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The map label for this gene is algB [H]

Identifier: 146284365

GI number: 146284365

Start: 4406753

End: 4408099

Strand: Direct

Name: algB [H]

Synonym: PST_4053

Alternate gene names: 146284365

Gene position: 4406753-4408099 (Clockwise)

Preceding gene: 146284364

Following gene: 146284366

Centisome position: 96.48

GC content: 66.22

Gene sequence:

>1347_bases
ATGGACCAAACGACGGACAACGGCGGACGCATCCTCCTGGTGGATGACGAAGCGGCGATCCTGCGTACCTTTCGTTACTG
CCTGGAAGACCGTGGCTATACGGTGATGACGGCAGCCAGCGCTGCGCAGGCCGAAGCGATCCTGCAACGCCAGGTGTTCG
ATCTGTGCTTCCTTGACCTGCGCCTGGGCGAAGACAACGGTCTGGACGTGCTGCAGCAGATGCGCACGCTGGCGCCCTGG
ATGCGCGTGGTGATCGTCACCGCCCACTCGGCGGTGGACACAGCGGTCGATGCCATGCAGGCCGGCGCCGCGGATTACCT
GCTCAAGCCCTGCAGCCCCGAACAGCTGCGTCTGTCGGCCGCCAAGCAGCTGGAAGTCCGGCAGATGGCAGCGCGCCTGG
AAGCACTGGAAGGCGAGATGAAGCAGCGCAGCGATGCGCTTGGCTCGCACAGCCCGGCAATGATGGCCGTGCTGGAGACC
GCCCGGCAGGTCGCCGACACCGATGCCAACATCCTCATCCTCGGCGAATCCGGCACCGGCAAGGGCGAGCTGGCGCGTGC
CATCCACACCTGGAGCCGGCGCTCGAAGAAGGCCTTCGTCACCATCAACTGCCCGTCACTGTCGGCCGACCTGATGGAAA
GCGAGCTGTTCGGGCACAACCGCGGTGCCTTCACCGGAGCCACCGAAAGCACCCTCGGTCGGGTCAACCAGGCCGACGGC
GGCACGCTGTTCCTCGACGAGATCGGCGACTTCCCGCTGGCGCTGCAACCCAAGCTGCTGCGCTTCATTCAGGACAAGGA
ATACGAACGCGTCGGCGACCCGGTCACCCGCCGCGCCGATGTGCGCATCCTGGCGGCGACCAACCTCAATCTCGAGGAGA
TGGTCCGCGAGGGCAAGTTCCGCGAGGACCTGCTCTACCGCCTCAACGTCATCACCCTCAATCTGCCACCGATGCGCGAG
CGCCCGGAAGACGTACTGGCCCTGGCCGAGCGCTTCCTCGCCGCCTTCGTCAAGAACTACGGGCGCCCGGCGCGCGGCTT
CAGCGATGCTGCGGTGGCGGCGCTCAAGGCCTATCGCTGGCCCGGCAACGTGCGCGAACTGCGCAACGTGGTGGAGCGGG
CAAGCATCATCTGCCCGCAGCAGATGATCGAGGTCAACCACCTCGGGCTCGGCGAACAGGCAGGTAGCAACGCGCCGCGC
ATCGGCGAGCCGCTGAGCCTCGAGGCACTGGAAAAGGCGCACATCGCCGGCGTGCTGAGCACCAGCGACACACTGGAGCA
GGCCGCACGCATCCTCGGCATCGACGCCTCGACCCTGTACAGAAAGCGCAAGCAGTACGGCCTATGA

Upstream 100 bases:

>100_bases
CTAAAGCACTCGCCAGCAACGCAACGGCGCCGCGACGGCGCTCGACACGCCATAATCGAATCTCTACAGCGACCGAACTC
GCCTTTTGATGGGAGCATCC

Downstream 100 bases:

>100_bases
AACTGCAGATGAAGCTGCGAACCCGACTCTTCCTCGGGTTCTCGGCACTGATGACGGTGGCCCTGCTGGGCCTGCTGCTG
GCGCTGGTCAGCGTGATGCA

Product: alginate biosynthesis transcriptional regulatory protein AlgB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 448; Mature: 448

