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The map label for this gene is holA [H]

Identifier: 146284082

GI number: 146284082

Start: 4079223

End: 4080254

Strand: Direct

Name: holA [H]

Synonym: PST_3770

Alternate gene names: 146284082

Gene position: 4079223-4080254 (Clockwise)

Preceding gene: 146284081

Following gene: 146284083

Centisome position: 89.31

GC content: 66.76

Gene sequence:

>1032_bases
ATGAAGCTCTCCCCCGCCCAACTCGGCAAGCACCTGCAAGGCAGTCTCGCGCCCGTTTACGTGGTCTGTGGCGACGAGCA
TCTGCTGTGCCAGGAAGCCTGCGATGCGATTCGCGCTGCTACTCGCGCGCAGGGGTTCAGTGAGCGCCAGGTCTTTCATG
TCGAGACCGGCTTCGACTGGGGCCAGCTCATCGAGGCTGGCGCCAGCCTGTCGCTGTTCGCGGAAAAACGCCTGATCGAG
TTGCGCATTCCCAACGGCAAGCCAGGGGACAAGGGCGCCGCGGCGCTACTGGACTATCTGGCGCGCCCGGCCGACGACAC
GGTGCTGTTGATCAGCCTGCCCAAGCTCGACGGCAGTACGCAGAAGACCAAGTGGGCCAAGGCGCTGATCGAAGGCAAGG
ACGTGCAGTTCCTGCAGATCTGGCCGGTGGATGCCGCGCAGCTACCGCAATGGATCCGCCAGCGATTGGCCCAGGTCGGC
CTGTCCGCTGACCAGGAAGCCGTCGAGCTGATCGCCGCGAGAGTCGAAGGCAATCTGCTCGCCGCCGCCCAGGAAATCGA
GAAGCTGAAACTGCTGGCCGACGCCGGCCAGATCAGCGCCGATACCGTGCAAGCCGCCGTCGCCGACAGCGCCCGCTACG
ACGTCTTCGGCCTCATCGACGCGGCGCTCCACGGGCAGGCCGCCCATGCGCTGCACATGCTCGAGGGCTTGCGCGGCGAG
GGCGTGGAGGCACCAGTGATCCTCTGGGCATTGGCGCGCGAGCTGCGCCTGTTGGCCAACATCTCCCAGCAGTATGGCCA
GGGCGTTCCGCTCGATCGCGCCTTCAGCCAGGCTCGCCCACCAGTCTGGGACAAACGACGCCCGTTGGTCTCCAAGGCAC
TGCAACGGCACGACGCCGCAGGCTGGAGCCGCTTGCTGATGGCTGCACAGCGCATCGACGAGCAGATCAAGGGCCAGGCC
GAAGGCGACCCCTGGATCGGGCTGGCCAATATCTGCCTGCAGCTGAGCGGCCGCCGGCTGGGCGCGGGCTGA

Upstream 100 bases:

>100_bases
GCCGCCCGCCGCGCCCAGGACGCGCAACCGCAGCAGTCGCCCATCCAGCTACCCGTCCGCTGAGCCTGCACGGGGGCCAT
AGCGCCCCCGTCTTCTGCCC

Downstream 100 bases:

>100_bases
CATCGACGCCTGCTGGACATTTTTTGTACAAGCCCTATGATGCCCTCGCCCGATGCCGGGCTACCTGAGGGATACATCAT
GAGCACACGCAAGAAGCGGC

Product: DNA polymerase III subunit delta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 343; Mature: 343

Protein sequence:

>343_residues
MKLSPAQLGKHLQGSLAPVYVVCGDEHLLCQEACDAIRAATRAQGFSERQVFHVETGFDWGQLIEAGASLSLFAEKRLIE
LRIPNGKPGDKGAAALLDYLARPADDTVLLISLPKLDGSTQKTKWAKALIEGKDVQFLQIWPVDAAQLPQWIRQRLAQVG
LSADQEAVELIAARVEGNLLAAAQEIEKLKLLADAGQISADTVQAAVADSARYDVFGLIDAALHGQAAHALHMLEGLRGE
GVEAPVILWALARELRLLANISQQYGQGVPLDRAFSQARPPVWDKRRPLVSKALQRHDAAGWSRLLMAAQRIDEQIKGQA
EGDPWIGLANICLQLSGRRLGAG

Sequences:

