| Definition | Pseudomonas stutzeri A1501, complete genome. |
|---|---|
| Accession | NC_009434 |
| Length | 4,567,418 |
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The map label for this gene is dacB [H]
Identifier: 146282728
GI number: 146282728
Start: 2604295
End: 2605752
Strand: Direct
Name: dacB [H]
Synonym: PST_2387
Alternate gene names: 146282728
Gene position: 2604295-2605752 (Clockwise)
Preceding gene: 146282726
Following gene: 146282730
Centisome position: 57.02
GC content: 63.92
Gene sequence:
>1458_bases ATGAACAGAATCCTTCGTGGTATCTCCCTGGCCGTTCTGGCCTTTCCCATTGCGCTGCCGATTCATGCGGCCGGCGTGAA CCAGACGCTGCCTGCGCGTGTGGAACAGGCGCTCAAGGCCAACAAGATCAACAACGATGCGCTGTCGGTCGTCATGCTGC CGCTTACCGGCAACGCGGCACCGACAATCGTCAATGCAGACGTATCGGTCAATCCCGCCTCGACCATGAAATTGGTCACC ACCTACGCCGCACTCGAACTGCTCGGGCCCAATCATCAGTGGAAAACCGAGTTCTATGCCGACGGCCCGCTGGTGGATGG CGTGCTCAATGGCAATCTTTATCTGAAAGGTGGCGGCGATCCCAAGCTGAACATGGAGAAGCTCTGGTTGCTGCTGCGTG ATCTGCGCGCCAACGGTGTGAGCAAGGTCACCGGCGATCTGGTGCTGGATCGCAGCCACTTCGTCCAGCCCGACCTGCCG GTATTCGACGATGACGGCGGCGACCAGAACAAGCCGTTCCTCGTCGGCCCCGATTCGCTACTGGTCAACCTCAAGGCGCT GCGCTTCATCGTCCGCAACGATGGGGGCAAGGTGAACGTGGCGGTGGAGCCGCCCATTGCCAGCGTCCGCATCGACAACC GCGTTCAGGCCTTGCCGGCAGCCAAGTGCCCAGGCTGGCCAGAGGTGCTGTACAACCCCGTTGCCGACGGCGACGGGGTC ACCGTGGTGGTCACGGGCAAGCTCGCCGCTGGTTGCAGCGCGCAGACCTATCTTTCCCTGCTGGACCACGAGCGCTATGC CGCAGGCGCGGTGCGGGCGATCTGGGGCGAACTCGGCGGCAGCATCCTGGGCACTGATCGTGTCGCCCAGGTACCGAGCA GCGCCCGCCTGCTGGCCAGGGCCTACTCCCCGGACCTCACCGAGATCATTCGCGACGTCAACAAGTACAGCAACAACACC ATGGCCAAGCAGCTGTTCCTGAGCCTGGGTGCTGCGTTCCGCAACGAAGCGGATGCCGATGACGCCAAGGCGGCACAGCG AGTGATCCGTCAGTGGCTGGCCAAGAAGGGGCTGATTTCCCCGCACCTGGTGATCGAAAACGGCTCCGGCCTTTCCCGTG CCGAGCGGATCAGCGCGCGTGAACTGGCGAGTTTGCTGCAGGCCGCCTGGCGCAGCCCCTACTCGGCCGAGTTCATCTCC TCGATGCCCTTGGCGGCCGTGGACGGCACCATGCGCAAGCGTCTGCGCAACACGCCGGTCGCCGGCAAGGCACACGTGAA GACGGGCACGCTGAATACCGTGCGCGCGATCGCGGGCTACAGTCGAGACGGCGACGGCAATGTCTGGGCGGTGGTGGCGA TCCTCAACCACCCGCGTCCATGGGGCGCCTCGTCGATCCTGGATCAGGTGCTGGTCAGCCTGTACAACCAGCGCAGAGAA AGCACCGCTCAGCGCTGA
Upstream 100 bases:
>100_bases CGGGCATAATGGCCCGCAATCGTTCCCTTAGTGCAGTGGGAAATCGCATGCCGAATCGAATCGCCAGCAGCCCCCTGTGT GCGCTCAGAGGCGCTCGCAA
Downstream 100 bases:
>100_bases TCGCTACAGGAAGAGCGGGCCTGCCTGAGGCCCGCTCAGAACCGTCATCACCGTGCCTGGAGTTTCCAGGCACGGTGGAT TCGCGGATTGCGGCGGAAGT
Product: D-alanyl-D-alanine carboxypeptidase
Products: NA
Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein; PBP [H]
Number of amino acids: Translated: 485; Mature: 485
Protein sequence:
>485_residues MNRILRGISLAVLAFPIALPIHAAGVNQTLPARVEQALKANKINNDALSVVMLPLTGNAAPTIVNADVSVNPASTMKLVT TYAALELLGPNHQWKTEFYADGPLVDGVLNGNLYLKGGGDPKLNMEKLWLLLRDLRANGVSKVTGDLVLDRSHFVQPDLP VFDDDGGDQNKPFLVGPDSLLVNLKALRFIVRNDGGKVNVAVEPPIASVRIDNRVQALPAAKCPGWPEVLYNPVADGDGV TVVVTGKLAAGCSAQTYLSLLDHERYAAGAVRAIWGELGGSILGTDRVAQVPSSARLLARAYSPDLTEIIRDVNKYSNNT MAKQLFLSLGAAFRNEADADDAKAAQRVIRQWLAKKGLISPHLVIENGSGLSRAERISARELASLLQAAWRSPYSAEFIS SMPLAAVDGTMRKRLRNTPVAGKAHVKTGTLNTVRAIAGYSRDGDGNVWAVVAILNHPRPWGASSILDQVLVSLYNQRRE STAQR
Sequences:
>Translated_485_residues MNRILRGISLAVLAFPIALPIHAAGVNQTLPARVEQALKANKINNDALSVVMLPLTGNAAPTIVNADVSVNPASTMKLVT TYAALELLGPNHQWKTEFYADGPLVDGVLNGNLYLKGGGDPKLNMEKLWLLLRDLRANGVSKVTGDLVLDRSHFVQPDLP VFDDDGGDQNKPFLVGPDSLLVNLKALRFIVRNDGGKVNVAVEPPIASVRIDNRVQALPAAKCPGWPEVLYNPVADGDGV TVVVTGKLAAGCSAQTYLSLLDHERYAAGAVRAIWGELGGSILGTDRVAQVPSSARLLARAYSPDLTEIIRDVNKYSNNT MAKQLFLSLGAAFRNEADADDAKAAQRVIRQWLAKKGLISPHLVIENGSGLSRAERISARELASLLQAAWRSPYSAEFIS SMPLAAVDGTMRKRLRNTPVAGKAHVKTGTLNTVRAIAGYSRDGDGNVWAVVAILNHPRPWGASSILDQVLVSLYNQRRE STAQR >Mature_485_residues MNRILRGISLAVLAFPIALPIHAAGVNQTLPARVEQALKANKINNDALSVVMLPLTGNAAPTIVNADVSVNPASTMKLVT TYAALELLGPNHQWKTEFYADGPLVDGVLNGNLYLKGGGDPKLNMEKLWLLLRDLRANGVSKVTGDLVLDRSHFVQPDLP VFDDDGGDQNKPFLVGPDSLLVNLKALRFIVRNDGGKVNVAVEPPIASVRIDNRVQALPAAKCPGWPEVLYNPVADGDGV TVVVTGKLAAGCSAQTYLSLLDHERYAAGAVRAIWGELGGSILGTDRVAQVPSSARLLARAYSPDLTEIIRDVNKYSNNT MAKQLFLSLGAAFRNEADADDAKAAQRVIRQWLAKKGLISPHLVIENGSGLSRAERISARELASLLQAAWRSPYSAEFIS SMPLAAVDGTMRKRLRNTPVAGKAHVKTGTLNTVRAIAGYSRDGDGNVWAVVAILNHPRPWGASSILDQVLVSLYNQRRE STAQR
Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors [H]
COG id: COG2027
COG function: function code M; D-alanyl-D-alanine carboxypeptidase (penicillin-binding protein 4)
Gene ontology:
Cell location: Secreted [H]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the peptidase S13 family [H]
Homologues:
Organism=Escherichia coli, GI1789573, Length=464, Percent_Identity=27.5862068965517, Blast_Score=112, Evalue=4e-26,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR012338 - InterPro: IPR000667 [H]
Pfam domain/function: PF02113 Peptidase_S13 [H]
EC number: =3.4.16.4 [H]
Molecular weight: Translated: 52110; Mature: 52110
Theoretical pI: Translated: 9.85; Mature: 9.85
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.4 %Cys (Translated Protein) 1.4 %Met (Translated Protein) 1.9 %Cys+Met (Translated Protein) 0.4 %Cys (Mature Protein) 1.4 %Met (Mature Protein) 1.9 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MNRILRGISLAVLAFPIALPIHAAGVNQTLPARVEQALKANKINNDALSVVMLPLTGNAA CCHHHHHHHHHHHHHHHHHHEEECCCCCCCHHHHHHHHHHCCCCCCCEEEEEEEECCCCC PTIVNADVSVNPASTMKLVTTYAALELLGPNHQWKTEFYADGPLVDGVLNGNLYLKGGGD CEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCEECEEECCEEEEECCCC PKLNMEKLWLLLRDLRANGVSKVTGDLVLDRSHFVQPDLPVFDDDGGDQNKPFLVGPDSL CCCCHHHHHHHHHHHHCCCCHHHHHHEEECCCCCCCCCCCCCCCCCCCCCCCEEECCHHH LVNLKALRFIVRNDGGKVNVAVEPPIASVRIDNRVQALPAAKCPGWPEVLYNPVADGDGV HHHHHHHEEEEECCCCEEEEEECCCCEEEEECCCHHHCCCCCCCCCHHHHCCCCCCCCCE TVVVTGKLAAGCSAQTYLSLLDHERYAAGAVRAIWGELGGSILGTDRVAQVPSSARLLAR EEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCHHHHCCCHHHHHHH AYSPDLTEIIRDVNKYSNNTMAKQLFLSLGAAFRNEADADDAKAAQRVIRQWLAKKGLIS HCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCC PHLVIENGSGLSRAERISARELASLLQAAWRSPYSAEFISSMPLAAVDGTMRKRLRNTPV CEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCHHHHHHHHHHHHCCCC AGKAHVKTGTLNTVRAIAGYSRDGDGNVWAVVAILNHPRPWGASSILDQVLVSLYNQRRE CCCEEEECCCCHHHHHHHCCCCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHH STAQR HHCCC >Mature Secondary Structure MNRILRGISLAVLAFPIALPIHAAGVNQTLPARVEQALKANKINNDALSVVMLPLTGNAA CCHHHHHHHHHHHHHHHHHHEEECCCCCCCHHHHHHHHHHCCCCCCCEEEEEEEECCCCC PTIVNADVSVNPASTMKLVTTYAALELLGPNHQWKTEFYADGPLVDGVLNGNLYLKGGGD CEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCEECEEECCEEEEECCCC PKLNMEKLWLLLRDLRANGVSKVTGDLVLDRSHFVQPDLPVFDDDGGDQNKPFLVGPDSL CCCCHHHHHHHHHHHHCCCCHHHHHHEEECCCCCCCCCCCCCCCCCCCCCCCEEECCHHH LVNLKALRFIVRNDGGKVNVAVEPPIASVRIDNRVQALPAAKCPGWPEVLYNPVADGDGV HHHHHHHEEEEECCCCEEEEEECCCCEEEEECCCHHHCCCCCCCCCHHHHCCCCCCCCCE TVVVTGKLAAGCSAQTYLSLLDHERYAAGAVRAIWGELGGSILGTDRVAQVPSSARLLAR EEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCHHHHCCCHHHHHHH AYSPDLTEIIRDVNKYSNNTMAKQLFLSLGAAFRNEADADDAKAAQRVIRQWLAKKGLIS HCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCC PHLVIENGSGLSRAERISARELASLLQAAWRSPYSAEFISSMPLAAVDGTMRKRLRNTPV CEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCHHHHHHHHHHHHCCCC AGKAHVKTGTLNTVRAIAGYSRDGDGNVWAVVAILNHPRPWGASSILDQVLVSLYNQRRE CCCEEEECCCCHHHHHHHCCCCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHH STAQR HHCCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 1554361; 7305936 [H]