Protein sequence:

>448_residues
MDQTTDNGGRILLVDDEAAILRTFRYCLEDRGYTVMTAASAAQAEAILQRQVFDLCFLDLRLGEDNGLDVLQQMRTLAPW
MRVVIVTAHSAVDTAVDAMQAGAADYLLKPCSPEQLRLSAAKQLEVRQMAARLEALEGEMKQRSDALGSHSPAMMAVLET
ARQVADTDANILILGESGTGKGELARAIHTWSRRSKKAFVTINCPSLSADLMESELFGHNRGAFTGATESTLGRVNQADG
GTLFLDEIGDFPLALQPKLLRFIQDKEYERVGDPVTRRADVRILAATNLNLEEMVREGKFREDLLYRLNVITLNLPPMRE
RPEDVLALAERFLAAFVKNYGRPARGFSDAAVAALKAYRWPGNVRELRNVVERASIICPQQMIEVNHLGLGEQAGSNAPR
IGEPLSLEALEKAHIAGVLSTSDTLEQAARILGIDASTLYRKRKQYGL

Sequences:

>Translated_448_residues
MDQTTDNGGRILLVDDEAAILRTFRYCLEDRGYTVMTAASAAQAEAILQRQVFDLCFLDLRLGEDNGLDVLQQMRTLAPW
MRVVIVTAHSAVDTAVDAMQAGAADYLLKPCSPEQLRLSAAKQLEVRQMAARLEALEGEMKQRSDALGSHSPAMMAVLET
ARQVADTDANILILGESGTGKGELARAIHTWSRRSKKAFVTINCPSLSADLMESELFGHNRGAFTGATESTLGRVNQADG
GTLFLDEIGDFPLALQPKLLRFIQDKEYERVGDPVTRRADVRILAATNLNLEEMVREGKFREDLLYRLNVITLNLPPMRE
RPEDVLALAERFLAAFVKNYGRPARGFSDAAVAALKAYRWPGNVRELRNVVERASIICPQQMIEVNHLGLGEQAGSNAPR
IGEPLSLEALEKAHIAGVLSTSDTLEQAARILGIDASTLYRKRKQYGL
>Mature_448_residues
MDQTTDNGGRILLVDDEAAILRTFRYCLEDRGYTVMTAASAAQAEAILQRQVFDLCFLDLRLGEDNGLDVLQQMRTLAPW
MRVVIVTAHSAVDTAVDAMQAGAADYLLKPCSPEQLRLSAAKQLEVRQMAARLEALEGEMKQRSDALGSHSPAMMAVLET
ARQVADTDANILILGESGTGKGELARAIHTWSRRSKKAFVTINCPSLSADLMESELFGHNRGAFTGATESTLGRVNQADG
GTLFLDEIGDFPLALQPKLLRFIQDKEYERVGDPVTRRADVRILAATNLNLEEMVREGKFREDLLYRLNVITLNLPPMRE
RPEDVLALAERFLAAFVKNYGRPARGFSDAAVAALKAYRWPGNVRELRNVVERASIICPQQMIEVNHLGLGEQAGSNAPR
IGEPLSLEALEKAHIAGVLSTSDTLEQAARILGIDASTLYRKRKQYGL

Specific function: Positive regulator of the alginate biosynthetic gene algD [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=461, Percent_Identity=38.8286334056399, Blast_Score=302, Evalue=3e-83,
Organism=Escherichia coli, GI1790437, Length=445, Percent_Identity=40.8988764044944, Blast_Score=301, Evalue=4e-83,
Organism=Escherichia coli, GI1790299, Length=470, Percent_Identity=38.2978723404255, Blast_Score=273, Evalue=2e-74,
Organism=Escherichia coli, GI1788905, Length=439, Percent_Identity=38.2687927107062, Blast_Score=268, Evalue=8e-73,
Organism=Escherichia coli, GI87082117, Length=260, Percent_Identity=48.8461538461538, Blast_Score=242, Evalue=3e-65,
Organism=Escherichia coli, GI1789087, Length=305, Percent_Identity=42.6229508196721, Blast_Score=236, Evalue=2e-63,
Organism=Escherichia coli, GI1789233, Length=302, Percent_Identity=42.7152317880795, Blast_Score=232, Evalue=3e-62,
Organism=Escherichia coli, GI87081872, Length=236, Percent_Identity=45.3389830508475, Blast_Score=204, Evalue=7e-54,
Organism=Escherichia coli, GI87082152, Length=312, Percent_Identity=40.3846153846154, Blast_Score=204, Evalue=1e-53,
Organism=Escherichia coli, GI1787583, Length=304, Percent_Identity=37.5, Blast_Score=201, Evalue=1e-52,
Organism=Escherichia coli, GI1786524, Length=322, Percent_Identity=41.6149068322981, Blast_Score=199, Evalue=2e-52,
Organism=Escherichia coli, GI1789828, Length=254, Percent_Identity=35.4330708661417, Blast_Score=130, Evalue=1e-31,
Organism=Escherichia coli, GI87081858, Length=329, Percent_Identity=29.483282674772, Blast_Score=130, Evalue=1e-31,
Organism=Escherichia coli, GI1789809, Length=107, Percent_Identity=31.7757009345794, Blast_Score=66, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 49336; Mature: 49336

Theoretical pI: Translated: 5.57; Mature: 5.57

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDQTTDNGGRILLVDDEAAILRTFRYCLEDRGYTVMTAASAAQAEAILQRQVFDLCFLDL
CCCCCCCCCEEEEECCCHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHHHHHHHHHHE
RLGEDNGLDVLQQMRTLAPWMRVVIVTAHSAVDTAVDAMQAGAADYLLKPCSPEQLRLSA
EECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCHHHHHHHH
AKQLEVRQMAARLEALEGEMKQRSDALGSHSPAMMAVLETARQVADTDANILILGESGTG
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCC
KGELARAIHTWSRRSKKAFVTINCPSLSADLMESELFGHNRGAFTGATESTLGRVNQADG
HHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHCCCCCCCCCCCHHHHHCCCCCCC
GTLFLDEIGDFPLALQPKLLRFIQDKEYERVGDPVTRRADVRILAATNLNLEEMVREGKF
CEEEHHCCCCCCCCCCHHHHHHHHCCHHHHHCCCCCCCCCEEEEEECCCCHHHHHHCCCH
REDLLYRLNVITLNLPPMRERPEDVLALAERFLAAFVKNYGRPARGFSDAAVAALKAYRW
HHHHHHEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCC
PGNVRELRNVVERASIICPQQMIEVNHLGLGEQAGSNAPRIGEPLSLEALEKAHIAGVLS
CCCHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHC
TSDTLEQAARILGIDASTLYRKRKQYGL
CHHHHHHHHHHHCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MDQTTDNGGRILLVDDEAAILRTFRYCLEDRGYTVMTAASAAQAEAILQRQVFDLCFLDL
CCCCCCCCCEEEEECCCHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHHHHHHHHHHE
RLGEDNGLDVLQQMRTLAPWMRVVIVTAHSAVDTAVDAMQAGAADYLLKPCSPEQLRLSA
EECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCHHHHHHHH
AKQLEVRQMAARLEALEGEMKQRSDALGSHSPAMMAVLETARQVADTDANILILGESGTG
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCC
KGELARAIHTWSRRSKKAFVTINCPSLSADLMESELFGHNRGAFTGATESTLGRVNQADG
HHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHCCCCCCCCCCCHHHHHCCCCCCC
GTLFLDEIGDFPLALQPKLLRFIQDKEYERVGDPVTRRADVRILAATNLNLEEMVREGKF
CEEEHHCCCCCCCCCCHHHHHHHHCCHHHHHCCCCCCCCCEEEEEECCCCHHHHHHCCCH
REDLLYRLNVITLNLPPMRERPEDVLALAERFLAAFVKNYGRPARGFSDAAVAALKAYRW
HHHHHHEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCC
PGNVRELRNVVERASIICPQQMIEVNHLGLGEQAGSNAPRIGEPLSLEALEKAHIAGVLS
CCCHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHC
TSDTLEQAARILGIDASTLYRKRKQYGL
CHHHHHHHHHHHCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12534463 [H]