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The map label for this gene is alkA [H]

Identifier: 146282768

GI number: 146282768

Start: 2654614

End: 2655489

Strand: Direct

Name: alkA [H]

Synonym: PST_2427

Alternate gene names: 146282768

Gene position: 2654614-2655489 (Clockwise)

Preceding gene: 146282765

Following gene: 146282775

Centisome position: 58.12

GC content: 71.12

Gene sequence:

>876_bases
ATGACCGAAACCATCCACCTGCCCTACCTCGAACCCTGGGACTGGCAACAGTTCCACCGCCACTTCGCCCTGCGTCTGCT
GCCGGGCGTGGAGCGCCTGGACCTGCGCGGCTATGCCCGTACGCTGCGCCTGGGTGATGCCCGCGGCTGGCTCGGTGTGA
GCGCGGCGGACGACCGGCCGGCGCTCGAGCTGACCCTCAGCGACTCGCTGCGGCACGCCAGCCAACCGCTGGTGGCCCGG
GTGCGCAAGATGTTCGACCTGGATGCCGACCCGCAGGTGATCGCGGCGCATTTCGCCGGCGACCCGGCACTCGGCCCGCT
GGTCTCGGCACAACCCGGCCTGCGTCTGCCGGCGGCGTACGACCCGTTCGAGCAGGCCGTGCGCGCGGTGGTCGGCCAGC
AGGTCACGGTGAAGGCGGCGGTGACCATCACCCGCCGCCTGGTGGAGCGTCTCGGCGAGCCGTTGGCGCAGGCTCCGACC
GGGTTGGAGGTGCTGTTTCCCACGGCGCAGGCGATCGCCGACGACCCGCTGGAGAACATCGGCATGCCGGCCAAGCGCGC
TCAGGCGCTGCGCCGACTGGCCGGCGCGGTGGCCGAGGGCGCGTTGCACCTGCATGTGGAGGATGGCGCCGATACGCTGG
TCCGTCGGCTGTGCGAGCTGCCCGGCATCGGCCCCTGGACGGCCGAATACATCGCGCTGCGCGGTTTTGGCGTGGCAGAC
GCCTTTCCCAGTGCCGATCTGGGTTTACTCAAGGCGCCGCTTTGGGGAGCGGATGGCCTGACCGCCAAGGCGCTGGCCGA
ACGTGCCGAAGCCTGGCGCCCATGGCGGGCCTACGCAGCCATCCACATCTGGGATAACTACGCCCGGGGCGGCTGA

Upstream 100 bases:

>100_bases
GAGGCGCATTCTACGTTGGCCAAGATGACAGGGGGAACCGCGAGGGTTTGAAAGCACACTTCGGGGCGACAAAGCGGCGC
CCAGGCGCTAGGGTCTGGAA

Downstream 100 bases:

>100_bases
GGAAACGGTTACGGCGGCGCGACGCGCGCCAGCACCTTCGGCGCAACGGCCAGCGGCCGAGCCAGGATCAGCCCCTCGCC
CGACTGCGGAAAGATGCCGG

Product: DNA-3-methyladenine glycosidase II

Products: NA

Alternate protein names: 3-methyladenine-DNA glycosylase II, inducible; TAG II; DNA-3-methyladenine glycosidase II; DNA-3-methyladenine glycosylase II [H]

Number of amino acids: Translated: 291; Mature: 290

Protein sequence:

>291_residues
MTETIHLPYLEPWDWQQFHRHFALRLLPGVERLDLRGYARTLRLGDARGWLGVSAADDRPALELTLSDSLRHASQPLVAR
VRKMFDLDADPQVIAAHFAGDPALGPLVSAQPGLRLPAAYDPFEQAVRAVVGQQVTVKAAVTITRRLVERLGEPLAQAPT
GLEVLFPTAQAIADDPLENIGMPAKRAQALRRLAGAVAEGALHLHVEDGADTLVRRLCELPGIGPWTAEYIALRGFGVAD
AFPSADLGLLKAPLWGADGLTAKALAERAEAWRPWRAYAAIHIWDNYARGG

Sequences:

>Translated_291_residues
MTETIHLPYLEPWDWQQFHRHFALRLLPGVERLDLRGYARTLRLGDARGWLGVSAADDRPALELTLSDSLRHASQPLVAR
VRKMFDLDADPQVIAAHFAGDPALGPLVSAQPGLRLPAAYDPFEQAVRAVVGQQVTVKAAVTITRRLVERLGEPLAQAPT
GLEVLFPTAQAIADDPLENIGMPAKRAQALRRLAGAVAEGALHLHVEDGADTLVRRLCELPGIGPWTAEYIALRGFGVAD
AFPSADLGLLKAPLWGADGLTAKALAERAEAWRPWRAYAAIHIWDNYARGG
>Mature_290_residues
TETIHLPYLEPWDWQQFHRHFALRLLPGVERLDLRGYARTLRLGDARGWLGVSAADDRPALELTLSDSLRHASQPLVARV
RKMFDLDADPQVIAAHFAGDPALGPLVSAQPGLRLPAAYDPFEQAVRAVVGQQVTVKAAVTITRRLVERLGEPLAQAPTG
LEVLFPTAQAIADDPLENIGMPAKRAQALRRLAGAVAEGALHLHVEDGADTLVRRLCELPGIGPWTAEYIALRGFGVADA
FPSADLGLLKAPLWGADGLTAKALAERAEAWRPWRAYAAIHIWDNYARGG

Specific function: Hydrolysis of the deoxyribose N-glycosidic bond to excise 3-methyladenine, 3-methylguanine, 7-methylguanine, O2- methylthymine, and O2-methylcytosine from the damaged DNA polymer formed by alkylation lesions [H]

COG id: COG0122

COG function: function code L; 3-methyladenine DNA glycosylase/8-oxoguanine DNA glycosylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alkylbase DNA glycosidase alkA family [H]

Homologues:

Organism=Escherichia coli, GI1788383, Length=281, Percent_Identity=38.4341637010676, Blast_Score=167, Evalue=1e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010316
- InterPro:   IPR000035
- InterPro:   IPR011257
- InterPro:   IPR003265
- InterPro:   IPR023170
- InterPro:   IPR012294 [H]

Pfam domain/function: PF06029 AlkA_N; PF00730 HhH-GPD [H]

EC number: =3.2.2.21 [H]

Molecular weight: Translated: 31678; Mature: 31546

Theoretical pI: Translated: 6.73; Mature: 6.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTETIHLPYLEPWDWQQFHRHFALRLLPGVERLDLRGYARTLRLGDARGWLGVSAADDRP
CCCEEECCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHEECCCCCCEECCCCCCCC
ALELTLSDSLRHASQPLVARVRKMFDLDADPQVIAAHFAGDPALGPLVSAQPGLRLPAAY
CEEEEECHHHHHCCCHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCC
DPFEQAVRAVVGQQVTVKAAVTITRRLVERLGEPLAQAPTGLEVLFPTAQAIADDPLENI
CHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHCCHHHHCCCCCEEEECCHHHHHCCCHHHC
GMPAKRAQALRRLAGAVAEGALHLHVEDGADTLVRRLCELPGIGPWTAEYIALRGFGVAD
CCCHHHHHHHHHHHHHHHCCEEEEEEECCHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCC
AFPSADLGLLKAPLWGADGLTAKALAERAEAWRPWRAYAAIHIWDNYARGG
CCCCCCCHHHCCCCCCCCCCHHHHHHHHHHHCCCHHHEEEEEEECCCCCCC
>Mature Secondary Structure 
TETIHLPYLEPWDWQQFHRHFALRLLPGVERLDLRGYARTLRLGDARGWLGVSAADDRP
CCEEECCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHEECCCCCCEECCCCCCCC
ALELTLSDSLRHASQPLVARVRKMFDLDADPQVIAAHFAGDPALGPLVSAQPGLRLPAAY
CEEEEECHHHHHCCCHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCC
DPFEQAVRAVVGQQVTVKAAVTITRRLVERLGEPLAQAPTGLEVLFPTAQAIADDPLENI
CHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHCCHHHHCCCCCEEEECCHHHHHCCCHHHC
GMPAKRAQALRRLAGAVAEGALHLHVEDGADTLVRRLCELPGIGPWTAEYIALRGFGVAD
CCCHHHHHHHHHHHHHHHCCEEEEEEECCHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCC
AFPSADLGLLKAPLWGADGLTAKALAERAEAWRPWRAYAAIHIWDNYARGG
CCCCCCCHHHCCCCCCCCCCHHHHHHHHHHHCCCHHHEEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 6094528; 9097040; 9278503; 6389535; 3529081; 8706135; 8706136 [H]