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The map label for this gene is lig [H]

Identifier: 146283001

GI number: 146283001

Start: 2883507

End: 2885213

Strand: Direct

Name: lig [H]

Synonym: PST_2662

Alternate gene names: 146283001

Gene position: 2883507-2885213 (Clockwise)

Preceding gene: 146283000

Following gene: 146283002

Centisome position: 63.13

GC content: 67.96

Gene sequence:

>1707_bases
ATGAAAGCCTTCGCCACGCTCTACAGTCGCCTGGACGCCACCACGTCGAGCAATGCCAAACTGGCCGCGATGCGCGACTA
CTTTCGCGAGGCCGATCCGTCCGACGCTGCCTGGGCGGTCTACTTCCTGGCCGGCGGTCGCCCGCGTCAACTGGTCCCGA
CCCGCGCCCTGCGCGAAACCGCCATGCAGGCGGCGGGCCTGCCCGAATGGCTGTTCGAGGAAAGCTACCAGGCCGTTGGC
GACATGGCCGAGACCATCTCGCTGCTGATGTCCGAGGCCGAACACAGCTCCGAGGACGGGCTGGCCGTGTGGATGCAGGA
CCGACTGCTACCGCTGCGCGGCCTGCCGCCGGAGGAGCTGGCCGAACGCTTGCCGGCGCTCTGGGCACAGCTGGATCGGC
TCAGCCTGATGGTCTGCATCAAGCTGATCACCGGCGCCTTTCGCGTCGGCGTGTCGAAGCTGCTAGTCACTCGCGCGCTG
GCCTCGCTGGCCGACCTCGACCCCAAGCGCGTCGCGCAGCGCCTGGTCGGCTACACCGACCTGTCCCATCGGCCCAGCGC
CGAGGGCTATCGCGCGCTGATCGCCGAAGAATCCGAGCATGAGCATGCCCAGCGTGGTGGCCAGCCCTACCCGTTCTTTC
TCGCTCATGCGCTGCAGGCCCCGGTGGACACGTTCGACACGCTGCTTGGCGCGCCGGACAACTGGTTCATCGAATGGAAG
TGGGATGGCATCCGCGCCCAGCTGGTCAAGCGCGACGGGCAGATCTGGGTCTGGTCGCGCGGTGAGGAGCTGGTCAGCGA
GCGCTTTCCCGAGCTGTGCGAGCTGGCCCGCTGCCTGCCCGACGGCACGGTGATCGATGGCGAGATCCTGGTCTGGAAGC
ATGACCCGGAGACTCCGGCCGGCGAGCTGTTCGACGCTGCCAGCACCGGCGAGCCCAGCGCCGATGGCTTCGGCGTGCAG
CCCTTCGCCCTGCTGCAACAGCGCATCGGCCGCAAGAACCTGACCGCCAAGGTCCTGCAGGATGCGCCGGTCGCAGTGCT
CGCCTACGACCTGCTGGAATGGCAGGGCGACGACTGGCGGCAGCGCGAGCACCGCGAACGCCGCCAGCAGCTCGAGGCGG
TCGTGGGCCAATGCCCGAGCCCGCAGCTGATGCTTTCACCACTCGTAAGCGGAGCGGATTGGCAGGATCTGGCCCGCCAG
CGCGAAGCCTCACGCGCGCTGGGCGTGGAAGGCATGATGATCAAGGCGCGCGCGGCCCAGTACGGCGTCGGTCGGACCAA
GGACGTCGGCGTCTGGTGGAAATGGAAGATCGACCCCTACTCCGTCGATGCCGTGCTGATCTATGCCCAGCGCGGTCATG
GCCGCCGCGCCAGCCTCTACACCGACTACACCTTTGCCGTCTGGGACGGCGAGCCGGGCGACCACGAGCGCAAACTCGTG
CCCTTCGCCAAGGCCTATTCCGGCCTCACCGACGAGGAAATGCGCAAGGTGGACGCCATCGTGCGCAAGACCACCGTGGA
GAAGTTCGGCCCGGTGCGCAGCGTCACGCCGACCCTGGTGTTCGAACTCGGCTTCGAAGGCATCGCCGCCAGCAGCCGCC
ACAAGAGCGGCATCGCCGTGCGCTTCCCGCGCATGCTGCGCTGGCGCCTCGACAAGCCGGTGGAGGAAGCCGATACCCTG
GCCACCCTGCGGGAATTGCTGGGATGA

Upstream 100 bases:

>100_bases
GAATACGGCGAAGAGGACGATGCCGCGACGGAGGGCACCGCATGATCGAAGGCCACGCCGCTTTCCCGTGGCGCGGCGAC
CGCCGAAGCGGAGGCGCCGC

Downstream 100 bases:

>100_bases
AACCAAAGCACCCGGCAGCCGCTCGGCGCTCATCACAACCCCCTCCCAAGAGCCGAGCCGAGCAGCCACACGATCACGAC
CCACTCCCTCAAGAAGAGGG

Product: ATP-dependent DNA ligase

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [ATP] [H]

Number of amino acids: Translated: 568; Mature: 568

Protein sequence:

>568_residues
MKAFATLYSRLDATTSSNAKLAAMRDYFREADPSDAAWAVYFLAGGRPRQLVPTRALRETAMQAAGLPEWLFEESYQAVG
DMAETISLLMSEAEHSSEDGLAVWMQDRLLPLRGLPPEELAERLPALWAQLDRLSLMVCIKLITGAFRVGVSKLLVTRAL
ASLADLDPKRVAQRLVGYTDLSHRPSAEGYRALIAEESEHEHAQRGGQPYPFFLAHALQAPVDTFDTLLGAPDNWFIEWK
WDGIRAQLVKRDGQIWVWSRGEELVSERFPELCELARCLPDGTVIDGEILVWKHDPETPAGELFDAASTGEPSADGFGVQ
PFALLQQRIGRKNLTAKVLQDAPVAVLAYDLLEWQGDDWRQREHRERRQQLEAVVGQCPSPQLMLSPLVSGADWQDLARQ
REASRALGVEGMMIKARAAQYGVGRTKDVGVWWKWKIDPYSVDAVLIYAQRGHGRRASLYTDYTFAVWDGEPGDHERKLV
PFAKAYSGLTDEEMRKVDAIVRKTTVEKFGPVRSVTPTLVFELGFEGIAASSRHKSGIAVRFPRMLRWRLDKPVEEADTL
ATLRELLG

Sequences:

>Translated_568_residues
MKAFATLYSRLDATTSSNAKLAAMRDYFREADPSDAAWAVYFLAGGRPRQLVPTRALRETAMQAAGLPEWLFEESYQAVG
DMAETISLLMSEAEHSSEDGLAVWMQDRLLPLRGLPPEELAERLPALWAQLDRLSLMVCIKLITGAFRVGVSKLLVTRAL
ASLADLDPKRVAQRLVGYTDLSHRPSAEGYRALIAEESEHEHAQRGGQPYPFFLAHALQAPVDTFDTLLGAPDNWFIEWK
WDGIRAQLVKRDGQIWVWSRGEELVSERFPELCELARCLPDGTVIDGEILVWKHDPETPAGELFDAASTGEPSADGFGVQ
PFALLQQRIGRKNLTAKVLQDAPVAVLAYDLLEWQGDDWRQREHRERRQQLEAVVGQCPSPQLMLSPLVSGADWQDLARQ
REASRALGVEGMMIKARAAQYGVGRTKDVGVWWKWKIDPYSVDAVLIYAQRGHGRRASLYTDYTFAVWDGEPGDHERKLV
PFAKAYSGLTDEEMRKVDAIVRKTTVEKFGPVRSVTPTLVFELGFEGIAASSRHKSGIAVRFPRMLRWRLDKPVEEADTL
ATLRELLG
>Mature_568_residues
MKAFATLYSRLDATTSSNAKLAAMRDYFREADPSDAAWAVYFLAGGRPRQLVPTRALRETAMQAAGLPEWLFEESYQAVG
DMAETISLLMSEAEHSSEDGLAVWMQDRLLPLRGLPPEELAERLPALWAQLDRLSLMVCIKLITGAFRVGVSKLLVTRAL
ASLADLDPKRVAQRLVGYTDLSHRPSAEGYRALIAEESEHEHAQRGGQPYPFFLAHALQAPVDTFDTLLGAPDNWFIEWK
WDGIRAQLVKRDGQIWVWSRGEELVSERFPELCELARCLPDGTVIDGEILVWKHDPETPAGELFDAASTGEPSADGFGVQ
PFALLQQRIGRKNLTAKVLQDAPVAVLAYDLLEWQGDDWRQREHRERRQQLEAVVGQCPSPQLMLSPLVSGADWQDLARQ
REASRALGVEGMMIKARAAQYGVGRTKDVGVWWKWKIDPYSVDAVLIYAQRGHGRRASLYTDYTFAVWDGEPGDHERKLV
PFAKAYSGLTDEEMRKVDAIVRKTTVEKFGPVRSVTPTLVFELGFEGIAASSRHKSGIAVRFPRMLRWRLDKPVEEADTL
ATLRELLG

Specific function: DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair [H]

COG id: COG1793

COG function: function code L; ATP-dependent DNA ligase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent DNA ligase family [H]

Homologues:

Organism=Homo sapiens, GI4557719, Length=396, Percent_Identity=23.989898989899, Blast_Score=76, Evalue=8e-14,
Organism=Caenorhabditis elegans, GI71985269, Length=582, Percent_Identity=24.7422680412371, Blast_Score=117, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6320038, Length=388, Percent_Identity=23.9690721649485, Blast_Score=92, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24762424, Length=384, Percent_Identity=24.7395833333333, Blast_Score=86, Evalue=6e-17,
Organism=Drosophila melanogaster, GI85815841, Length=371, Percent_Identity=23.4501347708895, Blast_Score=68, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022865
- InterPro:   IPR000977
- InterPro:   IPR012309
- InterPro:   IPR012310
- InterPro:   IPR016059
- InterPro:   IPR012308
- InterPro:   IPR012340
- InterPro:   IPR016027 [H]

Pfam domain/function: PF04679 DNA_ligase_A_C; PF01068 DNA_ligase_A_M; PF04675 DNA_ligase_A_N [H]

EC number: =6.5.1.1 [H]

Molecular weight: Translated: 63680; Mature: 63680

Theoretical pI: Translated: 5.59; Mature: 5.59

Prosite motif: PS00697 DNA_LIGASE_A1 ; PS50160 DNA_LIGASE_A3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAFATLYSRLDATTSSNAKLAAMRDYFREADPSDAAWAVYFLAGGRPRQLVPTRALRET
CCHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCCCCHHHHHHH
AMQAAGLPEWLFEESYQAVGDMAETISLLMSEAEHSSEDGLAVWMQDRLLPLRGLPPEEL
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCHHHH
AERLPALWAQLDRLSLMVCIKLITGAFRVGVSKLLVTRALASLADLDPKRVAQRLVGYTD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCC
LSHRPSAEGYRALIAEESEHEHAQRGGQPYPFFLAHALQAPVDTFDTLLGAPDNWFIEWK
CCCCCCCCHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHCCHHHHHHHHCCCCCCEEEEE
WDGIRAQLVKRDGQIWVWSRGEELVSERFPELCELARCLPDGTVIDGEILVWKHDPETPA
ECCHHHHHHHCCCCEEEEECCHHHHHHHCHHHHHHHHHCCCCCEECCEEEEEECCCCCCH
GELFDAASTGEPSADGFGVQPFALLQQRIGRKNLTAKVLQDAPVAVLAYDLLEWQGDDWR
HHHHCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHCCCHHHHHHHHHHCCCCHHH
QREHRERRQQLEAVVGQCPSPQLMLSPLVSGADWQDLARQREASRALGVEGMMIKARAAQ
HHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCEEEEEHHHH
YGVGRTKDVGVWWKWKIDPYSVDAVLIYAQRGHGRRASLYTDYTFAVWDGEPGDHERKLV
CCCCCCCCCCEEEEEEECCCCCCEEEEEEECCCCCEEEEEECEEEEEECCCCCCCCCCCC
PFAKAYSGLTDEEMRKVDAIVRKTTVEKFGPVRSVTPTLVFELGFEGIAASSRHKSGIAV
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCCCCCCCCEE
RFPRMLRWRLDKPVEEADTLATLRELLG
ECHHHHHHHCCCCHHHHHHHHHHHHHHC
>Mature Secondary Structure
MKAFATLYSRLDATTSSNAKLAAMRDYFREADPSDAAWAVYFLAGGRPRQLVPTRALRET
CCHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCCCCHHHHHHH
AMQAAGLPEWLFEESYQAVGDMAETISLLMSEAEHSSEDGLAVWMQDRLLPLRGLPPEEL
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCHHHH
AERLPALWAQLDRLSLMVCIKLITGAFRVGVSKLLVTRALASLADLDPKRVAQRLVGYTD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCC
LSHRPSAEGYRALIAEESEHEHAQRGGQPYPFFLAHALQAPVDTFDTLLGAPDNWFIEWK
CCCCCCCCHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHCCHHHHHHHHCCCCCCEEEEE
WDGIRAQLVKRDGQIWVWSRGEELVSERFPELCELARCLPDGTVIDGEILVWKHDPETPA
ECCHHHHHHHCCCCEEEEECCHHHHHHHCHHHHHHHHHCCCCCEECCEEEEEECCCCCCH
GELFDAASTGEPSADGFGVQPFALLQQRIGRKNLTAKVLQDAPVAVLAYDLLEWQGDDWR
HHHHCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHCCCHHHHHHHHHHCCCCHHH
QREHRERRQQLEAVVGQCPSPQLMLSPLVSGADWQDLARQREASRALGVEGMMIKARAAQ
HHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCEEEEEHHHH
YGVGRTKDVGVWWKWKIDPYSVDAVLIYAQRGHGRRASLYTDYTFAVWDGEPGDHERKLV
CCCCCCCCCCEEEEEEECCCCCCEEEEEEECCCCCEEEEEECEEEEEECCCCCCCCCCCC
PFAKAYSGLTDEEMRKVDAIVRKTTVEKFGPVRSVTPTLVFELGFEGIAASSRHKSGIAV
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCCCCCCCCEE
RFPRMLRWRLDKPVEEADTLATLRELLG
ECHHHHHHHCCCCHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA