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The map label for this gene is nasA [H]

Identifier: 146282747

GI number: 146282747

Start: 2627881

End: 2629092

Strand: Direct

Name: nasA [H]

Synonym: PST_2406

Alternate gene names: 146282747

Gene position: 2627881-2629092 (Clockwise)

Preceding gene: 146282746

Following gene: 146282748

Centisome position: 57.54

GC content: 66.83

Gene sequence:

>1212_bases
ATGAGCACAAGTTTCTGGAAAGCCGGCCACGCGCCTACCCTGTTTGCCTCCTTTCTCTATTTCGACCTGAGCTTCATGGT
CTGGTACGTGCTCGGCCCGCTCGGCGTGCAGATCGCCGCCGACCTGGGCCTGACCACCCAGCAACGCGCGTTCATGGTGG
CGACGCCGATCCTCGCCGGCGCCGTGCTACGACTGGCGCTTGGCATCCTCGCCGACCGCACCTCACCGAAGACCGCCGGG
CTGTTCGGCCAGAGCGTGGTCATCGTTGCCCTGCTAGTGGCCTGGTTGCACGGCATCAACAGCTATGAGCAGGCGCTGCT
GCTCGGCCTGTTCCTCGGCATGGCTGGCGCCTCCTTCGCCGTGGCGCTGCCGCTAGCCTCGCAGTGGTATCCGGCGGAAC
ATCAGGGCAAGGCCATGGGCATCGCCGGCGCGGGCAACTCCGGCACCGTGCTGGCGGCCCTGTTCGCCCCGGTGCTGGCG
GCGATCTTCGGCTGGAACAATGTGTTCGGCCTGGCCCTGATCCCGCTGGTACTCACGCTCATCATCTTCGCCAGCGTGGC
GAAGAACGCGCCCAACCGCCCGGCACCCAAGTCGTTGGCGGACTACATGAAGGCACTAGGCGACCGCGACAGCTGGTGGT
TCATGTTCTTCTACAGCGTGACCTTTGGCGGCTTCCTCGGCCTGGCCAGCACCCTGCCCGGCTATTTCCACGACCAGTAC
GCCTTCGACCCGGTCAAGGCCGGCTATTACACCGCCGCCTGCGTGTTCGCCGGCAGCCTGCTGCGCCCACTCGGCGGTGC
CCTGGCCGACCGCATCGGCGGCATTCGCGCGCTGCTGGTGATGTACACGGTCGCGGCCATCTGTATTGCCGCTGTCGGCT
TCAACCTGCCCAGTTCCACGGCCGCCCTGGCGCTGTTCGTGGTCGCCATGCTCAGCCTCGGTGCCGGAAACGGTGCGGTG
TTCCAGCTGGTGCCGCAGCGTTTCAACAAGGAAATAGGCGTGATGACCGGGTTGGTCGGCATGGCTGGCGGTATCGGTGG
CTTCCTGCTCACCGCCGGGCTGGGCGCGGTCAAGCAGTCCACCGGCGATTACCAGTTCGGCCTCTGGCTGTTCGCCAGCC
TCGGTGTGCTCGCCTGGTTCGGCCTGCATGGCGTCAAGCGTCGCTGGCGCACCACCTGGGGTTCGGCCGCCGTCACCGCC
GCGCGGGTCTGA

Upstream 100 bases:

>100_bases
GCGTGCTGCGGCGCCTTTTTGTTTTCCGCCTCGCCGATGGGGCGGATCGAGCAGCCCTGGCGGCCAACGCCGCGCCACGT
AACGCCCTGGAGTCGATCCA

Downstream 100 bases:

>100_bases
TGCCGATGCCGCAGGGCGCGATTATTGCTGCGCCGCCGTACCGAGCCTTTCGAGAACGATCATGCCCCTGCGATTGAGTT
ACGGCGAGGCCAGCGCCGCC

Product: nitrate transporter

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 403; Mature: 402

Protein sequence:

>403_residues
MSTSFWKAGHAPTLFASFLYFDLSFMVWYVLGPLGVQIAADLGLTTQQRAFMVATPILAGAVLRLALGILADRTSPKTAG
LFGQSVVIVALLVAWLHGINSYEQALLLGLFLGMAGASFAVALPLASQWYPAEHQGKAMGIAGAGNSGTVLAALFAPVLA
AIFGWNNVFGLALIPLVLTLIIFASVAKNAPNRPAPKSLADYMKALGDRDSWWFMFFYSVTFGGFLGLASTLPGYFHDQY
AFDPVKAGYYTAACVFAGSLLRPLGGALADRIGGIRALLVMYTVAAICIAAVGFNLPSSTAALALFVVAMLSLGAGNGAV
FQLVPQRFNKEIGVMTGLVGMAGGIGGFLLTAGLGAVKQSTGDYQFGLWLFASLGVLAWFGLHGVKRRWRTTWGSAAVTA
ARV

Sequences:

>Translated_403_residues
MSTSFWKAGHAPTLFASFLYFDLSFMVWYVLGPLGVQIAADLGLTTQQRAFMVATPILAGAVLRLALGILADRTSPKTAG
LFGQSVVIVALLVAWLHGINSYEQALLLGLFLGMAGASFAVALPLASQWYPAEHQGKAMGIAGAGNSGTVLAALFAPVLA
AIFGWNNVFGLALIPLVLTLIIFASVAKNAPNRPAPKSLADYMKALGDRDSWWFMFFYSVTFGGFLGLASTLPGYFHDQY
AFDPVKAGYYTAACVFAGSLLRPLGGALADRIGGIRALLVMYTVAAICIAAVGFNLPSSTAALALFVVAMLSLGAGNGAV
FQLVPQRFNKEIGVMTGLVGMAGGIGGFLLTAGLGAVKQSTGDYQFGLWLFASLGVLAWFGLHGVKRRWRTTWGSAAVTA
ARV
>Mature_402_residues
STSFWKAGHAPTLFASFLYFDLSFMVWYVLGPLGVQIAADLGLTTQQRAFMVATPILAGAVLRLALGILADRTSPKTAGL
FGQSVVIVALLVAWLHGINSYEQALLLGLFLGMAGASFAVALPLASQWYPAEHQGKAMGIAGAGNSGTVLAALFAPVLAA
IFGWNNVFGLALIPLVLTLIIFASVAKNAPNRPAPKSLADYMKALGDRDSWWFMFFYSVTFGGFLGLASTLPGYFHDQYA
FDPVKAGYYTAACVFAGSLLRPLGGALADRIGGIRALLVMYTVAAICIAAVGFNLPSSTAALALFVVAMLSLGAGNGAVF
QLVPQRFNKEIGVMTGLVGMAGGIGGFLLTAGLGAVKQSTGDYQFGLWLFASLGVLAWFGLHGVKRRWRTTWGSAAVTAA
RV

Specific function: May function as a nitrate transporter [H]

COG id: COG2223

COG function: function code P; Nitrate/nitrite transporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Nitrate/nitrite porter (TC 2.A.1.8) family [H]

Homologues:

Organism=Escherichia coli, GI1787475, Length=421, Percent_Identity=25.41567695962, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1787743, Length=394, Percent_Identity=23.8578680203046, Blast_Score=64, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004737 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 42312; Mature: 42181

Theoretical pI: Translated: 10.04; Mature: 10.04

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTSFWKAGHAPTLFASFLYFDLSFMVWYVLGPLGVQIAADLGLTTQQRAFMVATPILAG
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
AVLRLALGILADRTSPKTAGLFGQSVVIVALLVAWLHGINSYEQALLLGLFLGMAGASFA
HHHHHHHHHHHCCCCCCHHHHCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHH
VALPLASQWYPAEHQGKAMGIAGAGNSGTVLAALFAPVLAAIFGWNNVFGLALIPLVLTL
EEECHHHCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
IIFASVAKNAPNRPAPKSLADYMKALGDRDSWWFMFFYSVTFGGFLGLASTLPGYFHDQY
HHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHCCCC
AFDPVKAGYYTAACVFAGSLLRPLGGALADRIGGIRALLVMYTVAAICIAAVGFNLPSST
CCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
AALALFVVAMLSLGAGNGAVFQLVPQRFNKEIGVMTGLVGMAGGIGGFLLTAGLGAVKQS
HHHHHHHHHHHHCCCCCCHHHHHHHHHHCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHC
TGDYQFGLWLFASLGVLAWFGLHGVKRRWRTTWGSAAVTAARV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCC
>Mature Secondary Structure 
STSFWKAGHAPTLFASFLYFDLSFMVWYVLGPLGVQIAADLGLTTQQRAFMVATPILAG
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
AVLRLALGILADRTSPKTAGLFGQSVVIVALLVAWLHGINSYEQALLLGLFLGMAGASFA
HHHHHHHHHHHCCCCCCHHHHCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHH
VALPLASQWYPAEHQGKAMGIAGAGNSGTVLAALFAPVLAAIFGWNNVFGLALIPLVLTL
EEECHHHCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
IIFASVAKNAPNRPAPKSLADYMKALGDRDSWWFMFFYSVTFGGFLGLASTLPGYFHDQY
HHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHCCCC
AFDPVKAGYYTAACVFAGSLLRPLGGALADRIGGIRALLVMYTVAAICIAAVGFNLPSST
CCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
AALALFVVAMLSLGAGNGAVFQLVPQRFNKEIGVMTGLVGMAGGIGGFLLTAGLGAVKQS
HHHHHHHHHHHHCCCCCCHHHHHHHHHHCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHC
TGDYQFGLWLFASLGVLAWFGLHGVKRRWRTTWGSAAVTAARV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7868621; 8969502; 9384377 [H]