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The map label for this gene is tapB [H]

Identifier: 146283383

GI number: 146283383

Start: 3283461

End: 3283673

Strand: Direct

Name: tapB [H]

Synonym: PST_3054

Alternate gene names: 146283383

Gene position: 3283461-3283673 (Clockwise)

Preceding gene: 146283376

Following gene: 146283384

Centisome position: 71.89

GC content: 53.99

Gene sequence:

>213_bases
ATGTCGAATCGTCAGAACGGTACCGTCAAGTGGTTTAACGACGAGAAAGGTTTTGGTTTCATCACTCCCGAGAGCGGTCC
GGATCTGTTCGTGCACTTCCGCGCGATCGAAGGCAACGGCTTCAAGAGCCTGAAAGAAGGCCAGAAAGTCAGCTTCATCG
CCGTGCAAGGTCAAAAGGGCATGCAGGCCGACCAGGTTCAGATCCTGGGCTAA

Upstream 100 bases:

>100_bases
ATCCGCTACTCCGCTCGACTCTCTTACTCCTTGCAACCAGTTCCAGCCTCCTGTTTTTGCGGGAAGCAATTCTACTTTTG
AGTTTCAAGGATACAAAGAC

Downstream 100 bases:

>100_bases
GCCTTCTCTCCGAAAAGCCCCTGATGGCAACATCAGGGGCTTTTTTATGCGCGCAATTCCGTAGAATGCCCCGCCTTCCA
CTTGATCGAGAGACGCAATG

Product: cold acclimation protein B

Products: NA

Alternate protein names: E8.0 [H]

Number of amino acids: Translated: 70; Mature: 69

Protein sequence:

>70_residues
MSNRQNGTVKWFNDEKGFGFITPESGPDLFVHFRAIEGNGFKSLKEGQKVSFIAVQGQKGMQADQVQILG

Sequences:

>Translated_70_residues
MSNRQNGTVKWFNDEKGFGFITPESGPDLFVHFRAIEGNGFKSLKEGQKVSFIAVQGQKGMQADQVQILG
>Mature_69_residues
SNRQNGTVKWFNDEKGFGFITPESGPDLFVHFRAIEGNGFKSLKEGQKVSFIAVQGQKGMQADQVQILG

Specific function: Affects cell viability at low temperatures [H]

COG id: COG1278

COG function: function code K; Cold shock proteins

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CSD (cold-shock) domain [H]

Homologues:

Organism=Escherichia coli, GI1789979, Length=70, Percent_Identity=58.5714285714286, Blast_Score=81, Evalue=2e-17,
Organism=Escherichia coli, GI2367114, Length=70, Percent_Identity=57.1428571428571, Blast_Score=79, Evalue=4e-17,
Organism=Escherichia coli, GI1786841, Length=64, Percent_Identity=60.9375, Blast_Score=76, Evalue=3e-16,
Organism=Escherichia coli, GI1787834, Length=70, Percent_Identity=57.1428571428571, Blast_Score=75, Evalue=6e-16,
Organism=Escherichia coli, GI1788126, Length=64, Percent_Identity=60.9375, Blast_Score=74, Evalue=1e-15,
Organism=Escherichia coli, GI1787839, Length=69, Percent_Identity=53.6231884057971, Blast_Score=74, Evalue=2e-15,
Organism=Escherichia coli, GI1787107, Length=63, Percent_Identity=50.7936507936508, Blast_Score=68, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019844
- InterPro:   IPR012156
- InterPro:   IPR011129
- InterPro:   IPR002059
- InterPro:   IPR012340
- InterPro:   IPR016027 [H]

Pfam domain/function: PF00313 CSD [H]

EC number: NA

Molecular weight: Translated: 7747; Mature: 7616

Theoretical pI: Translated: 9.28; Mature: 9.28

Prosite motif: PS00352 COLD_SHOCK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNRQNGTVKWFNDEKGFGFITPESGPDLFVHFRAIEGNGFKSLKEGQKVSFIAVQGQKG
CCCCCCCCEEEEECCCCCEEECCCCCCCEEEEEEEECCCCCCCCCCCCEEEEEEEECCCC
MQADQVQILG
CCCCEEEEEC
>Mature Secondary Structure 
SNRQNGTVKWFNDEKGFGFITPESGPDLFVHFRAIEGNGFKSLKEGQKVSFIAVQGQKG
CCCCCCCEEEEECCCCCEEECCCCCCCEEEEEEEECCCCCCCCCCCCEEEEEEEECCCC
MQADQVQILG
CCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9393697 [H]