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The map label for this gene is rhtB [H]

Identifier: 146283418

GI number: 146283418

Start: 3326102

End: 3326728

Strand: Direct

Name: rhtB [H]

Synonym: PST_3091

Alternate gene names: 146283418

Gene position: 3326102-3326728 (Clockwise)

Preceding gene: 146283417

Following gene: 146283420

Centisome position: 72.82

GC content: 65.07

Gene sequence:

>627_bases
ATGAGTCTGGAAACCTGGTTGTTGTATACGAGTGCGGCGCTGGTGGTGATCCTCATCCCCGGACCGCTGTCGCTGTTGAT
GATCAGCAACAGCCTGAACTACGGAGTGCGCCGCTCCTGGCCAGCCTTTCTCGGCGGCGTGACCGCTTCCATCGCCCTGC
TCAGCGCCTCGGCACTGGGGCTTGGCGCGCTGTTGATGGCCTCGGAGCGCCTGTTCAGCGCGCTCAAGCTGATCGGTGCG
CTGTACCTGTTCTACCTCGCCTGGCAGAGCTGGCAGGCGGCACGCCAGGCACCGGCTGCCCGCGAGATCGAAAGCCCTGC
CCTCGCCCCGCGTTTCAGCAGGCTGTTCGGCCGAGCGTTCATTCTGGGGGGCAGCAACCCGAAGGACATGCTCTTCTTCG
CCGCCTTCCTGCCGCAGTTTCTCAACGCGGGGCAACCGTTGCTACCGCAGCTGCTGGTGATGACCCTGACCTGGGCCATA
CTGGATCTCTGCTGCAAGCTGGCCTATGGGCTGGGAGCGCGGGGAGCGGCGCGCTACCTGCGCACCGGGAAAGGGCATGC
GTGGTTCAACCGGACCAGCGCAGCACTGTTTGGAAGCGCGGGAGCGGCGTCCTTGATGAGTCGGTGA

Upstream 100 bases:

>100_bases
TGCGTGATCTTCATCGCCAAGTTCGCGTTTCTCTGAACGAAGCGCGCCTCACCGCCCCGTCGCATTGCGGCGGGGCTTTT
TCATTTCTCAGGAGACTGTA

Downstream 100 bases:

>100_bases
CTCGGCTGGTGTTGGCAGCGCCGGCTCGCGACCTGATGGAGGGTGACATGCTGCCGCGGTGCAACCTGCAGTCCGACCCG
AGAAACGCGCGGCGCATCCG

Product: amino acid transporter LysE

Products: homoserine lactone [Periplasm]; Proton [Cytoplasm]; homoserine [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 208; Mature: 207

Protein sequence:

>208_residues
MSLETWLLYTSAALVVILIPGPLSLLMISNSLNYGVRRSWPAFLGGVTASIALLSASALGLGALLMASERLFSALKLIGA
LYLFYLAWQSWQAARQAPAAREIESPALAPRFSRLFGRAFILGGSNPKDMLFFAAFLPQFLNAGQPLLPQLLVMTLTWAI
LDLCCKLAYGLGARGAARYLRTGKGHAWFNRTSAALFGSAGAASLMSR

Sequences:

>Translated_208_residues
MSLETWLLYTSAALVVILIPGPLSLLMISNSLNYGVRRSWPAFLGGVTASIALLSASALGLGALLMASERLFSALKLIGA
LYLFYLAWQSWQAARQAPAAREIESPALAPRFSRLFGRAFILGGSNPKDMLFFAAFLPQFLNAGQPLLPQLLVMTLTWAI
LDLCCKLAYGLGARGAARYLRTGKGHAWFNRTSAALFGSAGAASLMSR
>Mature_207_residues
SLETWLLYTSAALVVILIPGPLSLLMISNSLNYGVRRSWPAFLGGVTASIALLSASALGLGALLMASERLFSALKLIGAL
YLFYLAWQSWQAARQAPAAREIESPALAPRFSRLFGRAFILGGSNPKDMLFFAAFLPQFLNAGQPLLPQLLVMTLTWAIL
DLCCKLAYGLGARGAARYLRTGKGHAWFNRTSAALFGSAGAASLMSR

Specific function: Conducts the efflux of homoserine and homoserine lactone [H]

COG id: COG1280

COG function: function code E; Putative threonine efflux protein

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the rht family [H]

Homologues:

Organism=Escherichia coli, GI48994979, Length=202, Percent_Identity=31.6831683168317, Blast_Score=92, Evalue=3e-20,
Organism=Escherichia coli, GI1788099, Length=177, Percent_Identity=25.9887005649718, Blast_Score=75, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004778
- InterPro:   IPR001123 [H]

Pfam domain/function: PF01810 LysE [H]

EC number: NA

Molecular weight: Translated: 22345; Mature: 22213

Theoretical pI: Translated: 10.96; Mature: 10.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLETWLLYTSAALVVILIPGPLSLLMISNSLNYGVRRSWPAFLGGVTASIALLSASALG
CCCHHHHHHHHHCEEEEECCCCCEEHHHHCCCCCCCHHCCHHHHHHHHHHHHHHHHHHHH
LGALLMASERLFSALKLIGALYLFYLAWQSWQAARQAPAAREIESPALAPRFSRLFGRAF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCHHHHHHHHHEE
ILGGSNPKDMLFFAAFLPQFLNAGQPLLPQLLVMTLTWAILDLCCKLAYGLGARGAARYL
EECCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
RTGKGHAWFNRTSAALFGSAGAASLMSR
HCCCCCCHHCCHHHHHHCCCCHHHHHCC
>Mature Secondary Structure 
SLETWLLYTSAALVVILIPGPLSLLMISNSLNYGVRRSWPAFLGGVTASIALLSASALG
CCHHHHHHHHHCEEEEECCCCCEEHHHHCCCCCCCHHCCHHHHHHHHHHHHHHHHHHHH
LGALLMASERLFSALKLIGALYLFYLAWQSWQAARQAPAAREIESPALAPRFSRLFGRAF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCHHHHHHHHHEE
ILGGSNPKDMLFFAAFLPQFLNAGQPLLPQLLVMTLTWAILDLCCKLAYGLGARGAARYL
EECCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
RTGKGHAWFNRTSAALFGSAGAASLMSR
HCCCCCCHHCCHHHHHHCCCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; homoserine [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + homoserine lactone [Cytoplasm] = Proton [Cytoplasm] + homoserine lactone [Periplasm] Proton [Periplasm] + homoserine [Cytoplasm] = Proton [Cytoplasm] + homoserine [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]