Click here to switch to the map view.

The map label for this gene is usf [H]

Identifier: 146283322

GI number: 146283322

Start: 3219808

End: 3220614

Strand: Direct

Name: usf [H]

Synonym: PST_2987

Alternate gene names: 146283322

Gene position: 3219808-3220614 (Clockwise)

Preceding gene: 146283321

Following gene: 146283323

Centisome position: 70.5

GC content: 65.68

Gene sequence:

>807_bases
ATGCGCCATTGCCTGCTCGGCCTGCTCATGGCTGCCAGCGTCACCGCCAATGCCGCGGTACAGACGCAGGAAATCCCCTA
CACCGCTGCCGACGGCACCAAGATGATCGGTTACTACGCCTACGACGATGCCATCGAGGGCAAGCGCCCCGGCATCGTGG
TGGTGCATGAATGGTGGGGCCTTAACGACTACGCCAAGCAACGCGCCCGCGACCTCGCCGAGCTGGGCTACAGCGCCCTT
GCCATCGACATGTATGGCGAGGGCAGAAACACCGAGCACCCCAAGGACGCCATGAGCTTCATGCAGGCCGCACTGAAGGA
CGCCGATGCGGCCAAGGGCCGCTTCAACGCCGGCCTGGATCTGCTCAAGGAGCAGGCACAGACGGATACCGACAAGCTGG
GCGCGGTCGGCTATTGCTTTGGCGGCAAGGTAGTCCTGGACATGGCGCGCCAGGGCGTGCCGCTGGACGGCGTGGTCAGC
TTCCACGGCGCATTGGCCACCGAAACCCGCGCCGCGCCGGGTAGCGTCAAGGCGCGAGTGCTGGTCGAGCACGGCGCCGA
AGACAGCATGATCAGCGCCGACGATGTCGCGGCCCTGAACGTCGAGATGGTCAAGGCCGGCGCCGATTACCAGTTCGTCA
GCCTGCCGGGGGCCAAGCACGGTTTCACCAATCCGGGCGCCGATGCCCATCAGAAGAACGGCCTCGACGTGGCCTATCAA
AAAGCCGCCGACGAGCGCTCCTGGCGAGATATGCAGCGGTTCTTCGAGGACACCTTCGGCCCGTCGACGCCTGCCCGGGC
GCAGTGA

Upstream 100 bases:

>100_bases
ATGCCGCCAGACGAACCCTCACGCTGCGCGTACGGTCGAACAGCCGCTATGCTTGCTCGAATGCCTCTATCCCACTAATG
CCAGTCAAGGAGACCTCACG

Downstream 100 bases:

>100_bases
CCGTTCGACGCGCCGCGTTCGATTCGCCCCGGCGGCACGCCTGCCGCCGGGGCTCGTGCTAGCATTGCGCGCACAACTCA
GCGTCGAACACGAGTCATGG

Product: dienelactone hydrolase family protein

Products: oxohex-2-enedioate [C]

Alternate protein names: NA

Number of amino acids: Translated: 268; Mature: 268

Protein sequence:

>268_residues
MRHCLLGLLMAASVTANAAVQTQEIPYTAADGTKMIGYYAYDDAIEGKRPGIVVVHEWWGLNDYAKQRARDLAELGYSAL
AIDMYGEGRNTEHPKDAMSFMQAALKDADAAKGRFNAGLDLLKEQAQTDTDKLGAVGYCFGGKVVLDMARQGVPLDGVVS
FHGALATETRAAPGSVKARVLVEHGAEDSMISADDVAALNVEMVKAGADYQFVSLPGAKHGFTNPGADAHQKNGLDVAYQ
KAADERSWRDMQRFFEDTFGPSTPARAQ

Sequences:

