGINDFH
GQLSPRTVAGRPAGGAAVLASYLRSASREYTLIVHDGDQVGASPPNSALLRDEPAISLLNLLANPFCRPFSWQMKLPRSA
QPLAQQRCNVVGTLGNHEFDYGKNELLRQLTGGNHANGPYLEENWQGARYPTVSSNVINQSSGRSLLPPHTIYNAGGVRI
GVIGAALKETPSIVSPSGVAGLRFIDEAAAINRSVAQLRRQGVRAIIVALHQGGQQTSYNGPTNAEADTVVGPVVDIVKR
LDDEVDVVISGHAHGFTNALLPNANGKPILVTQAFSAGTAYADIELLVSRRSRDVVEKSAAILTTWADQGAGLTPDPQVA
ALVAQADARVEPLVARVVGLAQGAITRTETPAGESALGNLIADAQRAATGAQISFMNPGGIRADLDGGEVTWGELFAIQP
FANDLVSMDLTGAQIKTLLEQQWIGQSYPRLLKPSGILYSWAANRPEGNRVIEMRDASGAPLNPAATYRVTVNSFLAGGG
DNFTILNEGQNRVVGPVDLDALVGYIEALPQPFSASVEGRIQRVD

Sequences:

>Translated_605_residues
MRMRVLDWLFAAGLIGLAAPTLAQTQNQPEHGSTEQDYREERQEGLQARQQASERRRWRAPNWRPEPVVSVNILGINDFH
GQLSPRTVAGRPAGGAAVLASYLRSASREYTLIVHDGDQVGASPPNSALLRDEPAISLLNLLANPFCRPFSWQMKLPRSA
QPLAQQRCNVVGTLGNHEFDYGKNELLRQLTGGNHANGPYLEENWQGARYPTVSSNVINQSSGRSLLPPHTIYNAGGVRI
GVIGAALKETPSIVSPSGVAGLRFIDEAAAINRSVAQLRRQGVRAIIVALHQGGQQTSYNGPTNAEADTVVGPVVDIVKR
LDDEVDVVISGHAHGFTNALLPNANGKPILVTQAFSAGTAYADIELLVSRRSRDVVEKSAAILTTWADQGAGLTPDPQVA
ALVAQADARVEPLVARVVGLAQGAITRTETPAGESALGNLIADAQRAATGAQISFMNPGGIRADLDGGEVTWGELFAIQP
FANDLVSMDLTGAQIKTLLEQQWIGQSYPRLLKPSGILYSWAANRPEGNRVIEMRDASGAPLNPAATYRVTVNSFLAGGG
DNFTILNEGQNRVVGPVDLDALVGYIEALPQPFSASVEGRIQRVD
>Mature_605_residues
MRMRVLDWLFAAGLIGLAAPTLAQTQNQPEHGSTEQDYREERQEGLQARQQASERRRWRAPNWRPEPVVSVNILGINDFH
GQLSPRTVAGRPAGGAAVLASYLRSASREYTLIVHDGDQVGASPPNSALLRDEPAISLLNLLANPFCRPFSWQMKLPRSA
QPLAQQRCNVVGTLGNHEFDYGKNELLRQLTGGNHANGPYLEENWQGARYPTVSSNVINQSSGRSLLPPHTIYNAGGVRI
GVIGAALKETPSIVSPSGVAGLRFIDEAAAINRSVAQLRRQGVRAIIVALHQGGQQTSYNGPTNAEADTVVGPVVDIVKR
LDDEVDVVISGHAHGFTNALLPNANGKPILVTQAFSAGTAYADIELLVSRRSRDVVEKSAAILTTWADQGAGLTPDPQVA
ALVAQADARVEPLVARVVGLAQGAITRTETPAGESALGNLIADAQRAATGAQISFMNPGGIRADLDGGEVTWGELFAIQP
FANDLVSMDLTGAQIKTLLEQQWIGQSYPRLLKPSGILYSWAANRPEGNRVIEMRDASGAPLNPAATYRVTVNSFLAGGG
DNFTILNEGQNRVVGPVDLDALVGYIEALPQPFSASVEGRIQRVD

Specific function: Sugar-nonspecific endonuclease that yields nucleotide 3'-monophosphate products. No 5'-nucleotidase activity was detected, using 5'-AMP as the substrate, in the presence of diverse divalent metals and with various pH values [H]

COG id: COG0737

COG function: function code F; 5'-nucleotidase/2',3'-cyclic phosphodiesterase and related esterases

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TNase-like domain [H]

Homologues:

Organism=Homo sapiens, GI4505467, Length=460, Percent_Identity=25.2173913043478, Blast_Score=104, Evalue=2e-22,
Organism=Escherichia coli, GI1786687, Length=574, Percent_Identity=23.3449477351916, Blast_Score=107, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24641187, Length=492, Percent_Identity=27.6422764227642, Blast_Score=125, Evalue=1e-28,
Organism=Drosophila melanogaster, GI221329836, Length=492, Percent_Identity=26.4227642276423, Blast_Score=116, Evalue=4e-26,
Organism=Drosophila melanogaster, GI19922444, Length=474, Percent_Identity=24.4725738396624, Blast_Score=101, Evalue=2e-21,
Organism=Drosophila melanogaster, GI19922446, Length=464, Percent_Identity=25.2155172413793, Blast_Score=96, Evalue=7e-20,
Organism=Drosophila melanogaster, GI24654424, Length=464, Percent_Identity=25.2155172413793, Blast_Score=96, Evalue=7e-20,
Organism=Drosophila melanogaster, GI28573524, Length=462, Percent_Identity=23.8095238095238, Blast_Score=92, Evalue=1e-18,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008334
- InterPro:   IPR006146
- InterPro:   IPR006179
- InterPro:   IPR019948
- InterPro:   IPR019931
- InterPro:   IPR004843
- InterPro:   IPR016027
- InterPro:   IPR004365
- InterPro:   IPR016071
- InterPro:   IPR006021 [H]

Pfam domain/function: PF02872 5_nucleotid_C; PF00746 Gram_pos_anchor; PF00149 Metallophos; PF00565 SNase; PF01336 tRNA_anti [H]

EC number: 3.6.1.45; 3.1.3.5 [C]

Molecular weight: Translated: 64733; Mature: 64733

Theoretical pI: Translated: 6.26; Mature: 6.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRMRVLDWLFAAGLIGLAAPTLAQTQNQPEHGSTEQDYREERQEGLQARQQASERRRWRA
CCHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
PNWRPEPVVSVNILGINDFHGQLSPRTVAGRPAGGAAVLASYLRSASREYTLIVHDGDQV
CCCCCCCEEEEEEEEEECCCCCCCCCEECCCCCCHHHHHHHHHHHCCCCEEEEEECCCCC
GASPPNSALLRDEPAISLLNLLANPFCRPFSWQMKLPRSAQPLAQQRCNVVGTLGNHEFD
CCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCEEECCCCCCCHHHHHHCCEEECCCCCCCC
YGKNELLRQLTGGNHANGPYLEENWQGARYPTVSSNVINQSSGRSLLPPHTIYNAGGVRI
CCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCHHEECCCCEEE
GVIGAALKETPSIVSPSGVAGLRFIDEAAAINRSVAQLRRQGVRAIIVALHQGGQQTSYN
EEEHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHEEEHHHHCCCCCCCCC
GPTNAEADTVVGPVVDIVKRLDDEVDVVISGHAHGFTNALLPNANGKPILVTQAFSAGTA
CCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHCCCCCCCCEEEEEECCCCCCH
YADIELLVSRRSRDVVEKSAAILTTWADQGAGLTPDPQVAALVAQADARVEPLVARVVGL
HHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHH
AQGAITRTETPAGESALGNLIADAQRAATGAQISFMNPGGIRADLDGGEVTWGELFAIQP
HHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCEEECCCCEEEHHHEEEECH
FANDLVSMDLTGAQIKTLLEQQWIGQSYPRLLKPSGILYSWAANRPEGNRVIEMRDASGA
HHHHHHCCCCCHHHHHHHHHHHHCCCCCCHHCCCCCEEEEECCCCCCCCEEEEEECCCCC
PLNPAATYRVTVNSFLAGGGDNFTILNEGQNRVVGPVDLDALVGYIEALPQPFSASVEGR
CCCCCEEEEEEEEHHHCCCCCCEEEEECCCCCEECCCCHHHHHHHHHHCCCCCCCCCCCE
IQRVD
EEECC
>Mature Secondary Structure
MRMRVLDWLFAAGLIGLAAPTLAQTQNQPEHGSTEQDYREERQEGLQARQQASERRRWRA
CCHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
PNWRPEPVVSVNILGINDFHGQLSPRTVAGRPAGGAAVLASYLRSASREYTLIVHDGDQV
CCCCCCCEEEEEEEEEECCCCCCCCCEECCCCCCHHHHHHHHHHHCCCCEEEEEECCCCC
GASPPNSALLRDEPAISLLNLLANPFCRPFSWQMKLPRSAQPLAQQRCNVVGTLGNHEFD
CCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCEEECCCCCCCHHHHHHCCEEECCCCCCCC
YGKNELLRQLTGGNHANGPYLEENWQGARYPTVSSNVINQSSGRSLLPPHTIYNAGGVRI
CCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCHHEECCCCEEE
GVIGAALKETPSIVSPSGVAGLRFIDEAAAINRSVAQLRRQGVRAIIVALHQGGQQTSYN
EEEHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHEEEHHHHCCCCCCCCC
GPTNAEADTVVGPVVDIVKRLDDEVDVVISGHAHGFTNALLPNANGKPILVTQAFSAGTA
CCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHCCCCCCCCEEEEEECCCCCCH
YADIELLVSRRSRDVVEKSAAILTTWADQGAGLTPDPQVAALVAQADARVEPLVARVVGL
HHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHH
AQGAITRTETPAGESALGNLIADAQRAATGAQISFMNPGGIRADLDGGEVTWGELFAIQP
HHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCEEECCCCEEEHHHEEEECH
FANDLVSMDLTGAQIKTLLEQQWIGQSYPRLLKPSGILYSWAANRPEGNRVIEMRDASGA
HHHHHHCCCCCHHHHHHHHHHHHCCCCCCHHCCCCCEEEEECCCCCCCCEEEEEECCCCC
PLNPAATYRVTVNSFLAGGGDNFTILNEGQNRVVGPVDLDALVGYIEALPQPFSASVEGR
CCCCCEEEEEEEEHHHCCCCCCEEEEECCCCCEECCCCHHHHHHHHHHCCCCCCCCCCCE
IQRVD
EEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: H2O; UMP [C]

Specific reaction: H2O + UMP --> Phosphate + Uridine [C]

General reaction: Phosphorous acid anhydride hydrolysis [C]

Inhibitor: Inhibitor from Salmonella typhimurium cytoplasm [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969498; 9384377 [H]