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The map label for this gene is wcaJ [H]

Identifier: 146282832

GI number: 146282832

Start: 2716468

End: 2717895

Strand: Direct

Name: wcaJ [H]

Synonym: PST_2491

Alternate gene names: 146282832

Gene position: 2716468-2717895 (Clockwise)

Preceding gene: 146282831

Following gene: 146282833

Centisome position: 59.47

GC content: 62.82

Gene sequence:

>1428_bases
ATGCGCGTACAGTCTGTTGGAACCCTTGAATATGCTCACCCGAGTTTCGTCGATTACTTCCTTGCCAGCATTCGCCTGAT
CCACGGCCTCACGTCGATCCTGCCCGGACTACTCCTGCTGGCGTTCATGGAGCTTCCCGCGAGCGGCCCGTTCAGCGCAT
TCCTCGGCTTCTTCGCGGTACTCAGCGTCATCATCTTCCAGACCGTCGGCATCTATAGCGAAGAGGTATTCAGCACCCTG
TTGCGCTTCCGCGTCATGCTCGTGGCCTGGGCCGCAGCGTTCAGTTTCCTGATCTTCATGCATCAGGGCATGGGCCTGTT
CAGTTATCTGGAACCCAAACACCTGGTGTTCTGGTTCTTCACCAGCGCCGTCCTGTTCGGCGCCGAGCGCCTGGCCATGC
TCGCGCTGTTCCGCCGCCTGATGGCGCGGGGCATGTTCCTGCAGAACGCAGTGATCCTTGGCGGTACCGAGAACGGCCTG
CGCGTGGCCGAACACCTGCAGAACCATCGCGACATCCGCACCGGCGTGCTGGGCTTCATCGATGACCGCCTCGAGCGGTT
GCCGAAGGAACTGGCCAACCTGCCCCTGCTCGGCAACACCCGCGATCTGGAACGCATGATCCGCGAGGAAAAGGTCACCC
AGGTGCTGGTCGCCCTGCCCTGGTTCGCCGACAGCCGCATAGGCCAGCTGATCAACGAGCTGCGCAAGCTGCCGGTCAAC
GTCCTGCTGGTACCGGACATGGTCGCCTTCCGCCACGCCGACAAGCGCATCACCGAAGTCGGCGGACTGCCCACCCTGAT
CGCCTCGGACCTGCCGCTGCGCGGCTGGTCGCCCTTCTTCAAGCGGCTGGAAGACATCGTCATCTCCAGCATCGCCCTGC
TCGCTGCCGCGCCGGTGATGCTGCTCATCGCGCTGGCGATCAAACTCGACTCGCCGGGTCCGGTGCTCTTCAAGCAAAAG
CGCTACGGCTACAACAACCGGCTGATCGAAGTCTTCAAGTTCCGCTCGATGTACCACGAGAAGTCCGACGCCAACGCCGA
TCGGCAGACCACCCGTGGCGACGATCGCATCACCCGTGTCGGCCGCTTCATCCGCAAGACCAGCCTCGATGAGTTGCCAC
AGCTGCTGAACGTCTTCCTCGGCAGCATGTCCATGGTCGGTCCGCGCCCACATGCCACCGCCACCAAGGCTGCCGGCGTG
CTGTTCGAGGACGCCGTGGCCGAGTACTCGGCACGTCATCGCGTCAAACCCGGCATCACCGGCTGGGCGCAGATCAACGG
CTACCGCGGCGAGACCGACACGCTGGAGAAGATCGAGAAACGCGTCGAGTTCGACCTCGACTATATCGAGCGTTGGTCGG
TCTGGTTCGACCTCTACATCCTCGTACGCACCCCGCCGGCGCTGCTGCTGAACAAGGACGTGTACTGA

Upstream 100 bases:

>100_bases
CCCGATCACGGTTGCGATGGCGGCCTGCTATCAATCCCCCGCGTTCCGCGGCGATTAATTCGAGATTTGAGCCAGCTTTG
AGCTGAGGATGGAGCCTTGT

Downstream 100 bases:

>100_bases
TCGACGCACGCTCCGCGCGCCGCCGAAAAGACTGGAAAAAGTCACGCCGCACAAAGGCGTGACAGGAAGGAACTAATGAC
ACTGCCCAAAATCTCACATT

Product: capsular polysaccharide biosynthesis protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 475; Mature: 475

Protein sequence:

>475_residues
MRVQSVGTLEYAHPSFVDYFLASIRLIHGLTSILPGLLLLAFMELPASGPFSAFLGFFAVLSVIIFQTVGIYSEEVFSTL
LRFRVMLVAWAAAFSFLIFMHQGMGLFSYLEPKHLVFWFFTSAVLFGAERLAMLALFRRLMARGMFLQNAVILGGTENGL
RVAEHLQNHRDIRTGVLGFIDDRLERLPKELANLPLLGNTRDLERMIREEKVTQVLVALPWFADSRIGQLINELRKLPVN
VLLVPDMVAFRHADKRITEVGGLPTLIASDLPLRGWSPFFKRLEDIVISSIALLAAAPVMLLIALAIKLDSPGPVLFKQK
RYGYNNRLIEVFKFRSMYHEKSDANADRQTTRGDDRITRVGRFIRKTSLDELPQLLNVFLGSMSMVGPRPHATATKAAGV
LFEDAVAEYSARHRVKPGITGWAQINGYRGETDTLEKIEKRVEFDLDYIERWSVWFDLYILVRTPPALLLNKDVY

Sequences:

>Translated_475_residues
MRVQSVGTLEYAHPSFVDYFLASIRLIHGLTSILPGLLLLAFMELPASGPFSAFLGFFAVLSVIIFQTVGIYSEEVFSTL
LRFRVMLVAWAAAFSFLIFMHQGMGLFSYLEPKHLVFWFFTSAVLFGAERLAMLALFRRLMARGMFLQNAVILGGTENGL
RVAEHLQNHRDIRTGVLGFIDDRLERLPKELANLPLLGNTRDLERMIREEKVTQVLVALPWFADSRIGQLINELRKLPVN
VLLVPDMVAFRHADKRITEVGGLPTLIASDLPLRGWSPFFKRLEDIVISSIALLAAAPVMLLIALAIKLDSPGPVLFKQK
RYGYNNRLIEVFKFRSMYHEKSDANADRQTTRGDDRITRVGRFIRKTSLDELPQLLNVFLGSMSMVGPRPHATATKAAGV
LFEDAVAEYSARHRVKPGITGWAQINGYRGETDTLEKIEKRVEFDLDYIERWSVWFDLYILVRTPPALLLNKDVY
>Mature_475_residues
MRVQSVGTLEYAHPSFVDYFLASIRLIHGLTSILPGLLLLAFMELPASGPFSAFLGFFAVLSVIIFQTVGIYSEEVFSTL
LRFRVMLVAWAAAFSFLIFMHQGMGLFSYLEPKHLVFWFFTSAVLFGAERLAMLALFRRLMARGMFLQNAVILGGTENGL
RVAEHLQNHRDIRTGVLGFIDDRLERLPKELANLPLLGNTRDLERMIREEKVTQVLVALPWFADSRIGQLINELRKLPVN
VLLVPDMVAFRHADKRITEVGGLPTLIASDLPLRGWSPFFKRLEDIVISSIALLAAAPVMLLIALAIKLDSPGPVLFKQK
RYGYNNRLIEVFKFRSMYHEKSDANADRQTTRGDDRITRVGRFIRKTSLDELPQLLNVFLGSMSMVGPRPHATATKAAGV
LFEDAVAEYSARHRVKPGITGWAQINGYRGETDTLEKIEKRVEFDLDYIERWSVWFDLYILVRTPPALLLNKDVY

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: COG2148

COG function: function code M; Sugar transferases involved in lipopolysaccharide synthesis

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial sugar transferase family [H]

Homologues:

Organism=Escherichia coli, GI1788360, Length=278, Percent_Identity=47.841726618705, Blast_Score=268, Evalue=8e-73,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003362
- InterPro:   IPR017475
- InterPro:   IPR017473 [H]

Pfam domain/function: PF02397 Bac_transf [H]

EC number: NA

Molecular weight: Translated: 53835; Mature: 53835

Theoretical pI: Translated: 9.84; Mature: 9.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CHHEECCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHEECCCHHHCCCCCC
>Mature Secondary Structure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CHHEECCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHEECCCHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8759852; 9097040; 9278503 [H]