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The map label for this gene is metK

Identifier: 146284238

GI number: 146284238

Start: 4256153

End: 4257343

Strand: Direct

Name: metK

Synonym: PST_3926

Alternate gene names: 146284238

Gene position: 4256153-4257343 (Clockwise)

Preceding gene: 146284237

Following gene: 146284239

Centisome position: 93.19

GC content: 66.08

Gene sequence:

>1191_bases
ATGAGCGAATACTCGATTTTCACCTCCGAGTCCGTATCCGAAGGGCATCCGGACAAGATCGCCGACCAGATCTCCGATGC
GGTGCTCGACGCCATCATCGCCGAGGACAAGCACGCCCGCGTGGCCTGCGAAACCCTGGTCAAGACCGGTGTGGCCATCG
TCGCCGGTGAAGTGACCACCAGCGCCTGGGTCGATCTGGAGCAACTGGTGCGCGACGTCATCGTCGACATCGGCTACAAC
AGCTCGGAAGTCGGTTTCGACGGCGCCACCTGCGGCATCATCAACATCATCGGCAAGCAGTCGGTGGACATCGCCCAGGG
CGTCGACCGCACCAAGCCGGAGGACCAGGGTGCCGGCGACCAGGGCCTGATGTTCGGCTATGCCAGCAACGAGACCGACG
TACTGATGCCGGCGCCGATCCGTTTCTCCCACGCCCTGGTCGAGCGCCAGGCCGAGGCGCGCAAGAACGGCCTGCTGCCG
TGGCTGCGTCCGGACGCCAAGAGCCAGGTCACCTGCCGCTACGAGAACGGCCAGGTGGTCGGCATCGACGCCGTGGTGCT
GTCGACCCAGCACAACCCCGACGTCAAGCAGAGCGATCTGCGCGAAGCGGTGATGGAGCTGATCATCAAGCACAGCCTGC
CGGCCGAGCTGCTGCACAAGGACACCCAGTACCACATCAACCCGACCGGCCAGTTCGTCATCGGCGGCCCGGTAGGCGAC
TGCGGCCTGACCGGACGCAAGATCATCGTCGACACCTACGGCGGCATGGCCCGTCACGGCGGCGGCGCCTTCTCCGGCAA
GGACCCGTCCAAGGTCGACCGCAGCGCTGCCTACGCCGGACGCTACGTCGCCAAGAACATCGTTGCCGCCGGTCTCGCCG
ATCGCTGCGAGATCCAGGTGTCCTATGCCATCGGCGTGGCGCAGCCGACGTCCATCTCGCTGAACACCTTCGGCACCGGC
AAGCTCGGCGATGACAAGATCATCGCCCTGGTGCGCGAGCACTTCGATCTGCGCCCCTACGCGATCACCCGCATGCTCGA
CCTGCTGCACCCGATGTATCGCGCCACCGCCGCCTACGGCCACTTCGGCCGCACCCCCTATGAGATGACGGTGGGTGCCG
ACACCTTCACCGCCTTCACCTGGGAGAAGACCGACAAGGCCGACGCCCTGCGCGCCGCCGCCGGGCTGTAA

Upstream 100 bases:

>100_bases
TCTACATTGGCTTGAAAAACGGTTTTCAGATCCAGGCCCGGTGCTTTTCCCGGTCCGCTCCTGACGACCGACTCACCCAC
CGGTAATAGGAAACCGATAG

Downstream 100 bases:

>100_bases
GCAGCCTCCGGTCAACGGAAAAACCCCGCCGGCCTGAGCCGTGCGGGGTTTTCTGCTTTCTGGACCGGATACATCCAGCG
TCACGCAATCCGCACCAGGT

Product: S-adenosylmethionine synthetase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase

Number of amino acids: Translated: 396; Mature: 395

Protein sequence:

>396_residues
MSEYSIFTSESVSEGHPDKIADQISDAVLDAIIAEDKHARVACETLVKTGVAIVAGEVTTSAWVDLEQLVRDVIVDIGYN
SSEVGFDGATCGIINIIGKQSVDIAQGVDRTKPEDQGAGDQGLMFGYASNETDVLMPAPIRFSHALVERQAEARKNGLLP
WLRPDAKSQVTCRYENGQVVGIDAVVLSTQHNPDVKQSDLREAVMELIIKHSLPAELLHKDTQYHINPTGQFVIGGPVGD
CGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAAYAGRYVAKNIVAAGLADRCEIQVSYAIGVAQPTSISLNTFGTG
KLGDDKIIALVREHFDLRPYAITRMLDLLHPMYRATAAYGHFGRTPYEMTVGADTFTAFTWEKTDKADALRAAAGL

Sequences:

>Translated_396_residues
MSEYSIFTSESVSEGHPDKIADQISDAVLDAIIAEDKHARVACETLVKTGVAIVAGEVTTSAWVDLEQLVRDVIVDIGYN
SSEVGFDGATCGIINIIGKQSVDIAQGVDRTKPEDQGAGDQGLMFGYASNETDVLMPAPIRFSHALVERQAEARKNGLLP
WLRPDAKSQVTCRYENGQVVGIDAVVLSTQHNPDVKQSDLREAVMELIIKHSLPAELLHKDTQYHINPTGQFVIGGPVGD
CGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAAYAGRYVAKNIVAAGLADRCEIQVSYAIGVAQPTSISLNTFGTG
KLGDDKIIALVREHFDLRPYAITRMLDLLHPMYRATAAYGHFGRTPYEMTVGADTFTAFTWEKTDKADALRAAAGL
>Mature_395_residues
SEYSIFTSESVSEGHPDKIADQISDAVLDAIIAEDKHARVACETLVKTGVAIVAGEVTTSAWVDLEQLVRDVIVDIGYNS
SEVGFDGATCGIINIIGKQSVDIAQGVDRTKPEDQGAGDQGLMFGYASNETDVLMPAPIRFSHALVERQAEARKNGLLPW
LRPDAKSQVTCRYENGQVVGIDAVVLSTQHNPDVKQSDLREAVMELIIKHSLPAELLHKDTQYHINPTGQFVIGGPVGDC
GLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAAYAGRYVAKNIVAAGLADRCEIQVSYAIGVAQPTSISLNTFGTGK
LGDDKIIALVREHFDLRPYAITRMLDLLHPMYRATAAYGHFGRTPYEMTVGADTFTAFTWEKTDKADALRAAAGL

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family

Homologues:

Organism=Homo sapiens, GI4557737, Length=372, Percent_Identity=56.7204301075269, Blast_Score=399, Evalue=1e-111,
Organism=Homo sapiens, GI5174529, Length=372, Percent_Identity=55.9139784946236, Blast_Score=390, Evalue=1e-108,
Organism=Escherichia coli, GI1789311, Length=391, Percent_Identity=71.0997442455243, Blast_Score=577, Evalue=1e-166,
Organism=Caenorhabditis elegans, GI17538498, Length=382, Percent_Identity=54.1884816753927, Blast_Score=394, Evalue=1e-110,
Organism=Caenorhabditis elegans, GI17538494, Length=376, Percent_Identity=55.0531914893617, Blast_Score=392, Evalue=1e-109,
Organism=Caenorhabditis elegans, GI25145633, Length=370, Percent_Identity=55.4054054054054, Blast_Score=390, Evalue=1e-109,
Organism=Caenorhabditis elegans, GI17551082, Length=375, Percent_Identity=53.6, Blast_Score=387, Evalue=1e-108,
Organism=Caenorhabditis elegans, GI32565905, Length=376, Percent_Identity=49.2021276595745, Blast_Score=330, Evalue=7e-91,
Organism=Caenorhabditis elegans, GI32565911, Length=335, Percent_Identity=51.3432835820895, Blast_Score=322, Evalue=2e-88,
Organism=Caenorhabditis elegans, GI25145635, Length=323, Percent_Identity=52.6315789473684, Blast_Score=317, Evalue=6e-87,
Organism=Caenorhabditis elegans, GI32565903, Length=298, Percent_Identity=52.0134228187919, Blast_Score=289, Evalue=2e-78,
Organism=Caenorhabditis elegans, GI32565909, Length=89, Percent_Identity=59.5505617977528, Blast_Score=108, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI32565907, Length=50, Percent_Identity=66, Blast_Score=69, Evalue=5e-12,
Organism=Saccharomyces cerevisiae, GI6320710, Length=390, Percent_Identity=52.5641025641026, Blast_Score=390, Evalue=1e-109,
Organism=Saccharomyces cerevisiae, GI6323209, Length=367, Percent_Identity=54.4959128065395, Blast_Score=382, Evalue=1e-107,
Organism=Drosophila melanogaster, GI45552159, Length=383, Percent_Identity=49.8694516971279, Blast_Score=360, Evalue=1e-100,
Organism=Drosophila melanogaster, GI24580529, Length=383, Percent_Identity=49.8694516971279, Blast_Score=360, Evalue=1e-100,
Organism=Drosophila melanogaster, GI24580537, Length=383, Percent_Identity=49.8694516971279, Blast_Score=360, Evalue=1e-100,
Organism=Drosophila melanogaster, GI24580533, Length=383, Percent_Identity=49.8694516971279, Blast_Score=360, Evalue=1e-100,
Organism=Drosophila melanogaster, GI24580535, Length=383, Percent_Identity=49.8694516971279, Blast_Score=360, Evalue=1e-100,
Organism=Drosophila melanogaster, GI24580531, Length=383, Percent_Identity=49.8694516971279, Blast_Score=360, Evalue=1e-100,
Organism=Drosophila melanogaster, GI24580527, Length=383, Percent_Identity=50.1305483028721, Blast_Score=357, Evalue=9e-99,
Organism=Drosophila melanogaster, GI24580525, Length=383, Percent_Identity=50.1305483028721, Blast_Score=357, Evalue=9e-99,
Organism=Drosophila melanogaster, GI24580541, Length=385, Percent_Identity=48.0519480519481, Blast_Score=335, Evalue=2e-92,
Organism=Drosophila melanogaster, GI24580539, Length=117, Percent_Identity=52.1367521367521, Blast_Score=122, Evalue=6e-28,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): METK_PSEU5 (A4VRE8)

Other databases:

- EMBL:   CP000304
- RefSeq:   YP_001174391.1
- ProteinModelPortal:   A4VRE8
- SMR:   A4VRE8
- STRING:   A4VRE8
- GeneID:   5096887
- GenomeReviews:   CP000304_GR
- KEGG:   psa:PST_3926
- eggNOG:   COG0192
- HOGENOM:   HBG443662
- OMA:   IYYAHRL
- PhylomeDB:   A4VRE8
- ProtClustDB:   PRK05250
- BioCyc:   PSTU379731:PST_3926-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00086
- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636
- PANTHER:   PTHR11964
- PIRSF:   PIRSF000497
- TIGRFAMs:   TIGR01034

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N; SSF55973 S-AdoMet_synt

EC number: =2.5.1.6

Molecular weight: Translated: 42612; Mature: 42480

Theoretical pI: Translated: 5.26; Mature: 5.26

Prosite motif: PS00376 ADOMET_SYNTHASE_1; PS00377 ADOMET_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEYSIFTSESVSEGHPDKIADQISDAVLDAIIAEDKHARVACETLVKTGVAIVAGEVTT
CCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCEEEEECCCHH
SAWVDLEQLVRDVIVDIGYNSSEVGFDGATCGIINIIGKQSVDIAQGVDRTKPEDQGAGD
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCCCCCHHHHCCCCCCCCCCCCCC
QGLMFGYASNETDVLMPAPIRFSHALVERQAEARKNGLLPWLRPDAKSQVTCRYENGQVV
CCEEEEECCCCCCEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCEEE
GIDAVVLSTQHNPDVKQSDLREAVMELIIKHSLPAELLHKDTQYHINPTGQFVIGGPVGD
EEEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCHHHHCCCCCEEECCCCCEEECCCCCC
CGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAAYAGRYVAKNIVAAGLADRCEIQV
CCCCCCEEEEECCCCCHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEE
SYAIGVAQPTSISLNTFGTGKLGDDKIIALVREHFDLRPYAITRMLDLLHPMYRATAAYG
EEEEECCCCCEEEEECCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHC
HFGRTPYEMTVGADTFTAFTWEKTDKADALRAAAGL
CCCCCCEEEEECCCEEEEEEECCCCHHHHHHHHHCC
>Mature Secondary Structure 
SEYSIFTSESVSEGHPDKIADQISDAVLDAIIAEDKHARVACETLVKTGVAIVAGEVTT
CCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCEEEEECCCHH
SAWVDLEQLVRDVIVDIGYNSSEVGFDGATCGIINIIGKQSVDIAQGVDRTKPEDQGAGD
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCCCCCHHHHCCCCCCCCCCCCCC
QGLMFGYASNETDVLMPAPIRFSHALVERQAEARKNGLLPWLRPDAKSQVTCRYENGQVV
CCEEEEECCCCCCEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCEEE
GIDAVVLSTQHNPDVKQSDLREAVMELIIKHSLPAELLHKDTQYHINPTGQFVIGGPVGD
EEEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCHHHHCCCCCEEECCCCCEEECCCCCC
CGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAAYAGRYVAKNIVAAGLADRCEIQV
CCCCCCEEEEECCCCCHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEE
SYAIGVAQPTSISLNTFGTGKLGDDKIIALVREHFDLRPYAITRMLDLLHPMYRATAAYG
EEEEECCCCCEEEEECCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHC
HFGRTPYEMTVGADTFTAFTWEKTDKADALRAAAGL
CCCCCCEEEEECCCEEEEEEECCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA