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The map label for this gene is malS [H]

Identifier: 146283804

GI number: 146283804

Start: 3774264

End: 3776336

Strand: Direct

Name: malS [H]

Synonym: PST_3487

Alternate gene names: 146283804

Gene position: 3774264-3776336 (Clockwise)

Preceding gene: 146283798

Following gene: 146283805

Centisome position: 82.63

GC content: 68.84

Gene sequence:

>2073_bases
ATGCGTGCCCACCGCCTACCCGCCCTGTCGCTGCTGCTCGGATTCACCCTAGCGCTGCCCGTACTGGCCGATCCCGAGCT
GGCTGCCCGGCTGGATGGCCAGCCGCTGCCGTTGAACTGGAATACCCTGGCCAGCGACCGCTACGACATCGAGCTCAGCC
TCGCGCCCGGCCAGTTGCAGTTGCGCATGCCCCAGGCCAGTGGCGAGGCCGTGGCCCTTGCGCCCTTTCGCCGTCAGCCG
CTTGGCACAGACAGCATTTACCGCTACGAAGTGCCTGAAGCCGGACGCTATCGGCTGATCGTCGAGACCGGCGCCGCGCC
GGCCCTGCGCCTGCTGCCGATCAAGGCCGCCGAGCCGCAGGCAGCCACCGCGGTCTGCAAGCCATGGGAGGGCGGCGCAG
TGGAGGTGACCGTTGGCGAGGTGTTCCGCGACGGCGAGACCCTGCGCGACGCACTGTCCGGCGCCACGGCGGTGGTCCGC
GACGGCCGGGTCAGGCTGCAGCCCGCCGCCGGCAGTGACGGTCTGCTGCTGCTCGAACGCGCCGAAGCGCCCGAGCAGCC
GGTCGCACGCGACTGGCGCAACGCGATCGTCTACTTCGTGCTCACCGACCGCTTCGCCAATGGCGACCCAGGCAACGACC
GCAGCTATGGCCGCGCACCGGATGGCGCCGAGGAGATCGGCACCTTCCACGGCGGCGACCTCAAGGGGCTGACCGAACGG
CTCGACCATATCGCCAGCCTCGGCGTCGACGCGCTGTGGATCAGCGCACCCTACGAGCAGATCCACGGCTGGGTCGGCGG
CGGTGATCGCGGCGACTTTCGCCACTACGGTTACCACGGCTACTACGCGCTCGACTTCACTCAGCTCGACGCCAACATGG
GCAGCGAAGACGACCTGCGCGCGCTCATCAGCGCGGCCCATGCCCGCGGCATCCGCGTGCTGTTCGACGTGGTGCTCAAC
CATCCCGGCTACTCGACCCTGCAGGACATGCAGCAGCTCGGCTTCGGTGCCCTGCGCGACGGCATGGCCGAGTACCTGCC
CGAGCAGTGGACAACCTGGCAGCCGGAAGCCCACGAGAATCTGCACGCCTACCACAACCTGGTGGACTACGAGCATCCGA
GCTGGGCCGCCTGGTGGGGTCGCGACTGGGTGCGGGCGGGCATCGCCGACTACGACACGCCACCCAGCGCCACGGTCGAT
CCGCTCAAGGGCTCGCTCGCCTTCCTGCCAGATTTCCGCACCGAATCCGAGGCGGTCGTCGCGCTGCCGGAGTTTCTCGC
GCACAAGGCGCAGACCCGAGCCGAGCCGCGCGAGGGCTACCGGGTGCGCGATTACCTGATCGAATGGCTGACCGGCTGGG
TGCGCGAGTTCGGCGTCGACGGCTTCCGTGCCGACACCGTCAAGCATGTCGAACCCTCGACCTGGGCCGAGCTGCGCGTG
GCGGCCGAGCGCGCCCGCGCTGACTGGGCCCGCGCCAACCCGGACGATCCCATGGCCGACGAACCGTTCTGGATGGTCGG
CGAAGTGTTCGGTCACGGCCCCGAGGCCAGCGACTATCTCGACCAGGGTTTCGATGCGCTGATCAATTTCGACTTCCAGA
AGCAGGCCGTAGCCGCCAGCGACTGCCTGGCTGCCGCCGAGCCCAGTTACGCCGACTATGCCAGGCGCCTGGCCGAAACG
CCCGGGCACAACCTGCTGAGCTATGCCTCGTCGCATGACACCGCGCTGTTCAACCAGCTGGCCAAGGGCGACCTGGCGCG
TCAGCGCGGGCTCGCCACCGCCCTGCTGCTGGCGCCCGGCGCGGTGCAGATCTACTACGGCGACGAAAGCGCCCGCAGCT
TCGGCCCCAGCGGTTCGGACCCGTATCAGGGCACGCGCAGCTCGATGAACTGGGCCGAGCACCAGCGACCCGAGATCGCC
GGATTGATCGAGCACTGGCAGCGGATTGGTCAGTTCCGTACGCGCCATCCAGCCATCGGCGCGGGTGAACACAAACTGCT
TTCTTCCGGCCAGCCCTATGCCTTCGCCCGCGAGCTGGGCGAGGACAAGGTCATCGTGGTGCAGGCCCGATGA

Upstream 100 bases:

>100_bases
CGCAAGCGCACAAAATACGCCATCAGCCTAGCGCTTACGCCTATACCTGGCAGCCAACGCGCAGTCAAATGTGGCAAAAA
AGCATAATGGAGCCACTTCG

Downstream 100 bases:

>100_bases
GTACCGCATCCCTGACCGCAGCGGCACGGCGCCAGGCCCAGCTGGCCTTTCTCGCCAGCGGCACCGAGTTTCGCCTGGGC
CGCGCGGAGGATTGCCCGCT

Product: periplasmic alpha-amylase precursor

Products: NA

Alternate protein names: 1,4-alpha-D-glucan glucanohydrolase [H]

Number of amino acids: Translated: 690; Mature: 690

Protein sequence:

>690_residues
MRAHRLPALSLLLGFTLALPVLADPELAARLDGQPLPLNWNTLASDRYDIELSLAPGQLQLRMPQASGEAVALAPFRRQP
LGTDSIYRYEVPEAGRYRLIVETGAAPALRLLPIKAAEPQAATAVCKPWEGGAVEVTVGEVFRDGETLRDALSGATAVVR
DGRVRLQPAAGSDGLLLLERAEAPEQPVARDWRNAIVYFVLTDRFANGDPGNDRSYGRAPDGAEEIGTFHGGDLKGLTER
LDHIASLGVDALWISAPYEQIHGWVGGGDRGDFRHYGYHGYYALDFTQLDANMGSEDDLRALISAAHARGIRVLFDVVLN
HPGYSTLQDMQQLGFGALRDGMAEYLPEQWTTWQPEAHENLHAYHNLVDYEHPSWAAWWGRDWVRAGIADYDTPPSATVD
PLKGSLAFLPDFRTESEAVVALPEFLAHKAQTRAEPREGYRVRDYLIEWLTGWVREFGVDGFRADTVKHVEPSTWAELRV
AAERARADWARANPDDPMADEPFWMVGEVFGHGPEASDYLDQGFDALINFDFQKQAVAASDCLAAAEPSYADYARRLAET
PGHNLLSYASSHDTALFNQLAKGDLARQRGLATALLLAPGAVQIYYGDESARSFGPSGSDPYQGTRSSMNWAEHQRPEIA
GLIEHWQRIGQFRTRHPAIGAGEHKLLSSGQPYAFARELGEDKVIVVQAR

Sequences:

>Translated_690_residues
MRAHRLPALSLLLGFTLALPVLADPELAARLDGQPLPLNWNTLASDRYDIELSLAPGQLQLRMPQASGEAVALAPFRRQP
LGTDSIYRYEVPEAGRYRLIVETGAAPALRLLPIKAAEPQAATAVCKPWEGGAVEVTVGEVFRDGETLRDALSGATAVVR
DGRVRLQPAAGSDGLLLLERAEAPEQPVARDWRNAIVYFVLTDRFANGDPGNDRSYGRAPDGAEEIGTFHGGDLKGLTER
LDHIASLGVDALWISAPYEQIHGWVGGGDRGDFRHYGYHGYYALDFTQLDANMGSEDDLRALISAAHARGIRVLFDVVLN
HPGYSTLQDMQQLGFGALRDGMAEYLPEQWTTWQPEAHENLHAYHNLVDYEHPSWAAWWGRDWVRAGIADYDTPPSATVD
PLKGSLAFLPDFRTESEAVVALPEFLAHKAQTRAEPREGYRVRDYLIEWLTGWVREFGVDGFRADTVKHVEPSTWAELRV
AAERARADWARANPDDPMADEPFWMVGEVFGHGPEASDYLDQGFDALINFDFQKQAVAASDCLAAAEPSYADYARRLAET
PGHNLLSYASSHDTALFNQLAKGDLARQRGLATALLLAPGAVQIYYGDESARSFGPSGSDPYQGTRSSMNWAEHQRPEIA
GLIEHWQRIGQFRTRHPAIGAGEHKLLSSGQPYAFARELGEDKVIVVQAR
>Mature_690_residues
MRAHRLPALSLLLGFTLALPVLADPELAARLDGQPLPLNWNTLASDRYDIELSLAPGQLQLRMPQASGEAVALAPFRRQP
LGTDSIYRYEVPEAGRYRLIVETGAAPALRLLPIKAAEPQAATAVCKPWEGGAVEVTVGEVFRDGETLRDALSGATAVVR
DGRVRLQPAAGSDGLLLLERAEAPEQPVARDWRNAIVYFVLTDRFANGDPGNDRSYGRAPDGAEEIGTFHGGDLKGLTER
LDHIASLGVDALWISAPYEQIHGWVGGGDRGDFRHYGYHGYYALDFTQLDANMGSEDDLRALISAAHARGIRVLFDVVLN
HPGYSTLQDMQQLGFGALRDGMAEYLPEQWTTWQPEAHENLHAYHNLVDYEHPSWAAWWGRDWVRAGIADYDTPPSATVD
PLKGSLAFLPDFRTESEAVVALPEFLAHKAQTRAEPREGYRVRDYLIEWLTGWVREFGVDGFRADTVKHVEPSTWAELRV
AAERARADWARANPDDPMADEPFWMVGEVFGHGPEASDYLDQGFDALINFDFQKQAVAASDCLAAAEPSYADYARRLAET
PGHNLLSYASSHDTALFNQLAKGDLARQRGLATALLLAPGAVQIYYGDESARSFGPSGSDPYQGTRSSMNWAEHQRPEIA
GLIEHWQRIGQFRTRHPAIGAGEHKLLSSGQPYAFARELGEDKVIVVQAR

Specific function: Since only maltooligosaccharides up to a chain length of 6 glucose units are actively transported through the cytoplasmic membrane via the membrane-bound complex of three proteins, MalF, MalG, and MalK, longer maltooligosaccharides must first be degraded

COG id: COG0366

COG function: function code G; Glycosidases

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Escherichia coli, GI1789995, Length=601, Percent_Identity=53.4109816971714, Blast_Score=632, Evalue=0.0,
Organism=Escherichia coli, GI1786604, Length=531, Percent_Identity=24.6704331450094, Blast_Score=76, Evalue=8e-15,
Organism=Escherichia coli, GI1790687, Length=130, Percent_Identity=30, Blast_Score=63, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014635
- InterPro:   IPR006047
- InterPro:   IPR006589
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF00128 Alpha-amylase [H]

EC number: =3.2.1.1 [H]

Molecular weight: Translated: 76078; Mature: 76078

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRAHRLPALSLLLGFTLALPVLADPELAARLDGQPLPLNWNTLASDRYDIELSLAPGQLQ
CCCCCCHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCCCCCCCCEEEEEEECCCEEE
LRMPQASGEAVALAPFRRQPLGTDSIYRYEVPEAGRYRLIVETGAAPALRLLPIKAAEPQ
EEECCCCCCEEEECCCCCCCCCCCCEEEEECCCCCCEEEEEECCCCCCEEEEEECCCCCC
AATAVCKPWEGGAVEVTVGEVFRDGETLRDALSGATAVVRDGRVRLQPAAGSDGLLLLER
HHHHEECCCCCCEEEEEHHHHHCCCHHHHHHHCCCCEEEECCEEEEEECCCCCCEEEEEC
AEAPEQPVARDWRNAIVYFVLTDRFANGDPGNDRSYGRAPDGAEEIGTFHGGDLKGLTER
CCCCCCHHHHHHHCEEEEEEEECCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCHHHHHHH
LDHIASLGVDALWISAPYEQIHGWVGGGDRGDFRHYGYHGYYALDFTQLDANMGSEDDLR
HHHHHHCCCCEEEEECCHHHHCCCCCCCCCCCCCCCCCCEEEEEEEEEECCCCCCHHHHH
ALISAAHARGIRVLFDVVLNHPGYSTLQDMQQLGFGALRDGMAEYLPEQWTTWQPEAHEN
HHHHHHHHCHHHHHHHHHHCCCCHHHHHHHHHHCHHHHHHHHHHHCCCCCCCCCCCHHHH
LHAYHNLVDYEHPSWAAWWGRDWVRAGIADYDTPPSATVDPLKGSLAFLPDFRTESEAVV
HHHHHHHHCCCCCCCHHHHCHHHHHHCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCEE
ALPEFLAHKAQTRAEPREGYRVRDYLIEWLTGWVREFGVDGFRADTVKHVEPSTWAELRV
HHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHH
AAERARADWARANPDDPMADEPFWMVGEVFGHGPEASDYLDQGFDALINFDFQKQAVAAS
HHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHCCHHHHCCCCHHHHHHHHH
DCLAAAEPSYADYARRLAETPGHNLLSYASSHDTALFNQLAKGDLARQRGLATALLLAPG
HHHHHCCCCHHHHHHHHHCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHCHHEEEEECCC
AVQIYYGDESARSFGPSGSDPYQGTRSSMNWAEHQRPEIAGLIEHWQRIGQFRTRHPAIG
EEEEEECCCHHHHCCCCCCCCCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHCCCCCC
AGEHKLLSSGQPYAFARELGEDKVIVVQAR
CCCHHHHCCCCCHHHHHHCCCCCEEEEEEC
>Mature Secondary Structure
MRAHRLPALSLLLGFTLALPVLADPELAARLDGQPLPLNWNTLASDRYDIELSLAPGQLQ
CCCCCCHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCCCCCCCCEEEEEEECCCEEE
LRMPQASGEAVALAPFRRQPLGTDSIYRYEVPEAGRYRLIVETGAAPALRLLPIKAAEPQ
EEECCCCCCEEEECCCCCCCCCCCCEEEEECCCCCCEEEEEECCCCCCEEEEEECCCCCC
AATAVCKPWEGGAVEVTVGEVFRDGETLRDALSGATAVVRDGRVRLQPAAGSDGLLLLER
HHHHEECCCCCCEEEEEHHHHHCCCHHHHHHHCCCCEEEECCEEEEEECCCCCCEEEEEC
AEAPEQPVARDWRNAIVYFVLTDRFANGDPGNDRSYGRAPDGAEEIGTFHGGDLKGLTER
CCCCCCHHHHHHHCEEEEEEEECCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCHHHHHHH
LDHIASLGVDALWISAPYEQIHGWVGGGDRGDFRHYGYHGYYALDFTQLDANMGSEDDLR
HHHHHHCCCCEEEEECCHHHHCCCCCCCCCCCCCCCCCCEEEEEEEEEECCCCCCHHHHH
ALISAAHARGIRVLFDVVLNHPGYSTLQDMQQLGFGALRDGMAEYLPEQWTTWQPEAHEN
HHHHHHHHCHHHHHHHHHHCCCCHHHHHHHHHHCHHHHHHHHHHHCCCCCCCCCCCHHHH
LHAYHNLVDYEHPSWAAWWGRDWVRAGIADYDTPPSATVDPLKGSLAFLPDFRTESEAVV
HHHHHHHHCCCCCCCHHHHCHHHHHHCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCEE
ALPEFLAHKAQTRAEPREGYRVRDYLIEWLTGWVREFGVDGFRADTVKHVEPSTWAELRV
HHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHH
AAERARADWARANPDDPMADEPFWMVGEVFGHGPEASDYLDQGFDALINFDFQKQAVAAS
HHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHCCHHHHCCCCHHHHHHHHH
DCLAAAEPSYADYARRLAETPGHNLLSYASSHDTALFNQLAKGDLARQRGLATALLLAPG
HHHHHCCCCHHHHHHHHHCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHCHHEEEEECCC
AVQIYYGDESARSFGPSGSDPYQGTRSSMNWAEHQRPEIAGLIEHWQRIGQFRTRHPAIG
EEEEEECCCHHHHCCCCCCCCCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHCCCCCC
AGEHKLLSSGQPYAFARELGEDKVIVVQAR
CCCHHHHCCCCCHHHHHHCCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1544897; 8041620; 9278503; 9268356 [H]