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The map label for this gene is guaD [H]

Identifier: 146283413

GI number: 146283413

Start: 3319618

End: 3320925

Strand: Direct

Name: guaD [H]

Synonym: PST_3084

Alternate gene names: 146283413

Gene position: 3319618-3320925 (Clockwise)

Preceding gene: 146283412

Following gene: 146283417

Centisome position: 72.68

GC content: 66.44

Gene sequence:

>1308_bases
ATGCCAAGCACCAAAGCCTACCGTGCCGCCCTGCTCCACTGCCTCGCCGACCCGCGCGAAGTGGGCATCGAGCAAGCCCA
TGAATACTTCGAGGACGGCCTGCTGGTCGTCGAGGACGGCAAGGTCGCGCGCATCGGCCACGCCGCCGAACTGCTGCCGA
CCCTGGCGGCCGGCACCGAGGTTGTCGAATACCCGGACGCCCTGATCACTCCGGGTTTCGTCGACACCCATATCCACTAC
CCGCAGGTCGGCGTCATCGGCTCCTACGGCGCGCAACTGCTGGATTGGCTGGAAACCTACACCTTCCCCAACGAGGGCCG
CTTCAGCGACATGGCCCACGCCCGCCAGCAGGCCGAGCTGTTTCTCGGCGAGCTGCTGCGCAACGGCACCACCACCGCGC
TGGTGTTCGGCACGGTGCACAAGCAGTCGGTGGACGCCTTCTTCGAGGCGTGCGAGAAGCGCAATCTGCGGATGATCGCC
GGCAAAGTGCTGATGGACCGCAACGCCCCGGAGTTCCTCACCGACACCGCCGAGTCCGGCTACGCCGACAGCCGCGAGCT
GATCGAGCGCTGGCACGGCAAGGGCCGTCTGCACTACGCCGTCACCCCGCGTTTCGCCCCGACCAGCACGCCGGAGCAGC
TCACCCTGGCCGGCAAGCTCTTCGCCGAATTCCCCGACCTGTACATGCACACCCACATCTCCGAGAACAAGCAGGAGGTC
GAGTGGGTCAAGGAGCTGTTCCCCGAGCGCAAGGGCTACTTGGACGTCTATGACCACCACGGCCTGATCGGCCCGCGTTC
GGTGTTCGCCCACGGCATCCACCTGTGCGACGACGAGTGCAAACGCCTCGGTGAGACCGGCTCGGCGGTGTCGTTCTGTC
CGACCTCCAACCTGTTCCTCGGCAGCGGCCTGTTCGACCTGGCCAAGGTGGAAGGCTTCGGTGTGCGCGTCGGCCTGGGC
ACCGATGTCGGCGGCGGCACCAGCTTCTCGCAGCTCGCCAGCCTCAACGAGGCGTACAAGGTGTTGCAGCTGCAGGGCCA
GAAGCTCGACGCGTTCAAGGCACTCTATCTCGCCACCCTCGGCGGCGCGCGGGCGCTCTATCTGGACGACCGCATCGGCA
ACCTGCAGCCGGGCAAGGAGGCCGACTTCGTGGTGCTCGACTACAAGGCGACGCCGCTGATCGACTATCGCCTGAGCCAG
GCGACGACCCTGCAGGAAAAGCTCTTCGCCCTGATGATCCTCGGCGACGACCGCGCGGTGAAGGAAACCTTCGCCGCCGG
CGTCTCGGTGCATCGCAAGGCTGGCTAG

Upstream 100 bases:

>100_bases
AGACCAACTGACCATCCGACTTCCTCGTTGCAGGCACGCCCCCGCGGCGGCCTGCCCATGTCAACGCATCACCCTCATAC
AACGATTTTCGAGAGTCACC

Downstream 100 bases:

>100_bases
GGCTGCAACGCCTGCATCAGCGTATCGAGGTTGTGCTCGAACATGCCCAGGTAAGTGCTGGCCGGGCCTGCGCTGGCCAG
CGCATCGGAGTACAGGATGC

Product: guanine deaminase

Products: NA

Alternate protein names: Guanase; Guanine aminase; Guanine aminohydrolase; GAH [H]

Number of amino acids: Translated: 435; Mature: 434

Protein sequence:

>435_residues
MPSTKAYRAALLHCLADPREVGIEQAHEYFEDGLLVVEDGKVARIGHAAELLPTLAAGTEVVEYPDALITPGFVDTHIHY
PQVGVIGSYGAQLLDWLETYTFPNEGRFSDMAHARQQAELFLGELLRNGTTTALVFGTVHKQSVDAFFEACEKRNLRMIA
GKVLMDRNAPEFLTDTAESGYADSRELIERWHGKGRLHYAVTPRFAPTSTPEQLTLAGKLFAEFPDLYMHTHISENKQEV
EWVKELFPERKGYLDVYDHHGLIGPRSVFAHGIHLCDDECKRLGETGSAVSFCPTSNLFLGSGLFDLAKVEGFGVRVGLG
TDVGGGTSFSQLASLNEAYKVLQLQGQKLDAFKALYLATLGGARALYLDDRIGNLQPGKEADFVVLDYKATPLIDYRLSQ
ATTLQEKLFALMILGDDRAVKETFAAGVSVHRKAG

Sequences:

>Translated_435_residues
MPSTKAYRAALLHCLADPREVGIEQAHEYFEDGLLVVEDGKVARIGHAAELLPTLAAGTEVVEYPDALITPGFVDTHIHY
PQVGVIGSYGAQLLDWLETYTFPNEGRFSDMAHARQQAELFLGELLRNGTTTALVFGTVHKQSVDAFFEACEKRNLRMIA
GKVLMDRNAPEFLTDTAESGYADSRELIERWHGKGRLHYAVTPRFAPTSTPEQLTLAGKLFAEFPDLYMHTHISENKQEV
EWVKELFPERKGYLDVYDHHGLIGPRSVFAHGIHLCDDECKRLGETGSAVSFCPTSNLFLGSGLFDLAKVEGFGVRVGLG
TDVGGGTSFSQLASLNEAYKVLQLQGQKLDAFKALYLATLGGARALYLDDRIGNLQPGKEADFVVLDYKATPLIDYRLSQ
ATTLQEKLFALMILGDDRAVKETFAAGVSVHRKAG
>Mature_434_residues
PSTKAYRAALLHCLADPREVGIEQAHEYFEDGLLVVEDGKVARIGHAAELLPTLAAGTEVVEYPDALITPGFVDTHIHYP
QVGVIGSYGAQLLDWLETYTFPNEGRFSDMAHARQQAELFLGELLRNGTTTALVFGTVHKQSVDAFFEACEKRNLRMIAG
KVLMDRNAPEFLTDTAESGYADSRELIERWHGKGRLHYAVTPRFAPTSTPEQLTLAGKLFAEFPDLYMHTHISENKQEVE
WVKELFPERKGYLDVYDHHGLIGPRSVFAHGIHLCDDECKRLGETGSAVSFCPTSNLFLGSGLFDLAKVEGFGVRVGLGT
DVGGGTSFSQLASLNEAYKVLQLQGQKLDAFKALYLATLGGARALYLDDRIGNLQPGKEADFVVLDYKATPLIDYRLSQA
TTLQEKLFALMILGDDRAVKETFAAGVSVHRKAG

Specific function: Catalyzes the hydrolytic deamination of guanine, producing xanthine and ammonia [H]

COG id: COG0402

COG function: function code FR; Cytosine deaminase and related metal-dependent hydrolases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATZ/TRZ family [H]

Homologues:

Organism=Homo sapiens, GI4758426, Length=375, Percent_Identity=37.6, Blast_Score=219, Evalue=4e-57,
Organism=Escherichia coli, GI1789249, Length=434, Percent_Identity=54.8387096774194, Blast_Score=497, Evalue=1e-142,
Organism=Saccharomyces cerevisiae, GI6319963, Length=332, Percent_Identity=36.144578313253, Blast_Score=213, Evalue=4e-56,
Organism=Drosophila melanogaster, GI24643849, Length=433, Percent_Identity=38.568129330254, Blast_Score=236, Evalue=2e-62,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006680
- InterPro:   IPR014311
- InterPro:   IPR011059 [H]

