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The map label for this gene is ampS [H]

Identifier: 145301168

GI number: 145301168

Start: 4651259

End: 4652023

Strand: Direct

Name: ampS [H]

Synonym: ASA_4346

Alternate gene names: 145301168

Gene position: 4651259-4652023 (Clockwise)

Preceding gene: 145301167

Following gene: 145301169

Centisome position: 98.91

GC content: 62.22

Gene sequence:

>765_bases
ATGCTGCCGCCTCCCGGCCCTTTGTCCCATTCTCCAAGGAGACCCCATGTCCCGGATCCTGTTACCCGGCCTGCTCGCAG
CAGGCCTGATATTCTCACTGCCAGCCAGTTGCCACCCGCCTCCACCTTCAAGATACCGCTGGCGCTGATGGGTTATGACA
GCGGCTTCCTGGTGGATGAAAATCTGCCCGCTCTGCCATTCAAACCCGGCGACGATGACTGGTTGCCCGCCTGGCGCGAG
ACCACCACCCCGAGCCGCTGGCTCACCTACTCTGTGGTCTGGTACTCCCAGCGTCTCACAGAGTGGCTGGGGATGGAGCG
CTTCCAGCAGTATGTCGACCGCTTCGACTACGGCAACCGTGATCTTGCGGGCAATCCCGGCAAGCACGATGGTCTGACCC
AGGCCTGGCTCAGTTCGAGCCTCGCCATCAGCCCCCAGGAGCAGGCCCGCTTCCTCGGCAAGATGGTGAGCGGCAAGTTG
CCGGTCTCCACACAGACACTGCAGCACACGGCCAATATCCTGCGCCAGCCCGACATCGATGGCTGGCAGATCCACGGCAA
GACCGGCATGGGCTACCCCAAGCTGCTGGATGGCACCCTCAATCGGGATCAGCAGATCGGCTGGTTCGTCGGCTGGGCCA
GCAAACCGGGCAAGCAGCTCATCTTTGTCCACACTGTCGTGCAAAAGCCCGGCAAGCAATTCGCCTCGCTCAAGGCGAAG
GAAGAGGTGTTTGCCGCCCTGCCTGACCAGCTGAAGAAGCTGTGA

Upstream 100 bases:

>100_bases
TGGTCGGCTGAACCTCTGCAACCAGCCAGAAGGTGGCGTGCTGGCCGAACTCTGGCTGCCAGCAGGCGGACACTTCACAT
TGGGCACATAAAGCGGCGCT

Downstream 100 bases:

>100_bases
TCCCAGAACCGGCGCAAGGCGCCGGTTTTTTTATCACCCTTCACTCCCGCTTCACACGGCCCCATCTCCTCTTCACCCAA
GCCCGGCACACTTGTTCCCG

Product: AmpS beta-lactamase

Products: NA

Alternate protein names: Penicillinase [H]

Number of amino acids: Translated: 254; Mature: 254

Protein sequence:

>254_residues
MLPPPGPLSHSPRRPHVPDPVTRPARSRPDILTASQLPPASTFKIPLALMGYDSGFLVDENLPALPFKPGDDDWLPAWRE
TTTPSRWLTYSVVWYSQRLTEWLGMERFQQYVDRFDYGNRDLAGNPGKHDGLTQAWLSSSLAISPQEQARFLGKMVSGKL
PVSTQTLQHTANILRQPDIDGWQIHGKTGMGYPKLLDGTLNRDQQIGWFVGWASKPGKQLIFVHTVVQKPGKQFASLKAK
EEVFAALPDQLKKL

Sequences:

>Translated_254_residues
MLPPPGPLSHSPRRPHVPDPVTRPARSRPDILTASQLPPASTFKIPLALMGYDSGFLVDENLPALPFKPGDDDWLPAWRE
TTTPSRWLTYSVVWYSQRLTEWLGMERFQQYVDRFDYGNRDLAGNPGKHDGLTQAWLSSSLAISPQEQARFLGKMVSGKL
PVSTQTLQHTANILRQPDIDGWQIHGKTGMGYPKLLDGTLNRDQQIGWFVGWASKPGKQLIFVHTVVQKPGKQFASLKAK
EEVFAALPDQLKKL
>Mature_254_residues
MLPPPGPLSHSPRRPHVPDPVTRPARSRPDILTASQLPPASTFKIPLALMGYDSGFLVDENLPALPFKPGDDDWLPAWRE
TTTPSRWLTYSVVWYSQRLTEWLGMERFQQYVDRFDYGNRDLAGNPGKHDGLTQAWLSSSLAISPQEQARFLGKMVSGKL
PVSTQTLQHTANILRQPDIDGWQIHGKTGMGYPKLLDGTLNRDQQIGWFVGWASKPGKQLIFVHTVVQKPGKQFASLKAK
EEVFAALPDQLKKL

Specific function: Has a broad substrate profile, hydrolyzes amoxicillin, ticarcillin, cephalothin, ceftazidime, cefotaxime, and aztreonam, but not imipenem or cephamycins [H]

COG id: COG2602

COG function: function code V; Beta-lactamase class D

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-D beta-lactamase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR002137
- InterPro:   IPR001460 [H]

Pfam domain/function: PF00905 Transpeptidase [H]

EC number: =3.5.2.6 [H]

Molecular weight: Translated: 28503; Mature: 28503

Theoretical pI: Translated: 10.04; Mature: 10.04

Prosite motif: PS00337 BETA_LACTAMASE_D

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLPPPGPLSHSPRRPHVPDPVTRPARSRPDILTASQLPPASTFKIPLALMGYDSGFLVDE
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCEECEEEEEECCCCEEECC
NLPALPFKPGDDDWLPAWRETTTPSRWLTYSVVWYSQRLTEWLGMERFQQYVDRFDYGNR
CCCCCCCCCCCCCCCCCCCCCCCCCHHEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
DLAGNPGKHDGLTQAWLSSSLAISPQEQARFLGKMVSGKLPVSTQTLQHTANILRQPDID
CCCCCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCC
GWQIHGKTGMGYPKLLDGTLNRDQQIGWFVGWASKPGKQLIFVHTVVQKPGKQFASLKAK
CEEEECCCCCCCHHHHCCCCCCCCCCEEEEEECCCCCCEEEEEEHHHHCCCHHHHHHHHH
EEVFAALPDQLKKL
HHHHHHHHHHHHCC
>Mature Secondary Structure
MLPPPGPLSHSPRRPHVPDPVTRPARSRPDILTASQLPPASTFKIPLALMGYDSGFLVDE
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCEECEEEEEECCCCEEECC
NLPALPFKPGDDDWLPAWRETTTPSRWLTYSVVWYSQRLTEWLGMERFQQYVDRFDYGNR
CCCCCCCCCCCCCCCCCCCCCCCCCHHEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
DLAGNPGKHDGLTQAWLSSSLAISPQEQARFLGKMVSGKLPVSTQTLQHTANILRQPDID
CCCCCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCC
GWQIHGKTGMGYPKLLDGTLNRDQQIGWFVGWASKPGKQLIFVHTVVQKPGKQFASLKAK
CEEEECCCCCCCHHHHCCCCCCCCCCEEEEEECCCCCCEEEEEEHHHHCCCHHHHHHHHH
EEVFAALPDQLKKL
HHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9333046 [H]