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The map label for this gene is intA [H]

Identifier: 146283518

GI number: 146283518

Start: 3437829

End: 3439196

Strand: Direct

Name: intA [H]

Synonym: PST_3193

Alternate gene names: 146283518

Gene position: 3437829-3439196 (Clockwise)

Preceding gene: 146283517

Following gene: 146283519

Centisome position: 75.27

GC content: 59.14

Gene sequence:

>1368_bases
ATGGAATGGGCAGGCCTTTTGAAATCAATAGCTTGCCTAACAATACGGTCTGCTGAGCGTTCCTATTGTTCATCGAGTTC
TGAAAAAATCGGGGGTAGCGTTGGGGGTACTCGTGTTCAACCGTGGGGGTATTTTTCCCCGCAGTGGAGTCCAACGATGC
CCGAGAATCTACTCACCGACGCCAAGATCAAGTCGGTCAAACCCACTGATCGAGACTGGAAACTTTCAGACGGCGGCGGT
TTGTTCCTGCTGGTCAAGCCTACCGGCTGCAAGCTCTGGCGGTGGAAGTATCGCCTGCAAGGCAAGGAAAACCTCTTTGC
CATCGGCAGCTTTCCGCAAGTAAGCCTTGCAGAGGCGCGTACTGCCCGAGAGAAAGCGCGCGCCCTGGTCAAGCAAGGTA
TCCACCCCGCCCATGAGCGGCAGCAGGTTAAGCAGCGCAATCTTGAGGCGCTGGCAGAACGCAAACGCGCCCGGGAAAGC
TCGTTTGCCAAGGTGGCGCAAGCGTACCTGACCGAGATCAAGCCTGTCTTCGCGTACAGCTCCTATCGCACGAAAGAGTC
CCGCATCAGGAAGTACCTGTCTCCCAAGTTCGACGGAATGCCGATGAGCGACATTGGCGTCAAGCAGATCCGCCCACTGC
TCGAAGAGTGTAAGACTCATGGCGCATGGGCTGCGATCCATGTCAAAGGCGACCTTTCGGCCATCTTCGAATTCGCGGTC
GTGCGTGGCCTGGTTGAGGCCAACCCGATCCCGAGCCTGCGCGGGCTGCTGCGCGCACCTGCCAGCGAAAGCAAAGCGGC
GATGACACGCGAGCAGATCCAGAAGTTTTATCAGGCATTGCGCGGCTACAGGGGCTATCCGGAAACTGCGCTGTGCCTGC
GGTTGATTGCGCTGACCGCTTGCCGTCCCGGGGAAGCTGCCGATGCCGAATGGGAAGAGTTCGATTTTGAGGATGCCGTG
TGGCGTCGACCTGCGGCCAAGATGAAGGCACGCCGCGACCACATCAGCCCGCTGTCCACGCAGGCTATCGCTGTACTGAA
AGGCTTGCAGCGCATCACGGGTGGCGGACGCTACTTGTTCCCTCACCGGAGCGGCAAAGGCTTCACCACGCCCAACCGGC
TTACCTACGCCATGCGTGATATGAACCTGGGCCGGGGAACGACGCCCCATTGCTGGCGCACCACCTTTTCGACCTGGGCC
AACGAAAACGGGTTCCGACCCGACGCGATCGAGCGGCAGCTTGCTCATGTGGAAAGCAACAAGGTTCGGGCGACCTACAA
TAAGGCGCTGCTGCTGGATCAGAGAAGGTCGCTACTACAGGCTTGGGCGGGCTACCTGAGTACGGCAGAGCACAGCGGCA
CCGAATAA

Upstream 100 bases:

>100_bases
TGGAAGGCAAGGAGTACATCGTCAAGGACGGCGACGTGATGCACTTCCGCTTCAACGTCTGACGATTTTTTCTGAACTCA
GGAGCGCTCAATAGCGCTCG

Downstream 100 bases:

>100_bases
TGCTGTTCGCCTTTGCGGAGGACTGGAGGCTATAAACAGGGCAATCCAATAATTAAAAAGGGTAATTTAGGCCGCCAATG
CCCCTTTATAGGCCGTGATT

Product: phage integrase family site specific recombinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 455; Mature: 455

Protein sequence:

>455_residues
MEWAGLLKSIACLTIRSAERSYCSSSSEKIGGSVGGTRVQPWGYFSPQWSPTMPENLLTDAKIKSVKPTDRDWKLSDGGG
LFLLVKPTGCKLWRWKYRLQGKENLFAIGSFPQVSLAEARTAREKARALVKQGIHPAHERQQVKQRNLEALAERKRARES
SFAKVAQAYLTEIKPVFAYSSYRTKESRIRKYLSPKFDGMPMSDIGVKQIRPLLEECKTHGAWAAIHVKGDLSAIFEFAV
VRGLVEANPIPSLRGLLRAPASESKAAMTREQIQKFYQALRGYRGYPETALCLRLIALTACRPGEAADAEWEEFDFEDAV
WRRPAAKMKARRDHISPLSTQAIAVLKGLQRITGGGRYLFPHRSGKGFTTPNRLTYAMRDMNLGRGTTPHCWRTTFSTWA
NENGFRPDAIERQLAHVESNKVRATYNKALLLDQRRSLLQAWAGYLSTAEHSGTE

Sequences:

>Translated_455_residues
MEWAGLLKSIACLTIRSAERSYCSSSSEKIGGSVGGTRVQPWGYFSPQWSPTMPENLLTDAKIKSVKPTDRDWKLSDGGG
LFLLVKPTGCKLWRWKYRLQGKENLFAIGSFPQVSLAEARTAREKARALVKQGIHPAHERQQVKQRNLEALAERKRARES
SFAKVAQAYLTEIKPVFAYSSYRTKESRIRKYLSPKFDGMPMSDIGVKQIRPLLEECKTHGAWAAIHVKGDLSAIFEFAV
VRGLVEANPIPSLRGLLRAPASESKAAMTREQIQKFYQALRGYRGYPETALCLRLIALTACRPGEAADAEWEEFDFEDAV
WRRPAAKMKARRDHISPLSTQAIAVLKGLQRITGGGRYLFPHRSGKGFTTPNRLTYAMRDMNLGRGTTPHCWRTTFSTWA
NENGFRPDAIERQLAHVESNKVRATYNKALLLDQRRSLLQAWAGYLSTAEHSGTE
>Mature_455_residues
MEWAGLLKSIACLTIRSAERSYCSSSSEKIGGSVGGTRVQPWGYFSPQWSPTMPENLLTDAKIKSVKPTDRDWKLSDGGG
LFLLVKPTGCKLWRWKYRLQGKENLFAIGSFPQVSLAEARTAREKARALVKQGIHPAHERQQVKQRNLEALAERKRARES
SFAKVAQAYLTEIKPVFAYSSYRTKESRIRKYLSPKFDGMPMSDIGVKQIRPLLEECKTHGAWAAIHVKGDLSAIFEFAV
VRGLVEANPIPSLRGLLRAPASESKAAMTREQIQKFYQALRGYRGYPETALCLRLIALTACRPGEAADAEWEEFDFEDAV
WRRPAAKMKARRDHISPLSTQAIAVLKGLQRITGGGRYLFPHRSGKGFTTPNRLTYAMRDMNLGRGTTPHCWRTTFSTWA
NENGFRPDAIERQLAHVESNKVRATYNKALLLDQRRSLLQAWAGYLSTAEHSGTE

Specific function: Integrase is necessary for integration of the phage into the host genome by site-specific recombination. In conjunction with excisionase, integrase is also necessary for excision of the prophage from the host genome [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'phage' integrase family [H]

Homologues:

Organism=Escherichia coli, GI1788974, Length=407, Percent_Identity=30.7125307125307, Blast_Score=199, Evalue=3e-52,
Organism=Escherichia coli, GI145693166, Length=399, Percent_Identity=33.8345864661654, Blast_Score=196, Evalue=2e-51,
Organism=Escherichia coli, GI1788690, Length=394, Percent_Identity=27.6649746192893, Blast_Score=170, Evalue=2e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011010
- InterPro:   IPR013762
- InterPro:   IPR002104
- InterPro:   IPR023109 [H]

Pfam domain/function: PF00589 Phage_integrase [H]

EC number: NA

Molecular weight: Translated: 51208; Mature: 51208

Theoretical pI: Translated: 10.48; Mature: 10.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEWAGLLKSIACLTIRSAERSYCSSSSEKIGGSVGGTRVQPWGYFSPQWSPTMPENLLTD
CCHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCC
AKIKSVKPTDRDWKLSDGGGLFLLVKPTGCKLWRWKYRLQGKENLFAIGSFPQVSLAEAR
CHHCCCCCCCCCEEECCCCCEEEEEECCCCCEEEEEEEECCCCCEEEECCCCCHHHHHHH
TAREKARALVKQGIHPAHERQQVKQRNLEALAERKRARESSFAKVAQAYLTEIKPVFAYS
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SYRTKESRIRKYLSPKFDGMPMSDIGVKQIRPLLEECKTHGAWAAIHVKGDLSAIFEFAV
HCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHH
VRGLVEANPIPSLRGLLRAPASESKAAMTREQIQKFYQALRGYRGYPETALCLRLIALTA
HHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHC
CRPGEAADAEWEEFDFEDAVWRRPAAKMKARRDHISPLSTQAIAVLKGLQRITGGGRYLF
CCCCCCCCCCHHHCCCHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCEEEC
PHRSGKGFTTPNRLTYAMRDMNLGRGTTPHCWRTTFSTWANENGFRPDAIERQLAHVESN
CCCCCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCC
KVRATYNKALLLDQRRSLLQAWAGYLSTAEHSGTE
CEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MEWAGLLKSIACLTIRSAERSYCSSSSEKIGGSVGGTRVQPWGYFSPQWSPTMPENLLTD
CCHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCC
AKIKSVKPTDRDWKLSDGGGLFLLVKPTGCKLWRWKYRLQGKENLFAIGSFPQVSLAEAR
CHHCCCCCCCCCEEECCCCCEEEEEECCCCCEEEEEEEECCCCCEEEECCCCCHHHHHHH
TAREKARALVKQGIHPAHERQQVKQRNLEALAERKRARESSFAKVAQAYLTEIKPVFAYS
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SYRTKESRIRKYLSPKFDGMPMSDIGVKQIRPLLEECKTHGAWAAIHVKGDLSAIFEFAV
HCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHH
VRGLVEANPIPSLRGLLRAPASESKAAMTREQIQKFYQALRGYRGYPETALCLRLIALTA
HHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHC
CRPGEAADAEWEEFDFEDAVWRRPAAKMKARRDHISPLSTQAIAVLKGLQRITGGGRYLF
CCCCCCCCCCHHHCCCHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCEEEC
PHRSGKGFTTPNRLTYAMRDMNLGRGTTPHCWRTTFSTWANENGFRPDAIERQLAHVESN
CCCCCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCC
KVRATYNKALLLDQRRSLLQAWAGYLSTAEHSGTE
CEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7511583; 9278503; 9205837; 7524073 [H]