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The map label for this gene is cspA [H]

Identifier: 146283150

GI number: 146283150

Start: 3037821

End: 3038552

Strand: Direct

Name: cspA [H]

Synonym: PST_2815

Alternate gene names: 146283150

Gene position: 3037821-3038552 (Clockwise)

Preceding gene: 146283149

Following gene: 146283153

Centisome position: 66.51

GC content: 60.93

Gene sequence:

>732_bases
TTGCCCAGACGCGCGCGGTTCCTCGAACAGTGTTCAGCCCTGCCCGATGAACGTTTTCCAGCGAGCCTTGGCATTGCCGC
CTCTCTGTCGTGTCATCAAATCGTGAGTCATACAAGAATGTTAAAGATCGTCCATGTTCTAGCGGGCGTCATCGCTCTGC
TGCTGTCCTTCATTCCCAGCCTGCGCGGCGCTGAGCCGCTGTTACTGCAACCCGAAGCCCTGTGCTTGCTGATGCTCGGC
CTGCTCAATGTTCAATTCGCGCCGTCGGCACTACTGAATGACAGCCGGACCCGCCCGGTGATCATCGCCGCGTCGGCCCT
CCTGCTCCTGTCCATCGCCCTGCAAGCCGTCTTCGTACTCGCCTCACTGCCACAAATCGCCGGTCAACCCGCCACCCTGG
CCAGCCTGCTGCTCGCCTTTGTCGCAGTGCTGCTGCACCTGGCAACACCTCAGCGCCGCAACAAGCAACCCAAACCGTCT
CGCATGAGTACGAGCGCGAGTGTTCCCTCCGCGGCCGGACGCGAAGCCGGCACCGTCAAGTGGTTCAATACCTCAAAGGG
GTTCGGTTTCATTTCTCGGGACAGCGGTGACGATGTATTCGTCCACTTCCGCGCCATTCGTGGCGAGGGGCACCGCATCC
TTGTGGAAGGCCAGCGAGTGGAGTTCACCATCATGATGCGCGACAAGGGTCTGCAGGCTGAAGACGTCGTCCCGGTCGAA
GCTGGCCGCTAA

Upstream 100 bases:

>100_bases
CTGCGCGCCCTGTCCCGCTTCGGCCCTCCTGCGCTCGGATCCCGCCGCTTCCACGCTCATTTGAGCGCCCTACGCCTTTC
CTGTTAAATACGCCGCGCTA

Downstream 100 bases:

>100_bases
CGCAGTGCCTGGCGGGGCCAGCGCCCCGCCAGCGGTTCTTCAATAATGAGGTGGCGGCGCCTCGTCCTCATCGGCAGCAC
CCAGCGAACCCTGCATCTCC

Product: cold-shock DNA-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 243; Mature: 242

Protein sequence:

>243_residues
MPRRARFLEQCSALPDERFPASLGIAASLSCHQIVSHTRMLKIVHVLAGVIALLLSFIPSLRGAEPLLLQPEALCLLMLG
LLNVQFAPSALLNDSRTRPVIIAASALLLLSIALQAVFVLASLPQIAGQPATLASLLLAFVAVLLHLATPQRRNKQPKPS
RMSTSASVPSAAGREAGTVKWFNTSKGFGFISRDSGDDVFVHFRAIRGEGHRILVEGQRVEFTIMMRDKGLQAEDVVPVE
AGR

Sequences:

>Translated_243_residues
MPRRARFLEQCSALPDERFPASLGIAASLSCHQIVSHTRMLKIVHVLAGVIALLLSFIPSLRGAEPLLLQPEALCLLMLG
LLNVQFAPSALLNDSRTRPVIIAASALLLLSIALQAVFVLASLPQIAGQPATLASLLLAFVAVLLHLATPQRRNKQPKPS
RMSTSASVPSAAGREAGTVKWFNTSKGFGFISRDSGDDVFVHFRAIRGEGHRILVEGQRVEFTIMMRDKGLQAEDVVPVE
AGR
>Mature_242_residues
PRRARFLEQCSALPDERFPASLGIAASLSCHQIVSHTRMLKIVHVLAGVIALLLSFIPSLRGAEPLLLQPEALCLLMLGL
LNVQFAPSALLNDSRTRPVIIAASALLLLSIALQAVFVLASLPQIAGQPATLASLLLAFVAVLLHLATPQRRNKQPKPSR
MSTSASVPSAAGREAGTVKWFNTSKGFGFISRDSGDDVFVHFRAIRGEGHRILVEGQRVEFTIMMRDKGLQAEDVVPVEA
GR

Specific function: Binds To And Stimulates The Transcription Of The CCAAT- Containing, Cold-Shock-Inducible Promoters Of The H-Ns And Gyra Proteins. Binds Also To The Inverted Repeat 5'-ATTGG-3'. [C]

COG id: COG1278

COG function: function code K; Cold shock proteins

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CSD (cold-shock) domain [H]

Homologues:

Organism=Escherichia coli, GI1789979, Length=66, Percent_Identity=54.5454545454545, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1786841, Length=62, Percent_Identity=58.0645161290323, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1788126, Length=64, Percent_Identity=56.25, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1787107, Length=67, Percent_Identity=49.2537313432836, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1787839, Length=67, Percent_Identity=50.7462686567164, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI2367114, Length=67, Percent_Identity=50.7462686567164, Blast_Score=68, Evalue=4e-13,
Organism=Escherichia coli, GI1787834, Length=67, Percent_Identity=49.2537313432836, Blast_Score=63, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019844
- InterPro:   IPR012156
- InterPro:   IPR011129
- InterPro:   IPR002059
- InterPro:   IPR012340
- InterPro:   IPR016027 [H]

Pfam domain/function: PF00313 CSD [H]

EC number: NA

Molecular weight: Translated: 26213; Mature: 26082

Theoretical pI: Translated: 10.95; Mature: 10.95

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS00352 COLD_SHOCK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRRARFLEQCSALPDERFPASLGIAASLSCHQIVSHTRMLKIVHVLAGVIALLLSFIPS
CCHHHHHHHHHHCCCCCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LRGAEPLLLQPEALCLLMLGLLNVQFAPSALLNDSRTRPVIIAASALLLLSIALQAVFVL
HCCCCCEEECHHHHHHHHHHHHCCCCCCHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHHH
ASLPQIAGQPATLASLLLAFVAVLLHLATPQRRNKQPKPSRMSTSASVPSAAGREAGTVK
HHHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCEEE
WFNTSKGFGFISRDSGDDVFVHFRAIRGEGHRILVEGQRVEFTIMMRDKGLQAEDVVPVE
EEECCCCCEEEECCCCCEEEEEEEEECCCCCEEEEECCEEEEEEEEECCCCCCCCCCCCC
AGR
CCC
>Mature Secondary Structure 
PRRARFLEQCSALPDERFPASLGIAASLSCHQIVSHTRMLKIVHVLAGVIALLLSFIPS
CHHHHHHHHHHCCCCCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LRGAEPLLLQPEALCLLMLGLLNVQFAPSALLNDSRTRPVIIAASALLLLSIALQAVFVL
HCCCCCEEECHHHHHHHHHHHHCCCCCCHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHHH
ASLPQIAGQPATLASLLLAFVAVLLHLATPQRRNKQPKPSRMSTSASVPSAAGREAGTVK
HHHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCEEE
WFNTSKGFGFISRDSGDDVFVHFRAIRGEGHRILVEGQRVEFTIMMRDKGLQAEDVVPVE
EEECCCCCEEEECCCCCEEEEEEEEECCCCCEEEEECCEEEEEEEEECCCCCCCCCCCCC
AGR
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]