Pfam domain/function: PF01979 Amidohydro_1 [H]

EC number: =3.5.4.3 [H]

Molecular weight: Translated: 47900; Mature: 47769

Theoretical pI: Translated: 5.56; Mature: 5.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSTKAYRAALLHCLADPREVGIEQAHEYFEDGLLVVEDGKVARIGHAAELLPTLAAGTE
CCCCHHHHHHHHHHHCCHHHHCHHHHHHHHHCCEEEEECCCEEECCHHHHHHHHHHCCCH
VVEYPDALITPGFVDTHIHYPQVGVIGSYGAQLLDWLETYTFPNEGRFSDMAHARQQAEL
HHHCCCHHCCCCCEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
FLGELLRNGTTTALVFGTVHKQSVDAFFEACEKRNLRMIAGKVLMDRNAPEFLTDTAESG
HHHHHHHCCCCEEEEEECHHHHHHHHHHHHHHHCCCEEEEHHHHCCCCCCHHHHHCCCCC
YADSRELIERWHGKGRLHYAVTPRFAPTSTPEQLTLAGKLFAEFPDLYMHTHISENKQEV
CCCHHHHHHHHCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHCCHHHEECCCCCCHHHH
EWVKELFPERKGYLDVYDHHGLIGPRSVFAHGIHLCDDECKRLGETGSAVSFCPTSNLFL
HHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHCCCHHHHHCCCCCCEEEECCCCCCEE
GSGLFDLAKVEGFGVRVGLGTDVGGGTSFSQLASLNEAYKVLQLQGQKLDAFKALYLATL
CCCHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHC
GGARALYLDDRIGNLQPGKEADFVVLDYKATPLIDYRLSQATTLQEKLFALMILGDDRAV
CCCEEEEEECCCCCCCCCCCCCEEEEECCCCCEEEEHHHHHHHHHHHHEEEEEECCCHHH
KETFAAGVSVHRKAG
HHHHHHCCCHHCCCC
>Mature Secondary Structure 
PSTKAYRAALLHCLADPREVGIEQAHEYFEDGLLVVEDGKVARIGHAAELLPTLAAGTE
CCCHHHHHHHHHHHCCHHHHCHHHHHHHHHCCEEEEECCCEEECCHHHHHHHHHHCCCH
VVEYPDALITPGFVDTHIHYPQVGVIGSYGAQLLDWLETYTFPNEGRFSDMAHARQQAEL
HHHCCCHHCCCCCEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
FLGELLRNGTTTALVFGTVHKQSVDAFFEACEKRNLRMIAGKVLMDRNAPEFLTDTAESG
HHHHHHHCCCCEEEEEECHHHHHHHHHHHHHHHCCCEEEEHHHHCCCCCCHHHHHCCCCC
YADSRELIERWHGKGRLHYAVTPRFAPTSTPEQLTLAGKLFAEFPDLYMHTHISENKQEV
CCCHHHHHHHHCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHCCHHHEECCCCCCHHHH
EWVKELFPERKGYLDVYDHHGLIGPRSVFAHGIHLCDDECKRLGETGSAVSFCPTSNLFL
HHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHCCCHHHHHCCCCCCEEEECCCCCCEE
GSGLFDLAKVEGFGVRVGLGTDVGGGTSFSQLASLNEAYKVLQLQGQKLDAFKALYLATL
CCCHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHC
GGARALYLDDRIGNLQPGKEADFVVLDYKATPLIDYRLSQATTLQEKLFALMILGDDRAV
CCCEEEEEECCCCCCCCCCCCCEEEEECCCCCEEEEHHHHHHHHHHHHEEEEEECCCHHH
KETFAAGVSVHRKAG
HHHHHHCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503; 10913105 [H]