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The map label for this gene is braD [H]

Identifier: 146284033

GI number: 146284033

Start: 4021258

End: 4022829

Strand: Direct

Name: braD [H]

Synonym: PST_3721

Alternate gene names: 146284033

Gene position: 4021258-4022829 (Clockwise)

Preceding gene: 146284032

Following gene: 146284034

Centisome position: 88.04

GC content: 68.32

Gene sequence:

>1572_bases
ATGAACACTGCCCTGCACCGATTATTGCTGCTGCTCCTGCTGGTTCTGCCCTGGGCAGCCCAGGCCGGAGACGCGGCCGA
CTACGCCAAGGCCAGCCCGGCCAAACAGGCCAGGCTGCTGGAAAGCTGGGCCGCCGCGCCCGCGCCTGAACGGCTGCCGC
TGATCGAAGCGCTGCGCGCCAATCGCGTGGCCATCGACCAGAACAAGACGCCCTTCATCGAGGAAAACGGGGCCTACCGT
GCCCTCGAAGGTGATGCCGAGCCGGTCGGCACGCCGAAGAAGCTGCGTCTGAACAACCGCCTGCGCGGCCTGCTCAACAC
CGCCCTGGCGAGCCACCAGCTGTTCGCCGAAGACCCCGCGCAGCGCCTCGCCGCGGCCAAGCGCCTGCAGCGCAGCGGCC
AACCGGAGCAGCTCGAGCTGCTTCAGCAGCGCCTGGACGCCGAGGACGATGCCGCCGTCAGCGAAGCGCTGACCCTGGCG
CTGGCCAACCTGCAGCTGACCGCGAATGATCCGGCAGTACGCCTCGCCGCGGTGCGCCTGCTCGGCGAAACCGGCGCGCC
CATGGCGCGGGTGCGCCTGGAGAACCTGCTGGCCGACGAGGCGGAAACCGACGCCAACGTGCGCAAGGCCGCCGAGACCA
GCCTGGCGCAGGTCAAGCGCAAGCTCCTGATCGGCGAACTGCTCGGCCAGGCTTTCAGCGGCCTGTCGCTGGGCTCGATC
CTGCTGCTCGCCGCCCTCGGCCTGGCGATCACCTTCGGCCTGCTCGGGGTGATCAACATGGCCCACGGCGAGATGCTCAT
GCTCGGGGCCTACACCACCTACATGGTGCAAGTGCTGATGGCGCGGCTCGCCCCGGAAGCCCTCGCCTTCTACCCGCTGG
TGGCGTTGCCGGTGGCCTTCTTCGTCACCGCCGCGATCGGCATGGCGCTGGAGCGCACGGTGATCCGCCATCTGTACGGC
CGCCCGCTGGAAACCCTGCTGGCCACCTGGGGCATCAGCCTGATCCTGATCCAGCTGGTGCGGGTGCTGTTCGGCGCGCA
GAACGTCGAGGTCGCCAACCCGGCCTGGCTGTCCGGTGGCCTGCAGGTGCTGCCGAACCTGGTGCTGCCGTACAACCGCA
TGGTGATCATCGGCTTCGCGCTGTTCGTGGTGCTCCTGACCTGGCTCTTGCTGAACAAGACGCGCCTGGGCCTGAACGTG
CGCGCGGTCACGCAGAACCGCAACATGGCCGCCTGCTGCGGCGTGCCCACCGGGCGCATCGACATGATGGCCTTCGGGCT
GGGTTCCGGCATCGCCGGGCTCGGCGGCGTGGCCCTGAGCCAGATCGGCAATGTCGGCCCGGACCTCGGCCAGAGCTACA
TCATCGATTCCTTCCTGGTGGTGGTGCTCGGCGGTGTCGGCCAGCTGGCCGGCAGCATCTTCGCCGCCTTCGGCCTCGGC
ATCGCCAACAAGATCCTCGAACCGCAGATCGGCGCCGTGCTGGGCAAGATCCTCATCCTCGCGCTGATCATCCTCTTCAT
CCAGAAGCGTCCGCAGGGGCTCTTCGCTCTCAAGGGGAGGGTCATCGATTGA

Upstream 100 bases:

>100_bases
TTGGGCGGATCGGGACGCGTAGTCGAGACGTTCTCATCCGCCCGCCCATCGCCGCCACCTCCGTCAAGAGAGCCCGCGGC
GCCCCTTGGGGAATTCACGC

Downstream 100 bases:

>100_bases
ACCAGCCACTTACCCTCACCGCCGCGCAGAAAGCCGGGCCGAAAGCCACGGTCATCGCCCTCGGCCTGGCGCTCGCCGTC
CTCGTCGCCATGCCGCTGCT

Product: branched-chain amino acid ABC transporter, permease protein

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 523; Mature: 523

Protein sequence:

>523_residues
MNTALHRLLLLLLLVLPWAAQAGDAADYAKASPAKQARLLESWAAAPAPERLPLIEALRANRVAIDQNKTPFIEENGAYR
ALEGDAEPVGTPKKLRLNNRLRGLLNTALASHQLFAEDPAQRLAAAKRLQRSGQPEQLELLQQRLDAEDDAAVSEALTLA
LANLQLTANDPAVRLAAVRLLGETGAPMARVRLENLLADEAETDANVRKAAETSLAQVKRKLLIGELLGQAFSGLSLGSI
LLLAALGLAITFGLLGVINMAHGEMLMLGAYTTYMVQVLMARLAPEALAFYPLVALPVAFFVTAAIGMALERTVIRHLYG
RPLETLLATWGISLILIQLVRVLFGAQNVEVANPAWLSGGLQVLPNLVLPYNRMVIIGFALFVVLLTWLLLNKTRLGLNV
RAVTQNRNMAACCGVPTGRIDMMAFGLGSGIAGLGGVALSQIGNVGPDLGQSYIIDSFLVVVLGGVGQLAGSIFAAFGLG
IANKILEPQIGAVLGKILILALIILFIQKRPQGLFALKGRVID

Sequences:

>Translated_523_residues
MNTALHRLLLLLLLVLPWAAQAGDAADYAKASPAKQARLLESWAAAPAPERLPLIEALRANRVAIDQNKTPFIEENGAYR
ALEGDAEPVGTPKKLRLNNRLRGLLNTALASHQLFAEDPAQRLAAAKRLQRSGQPEQLELLQQRLDAEDDAAVSEALTLA
LANLQLTANDPAVRLAAVRLLGETGAPMARVRLENLLADEAETDANVRKAAETSLAQVKRKLLIGELLGQAFSGLSLGSI
LLLAALGLAITFGLLGVINMAHGEMLMLGAYTTYMVQVLMARLAPEALAFYPLVALPVAFFVTAAIGMALERTVIRHLYG
RPLETLLATWGISLILIQLVRVLFGAQNVEVANPAWLSGGLQVLPNLVLPYNRMVIIGFALFVVLLTWLLLNKTRLGLNV
RAVTQNRNMAACCGVPTGRIDMMAFGLGSGIAGLGGVALSQIGNVGPDLGQSYIIDSFLVVVLGGVGQLAGSIFAAFGLG
IANKILEPQIGAVLGKILILALIILFIQKRPQGLFALKGRVID
>Mature_523_residues
MNTALHRLLLLLLLVLPWAAQAGDAADYAKASPAKQARLLESWAAAPAPERLPLIEALRANRVAIDQNKTPFIEENGAYR
ALEGDAEPVGTPKKLRLNNRLRGLLNTALASHQLFAEDPAQRLAAAKRLQRSGQPEQLELLQQRLDAEDDAAVSEALTLA
LANLQLTANDPAVRLAAVRLLGETGAPMARVRLENLLADEAETDANVRKAAETSLAQVKRKLLIGELLGQAFSGLSLGSI
LLLAALGLAITFGLLGVINMAHGEMLMLGAYTTYMVQVLMARLAPEALAFYPLVALPVAFFVTAAIGMALERTVIRHLYG
RPLETLLATWGISLILIQLVRVLFGAQNVEVANPAWLSGGLQVLPNLVLPYNRMVIIGFALFVVLLTWLLLNKTRLGLNV
RAVTQNRNMAACCGVPTGRIDMMAFGLGSGIAGLGGVALSQIGNVGPDLGQSYIIDSFLVVVLGGVGQLAGSIFAAFGLG
IANKILEPQIGAVLGKILILALIILFIQKRPQGLFALKGRVID

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG0559

COG function: function code E; Branched-chain amino acid ABC-type transport system, permease components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789866, Length=293, Percent_Identity=26.962457337884, Blast_Score=89, Evalue=6e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851 [H]

Pfam domain/function: PF02653 BPD_transp_2 [H]

EC number: NA

Molecular weight: Translated: 55744; Mature: 55744

Theoretical pI: Translated: 9.67; Mature: 9.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTALHRLLLLLLLVLPWAAQAGDAADYAKASPAKQARLLESWAAAPAPERLPLIEALRA
CCHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCHHHHHHHHHHHHCCCCCCCCHHHHHHHC
NRVAIDQNKTPFIEENGAYRALEGDAEPVGTPKKLRLNNRLRGLLNTALASHQLFAEDPA
CCEEECCCCCCCEECCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHH
QRLAAAKRLQRSGQPEQLELLQQRLDAEDDAAVSEALTLALANLQLTANDPAVRLAAVRL
HHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHEEEECCCCHHHHHHHHH
LGETGAPMARVRLENLLADEAETDANVRKAAETSLAQVKRKLLIGELLGQAFSGLSLGSI
HHCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
LLLAALGLAITFGLLGVINMAHGEMLMLGAYTTYMVQVLMARLAPEALAFYPLVALPVAF
HHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHH
FVTAAIGMALERTVIRHLYGRPLETLLATWGISLILIQLVRVLFGAQNVEVANPAWLSGG
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCC
LQVLPNLVLPYNRMVIIGFALFVVLLTWLLLNKTRLGLNVRAVTQNRNMAACCGVPTGRI
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCEEEECCCCCCCH
DMMAFGLGSGIAGLGGVALSQIGNVGPDLGQSYIIDSFLVVVLGGVGQLAGSIFAAFGLG
HHHHHHHCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
IANKILEPQIGAVLGKILILALIILFIQKRPQGLFALKGRVID
HHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCC
>Mature Secondary Structure
MNTALHRLLLLLLLVLPWAAQAGDAADYAKASPAKQARLLESWAAAPAPERLPLIEALRA
CCHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCHHHHHHHHHHHHCCCCCCCCHHHHHHHC
NRVAIDQNKTPFIEENGAYRALEGDAEPVGTPKKLRLNNRLRGLLNTALASHQLFAEDPA
CCEEECCCCCCCEECCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHH
QRLAAAKRLQRSGQPEQLELLQQRLDAEDDAAVSEALTLALANLQLTANDPAVRLAAVRL
HHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHEEEECCCCHHHHHHHHH
LGETGAPMARVRLENLLADEAETDANVRKAAETSLAQVKRKLLIGELLGQAFSGLSLGSI
HHCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
LLLAALGLAITFGLLGVINMAHGEMLMLGAYTTYMVQVLMARLAPEALAFYPLVALPVAF
HHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHH
FVTAAIGMALERTVIRHLYGRPLETLLATWGISLILIQLVRVLFGAQNVEVANPAWLSGG
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCC
LQVLPNLVLPYNRMVIIGFALFVVLLTWLLLNKTRLGLNVRAVTQNRNMAACCGVPTGRI
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCEEEECCCCCCCH
DMMAFGLGSGIAGLGGVALSQIGNVGPDLGQSYIIDSFLVVVLGGVGQLAGSIFAAFGLG
HHHHHHHCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
IANKILEPQIGAVLGKILILALIILFIQKRPQGLFALKGRVID
HHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043; 2509433 [H]