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The map label for this gene is lpsJ [H]

Identifier: 146283544

GI number: 146283544

Start: 3475701

End: 3476330

Strand: Direct

Name: lpsJ [H]

Synonym: PST_3219

Alternate gene names: 146283544

Gene position: 3475701-3476330 (Clockwise)

Preceding gene: 146283543

Following gene: 146283545

Centisome position: 76.1

GC content: 66.51

Gene sequence:

>630_bases
ATGGCCTGGACACGTGAACAGATGGCGCAACGCGCCGCCCAGGAGCTGCAGGACGGCTTCTACGTCAACCTCGGTATCGG
CCTGCCTACCCTGGTCGCCAACTACATCCCGGAAGGCATGGACGTCTGGCTGCAGAGCGAAAACGGCCTGCTCGGCATCG
GCCCCTTCCCCAGCGAGGAAGCGATCGACCCGGACCTGATCAACGCCGGCAAGCAGACCGTCACCGCCCTGCCCGGCTCG
AGCTTCTTCGACAACGCGCAGAGCTTTGCGATGATCCGCGGCGGTCACATCAACCTGGCCATCCTCGGCGCCATGCAGGT
GTCGGAAAAGGGCGACCTGGCCAACTGGATGATCCCCGGCAAGATGGTCAAGGGCATGGGCGGCGCGATGGACCTGGTGG
CCGGCGTCAAGCGCGTCGTGGTGCTGATGGAGCACACCGCCAAGGGCGGCGCGCACAAGATCCTGCCGGCCTGCGACCTG
CCGCTCACCGGCCTCGGCGTGGTCGACCGGATCATCACTGACCTCGGCGTGCTGGACGTCACCGAGCAAGGCCTGAAGCT
GGTGGAGCTGGCCGAAGGCGTCAGCTTCGACGAGCTGCAAGAGGCCACCGGCTGCCCGATCCAGCGCTGA

Upstream 100 bases:

>100_bases
CCGGACCAGATTCACCTGCCGGGCATCTACGTGCATCGCATCGTGCACAACCCGAACCCGGAAAAACGCATCGAAAAACG
CACGGTGAGGAGCTGAGACC

Downstream 100 bases:

>100_bases
AGCCTGGGTGCGGGAAGCACGCCGGAGCACCCCTTGCCCGCTGCGTCTTCCCGCCTGCCCCGAACGCCCCGAGCCGGCGG
CGCGCCAGCACTGGCCAGTC

Product: CoA transferase, subunit B

Products: NA

Alternate protein names: OXCT B; Succinyl CoA:3-oxoacid CoA-transferase [H]

Number of amino acids: Translated: 209; Mature: 208

Protein sequence:

>209_residues
MAWTREQMAQRAAQELQDGFYVNLGIGLPTLVANYIPEGMDVWLQSENGLLGIGPFPSEEAIDPDLINAGKQTVTALPGS
SFFDNAQSFAMIRGGHINLAILGAMQVSEKGDLANWMIPGKMVKGMGGAMDLVAGVKRVVVLMEHTAKGGAHKILPACDL
PLTGLGVVDRIITDLGVLDVTEQGLKLVELAEGVSFDELQEATGCPIQR

Sequences:

>Translated_209_residues
MAWTREQMAQRAAQELQDGFYVNLGIGLPTLVANYIPEGMDVWLQSENGLLGIGPFPSEEAIDPDLINAGKQTVTALPGS
SFFDNAQSFAMIRGGHINLAILGAMQVSEKGDLANWMIPGKMVKGMGGAMDLVAGVKRVVVLMEHTAKGGAHKILPACDL
PLTGLGVVDRIITDLGVLDVTEQGLKLVELAEGVSFDELQEATGCPIQR
>Mature_208_residues
AWTREQMAQRAAQELQDGFYVNLGIGLPTLVANYIPEGMDVWLQSENGLLGIGPFPSEEAIDPDLINAGKQTVTALPGSS
FFDNAQSFAMIRGGHINLAILGAMQVSEKGDLANWMIPGKMVKGMGGAMDLVAGVKRVVVLMEHTAKGGAHKILPACDLP
LTGLGVVDRIITDLGVLDVTEQGLKLVELAEGVSFDELQEATGCPIQR

Specific function: SHORT-CHAIN FATTY ACIDS METABOLISM. [C]

COG id: COG2057

COG function: function code I; Acyl CoA:acetate/3-ketoacid CoA transferase, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-oxoacid CoA-transferase subunit B family [H]

Homologues:

Organism=Homo sapiens, GI4557817, Length=203, Percent_Identity=62.5615763546798, Blast_Score=264, Evalue=3e-71,
Organism=Homo sapiens, GI11545841, Length=203, Percent_Identity=60.0985221674877, Blast_Score=245, Evalue=2e-65,
Organism=Escherichia coli, GI1788552, Length=199, Percent_Identity=51.7587939698493, Blast_Score=215, Evalue=2e-57,
Organism=Caenorhabditis elegans, GI17531517, Length=207, Percent_Identity=56.0386473429952, Blast_Score=240, Evalue=4e-64,
Organism=Drosophila melanogaster, GI21358325, Length=203, Percent_Identity=59.6059113300493, Blast_Score=256, Evalue=7e-69,
Organism=Drosophila melanogaster, GI24655794, Length=203, Percent_Identity=59.6059113300493, Blast_Score=256, Evalue=9e-69,

Paralogues:

None

Copy number: 369 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012791
- InterPro:   IPR004165
- InterPro:   IPR004164 [H]

Pfam domain/function: PF01144 CoA_trans [H]

EC number: =2.8.3.5 [H]

Molecular weight: Translated: 22214; Mature: 22083

Theoretical pI: Translated: 4.35; Mature: 4.35

Prosite motif: PS01274 COA_TRANSF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAWTREQMAQRAAQELQDGFYVNLGIGLPTLVANYIPEGMDVWLQSENGLLGIGPFPSEE
CCCCHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHCCCCCEEEEECCCCEEEECCCCCCC
AIDPDLINAGKQTVTALPGSSFFDNAQSFAMIRGGHINLAILGAMQVSEKGDLANWMIPG
CCCHHHHHCCCCEEEECCCCHHHHCCHHEEEEECCCEEEEEEEEEEECCCCCCCCCCCCH
KMVKGMGGAMDLVAGVKRVVVLMEHTAKGGAHKILPACDLPLTGLGVVDRIITDLGVLDV
HHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHH
TEQGLKLVELAEGVSFDELQEATGCPIQR
HHHHHHHHHHHCCCCHHHHHHHCCCCCCC
>Mature Secondary Structure 
AWTREQMAQRAAQELQDGFYVNLGIGLPTLVANYIPEGMDVWLQSENGLLGIGPFPSEE
CCCHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHCCCCCEEEEECCCCEEEECCCCCCC
AIDPDLINAGKQTVTALPGSSFFDNAQSFAMIRGGHINLAILGAMQVSEKGDLANWMIPG
CCCHHHHHCCCCEEEECCCCHHHHCCHHEEEEECCCEEEEEEEEEEECCCCCCCCCCCCH
KMVKGMGGAMDLVAGVKRVVVLMEHTAKGGAHKILPACDLPLTGLGVVDRIITDLGVLDV
HHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHH
TEQGLKLVELAEGVSFDELQEATGCPIQR
HHHHHHHHHHHCCCCHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9385134 [H]