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The map label for this gene is miaB [H]

Identifier: 146284073

GI number: 146284073

Start: 4069034

End: 4070254

Strand: Direct

Name: miaB [H]

Synonym: PST_3761

Alternate gene names: 146284073

Gene position: 4069034-4070254 (Clockwise)

Preceding gene: 146284072

Following gene: 146284074

Centisome position: 89.09

GC content: 63.8

Gene sequence:

>1221_bases
ATGGAGATCACCGAGAATCCGGCAGAAGCCGACGTGATCCTGCTCAATACCTGCTCGATCCGCGAAAAGGCCCAGGAAAA
GGTCTTTTCCCAGCTGGGCCGCTGGCGCGAACTGAAACAGGACAATCCGCAGCTGGTGATCGGCGTCGGTGGCTGCGTCG
CCAGCCAGGAAGGCGCGGCGATCCGCGACCGCGCGCCCTATGTCGATGTGGTGTTCGGCCCGCAGACACTGCACCGCCTG
CCGGAAATGATCGATGCCGCGCGCACCACCAGGACGCCGCAGGTGGATATTTCCTTCCCCGAGATCGAGAAGTTCGACCG
CCTGCCCGAGCCGCGCGTCGACGGCCCCAGCGCCTATGTCTCGGTCATGGAAGGCTGCAGCAAGTACTGCACCTTCTGCG
TGGTGCCCTACACCCGCGGCGAGGAAGTCAGCCGGCCGCTGGCCGATGTGCTGGCGGAGATCGTTCACCTGGCCGAGAAC
GGCGTTAAGGAAGTCACCCTGCTGGGGCAGAACGTCAACGGCTATCGCCACGACGGCCACGACTTCGCCGACCTGCTGCA
TGCAGTGGCAGCGATCGATGGCATCGGACGCATCCGCTACACCACCAGCCATCCGCTGGAATTCTCCGACGCCATCATCC
AGGCCCACGCGGACATCCCGCAGCTGGTGAAATACCTGCACCTGCCGGTGCAGGCCGGTTCCGACCGCATCCTCGCAGCG
ATGAAGCGCAACCACACCGCGCTGGAGTACAAGTCGCGCATCCGCCGCCTGAAGGCCGCGGTCCCGGACATCCTGATCAG
CTCGGACTTCATCGTCGGCTTCCCCGGCGAGACGGACAAGGACTTTGAACAGACCATGAAGCTGATCGAGGACGTCGGCT
TCGACTTCTCCTACTCCTTCGTCTACAGCGCTCGTCCCGGCACCCCGGCGGCGGACCTCGCCGACGACACACCGGAGGAG
GTCAAGAAACAGCGCCTGGCGATCCTTCAGCAGCGCATCAACCAGCAGGGCTTCGAGAACAGCCGACGAATGGTCGGCAC
CACGCAGCGCATCCTGGTCAGCGACTACTCGAAAAAGGACCCCGGCATGCTGCAGGGCCGTACCGAGCACAATCGCATCG
TCAACTTCCGCTGCGACAACCCGCGACTGATCGGCCAGTTCGTCGACGTTCACATCGACGATGCGCTGCCACACTCGCTG
CGTGGCAGCCTGCTATCTTGA

Upstream 100 bases:

>100_bases
CCGTAACATGACCCGCAAGCTTTTCATCGAAACCCATGGCTGCCAGATGAACGAGTACGACAGCTCGCGCATGGTGGACC
TGCTGGGCGAACACCAGGCC

Downstream 100 bases:

>100_bases
GAGTGACGGTCGCGGGCCCGTGTGATTGCATCGGCGCCGCGACCGGCTGCCCGACTGTGCTTTCCGCATTGCGCCCGCAG
CGCTATTCTTTCCCTCACCA

Product: (dimethylallyl)adenosine tRNA methylthiotransferase

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase [H]

