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The map label for this gene is mltF [H]

Identifier: 146283404

GI number: 146283404

Start: 3305645

End: 3306973

Strand: Direct

Name: mltF [H]

Synonym: PST_3075

Alternate gene names: 146283404

Gene position: 3305645-3306973 (Clockwise)

Preceding gene: 146283401

Following gene: 146283406

Centisome position: 72.37

GC content: 64.41

Gene sequence:

>1329_bases
GTGATCACCCGCAACAGCCCTTCCACCTATTTCCAGGATCGCAATGGCGAAGCCGGCTTCGAGTACGAGCTGGTCAAGCG
TTTCGCCAGCACCCTGGGCGTCGAACTGCAGATCGAAACCGCCGACAACATCGACGACATCTTCAGCCGTCTCAACCGTC
CCGGCGGCCCGGCACTGGCGGCTGCGGGACTGGTTGCCAGCGAAGGCCGCCAGGAACTGGCGCGCTTCACCCGTTCCTAC
CTCGACGTCACCACCCAGGTCGTCTACCACAGCGGCCAGCGCCGACCGACCAGCCCGAAGGATCTGATCGGCAAGCGCAT
CCTGGTGCTCAAGGGCAGCAGCCAGGCGGAGAAGCTGGCCTCGCTGCAGGTGGAGTACCCCGAGCTGCGCTACGACGAAT
CCGACGCGGTGGAAGTCGTCGACCTGCTGCGCATGGTCGATGAAGGCCAGATCGACCTGACCCTGGTGGAATCCAACGAG
ATGTCCATGAACCAGGTGTATTTCAGCAACATCCGCGCCGGCTTCGACGTCGGCGAACAGAACAGCCTGGCCTGGGTGGT
CGCCAAAGGCGAGGATGACAGCCTGCTCAAGGCGGCCGACGCCTTCCTCGAGCAGGCCCAGCAGAACGGTACGTTGCAGC
GCCTGCGCGAGCGCTACTACGGCCATGTCGACGTGCTCGGCTACGTCGGCGCCTATGCCTTCGCCAAGCACCTGCAGCAG
CGTCTGCCACGTTATGAAAAGGCCTTCCGTGAAACCGCCAAGGAACATGGCATCGACTGGCGCCTGCTGGCTGCGATCGG
CTACCAGGAGTCCCACTGGCAGCCGGAAGCCACCTCCAAGACCGGCGTGCGCGGACTGATGATGCTGACCCTGCGCACCG
CCAATGCCATGGGCGTGACCAACCGTCTCGATCCGGTACAGAGCATTCAGGGTGGCGGCAAGTACCTGGTGCAGGTGCAT
GCCAGCCTGCCCGAGAGTATCGAGGAGCCGGACCGCACCTGGTTCGCCCTGGCCGCCTACAACGTCGGCGGAGGCCATCT
GGAAGATGCACGCAAGCTGGCCGAGGCCGAAGGGCTGGACCCCAACAAGTGGCTGGACGTGAAGCAGATGCTGCCGCGCC
TGTCGCAGAAGCAGTGGTACAGCAAGACCCGCTACGGCTATGCCCGCGGCGGTGAGCCGGTGCACTTCGTGGCCAACATC
CGCCGCTACTACGACATCCTCACCTGGGTGACCCAGCCGCAGATGGAGGGTCAGCAGCTGGCCAAGAGCGAGCTGCACAT
CCCGGGCATCAACTCCACCGACCTCATGGAAGAGCTGCCGCCGCTCTGA

Upstream 100 bases:

>100_bases
CGCCATCTGGCTGTTGGCGATCGGAATCTTCCTGATGCTCGGCAGCTGCGCCGAAAAGCCCAGCGAGCTCGAGCGCATTA
AGGAGGAAGGCGTACTGCGC

Downstream 100 bases:

>100_bases
CCCCTACGGCGCCGTTTCACCTCAGGACGGGCCGACGCCCATGCGCCGCAGCAGCGCCTTCTCGATCTCCAGGATCAGCA
GCACCGCCACGCCGGCCAGT

Product: putative transglycosylase

Products: NA

Alternate protein names: Murein lyase F [H]

Number of amino acids: Translated: 442; Mature: 442

Protein sequence:

>442_residues
MITRNSPSTYFQDRNGEAGFEYELVKRFASTLGVELQIETADNIDDIFSRLNRPGGPALAAAGLVASEGRQELARFTRSY
LDVTTQVVYHSGQRRPTSPKDLIGKRILVLKGSSQAEKLASLQVEYPELRYDESDAVEVVDLLRMVDEGQIDLTLVESNE
MSMNQVYFSNIRAGFDVGEQNSLAWVVAKGEDDSLLKAADAFLEQAQQNGTLQRLRERYYGHVDVLGYVGAYAFAKHLQQ
RLPRYEKAFRETAKEHGIDWRLLAAIGYQESHWQPEATSKTGVRGLMMLTLRTANAMGVTNRLDPVQSIQGGGKYLVQVH
ASLPESIEEPDRTWFALAAYNVGGGHLEDARKLAEAEGLDPNKWLDVKQMLPRLSQKQWYSKTRYGYARGGEPVHFVANI
RRYYDILTWVTQPQMEGQQLAKSELHIPGINSTDLMEELPPL

Sequences:

>Translated_442_residues
MITRNSPSTYFQDRNGEAGFEYELVKRFASTLGVELQIETADNIDDIFSRLNRPGGPALAAAGLVASEGRQELARFTRSY
LDVTTQVVYHSGQRRPTSPKDLIGKRILVLKGSSQAEKLASLQVEYPELRYDESDAVEVVDLLRMVDEGQIDLTLVESNE
MSMNQVYFSNIRAGFDVGEQNSLAWVVAKGEDDSLLKAADAFLEQAQQNGTLQRLRERYYGHVDVLGYVGAYAFAKHLQQ
RLPRYEKAFRETAKEHGIDWRLLAAIGYQESHWQPEATSKTGVRGLMMLTLRTANAMGVTNRLDPVQSIQGGGKYLVQVH
ASLPESIEEPDRTWFALAAYNVGGGHLEDARKLAEAEGLDPNKWLDVKQMLPRLSQKQWYSKTRYGYARGGEPVHFVANI
RRYYDILTWVTQPQMEGQQLAKSELHIPGINSTDLMEELPPL
>Mature_442_residues
MITRNSPSTYFQDRNGEAGFEYELVKRFASTLGVELQIETADNIDDIFSRLNRPGGPALAAAGLVASEGRQELARFTRSY
LDVTTQVVYHSGQRRPTSPKDLIGKRILVLKGSSQAEKLASLQVEYPELRYDESDAVEVVDLLRMVDEGQIDLTLVESNE
MSMNQVYFSNIRAGFDVGEQNSLAWVVAKGEDDSLLKAADAFLEQAQQNGTLQRLRERYYGHVDVLGYVGAYAFAKHLQQ
RLPRYEKAFRETAKEHGIDWRLLAAIGYQESHWQPEATSKTGVRGLMMLTLRTANAMGVTNRLDPVQSIQGGGKYLVQVH
ASLPESIEEPDRTWFALAAYNVGGGHLEDARKLAEAEGLDPNKWLDVKQMLPRLSQKQWYSKTRYGYARGGEPVHFVANI
RRYYDILTWVTQPQMEGQQLAKSELHIPGINSTDLMEELPPL

Specific function: Murein-degrading enzyme that degrades murein glycan strands and insoluble, high-molecular weight murein sacculi, with the concomitant formation of a 1,6-anhydromuramoyl product. Lytic transglycosylases (LTs) play an integral role in the metabolism of the

COG id: COG4623

COG function: function code M; Predicted soluble lytic transglycosylase fused to an ABC-type amino acid-binding protein

Gene ontology:

Cell location: Cell outer membrane; Peripheral membrane protein. Note=Attached to the inner leaflet of the outer membrane [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transglycosylase slt family [H]

Homologues:

Organism=Escherichia coli, GI171474010, Length=444, Percent_Identity=38.5135135135135, Blast_Score=296, Evalue=1e-81,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008258
- InterPro:   IPR001638
- InterPro:   IPR000189 [H]

Pfam domain/function: PF00497 SBP_bac_3; PF01464 SLT [H]

EC number: NA

Molecular weight: Translated: 49748; Mature: 49748

Theoretical pI: Translated: 5.52; Mature: 5.52

Prosite motif: PS00922 TRANSGLYCOSYLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITRNSPSTYFQDRNGEAGFEYELVKRFASTLGVELQIETADNIDDIFSRLNRPGGPALA
CCCCCCCCCCEECCCCCCCCHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHCCCCCCHHH
AAGLVASEGRQELARFTRSYLDVTTQVVYHSGQRRPTSPKDLIGKRILVLKGSSQAEKLA
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCEEEEEECCCHHHHHH
SLQVEYPELRYDESDAVEVVDLLRMVDEGQIDLTLVESNEMSMNQVYFSNIRAGFDVGEQ
HHEECCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHCCCCCCCC
NSLAWVVAKGEDDSLLKAADAFLEQAQQNGTLQRLRERYYGHVDVLGYVGAYAFAKHLQQ
CCEEEEEEECCCCHHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
RLPRYEKAFRETAKEHGIDWRLLAAIGYQESHWQPEATSKTGVRGLMMLTLRTANAMGVT
HCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCHHHEEEEEEHHCCCCCCC
NRLDPVQSIQGGGKYLVQVHASLPESIEEPDRTWFALAAYNVGGGHLEDARKLAEAEGLD
CCCCHHHHHCCCCEEEEEEEHHCCHHHCCCCCCEEEEEEEECCCCCHHHHHHHHHHCCCC
PNKWLDVKQMLPRLSQKQWYSKTRYGYARGGEPVHFVANIRRYYDILTWVTQPQMEGQQL
CHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHH
AKSELHIPGINSTDLMEELPPL
HHHCCCCCCCCCHHHHHHCCCC
>Mature Secondary Structure
MITRNSPSTYFQDRNGEAGFEYELVKRFASTLGVELQIETADNIDDIFSRLNRPGGPALA
CCCCCCCCCCEECCCCCCCCHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHCCCCCCHHH
AAGLVASEGRQELARFTRSYLDVTTQVVYHSGQRRPTSPKDLIGKRILVLKGSSQAEKLA
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCEEEEEECCCHHHHHH
SLQVEYPELRYDESDAVEVVDLLRMVDEGQIDLTLVESNEMSMNQVYFSNIRAGFDVGEQ
HHEECCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHCCCCCCCC
NSLAWVVAKGEDDSLLKAADAFLEQAQQNGTLQRLRERYYGHVDVLGYVGAYAFAKHLQQ
CCEEEEEEECCCCHHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
RLPRYEKAFRETAKEHGIDWRLLAAIGYQESHWQPEATSKTGVRGLMMLTLRTANAMGVT
HCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCHHHEEEEEEHHCCCCCCC
NRLDPVQSIQGGGKYLVQVHASLPESIEEPDRTWFALAAYNVGGGHLEDARKLAEAEGLD
CCCCHHHHHCCCCEEEEEEEHHCCHHHCCCCCCEEEEEEEECCCCCHHHHHHHHHHCCCC
PNKWLDVKQMLPRLSQKQWYSKTRYGYARGGEPVHFVANIRRYYDILTWVTQPQMEGQQL
CHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHH
AKSELHIPGINSTDLMEELPPL
HHHCCCCCCCCCHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA