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The map label for this gene is ppa-1 [H]

Identifier: 146283998

GI number: 146283998

Start: 3984843

End: 3985370

Strand: Direct

Name: ppa-1 [H]

Synonym: PST_3686

Alternate gene names: 146283998

Gene position: 3984843-3985370 (Clockwise)

Preceding gene: 146283997

Following gene: 146284000

Centisome position: 87.24

GC content: 61.55

Gene sequence:

>528_bases
ATGAGCTACAGCAAAGTCCCGGCTGGCAAAGACCTGCCGAACGACATCTACGTCGCCATCGAGATCCCGGCCAACCATGC
GCCGATCAAGTACGAGATCGATCACGACACCGACTGCCTGTTCATCGACCGCTTCATGGCCACCCCGATGTTCTACCCGG
CCAACTACGGTTTCATCCCGCATACCCTGGCCGATGACGGCGACCCCCTCGACGTGCTGGTCGTGACCCCCTATCCGGTT
GCCCCGGGCTCGGTCATCCGCTGCCGTCCGGTCGGCGTGCTGAACATGACCGACGAAGCCGGCGGTGACGCCAAGCTGAT
CGCCGTCCCGCACGACAAGCTGACCCAGCTGTACAAGGACGTGAAGGAATACACCGACCTGCCGCCGCTGCTGATCGAGC
AGATCAAGCACTTCTTCGAGAACTACAAGGATCTCGAGAAGGGCAAGTGGGTCAAGGTCGAAGGCTGGGAAGGCGCCGAC
GCCGCCCGTGCCGCCATCATCAAAGCTGCCGAGGCTTACCAGAAGTAA

Upstream 100 bases:

>100_bases
CGTCGAAGCCGGCGTGCATTGCGCGCGACATTGCGCCTATAATCGCGCCCACTTTTTTTCCGCCCCCAGGCCGCGCTGGC
CGATCAGGAGCCCATCGTTC

Downstream 100 bases:

>100_bases
GTCGAGGCACTGAAGAAAAGCCGGCCACAAGCCGGCTTTTTAATGCCCGCAAATCGGCCTTTTGCCACCAGCCCCGCTTC
GACTATCGCTAGCCCCGCAC

Product: inorganic pyrophosphatase

Products: NA

Alternate protein names: Pyrophosphate phospho-hydrolase 1; PPase 1 [H]

Number of amino acids: Translated: 175; Mature: 174

Protein sequence:

>175_residues
MSYSKVPAGKDLPNDIYVAIEIPANHAPIKYEIDHDTDCLFIDRFMATPMFYPANYGFIPHTLADDGDPLDVLVVTPYPV
APGSVIRCRPVGVLNMTDEAGGDAKLIAVPHDKLTQLYKDVKEYTDLPPLLIEQIKHFFENYKDLEKGKWVKVEGWEGAD
AARAAIIKAAEAYQK

Sequences:

>Translated_175_residues
MSYSKVPAGKDLPNDIYVAIEIPANHAPIKYEIDHDTDCLFIDRFMATPMFYPANYGFIPHTLADDGDPLDVLVVTPYPV
APGSVIRCRPVGVLNMTDEAGGDAKLIAVPHDKLTQLYKDVKEYTDLPPLLIEQIKHFFENYKDLEKGKWVKVEGWEGAD
AARAAIIKAAEAYQK
>Mature_174_residues
SYSKVPAGKDLPNDIYVAIEIPANHAPIKYEIDHDTDCLFIDRFMATPMFYPANYGFIPHTLADDGDPLDVLVVTPYPVA
PGSVIRCRPVGVLNMTDEAGGDAKLIAVPHDKLTQLYKDVKEYTDLPPLLIEQIKHFFENYKDLEKGKWVKVEGWEGADA
ARAAIIKAAEAYQK

Specific function: Unknown

COG id: COG0221

COG function: function code C; Inorganic pyrophosphatase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PPase family [H]

Homologues:

Organism=Escherichia coli, GI1790673, Length=171, Percent_Identity=70.7602339181287, Blast_Score=252, Evalue=1e-68,

Paralogues:

None

Copy number: 5480 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 200 Molecules/Cell In: Early Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008162 [H]

Pfam domain/function: PF00719 Pyrophosphatase [H]

EC number: =3.6.1.1 [H]

Molecular weight: Translated: 19522; Mature: 19390

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: PS00387 PPASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYSKVPAGKDLPNDIYVAIEIPANHAPIKYEIDHDTDCLFIDRFMATPMFYPANYGFIP
CCCCCCCCCCCCCCCEEEEEEECCCCCCEEEEECCCCCEEEEEHHHHCCEECCCCCCCCC
HTLADDGDPLDVLVVTPYPVAPGSVIRCRPVGVLNMTDEAGGDAKLIAVPHDKLTQLYKD
CCCCCCCCCEEEEEECCCCCCCCCEEEEECCEEEECCCCCCCCEEEEEECHHHHHHHHHH
VKEYTDLPPLLIEQIKHFFENYKDLEKGKWVKVEGWEGADAARAAIIKAAEAYQK
HHHHCCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SYSKVPAGKDLPNDIYVAIEIPANHAPIKYEIDHDTDCLFIDRFMATPMFYPANYGFIP
CCCCCCCCCCCCCCEEEEEEECCCCCCEEEEECCCCCEEEEEHHHHCCEECCCCCCCCC
HTLADDGDPLDVLVVTPYPVAPGSVIRCRPVGVLNMTDEAGGDAKLIAVPHDKLTQLYKD
CCCCCCCCCEEEEEECCCCCCCCCEEEEECCEEEECCCCCCCCEEEEEECHHHHHHHHHH
VKEYTDLPPLLIEQIKHFFENYKDLEKGKWVKVEGWEGADAARAAIIKAAEAYQK
HHHHCCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12928499 [H]