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The map label for this gene is rlmI [H]

Identifier: 146284322

GI number: 146284322

Start: 4347849

End: 4349045

Strand: Direct

Name: rlmI [H]

Synonym: PST_4010

Alternate gene names: 146284322

Gene position: 4347849-4349045 (Clockwise)

Preceding gene: 146284321

Following gene: 146284324

Centisome position: 95.19

GC content: 65.33

Gene sequence:

>1197_bases
ATGACCCTGCCCAGCCTGCGTCTCAAAGCCAATGCCGACCGCCGCCTGCGCGCGGGCCATCTGTGGGTCTACAGTAACGA
AGTCGACACGGCCGCCACGCCGCTGGCCGGCTTCGCCGCCGGCGACCAGGCCATCCTCGAAGCCGCCGGTGGCAAACCGC
TGGGTATCGTCGGGGTTTCGCCGAACAACCTGATCTGCGCGCGGCTGCTGTCGCGCGATCTCAAGCACAGCCTGGACAAG
TCGCTGCTGGTGCATCGCATCCAGGTCGCGCTGTCGCTGCGCGAGCGCCTGTTCGATCAGCCGTGCTACCGCCTGATCTA
CGGTGATTCCGACCTGCTGCCGGGCCTGGTGGTGGACCGCTTCTACGACCACCTGGTGGTGCAGCTGGCTTCGGCCACCA
TGGAGCGCAACAAGGATGCCGTGCTGGAAGCGCTGGTGCAGGTGCTCAAGCCGCGCGGCGTGCTGTGGAAGAACGACTCC
AGCGCCCGCGATGCCGAAGGCCTTGAACGCTACGTCGATACCGCCTTCGGCGTGGTACCGGAGTGGGTCGCGCTGGAAGA
GAACGGCGTGAAGTTCGAGGCGCCGGTGCTGCAGGGCCAGAAGACCGGCTGGTTCTACGATCACCGTATGAACCGCGCGC
GCCTGGCGCCCTATGTCAAGGGCAAGCGCGTGCTCGACCTGTTCAGCTACATCGGTGGCTGGGGCGTGCAGGCCGCCGCC
TTCGGCGCCAGCGAAGTGTTCTGTGTCGACGCCTCGGCCTTTGCCCTGGATGGCGTGGAGCGCAACGCTGCGCTCAATGG
TGTGGCCGAGAAGATGACCTGCGTCGAAGGCGATGCCTTCGAGGCGATGAAGGAACTGAAGAATGCCGAAGAGCGCTTCG
ACGTGGTGATCACCGATCCGCCGGCGTTCATCAAGCGCAAGAAGGATCTGAAGAACGGCGAGGCCGCCTACCGCCGCCTC
AACGAAACCGCCATGCGGCTGCTGAACAAGGACGGTATCCTGGTCAGCGCCAGCTGCTCCATGCACCTGGAAGAAGACCA
CCTGCAGAACATCCTGCTCGGCAGCGCGCGTCATCTGGATCGCAACATCCAGCTGCTCGAGCGCGGCGCCCAGGGTCCGG
ATCACCCGGTGCATCCGGCGATCCCGGAGACCCGCTACATCAAGAGCCTGACCTGCCGCATCCTGCCCAACAGCTGA

Upstream 100 bases:

>100_bases
GAGGCATCGACAGGCACGCGGCGGGCGTTTTTCCTACGACCTCAGCGCATGCGGCGGGCCGCTCGTTTAGAATGCCCGTC
TTTTTTCCGGAGCCCTGCCG

Downstream 100 bases:

>100_bases
TCGGCCATCCGGTTGCGCAGGGCGGCGAACCGCCCTGCGCTTCCCCTCCCTTACTCGATACCCTTGCGCTCCACACTCCA
CAGCAGGCTGGTACAGAGCC

Product: hypothetical protein

Products: NA

Alternate protein names: 23S rRNA m5C1962 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase RlmI [H]

Number of amino acids: Translated: 398; Mature: 397

Protein sequence:

>398_residues
MTLPSLRLKANADRRLRAGHLWVYSNEVDTAATPLAGFAAGDQAILEAAGGKPLGIVGVSPNNLICARLLSRDLKHSLDK
SLLVHRIQVALSLRERLFDQPCYRLIYGDSDLLPGLVVDRFYDHLVVQLASATMERNKDAVLEALVQVLKPRGVLWKNDS
SARDAEGLERYVDTAFGVVPEWVALEENGVKFEAPVLQGQKTGWFYDHRMNRARLAPYVKGKRVLDLFSYIGGWGVQAAA
FGASEVFCVDASAFALDGVERNAALNGVAEKMTCVEGDAFEAMKELKNAEERFDVVITDPPAFIKRKKDLKNGEAAYRRL
NETAMRLLNKDGILVSASCSMHLEEDHLQNILLGSARHLDRNIQLLERGAQGPDHPVHPAIPETRYIKSLTCRILPNS

Sequences:

>Translated_398_residues
MTLPSLRLKANADRRLRAGHLWVYSNEVDTAATPLAGFAAGDQAILEAAGGKPLGIVGVSPNNLICARLLSRDLKHSLDK
SLLVHRIQVALSLRERLFDQPCYRLIYGDSDLLPGLVVDRFYDHLVVQLASATMERNKDAVLEALVQVLKPRGVLWKNDS
SARDAEGLERYVDTAFGVVPEWVALEENGVKFEAPVLQGQKTGWFYDHRMNRARLAPYVKGKRVLDLFSYIGGWGVQAAA
FGASEVFCVDASAFALDGVERNAALNGVAEKMTCVEGDAFEAMKELKNAEERFDVVITDPPAFIKRKKDLKNGEAAYRRL
NETAMRLLNKDGILVSASCSMHLEEDHLQNILLGSARHLDRNIQLLERGAQGPDHPVHPAIPETRYIKSLTCRILPNS
>Mature_397_residues
TLPSLRLKANADRRLRAGHLWVYSNEVDTAATPLAGFAAGDQAILEAAGGKPLGIVGVSPNNLICARLLSRDLKHSLDKS
LLVHRIQVALSLRERLFDQPCYRLIYGDSDLLPGLVVDRFYDHLVVQLASATMERNKDAVLEALVQVLKPRGVLWKNDSS
ARDAEGLERYVDTAFGVVPEWVALEENGVKFEAPVLQGQKTGWFYDHRMNRARLAPYVKGKRVLDLFSYIGGWGVQAAAF
GASEVFCVDASAFALDGVERNAALNGVAEKMTCVEGDAFEAMKELKNAEERFDVVITDPPAFIKRKKDLKNGEAAYRRLN
ETAMRLLNKDGILVSASCSMHLEEDHLQNILLGSARHLDRNIQLLERGAQGPDHPVHPAIPETRYIKSLTCRILPNS

Specific function: Specifically methylates the cytosine at position 1962 (m5C1962) of 23S rRNA [H]

COG id: COG1092

COG function: function code R; Predicted SAM-dependent methyltransferases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Escherichia coli, GI145693111, Length=397, Percent_Identity=35.264483627204, Blast_Score=217, Evalue=8e-58,
Organism=Escherichia coli, GI1787181, Length=247, Percent_Identity=29.5546558704453, Blast_Score=79, Evalue=6e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002478
- InterPro:   IPR015947
- InterPro:   IPR019614 [H]

Pfam domain/function: PF10672 Methyltrans_SAM [H]

EC number: =2.1.1.191 [H]

Molecular weight: Translated: 44050; Mature: 43919

Theoretical pI: Translated: 7.29; Mature: 7.29

Prosite motif: PS50890 PUA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLPSLRLKANADRRLRAGHLWVYSNEVDTAATPLAGFAAGDQAILEAAGGKPLGIVGVS
CCCCCCEEECCCCCCEECCEEEEECCCCCCHHCCCHHHCCCCHHHHHHCCCCCEEEEECC
PNNLICARLLSRDLKHSLDKSLLVHRIQVALSLRERLFDQPCYRLIYGDSDLLPGLVVDR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEEEECCCCCCCHHHHHH
FYDHLVVQLASATMERNKDAVLEALVQVLKPRGVLWKNDSSARDAEGLERYVDTAFGVVP
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCEECCCCCCCHHHHHHHHHHHHHCCCC
EWVALEENGVKFEAPVLQGQKTGWFYDHRMNRARLAPYVKGKRVLDLFSYIGGWGVQAAA
CCEEECCCCCEEECCCCCCCCCCEEHHHCCCHHHCCHHHCCHHHHHHHHHHCCCCCHHHH
FGASEVFCVDASAFALDGVERNAALNGVAEKMTCVEGDAFEAMKELKNAEERFDVVITDP
CCCCEEEEECCHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHCHHHHCCEEECCC
PAFIKRKKDLKNGEAAYRRLNETAMRLLNKDGILVSASCSMHLEEDHLQNILLGSARHLD
HHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHH
RNIQLLERGAQGPDHPVHPAIPETRYIKSLTCRILPNS
HHHHHHHCCCCCCCCCCCCCCCCHHHHHHHEEEECCCC
>Mature Secondary Structure 
TLPSLRLKANADRRLRAGHLWVYSNEVDTAATPLAGFAAGDQAILEAAGGKPLGIVGVS
CCCCCEEECCCCCCEECCEEEEECCCCCCHHCCCHHHCCCCHHHHHHCCCCCEEEEECC
PNNLICARLLSRDLKHSLDKSLLVHRIQVALSLRERLFDQPCYRLIYGDSDLLPGLVVDR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEEEECCCCCCCHHHHHH
FYDHLVVQLASATMERNKDAVLEALVQVLKPRGVLWKNDSSARDAEGLERYVDTAFGVVP
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCEECCCCCCCHHHHHHHHHHHHHCCCC
EWVALEENGVKFEAPVLQGQKTGWFYDHRMNRARLAPYVKGKRVLDLFSYIGGWGVQAAA
CCEEECCCCCEEECCCCCCCCCCEEHHHCCCHHHCCHHHCCHHHHHHHHHHCCCCCHHHH
FGASEVFCVDASAFALDGVERNAALNGVAEKMTCVEGDAFEAMKELKNAEERFDVVITDP
CCCCEEEEECCHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHCHHHHCCEEECCC
PAFIKRKKDLKNGEAAYRRLNETAMRLLNKDGILVSASCSMHLEEDHLQNILLGSARHLD
HHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHH
RNIQLLERGAQGPDHPVHPAIPETRYIKSLTCRILPNS
HHHHHHHCCCCCCCCCCCCCCCCHHHHHHHEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA