Click here to switch to the map view.

The map label for this gene is ycgF [H]

Identifier: 145301096

GI number: 145301096

Start: 4561437

End: 4562201

Strand: Direct

Name: ycgF [H]

Synonym: ASA_4265

Alternate gene names: 145301096

Gene position: 4561437-4562201 (Clockwise)

Preceding gene: 145301095

Following gene: 145301098

Centisome position: 97.0

GC content: 54.64

Gene sequence:

>765_bases
ATGGACATACCTTCTGCTCCCCCCCAGCTATCGTGCCAACAGTGCCAGGAAAGCGGGGATCTCGGCTTCGATTTCACCAT
GGCCTTTCAACCCATAATCCGCTGTCGTACCCGTACCATATTTGGCTATGAAGCGCTGGTCAGGGGGATCAATAACGAAC
CCGCCCACTCAGTGATTTCACGGGTCAACGATGACAATCGCTACCGCTTTGATCAGCTTTGCCGGATCAAAGCCATTGCA
CTGGCGGCCAAACTCAAGATGCAGGGAATGCTGAGCATCAATTTCCTGCCCAATGCGGTTTATCGTCCGGAGCGCTGCAT
CCGCACCACCTTGCAAGCCGCGCGGGAGCACCAGTTTCCCATCGAGCAGATCATGTTCGAGTTCACCGAAGTGGAGCGCG
TGGCGGATAACCAGCATATTCGCCACATAGTGGATCACTATCAGGCACTCGGTTTCAAGACGGCGATGGATGACTTTGGC
GCCGGATATTCCAGTCTCAATCTGCTGGCAGATTTTCTGCCAGACATCATCAAGCTGGATATGCATCTGTTGAGAGGGAT
CGATAAAGACAAGGTCCGCCAGGCCATTGTGCGCCACTGTCTCGCCCTCTTTGAGGAGTTGAACATCACCCCCCTGGCGG
AAGGGATCGAAACCCGGGATGAAATGGCATGTCTGCAGGAGATGGGGATAGACCTGATGCAGGGTTATCTGTTTGCCCGC
CCCGGCTTTGAAACCCTGCCCGAGGTGGATTTCGTCAGCCTGTAA

Upstream 100 bases:

>100_bases
AGACCGTTTTCCGGGCGCCATCCAGGCTTGTTATCATTTGAGGCTGGATATAGGCTTGATATCGCGCTCCGGCTCACCAG
TCCCCCTGTATGAGGAAAGC

Downstream 100 bases:

>100_bases
GTCATATCGGCCTGCACTGAGCACCCTCTGCCCCACTCCCGCCAGCATAAAAAACGGACACCCGAGGGTGTCCGTTCCCA
TAAGCTTTCGTGCTATCTGG

Product: EAL domain-containing protein

Products: NA

Alternate protein names: Blrp [H]

Number of amino acids: Translated: 254; Mature: 254

Protein sequence:

>254_residues
MDIPSAPPQLSCQQCQESGDLGFDFTMAFQPIIRCRTRTIFGYEALVRGINNEPAHSVISRVNDDNRYRFDQLCRIKAIA
LAAKLKMQGMLSINFLPNAVYRPERCIRTTLQAAREHQFPIEQIMFEFTEVERVADNQHIRHIVDHYQALGFKTAMDDFG
AGYSSLNLLADFLPDIIKLDMHLLRGIDKDKVRQAIVRHCLALFEELNITPLAEGIETRDEMACLQEMGIDLMQGYLFAR
PGFETLPEVDFVSL

Sequences:

>Translated_254_residues
MDIPSAPPQLSCQQCQESGDLGFDFTMAFQPIIRCRTRTIFGYEALVRGINNEPAHSVISRVNDDNRYRFDQLCRIKAIA
LAAKLKMQGMLSINFLPNAVYRPERCIRTTLQAAREHQFPIEQIMFEFTEVERVADNQHIRHIVDHYQALGFKTAMDDFG
AGYSSLNLLADFLPDIIKLDMHLLRGIDKDKVRQAIVRHCLALFEELNITPLAEGIETRDEMACLQEMGIDLMQGYLFAR
PGFETLPEVDFVSL
>Mature_254_residues
MDIPSAPPQLSCQQCQESGDLGFDFTMAFQPIIRCRTRTIFGYEALVRGINNEPAHSVISRVNDDNRYRFDQLCRIKAIA
LAAKLKMQGMLSINFLPNAVYRPERCIRTTLQAAREHQFPIEQIMFEFTEVERVADNQHIRHIVDHYQALGFKTAMDDFG
AGYSSLNLLADFLPDIIKLDMHLLRGIDKDKVRQAIVRHCLALFEELNITPLAEGIETRDEMACLQEMGIDLMQGYLFAR
PGFETLPEVDFVSL

Specific function: Binds to and releases the YcgE repressor from its bound DNA target in a blue-light-dependent (470 nm) fashion. A shift to low temperature also triggers a YcgF-medisted relief of repression by YcgE, suggesting YcgF may serve as a thermometer. Blue light ma

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 EAL domain [H]

Homologues:

Organism=Escherichia coli, GI1787410, Length=225, Percent_Identity=30.2222222222222, Blast_Score=115, Evalue=4e-27,
Organism=Escherichia coli, GI1787541, Length=140, Percent_Identity=38.5714285714286, Blast_Score=91, Evalue=6e-20,
Organism=Escherichia coli, GI87081980, Length=243, Percent_Identity=27.5720164609054, Blast_Score=88, Evalue=6e-19,
Organism=Escherichia coli, GI1788502, Length=245, Percent_Identity=27.3469387755102, Blast_Score=88, Evalue=6e-19,
Organism=Escherichia coli, GI1790496, Length=246, Percent_Identity=28.0487804878049, Blast_Score=85, Evalue=5e-18,
Organism=Escherichia coli, GI87081743, Length=237, Percent_Identity=28.2700421940928, Blast_Score=78, Evalue=6e-16,
Organism=Escherichia coli, GI87082096, Length=150, Percent_Identity=28.6666666666667, Blast_Score=78, Evalue=6e-16,
Organism=Escherichia coli, GI87081845, Length=252, Percent_Identity=26.1904761904762, Blast_Score=75, Evalue=3e-15,
Organism=Escherichia coli, GI1788849, Length=161, Percent_Identity=29.1925465838509, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI87081921, Length=229, Percent_Identity=24.4541484716157, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI226510982, Length=121, Percent_Identity=33.0578512396694, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1787055, Length=230, Percent_Identity=28.695652173913, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1786507, Length=131, Percent_Identity=28.2442748091603, Blast_Score=65, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001792
- InterPro:   IPR007024
- InterPro:   IPR001633 [H]

Pfam domain/function: PF04940 BLUF; PF00563 EAL [H]

EC number: NA

Molecular weight: Translated: 29030; Mature: 29030

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: PS50883 EAL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
6.7 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
6.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDIPSAPPQLSCQQCQESGDLGFDFTMAFQPIIRCRTRTIFGYEALVRGINNEPAHSVIS
CCCCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
RVNDDNRYRFDQLCRIKAIALAAKLKMQGMLSINFLPNAVYRPERCIRTTLQAAREHQFP
HCCCCCCHHHHHHHHHHHHHHHHHHHHHHCEEEECCCCCCCCHHHHHHHHHHHHHHCCCC
IEQIMFEFTEVERVADNQHIRHIVDHYQALGFKTAMDDFGAGYSSLNLLADFLPDIIKLD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
MHLLRGIDKDKVRQAIVRHCLALFEELNITPLAEGIETRDEMACLQEMGIDLMQGYLFAR
HHHHCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHCC
PGFETLPEVDFVSL
CCCCCCCCCCCCCC
>Mature Secondary Structure
MDIPSAPPQLSCQQCQESGDLGFDFTMAFQPIIRCRTRTIFGYEALVRGINNEPAHSVIS
CCCCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
RVNDDNRYRFDQLCRIKAIALAAKLKMQGMLSINFLPNAVYRPERCIRTTLQAAREHQFP
HCCCCCCHHHHHHHHHHHHHHHHHHHHHHCEEEECCCCCCCCHHHHHHHHHHHHHHCCCC
IEQIMFEFTEVERVADNQHIRHIVDHYQALGFKTAMDDFGAGYSSLNLLADFLPDIIKLD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
MHLLRGIDKDKVRQAIVRHCLALFEELNITPLAEGIETRDEMACLQEMGIDLMQGYLFAR
HHHHCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHCC
PGFETLPEVDFVSL
CCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]