>Translated_343_residues
MKLSPAQLGKHLQGSLAPVYVVCGDEHLLCQEACDAIRAATRAQGFSERQVFHVETGFDWGQLIEAGASLSLFAEKRLIE
LRIPNGKPGDKGAAALLDYLARPADDTVLLISLPKLDGSTQKTKWAKALIEGKDVQFLQIWPVDAAQLPQWIRQRLAQVG
LSADQEAVELIAARVEGNLLAAAQEIEKLKLLADAGQISADTVQAAVADSARYDVFGLIDAALHGQAAHALHMLEGLRGE
GVEAPVILWALARELRLLANISQQYGQGVPLDRAFSQARPPVWDKRRPLVSKALQRHDAAGWSRLLMAAQRIDEQIKGQA
EGDPWIGLANICLQLSGRRLGAG
>Mature_343_residues
MKLSPAQLGKHLQGSLAPVYVVCGDEHLLCQEACDAIRAATRAQGFSERQVFHVETGFDWGQLIEAGASLSLFAEKRLIE
LRIPNGKPGDKGAAALLDYLARPADDTVLLISLPKLDGSTQKTKWAKALIEGKDVQFLQIWPVDAAQLPQWIRQRLAQVG
LSADQEAVELIAARVEGNLLAAAQEIEKLKLLADAGQISADTVQAAVADSARYDVFGLIDAALHGQAAHALHMLEGLRGE
GVEAPVILWALARELRLLANISQQYGQGVPLDRAFSQARPPVWDKRRPLVSKALQRHDAAGWSRLLMAAQRIDEQIKGQA
EGDPWIGLANICLQLSGRRLGAG

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The delta subunit seems to interact with the gamma subunit to transfer the b

COG id: COG1466

COG function: function code L; DNA polymerase III, delta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786859, Length=341, Percent_Identity=32.8445747800587, Blast_Score=155, Evalue=3e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008921
- InterPro:   IPR010372
- InterPro:   IPR005790 [H]

Pfam domain/function: PF06144 DNA_pol3_delta [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 37148; Mature: 37148

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLSPAQLGKHLQGSLAPVYVVCGDEHLLCQEACDAIRAATRAQGFSERQVFHVETGFDW
CCCCHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCH
GQLIEAGASLSLFAEKRLIELRIPNGKPGDKGAAALLDYLARPADDTVLLISLPKLDGST
HHHHHCCCCCEEHHHCCEEEEECCCCCCCCHHHHHHHHHHHCCCCCEEEEEEECCCCCCC
QKTKWAKALIEGKDVQFLQIWPVDAAQLPQWIRQRLAQVGLSADQEAVELIAARVEGNLL
HHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCHH
AAAQEIEKLKLLADAGQISADTVQAAVADSARYDVFGLIDAALHGQAAHALHMLEGLRGE
HHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCHHHHHHHHHHCCHHHHHHHHHHCCCCC
GVEAPVILWALARELRLLANISQQYGQGVPLDRAFSQARPPVWDKRRPLVSKALQRHDAA
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHCHH
GWSRLLMAAQRIDEQIKGQAEGDPWIGLANICLQLSGRRLGAG
HHHHHHHHHHHHHHHHCCCCCCCCCEEHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MKLSPAQLGKHLQGSLAPVYVVCGDEHLLCQEACDAIRAATRAQGFSERQVFHVETGFDW
CCCCHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCH
GQLIEAGASLSLFAEKRLIELRIPNGKPGDKGAAALLDYLARPADDTVLLISLPKLDGST
HHHHHCCCCCEEHHHCCEEEEECCCCCCCCHHHHHHHHHHHCCCCCEEEEEEECCCCCCC
QKTKWAKALIEGKDVQFLQIWPVDAAQLPQWIRQRLAQVGLSADQEAVELIAARVEGNLL
HHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCHH
AAAQEIEKLKLLADAGQISADTVQAAVADSARYDVFGLIDAALHGQAAHALHMLEGLRGE
HHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCHHHHHHHHHHCCHHHHHHHHHHCCCCC
GVEAPVILWALARELRLLANISQQYGQGVPLDRAFSQARPPVWDKRRPLVSKALQRHDAA
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHCHH
GWSRLLMAAQRIDEQIKGQAEGDPWIGLANICLQLSGRRLGAG
HHHHHHHHHHHHHHHHCCCCCCCCCEEHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8505303; 1400251; 8905232; 9278503; 3316191; 8505304; 1575709 [H]