>Translated_268_residues
MRHCLLGLLMAASVTANAAVQTQEIPYTAADGTKMIGYYAYDDAIEGKRPGIVVVHEWWGLNDYAKQRARDLAELGYSAL
AIDMYGEGRNTEHPKDAMSFMQAALKDADAAKGRFNAGLDLLKEQAQTDTDKLGAVGYCFGGKVVLDMARQGVPLDGVVS
FHGALATETRAAPGSVKARVLVEHGAEDSMISADDVAALNVEMVKAGADYQFVSLPGAKHGFTNPGADAHQKNGLDVAYQ
KAADERSWRDMQRFFEDTFGPSTPARAQ
>Mature_268_residues
MRHCLLGLLMAASVTANAAVQTQEIPYTAADGTKMIGYYAYDDAIEGKRPGIVVVHEWWGLNDYAKQRARDLAELGYSAL
AIDMYGEGRNTEHPKDAMSFMQAALKDADAAKGRFNAGLDLLKEQAQTDTDKLGAVGYCFGGKVVLDMARQGVPLDGVVS
FHGALATETRAAPGSVKARVLVEHGAEDSMISADDVAALNVEMVKAGADYQFVSLPGAKHGFTNPGADAHQKNGLDVAYQ
KAADERSWRDMQRFFEDTFGPSTPARAQ

Specific function: Unknown

COG id: COG0412

COG function: function code Q; Dienelactone hydrolase and related enzymes

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI48994982, Length=267, Percent_Identity=24.3445692883895, Blast_Score=66, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17532655, Length=243, Percent_Identity=34.156378600823, Blast_Score=133, Evalue=9e-32,
Organism=Caenorhabditis elegans, GI17536419, Length=242, Percent_Identity=31.404958677686, Blast_Score=120, Evalue=6e-28,
Organism=Caenorhabditis elegans, GI17532653, Length=243, Percent_Identity=32.9218106995885, Blast_Score=108, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI193204305, Length=214, Percent_Identity=34.5794392523364, Blast_Score=106, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17536415, Length=241, Percent_Identity=26.5560165975104, Blast_Score=95, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI17534829, Length=151, Percent_Identity=35.0993377483444, Blast_Score=79, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002925 [H]

Pfam domain/function: PF01738 DLH [H]

EC number: 3.1.1.45 [C]

Molecular weight: Translated: 28822; Mature: 28822

Theoretical pI: Translated: 5.15; Mature: 5.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRHCLLGLLMAASVTANAAVQTQEIPYTAADGTKMIGYYAYDDAIEGKRPGIVVVHEWWG
CHHHHHHHHHHHHHHHCCEEEECCCCEEECCCCEEEEEEEECCCCCCCCCCEEEEECCCC
LNDYAKQRARDLAELGYSALAIDMYGEGRNTEHPKDAMSFMQAALKDADAAKGRFNAGLD
CCHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHCCHHCCCCCHHHHH
LLKEQAQTDTDKLGAVGYCFGGKVVLDMARQGVPLDGVVSFHGALATETRAAPGSVKARV
HHHHHHHCCHHHHCCEEEECCCHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCEEEE
LVEHGAEDSMISADDVAALNVEMVKAGADYQFVSLPGAKHGFTNPGADAHQKNGLDVAYQ
EEECCCCCCCCCCCCHHHHHHEEEECCCCEEEEECCCCCCCCCCCCCCCHHCCCCCHHHH
KAADERSWRDMQRFFEDTFGPSTPARAQ
HHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MRHCLLGLLMAASVTANAAVQTQEIPYTAADGTKMIGYYAYDDAIEGKRPGIVVVHEWWG
CHHHHHHHHHHHHHHHCCEEEECCCCEEECCCCEEEEEEEECCCCCCCCCCEEEEECCCC
LNDYAKQRARDLAELGYSALAIDMYGEGRNTEHPKDAMSFMQAALKDADAAKGRFNAGLD
CCHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHCCHHCCCCCHHHHH
LLKEQAQTDTDKLGAVGYCFGGKVVLDMARQGVPLDGVVSFHGALATETRAAPGSVKARV
HHHHHHHCCHHHHCCEEEECCCHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCEEEE
LVEHGAEDSMISADDVAALNVEMVKAGADYQFVSLPGAKHGFTNPGADAHQKNGLDVAYQ
EEECCCCCCCCCCCCHHHHHHEEEECCCCEEEEECCCCCCCCCCCCCCCHHCCCCCHHHH
KAADERSWRDMQRFFEDTFGPSTPARAQ
HHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 4-carboxymethylenebut-2-en-4-olide; H2O [C]

Specific reaction: 4-carboxymethylenebut-2-en-4-olide + H2O = 4 oxohex-2-enedioate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7592443 [H]