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The map label for this gene is rsmE [H]

Identifier: 146284257

GI number: 146284257

Start: 4277880

End: 4278596

Strand: Direct

Name: rsmE [H]

Synonym: PST_3945

Alternate gene names: 146284257

Gene position: 4277880-4278596 (Clockwise)

Preceding gene: 146284256

Following gene: 146284266

Centisome position: 93.66

GC content: 67.22

Gene sequence:

>717_bases
ATGCGCCTATCCCGCTTCTTCATCGACGCCCCTCTTTCCCTCGGCCAGCATGCGTTACCCGAGGCATCGGCTCATTACAT
CGGGCGAGTCCTGCGCCTGTCGGCCGGCAGCGCGGTGCAACTGTTCGACGGCAGCGGTGCCGAGTATCGCGGCGAACTGC
TGGAGGTGGGCAAGAAGAGCGTCAGCGTGGAACTGTACGAACGCCTGGCGGGAATGCCGGAATCACCGCTGCGAATCCAC
CTCGGTCAGGGCCTGTCCCGTGGCGAACGCATGGACTGGGCGATCCAGAAGGCCACCGAGCTGGGCGTCAGCGAAATCAC
CCCGCTGATCAGCGAGCGATGCGAGGTCCGCCTCAACGATGAGCGCGCCGACAAGCGCCTGGCCCATTGGCGACAGATCG
CCATCAGCGCCTGCGAGCAATGCGGTCGCTCGGTACTGCCGATCATCCATGCGCCGGTCACGCTGCGCGACTGGCTGGAC
GTCGAGGCCGACCTCAAGCTCGTGCTGCACCCGGTGGCCGAGCCGCTGGCGAGCCATGCCAAGCCGCAGAGCCTGGCATT
TCTGATCGGCCCGGAGGGCGGCCTGAGCGATAGCGAAGTCGAACTGGCCAGCGGCCAGGGGTTTCTGCCGGCCCGGCTCG
GCCCGCGCGTACTGCGCACCGAGACCGCACCGGTGGTGGCATTGAGCGTGGCGCAGCAGCTGTGGGGCGACTTCTAG

Upstream 100 bases:

>100_bases
GCGATCCGGGCTGAGCACCACGCCGCGTGCGGCGGCCTGCTGAGCGGGCTGCCGATGCTTTAGACTTGCCTCTCCATCCA
CCACGCCCTGCCGTTGCCCT

Downstream 100 bases:

>100_bases
CCGCGGACCGCAGTCAGATCAGGCCGGCGTCCGCCAGCTTCTGCTCCAGGCCGACCAGGTCGGGAATACGTGCCTGCATA
TCACCGAGGTTGACCGCATC

Product: 16S ribosomal RNA methyltransferase RsmE

Products: NA

Alternate protein names: 16S rRNA m3U1498 methyltransferase [H]

Number of amino acids: Translated: 238; Mature: 238

Protein sequence:

>238_residues
MRLSRFFIDAPLSLGQHALPEASAHYIGRVLRLSAGSAVQLFDGSGAEYRGELLEVGKKSVSVELYERLAGMPESPLRIH
LGQGLSRGERMDWAIQKATELGVSEITPLISERCEVRLNDERADKRLAHWRQIAISACEQCGRSVLPIIHAPVTLRDWLD
VEADLKLVLHPVAEPLASHAKPQSLAFLIGPEGGLSDSEVELASGQGFLPARLGPRVLRTETAPVVALSVAQQLWGDF

Sequences:

>Translated_238_residues
MRLSRFFIDAPLSLGQHALPEASAHYIGRVLRLSAGSAVQLFDGSGAEYRGELLEVGKKSVSVELYERLAGMPESPLRIH
LGQGLSRGERMDWAIQKATELGVSEITPLISERCEVRLNDERADKRLAHWRQIAISACEQCGRSVLPIIHAPVTLRDWLD
VEADLKLVLHPVAEPLASHAKPQSLAFLIGPEGGLSDSEVELASGQGFLPARLGPRVLRTETAPVVALSVAQQLWGDF
>Mature_238_residues
MRLSRFFIDAPLSLGQHALPEASAHYIGRVLRLSAGSAVQLFDGSGAEYRGELLEVGKKSVSVELYERLAGMPESPLRIH
LGQGLSRGERMDWAIQKATELGVSEITPLISERCEVRLNDERADKRLAHWRQIAISACEQCGRSVLPIIHAPVTLRDWLD
VEADLKLVLHPVAEPLASHAKPQSLAFLIGPEGGLSDSEVELASGQGFLPARLGPRVLRTETAPVVALSVAQQLWGDF

Specific function: Specifically methylates the uridine in position 1498 of 16S rRNA in the fully assembled 30S ribosomal subunit [H]

COG id: COG1385

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase RsmE family [H]

Homologues:

Organism=Escherichia coli, GI87082186, Length=242, Percent_Identity=47.5206611570248, Blast_Score=204, Evalue=4e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015947
- InterPro:   IPR006700 [H]

Pfam domain/function: PF04452 Methyltrans_RNA [H]

EC number: =2.1.1.193 [H]

Molecular weight: Translated: 25966; Mature: 25966

Theoretical pI: Translated: 6.16; Mature: 6.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLSRFFIDAPLSLGQHALPEASAHYIGRVLRLSAGSAVQLFDGSGAEYRGELLEVGKKS
CCCCCEEECCCHHCCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHCHHH
VSVELYERLAGMPESPLRIHLGQGLSRGERMDWAIQKATELGVSEITPLISERCEVRLND
HHHHHHHHHCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHCEEECH
ERADKRLAHWRQIAISACEQCGRSVLPIIHAPVTLRDWLDVEADLKLVLHPVAEPLASHA
HHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCEEHHHHHCCCHHHHHHHHHHHHHHHHCC
KPQSLAFLIGPEGGLSDSEVELASGQGFLPARLGPRVLRTETAPVVALSVAQQLWGDF
CCCCEEEEECCCCCCCCCCEEECCCCCCCCHHCCCHHHHCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRLSRFFIDAPLSLGQHALPEASAHYIGRVLRLSAGSAVQLFDGSGAEYRGELLEVGKKS
CCCCCEEECCCHHCCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHCHHH
VSVELYERLAGMPESPLRIHLGQGLSRGERMDWAIQKATELGVSEITPLISERCEVRLND
HHHHHHHHHCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHCEEECH
ERADKRLAHWRQIAISACEQCGRSVLPIIHAPVTLRDWLDVEADLKLVLHPVAEPLASHA
HHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCEEHHHHHCCCHHHHHHHHHHHHHHHHCC
KPQSLAFLIGPEGGLSDSEVELASGQGFLPARLGPRVLRTETAPVVALSVAQQLWGDF
CCCCEEEEECCCCCCCCCCEEECCCCCCCCHHCCCHHHHCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8332061 [H]