Number of amino acids: Translated: 406; Mature: 406

Protein sequence:

>406_residues
MEITENPAEADVILLNTCSIREKAQEKVFSQLGRWRELKQDNPQLVIGVGGCVASQEGAAIRDRAPYVDVVFGPQTLHRL
PEMIDAARTTRTPQVDISFPEIEKFDRLPEPRVDGPSAYVSVMEGCSKYCTFCVVPYTRGEEVSRPLADVLAEIVHLAEN
GVKEVTLLGQNVNGYRHDGHDFADLLHAVAAIDGIGRIRYTTSHPLEFSDAIIQAHADIPQLVKYLHLPVQAGSDRILAA
MKRNHTALEYKSRIRRLKAAVPDILISSDFIVGFPGETDKDFEQTMKLIEDVGFDFSYSFVYSARPGTPAADLADDTPEE
VKKQRLAILQQRINQQGFENSRRMVGTTQRILVSDYSKKDPGMLQGRTEHNRIVNFRCDNPRLIGQFVDVHIDDALPHSL
RGSLLS

Sequences:

>Translated_406_residues
MEITENPAEADVILLNTCSIREKAQEKVFSQLGRWRELKQDNPQLVIGVGGCVASQEGAAIRDRAPYVDVVFGPQTLHRL
PEMIDAARTTRTPQVDISFPEIEKFDRLPEPRVDGPSAYVSVMEGCSKYCTFCVVPYTRGEEVSRPLADVLAEIVHLAEN
GVKEVTLLGQNVNGYRHDGHDFADLLHAVAAIDGIGRIRYTTSHPLEFSDAIIQAHADIPQLVKYLHLPVQAGSDRILAA
MKRNHTALEYKSRIRRLKAAVPDILISSDFIVGFPGETDKDFEQTMKLIEDVGFDFSYSFVYSARPGTPAADLADDTPEE
VKKQRLAILQQRINQQGFENSRRMVGTTQRILVSDYSKKDPGMLQGRTEHNRIVNFRCDNPRLIGQFVDVHIDDALPHSL
RGSLLS
>Mature_406_residues
MEITENPAEADVILLNTCSIREKAQEKVFSQLGRWRELKQDNPQLVIGVGGCVASQEGAAIRDRAPYVDVVFGPQTLHRL
PEMIDAARTTRTPQVDISFPEIEKFDRLPEPRVDGPSAYVSVMEGCSKYCTFCVVPYTRGEEVSRPLADVLAEIVHLAEN
GVKEVTLLGQNVNGYRHDGHDFADLLHAVAAIDGIGRIRYTTSHPLEFSDAIIQAHADIPQLVKYLHLPVQAGSDRILAA
MKRNHTALEYKSRIRRLKAAVPDILISSDFIVGFPGETDKDFEQTMKLIEDVGFDFSYSFVYSARPGTPAADLADDTPEE
VKKQRLAILQQRINQQGFENSRRMVGTTQRILVSDYSKKDPGMLQGRTEHNRIVNFRCDNPRLIGQFVDVHIDDALPHSL
RGSLLS

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine [H]

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI28872784, Length=446, Percent_Identity=36.9955156950673, Blast_Score=269, Evalue=3e-72,
Organism=Homo sapiens, GI28872782, Length=446, Percent_Identity=36.9955156950673, Blast_Score=268, Evalue=6e-72,
Organism=Homo sapiens, GI93277076, Length=419, Percent_Identity=27.6849642004773, Blast_Score=160, Evalue=2e-39,
Organism=Escherichia coli, GI1786882, Length=409, Percent_Identity=69.1931540342298, Blast_Score=587, Evalue=1e-169,
Organism=Escherichia coli, GI1787057, Length=362, Percent_Identity=28.7292817679558, Blast_Score=150, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI17553146, Length=437, Percent_Identity=33.1807780320366, Blast_Score=231, Evalue=4e-61,
Organism=Caenorhabditis elegans, GI71996771, Length=254, Percent_Identity=30.7086614173228, Blast_Score=116, Evalue=3e-26,
Organism=Drosophila melanogaster, GI21356207, Length=449, Percent_Identity=32.9621380846325, Blast_Score=248, Evalue=7e-66,
Organism=Drosophila melanogaster, GI19922432, Length=412, Percent_Identity=26.4563106796117, Blast_Score=127, Evalue=1e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197
- InterPro:   IPR002792 [H]

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004 [H]

EC number: NA

Molecular weight: Translated: 45376; Mature: 45376

Theoretical pI: Translated: 5.95; Mature: 5.95

Prosite motif: PS50926 TRAM ; PS01278 UPF0004

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEITENPAEADVILLNTCSIREKAQEKVFSQLGRWRELKQDNPQLVIGVGGCVASQEGAA
CCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCEECCCCCCC
IRDRAPYVDVVFGPQTLHRLPEMIDAARTTRTPQVDISFPEIEKFDRLPEPRVDGPSAYV
CCCCCCEEEEEECHHHHHHHHHHHHHHHHCCCCEEECCCCHHHHHHCCCCCCCCCHHHHH
SVMEGCSKYCTFCVVPYTRGEEVSRPLADVLAEIVHLAENGVKEVTLLGQNVNGYRHDGH
HHHHHHHHHCEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCH
DFADLLHAVAAIDGIGRIRYTTSHPLEFSDAIIQAHADIPQLVKYLHLPVQAGSDRILAA
HHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHCCHHHHHHHHHCCCCCCCCHHHHH
MKRNHTALEYKSRIRRLKAAVPDILISSDFIVGFPGETDKDFEQTMKLIEDVGFDFSYSF
HHHCCHHHHHHHHHHHHHHHHCHHEECCCEEEECCCCCCHHHHHHHHHHHHCCCCEEEEE
VYSARPGTPAADLADDTPEEVKKQRLAILQQRINQQGFENSRRMVGTTQRILVSDYSKKD
EEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCHHHHHHHCCCCCC
PGMLQGRTEHNRIVNFRCDNPRLIGQFVDVHIDDALPHSLRGSLLS
CCCCCCCCCCCEEEEEECCCCCEEEHHHHEEECCCCCHHHHHCCCC
>Mature Secondary Structure
MEITENPAEADVILLNTCSIREKAQEKVFSQLGRWRELKQDNPQLVIGVGGCVASQEGAA
CCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCEECCCCCCC
IRDRAPYVDVVFGPQTLHRLPEMIDAARTTRTPQVDISFPEIEKFDRLPEPRVDGPSAYV
CCCCCCEEEEEECHHHHHHHHHHHHHHHHCCCCEEECCCCHHHHHHCCCCCCCCCHHHHH
SVMEGCSKYCTFCVVPYTRGEEVSRPLADVLAEIVHLAENGVKEVTLLGQNVNGYRHDGH
HHHHHHHHHCEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCH
DFADLLHAVAAIDGIGRIRYTTSHPLEFSDAIIQAHADIPQLVKYLHLPVQAGSDRILAA
HHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHCCHHHHHHHHHCCCCCCCCHHHHH
MKRNHTALEYKSRIRRLKAAVPDILISSDFIVGFPGETDKDFEQTMKLIEDVGFDFSYSF
HHHCCHHHHHHHHHHHHHHHHCHHEECCCEEEECCCCCCHHHHHHHHHHHHCCCCEEEEE
VYSARPGTPAADLADDTPEEVKKQRLAILQQRINQQGFENSRRMVGTTQRILVSDYSKKD
EEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCHHHHHHHCCCCCC
PGMLQGRTEHNRIVNFRCDNPRLIGQFVDVHIDDALPHSLRGSLLS
CCCCCCCCCCCEEEEEECCCCCEEEHHHHEEECCCCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA